Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,992,483 C→T 8.2% W166* (TGG→TGA)  ycaC_4 ← putative hydrolase YcaC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,992,4830CT 8.2% 146.7 / 3.7 49W166* (TGG→TGA) ycaC_4putative hydrolase YcaC
Reads supporting (aligned to +/- strand):  ref base C (21/24);  new base T (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01

TTGTTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGG  >  KT2440/5992355‑5992614
                                                                                                                                |                                                                                                                                   
ttgttgGCGTTGTAGGCGTTGTAGCTGGTCTGCAGGTTGCGGTAGTCAGGTATGTGGTTGGAGCACTGCGCGGCCAGGCCTTAGATGTCGTTGCGCCAGTCGCTGTGCAGCTCACAGGCCACGCCGAACCAGGt                                                                                                                                <  7:360340/134‑1 (MQ=21)
ttgttgGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGt                                                                                                                                <  6:136779/134‑1 (MQ=255)
ttgtggGCGTTGCAGGCGTTGTAGCTGGTCCTCAGGTTGCGGTAGTCCGGGAGGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGt                                                                                                                                <  5:36474/134‑1 (MQ=255)
      gcgcTGAAGGCGTTGTAGCTGGTCATCAGGGTGCGGTAGTCCGGGATGTGGATGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACATGCCACGCCGAACCAGGTCATCAg                                                                                                                          <  1:233748/134‑1 (MQ=255)
      gCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGt                                                                                                                         >  2:297979/1‑135 (MQ=255)
       cGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACCGCTCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCCCAGGCCACGCCGAACCAGGTCATCAGtt                                                                                                                        >  8:277704/1‑135 (MQ=255)
       cGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGAGGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGtt                                                                                                                        <  7:288695/135‑1 (MQ=255)
       cGTTGAAGGCGTTGTAGCTGGTCATCAGGTGGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGtt                                                                                                                        <  2:180863/135‑1 (MQ=255)
        gTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTg                                                                                                                       >  8:147840/1‑135 (MQ=255)
         ttGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTgc                                                                                                                      <  7:391260/135‑1 (MQ=255)
         ttGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTgc                                                                                                                      <  8:309476/135‑1 (MQ=255)
          tGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTgcg                                                                                                                     <  3:366526/135‑1 (MQ=255)
          tGAAGCCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTgcg                                                                                                                     <  3:26968/135‑1 (MQ=255)
              ggCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCAcc                                                                                                                 <  5:365988/135‑1 (MQ=255)
                     tAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTgg                                                                                                          >  3:373500/1‑135 (MQ=255)
                      aGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGc                                                                                                         >  8:297698/1‑135 (MQ=255)
                        cTGGTCATCAGGGTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTc                                                                                                       <  1:360176/135‑1 (MQ=255)
                             catcaGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCg                                                                                                  >  6:315876/1‑135 (MQ=255)
                              atcaGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCgg                                                                                                 >  1:27577/1‑135 (MQ=255)
                                caGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCgggc                                                                                               >  8:376702/1‑133 (MQ=255)
                                          tAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGgcggcg                                                                                     <  2:27577/135‑1 (MQ=255)
                                          tAGTCCGGGATGTGGGTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGATGCGCACCTGCCTGGCTCATGCGGTCGTGGgcggcg                                                                                     <  1:207736/135‑1 (MQ=255)
                                                   aTGTGGTTGGCGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTc                                                                            <  6:241452/135‑1 (MQ=255)
                                                   aTGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTc                                                                            <  7:297698/135‑1 (MQ=255)
                                                       ggTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATg                                                                         <  7:292270/134‑1 (MQ=255)
                                                         ttGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGt                                                                                    >  8:165322/1‑121 (MQ=255)
                                                         ttGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGt                                                                                    <  7:165322/121‑1 (MQ=255)
                                                         ttGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCg                                                                      >  7:199541/1‑135 (MQ=255)
                                                               cacaGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCTTGCGGTCGTGGGCGGCGGCCGGGGTGATGGCgggggag                                                                >  1:163586/1‑129 (MQ=18)
                                                                     gcgGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCg                                                          <  8:199541/135‑1 (MQ=255)
                                                                      cgGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTAcc                                                           >  7:328575/1‑133 (MQ=255)
                                                                            ggCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAATCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCAt                                                   <  3:48687/135‑1 (MQ=255)
                                                                                ttCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGt                                               <  8:328575/135‑1 (MQ=255)
                                                                                ttCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGt                                               <  7:208794/135‑1 (MQ=255)
                                                                                        cGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCATGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACg                                        <  3:180078/134‑1 (MQ=255)
                                                                                          ttGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAATCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCAt                                                   <  3:48688/121‑1 (MQ=255)
                                                                                          ttGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAATCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGGCATGGCGTTGAAGGTACCGGAGGCAt                                                   >  4:48688/1‑121 (MQ=255)
                                                                                          ttGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAATCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGGCATGGCGGTGCAGGTTCCGGAGGCCTCAGTGACCACGAac                                     >  4:48687/1‑135 (MQ=18)
                                                                                          ttGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGGACCGGAGGCATCAGTGACCACGAac                                     >  3:127025/1‑135 (MQ=255)
                                                                                          ttGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGAGGGGCATGGCGTTGAAGGTACCGGAGGCATCAGTGCGCCCGAac                                     >  6:171392/1‑135 (MQ=18)
                                                                                           tGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGGGGGTCATGGCGTTGTAGGTACCGGAGGCATCAGTGCCCACGAaca                                    >  2:324164/1‑135 (MQ=255)
                                                                                              gcCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGt                                 <  5:315876/135‑1 (MQ=255)
                                                                                                     gcgGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACCCCTCGAAcac                          >  4:134839/1‑133 (MQ=255)
                                                                                                     gcgGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGGTGAAGGTTCCGGAGGCCTGAGTGACCACCAACCCGTCGAActg                          >  8:313457/1‑134 (MQ=21)
                                                                                                           cAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCttcttc                    >  6:87531/1‑135 (MQ=255)
                                                                                                                      ccACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGCCCACGCACACGTCGCACTCTTCTTCCAGGGCCGACa         >  3:165456/1‑135 (MQ=255)
                                                                                                                           ccGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGGCCACGAACACGTCGCCCTCTTCTTCCAGGGCCGACAGGGcc    >  8:87297/1‑135 (MQ=255)
                                                                                                                            cGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGGCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCg   <  3:355435/135‑1 (MQ=255)
                                                                                                                             gAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTGATGGCGGTGAAGGTACCGGAGGGATCAGTGCCCCCGAACCCGTCGCCCTCTTCTTCCAGGGCCGCCCGGGCCgg  >  2:248877/1‑135 (MQ=25)
                                                                                                                                |                                                                                                                                   
TTGTTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGG  >  KT2440/5992355‑5992614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑