Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,021,519 A→C 36.0% L58R (CTG→CGG)  ycaD_4 ← putative MFS‑type transporter YcaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,021,5190AC36.0% 22.1 / 2.4 36L58R (CTG→CGG) ycaD_4putative MFS‑type transporter YcaD
Reads supporting (aligned to +/- strand):  ref base A (4/13);  new base C (12/2);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 9.65e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGC  >  KT2440/6021385‑6021653
                                                                                                                                      |                                                                                                                                      
cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCa                                                                                                                                        >  7:332012/1‑135 (MQ=255)
cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCa                                                                                                                                        <  3:367656/135‑1 (MQ=255)
     atcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTg                                                                                                                                   <  7:152300/135‑1 (MQ=255)
     atcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGcgg                                                                                                                                   >  6:195913/1‑133 (MQ=255)
      tcaGCCGCAGCAGCAGCCATACCGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTg                                                                                                                                   <  3:317241/134‑1 (MQ=255)
         gCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGgcg                                                                                                                                <  6:276838/134‑1 (MQ=255)
             cagcagcagCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGc                                                                                                                           <  7:318846/135‑1 (MQ=255)
                    agcCTTCACGGGTAACTCGGCCGCAGGGCGTGCTGGCCAATCGACCGGGCCCCGCCCCACTGGCACCGGCGCTTGGCCGAAGGTAGGCCAACCCAACCTGCCAGGGGGGGGGCCTGGCGGGCGCCGGCCAGTACg                                                                                                                    <  6:72906/135‑1 (MQ=255)
                    agcCATACCGGGAAACTCGGCAGCAGCGCTAGCTGGCAAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCTAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACg                                                                                                                    <  3:329037/135‑1 (MQ=255)
                                   cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCTCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCAAACCTGCCAGGCGGCTGGACAAGCTGGCGCCCGACAGGACGCCCAACCCCCGCAtt                                                                                                     >  2:340680/1‑135 (MQ=255)
                                   cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCGACCCTGGCGCCGCCCCCGAAGCCCACCCCCCGCAtt                                                                                                     >  1:251529/1‑135 (MQ=255)
                                   cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCGCCGGCCCGTACGCCCAACGCCGGCAtt                                                                                                     >  8:71854/1‑135 (MQ=255)
                                   cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCAtt                                                                                                     >  4:326970/1‑135 (MQ=255)
                                   cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCTGGCGCCCGCCCCGACCCCCAAccccccccat                                                                                                     >  5:64810/1‑129 (MQ=255)
                                   cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCAGGACCGGCAGACGCCGGCCCGCAAGCCCCACGCCGGCAtt                                                                                                     >  2:345321/1‑135 (MQ=255)
                                     aGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCGGGCGCCGGCCCGTAACCCCAACGCCGGccatgc                                                                                                   >  7:391295/1‑130 (MQ=255)
                                                        ccAACCCACAGCCCCCCCCCCCCCAGCCACAGGCCCAGCGCCGAAGGTACGCCAACCAACCCTGCCAGGCGGCTGGCCTGGTGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCg                                                                                <  5:355539/135‑1 (MQ=255)
                                                        ccAAACCACCGCCCCCCGCCCCCCCGGAACTGCCGCATCGCCTACGCTACGCCAACCCAACGTGCCAGGGGGCGGGCCCGGCGGGCGCCGGCCTGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCg                                                                                <  2:45026/135‑1 (MQ=255)
                                                              gACCGCGCCCCGCCCCCCCGGACCCGGCGATGCGCCTATGGACCCCCACCCCACGCGGCCTGCGGGCTGGCCCGGCGGGGGCCGCCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTg                                                                          <  2:360135/135‑1 (MQ=255)
                                                               aCAGCGCCCCGCCCCACCGGCACAGGCGCAGCGCCGAAGGTACGCCACCCCACCCTGCCCGGCGGCGGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGc                                                                         <  5:195913/135‑1 (MQ=255)
                                                                    gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCGGGCGCCGGCCCGTACGCCCCACCCCGGCATTGCCGCCCACACGCCGATGGCGCAGCTGCCCTAg                                                                    >  4:341141/1‑135 (MQ=255)
                                                                    gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCAGGCCCCGCAGGCCCCGGCCAGGTCCCCCCACGCCGGCCTTGCCGCCCACACCCCGATGGCGAAGCGGCCCTat                                                                    >  8:376210/1‑134 (MQ=255)
                                                                     ccccgccccACAGGCAACGGCGCATCGCCGACGGTACGCCACCCCACCCTGCCAGGGGGCTGGCCTGGCTGGCGCCGGCATGTACGCCAAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGTCGTAGc                                                                   <  6:71960/135‑1 (MQ=255)
                                                                      cccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGGCCGGCGGGCGCCGGCCCGGACGCCCCACCCCCGCAGTGGCCCCCACACGCCCCAGGGGACGCAGCCGGAGcc                                                                  >  4:11797/1‑135 (MQ=255)
                                                                       ccgccccACCGGCACCGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGccc                                                                 <  1:232421/135‑1 (MQ=255)
                                                                        cgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGcccc                                                                <  8:326544/135‑1 (MQ=255)
                                                                        cgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGGCCCGCGGGCGCCGGCCCGGACGGCCCACCCCCGCCGTGCCGCCCTCCCCCCGCGGGCGGAGCGGCCGTAGcccc                                                                >  8:28726/1‑135 (MQ=255)
                                                                           cccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGGCAGGCTGGCGCCGGCCAGGACGCCCAACGCCGGCCTTGGCGCCCAGACCCCGATGGGGGCGCTGGCGTAGCCCCCgg                                                             >  6:135052/1‑135 (MQ=255)
                                                                            ccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCCGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGc                                                            <  4:30615/135‑1 (MQ=255)
                                                                                        gcgcATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCAGGCGCCGGCCAGAACCCCCAACCCCCGCATTGCCCCCATCACCCCGATGGCGAAGCAGGCGGAGCCCCCGGCCGCCAGGCGCAg                                                >  4:236465/1‑135 (MQ=255)
                                                                                             tCGCCGACGGTCCGCCACCCCAACCGGCCAGGCGGCGGGCCTGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGaca                                           <  6:387705/135‑1 (MQ=255)
                                                                                             gcgcgGACGGTACGCCAACCCACCCTGCCGGGGGGCGGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGaca                                           <  4:219967/134‑1 (MQ=255)
                                                                                                 cGACGGTACGCCAACCCACCCTGCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAg                                       <  7:28726/135‑1 (MQ=255)
                                                                                                                 caaccTGCCAGGCGGCTGGCCCGGCTGGCGCCGGCCAGTACGCCCCACGCCCGCATTGCCGCCAACACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGccc                       >  7:83264/1‑134 (MQ=255)
                                                                                                                 caaccTGCCAGGCGGCTGGCCAGGCTGGCGCCCGCCACTACCCCCAACGCCGGCATAGCCCCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCACGCCTACAGGCGCAGGGACACCAGCGGCATGGTCACGCCa                       >  8:275194/1‑135 (MQ=255)
                                                                                                                   cccTGCCCGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  7:8233/134‑1 (MQ=255)
                                                                                                                           ggcggcTGGCCTGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGcc             <  1:288711/135‑1 (MQ=255)
                                                                                                                                 tGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGacac       <  6:144120/135‑1 (MQ=255)
                                                                                                                                      aGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGc  <  8:332012/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGC  >  KT2440/6021385‑6021653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑