| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,301,183 | A→C | 53.5% | intergenic (‑106/+3467) | recC ← / ← PP_RS08750 | RecBCD enzyme subunit RecC/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,301,183 | 0 | A | C | 53.5% | ‑3.4 / 3.1 | 11 | intergenic (‑106/+3467) | recC/PP_RS08750 | RecBCD enzyme subunit RecC/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base A (3/2); new base C (3/3); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
GTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTG‑CGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGG > KT2440/5301054‑5301313 | gTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTG‑CGGGGGCGGCCCGGCCCCCGCCCCCcgcgca > 8:178851/1‑133 (MQ=255) tgtTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTG‑CCGGCGCGGCCGTGCCCGCTCCCGCACCGAGTCGCGCACGTCgcg > 2:13501/1‑133 (MQ=255) aaGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTG‑CGGGCGCGGCCGTGCCCCCGCCCGCACCGCTTCGCGCCGGTCGC‑G‑TTTTTTTGCcac > 4:239765/1‑134 (MQ=255) aTGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCCGGGGGGGGCCGGGGCCCCGCCCCCCGCCTTAGGGGCGGGGGGG‑GTTTTTTTGCCCACAAACAAAAAAccaaacac > 5:104919/1‑129 (MQ=255) gTACATGAAACGGCCGAATGCCATCAGGCTG‑CGGGGGCGGGCGGGGCCCCTCCCCCCCCGCATCGCGCAGGGGGG‑G‑GTTTTTTGCCACCAATACAAAAACCCCACCAGGGGGCGCCGCTTCGGGTTTTTTGaaaa > 4:43399/1‑134 (MQ=255) gTACATGAAACGGCCGAATGCCATCAGGCTG‑CGGGGGCGGCCGGGCCCCCGGCCGCCGCGCATGGCGGCGGGGGAGG‑TTTTTTGCCC‑CAAAAACAAAAACCCAACCATCGGGGGCCGATTGGGGTTTTgggcaa > 8:103137/1‑129 (MQ=255) tG‑CGGCACCGCCCGTGCCGGCCCCGGCCGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATg < 4:84965/135‑1 (MQ=17) ggggggCCCGCGCCGGCCGCGATTCGCGCAGGGCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAAcccc < 6:152363/130‑1 (MQ=14) gcggggCCGGCTCCGGAAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAAcccc < 4:200472/130‑1 (MQ=14) ccggcccccGCCCCCCCCGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAAcccc < 5:309688/129‑1 (MQ=14) ccccggCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCAt < 4:183713/130‑1 (MQ=255) cGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCgg > 7:97644/1‑134 (MQ=255) | GTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTG‑CGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGG > KT2440/5301054‑5301313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |