Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,548,612	T→C	5.7%	D18G (GAT→GGT)	hcp1_2 ←	Protein hcp1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,548,612	0	T	C	5.7%	229.5 / 2.6	70	D18G (GAT→GGT)	hcp1_2	Protein hcp1
Reads supporting (aligned to +/- strand):  ref base T (39/27);  new base C (2/2);  total (41/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

CTTAGTGAAGGTGAAGTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAA  >  KT2440/5548479‑5548744                                                                                                                                      |                                                                                                                                     cTTAGTGAAGGTGAAGTTGGTGATTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGTCACCGAACTTGTATCCGATGATTTCGATCCAGTTGGCGTACTGCTTAt                                                                                                                                      >  6:347991/1‑134 (MQ=255) cTTAGTGAAGGTGAAGTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTAt                                                                                                                                      >  7:253516/1‑134 (MQ=255) cTTAGTGAAGGTGAAGTTGGTGAGTGTGGTCCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTAGGCACCGAACATGTAGCCGATGAGTTCGATCCAGTTGGCGTACTGCTTAt                                                                                                                                      >  7:311600/1‑134 (MQ=255)    aGTGAAGGTGAAGTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAg                                                                                                                                  >  7:264732/1‑135 (MQ=255)    aGTGAAGGTGAAGTTGGTGAGTGTGGTGCGGCCTGATGATCCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTAGTAGCCGATGATTTCGATCAAGTTGGCGTACTGCTTATCCAg                                                                                                                                  >  7:210600/1‑135 (MQ=255)      tgaaggtgaagTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTg                                                                                                                                >  5:328053/1‑135 (MQ=255)      tgaaggtgaagTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTg                                                                                                                                >  2:79017/1‑135 (MQ=255)      tgaaggtgaagTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTg                                                                                                                                >  5:369700/1‑135 (MQ=255)      tgaaggtgaagTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTg                                                                                                                                >  8:109881/1‑135 (MQ=255)      tgaaggtgaagTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTg                                                                                                                                >  2:375063/1‑135 (MQ=255)      tgaaggtgaagTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTg                                                                                                                                >  7:216402/1‑135 (MQ=255)      tgaaggtgaagTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATATCGATCCAGTTGGCGTACTGCTTATCCAGTg                                                                                                                                >  5:39428/1‑135 (MQ=255)      tgaaggtgaagTAGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTACCCGATGAATTCGATCCAGTTGGCGTACTGCTTATCCAGTg                                                                                                                                >  5:48620/1‑135 (MQ=255)           gtgaagTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCg                                                                                                                           <  8:253516/135‑1 (MQ=255)           gtgaagTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCg                                                                                                                           >  7:112727/1‑135 (MQ=255)                 ttGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTg                                                                                                                     >  1:336264/1‑135 (MQ=255)                   ggTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGAt                                                                                                                   >  8:319607/1‑135 (MQ=255)                     tGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGAttt                                                                                                                 <  8:60532/135‑1 (MQ=255)                         tgtgGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCcgc                                                                                                             <  1:254860/135‑1 (MQ=255)                         tgtgGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCcgc                                                                                                             <  6:307402/135‑1 (MQ=255)                           tggtgCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCcgcg                                                                                                            >  5:40773/1‑134 (MQ=255)                            ggtgCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGAttt                                                                                                                 >  2:99808/1‑128 (MQ=255)                            ggtgCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGAttt                                                                                                                 <  1:99808/128‑1 (MQ=255)                            ggtgCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGat                                                                                                          <  3:209389/135‑1 (MQ=255)                                    cTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACAt                                                                                                  <  5:192798/135‑1 (MQ=255)                                      gatgatGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATaa                                                                                                >  2:7558/1‑135 (MQ=255)                                      gatgatGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATaa                                                                                                >  5:88876/1‑135 (MQ=255)                                      gatgatGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATaa                                                                                                >  4:378620/1‑135 (MQ=255)                                      gatgatGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATaa                                                                                                >  8:4444/1‑135 (MQ=255)                                      gatgatGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCACTGCTTCGCGAGTGAGTTCCGCGATATGAACATaa                                                                                                >  1:287143/1‑135 (MQ=255)                                         gatgCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCa                                                                                             >  2:97847/1‑135 (MQ=255)                                           tgCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCAt                                                                                            <  7:28250/134‑1 (MQ=255)                                                cgcccgcGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAg                                                                                      >  7:221922/1‑135 (MQ=255)                                                cgcccgcGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAg                                                                                      >  2:24194/1‑135 (MQ=255)                                                cgcccgcGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAg                                                                                      >  8:246490/1‑135 (MQ=255)                                                cgcccgcGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCAAGTGATTTCCGCGATATGAACATAAGCATCGAAAg                                                                                      >  3:278848/1‑135 (MQ=255)                                                      cgGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGc                                                                                <  6:157335/135‑1 (MQ=255)                                                            ttGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACct                                                                          <  3:60199/135‑1 (MQ=255)                                                               cGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACctc                                                                         <  7:58122/133‑1 (MQ=255)                                                               cGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACctc                                                                         <  7:109881/133‑1 (MQ=255)                                                               cGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATCTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACctc                                                                         <  5:342891/133‑1 (MQ=255)                                                                ggTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTcc                                                                      >  1:232333/1‑135 (MQ=255)                                                                           tGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGt                                                           >  7:143511/1‑135 (MQ=255)                                                                            gCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGtt                                                          <  2:68679/135‑1 (MQ=255)                                                                             ctctGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTc                                                         >  1:32120/1‑135 (MQ=255)                                                                                   ttGGCACCGAACTTGTAGCCGATGTTTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGa                                                   <  2:78017/135‑1 (MQ=255)                                                                                   ttGGAACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGc                                                      <  8:216402/132‑1 (MQ=255)                                                                                       cACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAac                                               <  1:375063/135‑1 (MQ=255)                                                                                        aCCGAACTTGTAGCCGATGATTTCGATCCTGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGTTAAAGGTTCGGCTGATAacg                                              <  6:328053/135‑1 (MQ=255)                                                                                        aCCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAacg                                              >  3:377506/1‑135 (MQ=255)                                                                                          cGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAacga                                             >  1:347013/1‑134 (MQ=255)                                                                                          cGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTACCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACctc                                                                         >  2:344646/1‑106 (MQ=255)                                                                                          cGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTACCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACctc                                                                         >  2:339974/1‑106 (MQ=255)                                                                                          cGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTACCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACctc                                                                         <  1:339974/106‑1 (MQ=255)                                                                                          cGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTACCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACctc                                                                         <  1:344646/106‑1 (MQ=255)                                                                                               ttGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGt                                       >  7:222633/1‑135 (MQ=255)                                                                                                  tAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTc                                     <  6:152314/134‑1 (MQ=255)                                                                                                    gCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAg                                   >  2:309123/1‑134 (MQ=255)                                                                                                      cGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACa                                <  8:216475/135‑1 (MQ=255)                                                                                                       gatgatTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACAt                               <  2:32120/135‑1 (MQ=255)                                                                                                       gatgatTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACAt                               <  8:159124/135‑1 (MQ=255)                                                                                                       gatgatTTCGATCCAGTTGGCGTACTGCTTATCCAGGGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACAt                               <  2:336264/135‑1 (MQ=255)                                                                                                           atTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTc                           >  8:79749/1‑135 (MQ=255)                                                                                                           atTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTc                           >  1:63374/1‑135 (MQ=255)                                                                                                            tttCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCt                                                     >  1:102440/1‑108 (MQ=255)                                                                                                            tttCGATCCAGTTGGCGTACTGCTTATCCAGTGCTGCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCt                                                     <  2:102440/108‑1 (MQ=255)                                                                                                                      gTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCTATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGTTTCGGCTGATAACTACGATGTTCAGACATCGTCATCCTGAGGtt                >  7:230280/1‑135 (MQ=255)                                                                                                                          gCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTc            <  1:309123/135‑1 (MQ=255)                                                                                                                            gTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTcc           >  3:372329/1‑134 (MQ=255)                                                                                                                                    tATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAacg                                              <  6:50257/91‑1 (MQ=255)                                                                                                                                    tATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAacg                                              >  5:50257/1‑91 (MQ=255)                                                                                                                                    tATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTaaa  >  1:142866/1‑135 (MQ=255)                                                                                                                                    tATCCAGTGCTGCGCCAGTGATTTCCGCTATATTAAAATAAGCATCGAAACCCTTGCTAACCTCTCCCTGTTAACGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTaaa  <  6:341347/135‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     CTTAGTGAAGGTGAAGTTGGTGAGTGTGGTGCGGCCTGATGATGCGCCGCCCGCGGAGCTTGCGGTGGCAGAGGTGCTCTGGTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAA  >  KT2440/5548479‑5548744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑