Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,317,925 G→A 6.7% V245I (GTC→ATC)  CJ019_05003 → putative protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,317,9250GA 6.7% 179.1 / 2.0 59V245I (GTC→ATC) CJ019_05003putative protein
Reads supporting (aligned to +/- strand):  ref base G (22/33);  new base A (2/2);  total (24/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.22e-01

TGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTA  >  KT2440/5317791‑5318055
                                                                                                                                      |                                                                                                                                  
tgGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACg                                                                                                                                    >  8:49634/1‑135 (MQ=255)
tgGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACg                                                                                                                                    >  4:13247/1‑135 (MQ=255)
tgGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACg                                                                                                                                    >  5:256137/1‑135 (MQ=255)
       gcgcAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGc                                                                                                                             >  3:188949/1‑135 (MQ=255)
       gcgcAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGc                                                                                                                             >  5:372383/1‑135 (MQ=255)
              gCCAATGGCTTCATCCGGGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACAcc                                                                                                                      <  2:320194/135‑1 (MQ=255)
                  aTGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCGACCTTGATCGATGGCAAGGGGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCt                                                                                                                  <  2:114986/135‑1 (MQ=255)
                          aTCCGTGAGTGCCACGTTGACATCCTGCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGAATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGc                                                                                                           <  2:233372/134‑1 (MQ=255)
                          aTCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGcc                                                                                                          >  6:159107/1‑135 (MQ=255)
                          aTCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGc                                                                                                           <  2:98115/134‑1 (MQ=255)
                           tCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGCGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCa                                                                                                         >  8:306058/1‑135 (MQ=255)
                           tCCGTGAGTGCCACCGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCa                                                                                                         <  1:193141/135‑1 (MQ=255)
                            ccGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCAt                                                                                                        >  1:4057/1‑135 (MQ=255)
                            ccGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCAt                                                                                                        >  1:4876/1‑135 (MQ=255)
                            ccGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCAt                                                                                                        >  3:234296/1‑135 (MQ=255)
                                  gTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTTGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACAGCCTTCCTGGCCATGGACCt                                                                                                  >  1:111156/1‑135 (MQ=255)
                                  gTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCt                                                                                                  >  6:389349/1‑135 (MQ=255)
                                  gTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCt                                                                                                  >  2:68815/1‑135 (MQ=255)
                                  gTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCt                                                                                                  >  6:32156/1‑135 (MQ=255)
                                    gCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTg                                                                                                 >  2:876/1‑134 (MQ=255)
                                       aCGGTGACCTCCACCTGGGCAACGCCACCTTGCTCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAg                                                                                             <  6:208363/135‑1 (MQ=255)
                                       aCGGTGACATCCACCTGTGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAg                                                                                             <  4:90831/135‑1 (MQ=255)
                                       aCGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAg                                                                                             <  4:73328/135‑1 (MQ=255)
                                       aCGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAg                                                                                             <  7:393899/135‑1 (MQ=255)
                                        cGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACATCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGa                                                                                            >  2:79816/1‑135 (MQ=255)
                                        cGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACATCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGa                                                                                            >  2:85510/1‑135 (MQ=255)
                                           tGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACcg                                                                                         >  6:254552/1‑135 (MQ=255)
                                            gACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACATCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACcgc                                                                                        <  1:85510/135‑1 (MQ=255)
                                            gACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACATCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACcgc                                                                                        <  1:79816/135‑1 (MQ=255)
                                               aTCCACCT‑GGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGcc                                                                                    <  8:96973/135‑1 (MQ=255)
                                                tCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGcc                                                                                    >  1:308088/1‑135 (MQ=255)
                                                 ccACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCt                                                                                   <  5:32156/135‑1 (MQ=255)
                                                      tGGGCAACGCCACCTTGATCGAGGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGGCTACGCCGACACCGCCTGCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGt                                                                              <  1:336272/135‑1 (MQ=255)
                                                           aaCGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGc                                                                                     >  6:200081/1‑123 (MQ=255)
                                                           aaCGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGc                                                                                     <  5:200081/123‑1 (MQ=255)
                                                                cACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGCCACCGCCTTCCTGGCCATGGCCCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCg                                                                    >  2:365536/1‑135 (MQ=255)
                                                                       aTCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCtt                                                              <  3:60982/134‑1 (MQ=255)
                                                                       aTCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCt                                                               <  7:12920/133‑1 (MQ=255)
                                                                       aTCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCt                                                               <  5:159107/133‑1 (MQ=255)
                                                                          gATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTTGCCATGGCCCTGGAAGACCGCGGCCTGAAGTGCCTTGCCCGGCGCTtcaac                                                          >  2:381983/1‑133 (MQ=255)
                                                                          gATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTtca                                                            >  1:186332/1‑133 (MQ=255)
                                                                          gATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTtca                                                            >  6:189813/1‑133 (MQ=255)
                                                                            tGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAg                                                        <  5:118159/135‑1 (MQ=255)
                                                                            tGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAg                                                        <  2:122168/135‑1 (MQ=255)
                                                                                               gATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTg                                        <  4:388150/132‑1 (MQ=255)
                                                                                               gATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTg                                        <  2:308088/132‑1 (MQ=255)
                                                                                                aTTGCATCGAGTTCTACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACg                                     <  4:208274/134‑1 (MQ=255)
                                                                                                aTTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACg                                     <  6:249378/134‑1 (MQ=255)
                                                                                                 ttGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGAcc                                                                                          >  7:285652/1‑80 (MQ=255)
                                                                                                 ttGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGAcc                                                                                          <  8:285652/80‑1 (MQ=255)
                                                                                                       cGAGTTCAACGAGCCTTTCCGACTGAGCGACGTCTACGCCGACACCGCCTTCCTGGCGATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTa                             <  2:4876/135‑1 (MQ=255)
                                                                                                       cGAGTTCAACGAGCCTTTCCGACTGACGGACGGCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTa                             <  1:381983/135‑1 (MQ=255)
                                                                                                       cGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTa                             >  1:161361/1‑135 (MQ=255)
                                                                                                       cGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTa                             <  2:261466/135‑1 (MQ=255)
                                                                                                       cGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTa                             <  1:68815/135‑1 (MQ=255)
                                                                                                       cGAGTGCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTa                             <  5:5399/135‑1 (MQ=255)
                                                                                                                 gAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTg                   <  3:13247/135‑1 (MQ=255)
                                                                                                                       ttCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTg             >  8:114166/1‑135 (MQ=255)
                                                                                                                          cGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTc          <  2:276123/135‑1 (MQ=255)
                                                                                                                           gACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTc          <  3:303575/134‑1 (MQ=255)
                                                                                                                           gACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTc          <  1:876/134‑1 (MQ=255)
                                                                                                                                  cGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTa  <  4:234296/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
TGGAAAAGCGCAAGGCCAATGGCTTCATCCGTGAGTGCCACGGTGACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTA  >  KT2440/5317791‑5318055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑