| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 6,016,995 | T→G | 51.9% | intergenic (+75/+69) | puuB_8 → / ← CJ019_05632 | Gamma‑glutamylputrescine oxidoreductase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 6,016,995 | 0 | T | G | 51.9% | ‑4.6 / 2.4 | 19 | intergenic (+75/+69) | puuB_8/CJ019_05632 | Gamma‑glutamylputrescine oxidoreductase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base G (2/8); total (5/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCT‑G‑TGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGA > KT2440/6016871‑6017122 | cACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGCCGCGCCCCCTGG‑TGGGGGCGGGGGGGccccggcc > 1:310774/1‑130 (MQ=255) cTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCT‑G‑TGGGGGCGGGCGGGCCCGCGCCCCCCCGGTCggg > 6:151818/1‑134 (MQ=255) aaTGCTGTGGCATCCCCGAAACCCGCTGACTTCAACGCCCCAAGCCTTGGGGCACCAAGGGGCTTGATTAAAAGCAACACGCGGACGCGCCCCGT‑G‑GGGGAGCGGGCGGGCCCGCCCCCACAGGGTCGGTGCAAg < 8:89277/135‑1 (MQ=255) aTCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCAAAACGCTGACGCGCCCCCT‑G‑TGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTg < 4:115743/135‑1 (MQ=255) gagCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCT‑G‑TGGGGGCGGGCGTTCCCGCGCCCCCCGGGGCGGTGGCAGTTTCAAGTCTGGGGACCAACAg > 7:231912/1‑135 (MQ=255) aCTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCT‑G‑TGGGCGCGGGCGTGCCCCCGCCCACCGGGTCGGGGGAAGTTTCAAAGCTGGGGGACCACACGGCCTc > 7:121298/1‑135 (MQ=255) gttGAAAGTCACAAT‑CGTGTGCAAGAAACTGTATTGCTAAAAAAAAAAACGCTGACGCGCCCCCT‑G‑GGGGAGCGGGCGGGCCGGATCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCa < 8:162987/134‑1 (MQ=255) cTTCAACGTCCCCATCATGGTGCAACAAAGTGTATTCATAAAAAACAAAATGCTGCCGCGCCCCCG‑G‑TGGGCGCGGGCGTGCCCGCCCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTcc < 1:234745/135‑1 (MQ=255) acgcccccATCCTTGTGGCAGCAAGTGTCTTGCTTAAAATCAAAACGCTGCCGCGCCCCCT‑G‑TGGGAGCGGGCGTGCCCGCGCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGa < 3:43833/131‑1 (MQ=255) aTCCTGGTGGCAGAAACTGTCTTGCTTAAAATATAAATGCTGCCGCGCCCCGT‑G‑GGGGAGCGGGGGGGCCCGCTCCAACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtg < 7:136504/135‑1 (MQ=255) ccTTGTGCCAGCAACTGTCTTGCTTAAAATCAAAACGCTGCCGCGCCCCCT‑G‑GGGGAGCGGGCGGGCCCGCCCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTc < 6:369161/135‑1 (MQ=255) ccTTGGGGCAGCAACTGTCTTCCTTAAAATCTAAAAGCTGACGCTCCCCCT‑G‑TGGGAGCGGGCGTGCCCGCTCCAACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTc < 8:106998/135‑1 (MQ=255) tggtgGCCGAAACTTTCTGCCTTAAAATAAAAACCCTGACGCGCCCCCT‑G‑TGGGCGCGGGCGGGCCCGCGCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGAAACAACAGGCCCTCCAGCGACCATCGTGTCAg < 2:220386/133‑1 (MQ=255) aCTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCT‑GTTGGGCGCGGGGGTGCCCGGGCCCCCAGGGGGGGGGCAATTTTCAAGGGGGGGGGACCAAAGGCCCGCCCGCCCCCACCGGGGCAGGCCTTCCCGa > 8:320065/1‑135 (MQ=255) aaaTCTAAACGCGGCCGCGCCCCCT‑G‑TGGGAGCGGGCGGGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGcc < 4:121576/133‑1 (MQ=255) gcggccgcgCCCCCT‑G‑GGGGAGCGGGCGGGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACCACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCggg < 8:119544/130‑1 (MQ=255) ccccT‑G‑GGGGCGGGGGCGGGCCCGCCCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCa < 1:49688/135‑1 (MQ=255) aGCGGGCGTGCCCGCGCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCg < 8:41561/135‑1 (MQ=255) gCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGa < 3:339250/135‑1 (MQ=255) | CACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCT‑G‑TGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGA > KT2440/6016871‑6017122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |