Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,178,247 G→C 24.9% L614V (CTG→GTG)  mnmG ← tRNA uridine 5‑carboxymethylaminomethylmodification enzyme MnmG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,178,2470GC24.9% 38.4 / 6.1 47L614V (CTG→GTG) mnmGtRNA uridine 5‑carboxymethylaminomethylmodification enzyme MnmG
Reads supporting (aligned to +/- strand):  ref base G (14/19);  new base C (2/10);  total (17/30)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAAGCTGGCGCGCACCTGTGGACAACTCTTCAGCGTGTTGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAGTGAATCAGCAACAGGGAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCTGTATCTCCTTGGACAGGCCGGAAATCGTCGTGTAGTCGATATC  >  KT2440/6178136‑6178376
                                                                                                               |                                                                                                                                 
ccACGCGGGCGCGCACCTGTGGACAACTCTTCAGCGTGTTGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAGTGAATCAGCAACAGGGAAATCGCCGCCgggg                                                                                                            <  8:3296/135‑1 (MQ=255)
 cAAGCTGGCGCGCACCTGTGGACAACTCTTCAGCGTGTTGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGGTTTTTAAAGTGAATCAGCCAAACGGAAATCGCCCCCggggg                                                                                                           >  2:267437/1‑134 (MQ=255)
    gcgggCGCGCACCTGTGGACAACTCTGCCGCGTGTTGCGGGGGCACCCGGGAACTCAAGCGCTTTGCACCAATGCGCGGCCAGCGCCGCGTTTTTTCAAGTCAATCAGCAACCGGGAAATCGCCGCCGGGGTcac                                                                                                        <  2:65175/132‑1 (MQ=255)
         cgcgcACCTGTGGACAACTCTGCAGCGTGTTGAGGGGTCACCAGGGAACTCACGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAGTGAATCAGCAACAGGGAAATCGCCGCCGGGGTCACACCCg                                                                                                   <  8:244059/135‑1 (MQ=255)
             ccccTGTGGACAACTCTGCCGCGTGTGGCGGGGTGACCAGGCAACTCACGCGCTTTGCCCCAATGCGCGGCCCGCGCCGGGTTTTTTCAAGTGAATCCGCAACCGGCAAAGCGCCGCCGGGGTCACACCCGGGAt                                                                                               <  8:82314/133‑1 (MQ=255)
               ccTGGGGACACCTCTTCAGCGTGTTGCGGGGTGACCAGGCAACTCAAGCGCTTTGCTCCAATTCGCGGCCCGCGCCGCGTTTTTTCAAGTGAATCACCAACCGGCAAATCGCCGCCGGGGTCACACCCGGGATAc                                                                                             <  7:195488/135‑1 (MQ=255)
                   tgGACACCTCTTCCGCGGGTGGGGGGGGGACCCGCGAACACACGCGCTTTGCGCCAATGCGGGGCCCGGGCCGCGTTTTTTCACGTGAATCAGCACCCGGGAACTCGCCGCCGGGGTCACACCCGGGATACGCGa                                                                                         <  1:20955/135‑1 (MQ=255)
                     gACACCTCTTCAGCGGGTGGCGGGGGGACCAGGGAACCCAAGCGCTTTGCACCAATTCGCGGCCCGCGCCGCGTTTTTTCAAGTGAATCACCAACAGGGAAAGCGCCGCCGGGGTCACACCCGGGATACGCGAAg                                                                                       <  7:288793/135‑1 (MQ=255)
                     gACAACTCTTCAGCGTGTTGAGGGGTGACAAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGGTTTTTTAATTGTATCAGCAACAGGGAAAACGCCGCCGGGGGGACCCCCGGGATACGCCAAg                                                                                       >  7:73311/1‑135 (MQ=255)
                      aCAACTCTTCAGCGTGTTGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAGTGAATCAGCAACAGGGAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAg                                                                                       >  3:255509/1‑134 (MQ=255)
                           tctTCAGCGTGTTGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCTATTCGCGGCCAGCGCCGCGTTTTTTAAATTGAATCAGCAACAAGGAAAAACCCCCCGGGGTGACACCCCGGATAACCCAAGCCTGaa                                                                                 >  8:251097/1‑134 (MQ=255)
                           tctTCAGCGTGTTGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAATGAATCAGCAACAGGGAAAACGCCGCCGGGGGCACACCCCGGATAAGCCAAGCCAGAc                                                                                 >  3:104027/1‑135 (MQ=255)
                            ctTGAGCGTGTTGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAGTGAATCAGCAACAGGGAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGAcc                                                                                >  7:9169/1‑135 (MQ=255)
                             ttCAGCGTGTTGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTGTTTTAAATTGAATCAGAAAAAAGGAAATAGCCCCCCGGGTCACACACCGGAAACGCCAACCCAGAcac                                                                               >  4:60577/1‑133 (MQ=255)
                             ttCAGCGTGTTGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGGTTTTTGTAGGGAATCAGGCACAGGGTAATCGCCGCCGGGGGCCCACCCGGGCTACGCGACGCCTGAccc                                                                               >  2:287218/1‑135 (MQ=255)
                                aGCGTGTTGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAATGAATAACCAACACGGAAATCCCCCCCCGGGTCCCACCCCGGCAACGCCAAGCCAGACCCac                                                                             >  3:180587/1‑133 (MQ=255)
                                       tGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAGGGAATCAGCAACAGGGAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCg                                                                     <  8:9169/135‑1 (MQ=255)
                                       tGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGGTTTTTTAAATGAATGAGCAACAGGGCAAAAGCCCCCGCGGTGACACCCCGGCAAAGCCAACCCAGACCCAGCCTCAGCg                                                                     >  3:281134/1‑135 (MQ=255)
                                       tGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGAGTTTTTTCAAGTGAATCCCCAACAGGGAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGc                                                                      <  4:338723/134‑1 (MQ=255)
                                        gAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTTAAGTGAATCAGCAACAAGGAAATAGCCCCCCCGGTGACCCCCCGGCTACGCGAACCCAGACCCCGCGTcgtcgg                                                                    >  3:44804/1‑130 (MQ=255)
                                        gAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTGTTTTGTAGTGAATAATCAAACCGGAAAACGCCGCCCGGGGCACCCCCCGGCTACGCGAAGCCAGACCCCGCCTCGGccg                                                                    >  4:65234/1‑133 (MQ=255)
                                         aGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAGTTAATACCCAACAGGGAAATAGCCGCCGGGGTCCCACCCGGGATACGCCAACCAAGACCCACAGTCAGCggc                                                                   >  4:366849/1‑134 (MQ=255)
                                           gggTCACCCGGCAACTCACGCGAGTTGCCCCAATTCCCGGCCACCGCCGCGTTTTTTCACGAGAAACAGCAACAGGCAAACCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACg                                                                 <  7:136614/135‑1 (MQ=255)
                                                 ccAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGGTTTTTGAAGTGAATCAGCAACAGGGAAATCTCCGCCCGGGGCACACCCGGGCTAAGCGCAGCCAGACCCACCGGCTGCGGACgggggtt                                                           >  8:164247/1‑131 (MQ=255)
                                                     ggAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAGTGAATCAGCAACAGGGAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTc                                                       <  8:267294/135‑1 (MQ=255)
                                                        acccacgcgcTTTGCGCCAATTCGCGCCCCGCGCCGGGTTTTTCCAAGTCAACCCCCAACCGGGAAACCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGc                                                    <  3:114624/129‑1 (MQ=255)
                                                        aCTCACGCGCTTTGCACAAATACGCGGCCCGCGCGGCTTTTTTTCACGTAAATCAGAAACTGGCAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGc                                                    <  3:126839/135‑1 (MQ=255)
                                                          tCACGCGCTTTGCCCAAATTCGCGGCCCGCGCCGCTTTTTTTCACGTGAATCAGCAACCGGCAAACCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCtt                                                  <  1:267437/135‑1 (MQ=255)
                                                          tCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAGTGAATCAGCAACAGGGAAAGCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCtt                                                  <  7:132625/135‑1 (MQ=255)
                                                               cgttttGCCCAAATGCGCGGCCGGCGCGGGTTTTTTTCATTTGAATCACCAACCGGCAAACCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCt                                             <  7:351601/132‑1 (MQ=255)
                                                               cgcTTTGCTCCAATTCGCGGCCAGCGCCGGGTTTTTTCATGTCAAGCCGCAACAGGGAATTCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCt                                             <  6:81387/135‑1 (MQ=255)
                                                               cgcTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAGTGAATCAGCAACAGGTAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCt                                             <  2:128041/135‑1 (MQ=255)
                                                               cgcTTTGCCCAATTTCGCGGCCCGGGCGGTTTTTTTTCATGTAAACCCCAAACCGGCAAACCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCt                                             <  4:2382/135‑1 (MQ=255)
                                                               cgcTTGGCGCAAATGCGCGGCCCGCGCCGCTTTTTTTCAAGTGACTCAGCCACCGGCAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCt                                             <  4:270735/135‑1 (MQ=255)
                                                                 ttttGCTCTTATTCGCGGGAAGCGCCTGGTTTTTTCAAGTGATGGATTAAAAGGCTACTCGCCGCCGGGGTCACACCCGTGATACGCGAAGCGTGAGCCAGCGTCTGCTGACGGGTCTGGCTCAGCTTGCCCt                                             <  2:143512/132‑1 (MQ=255)
                                                                       tCCAATTCGCGGCAAGCGCCGCGGTTTTTCAAAGGTAAAAGAAAACAGGAAATCGCCACCGGGGTAAAACCCGGGATACGCGAAGCCTGAAACACCGTCTGCGGTAGGGGGTGGGTACGCTTGCCCTGGAtcaac                                     >  2:41424/1‑132 (MQ=255)
                                                                         cAATTCGGGGCCAGCGCCGCTTTTTTTCATTTTAATCACCAACTGGGAATCCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCTGTATCTCCtt                                   <  4:180587/135‑1 (MQ=255)
                                                                          aaTGCGCGGCCAGCGCCGGGTTTTTTCAAGTCAATCAGCAACAGGTAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGTGTCTGTCTCAGCTTGCCCTGTATCTCCtt                                   <  8:113553/134‑1 (MQ=255)
                                                                             gcgcgCCCAGCGCCGGGTTTTTTCACGTGAATCACCACCCGGCAAAGCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCTGTATCTCCTTGGAc                               <  3:235539/134‑1 (MQ=255)
                                                                                 ggCCAGCGCCGCGGTTTTTGAAGTTAATCAGCAAAAGGGGAATAGCCGCCGGGGTCACCCCCCGGATACGCGAAGCCTGACCCAGCGGCTGCGGGCGGGTGTGGCTCAGCTTGCCCTGTATCTCCGTTGACAGGc                           >  4:172262/1‑135 (MQ=255)
                                                                                  gCCAGCGCCGCGTTTTTTCAAGTGAATCAGCAACAGGGAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCTGTATCTCCTTGGACAGGcc                          <  4:255509/135‑1 (MQ=255)
                                                                                       cgccgcGTTTTTTCAAGTGAAGCCGCACCAGTGAATGCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCTGTATCTCCTTGGACAGGCCGGaaa                     <  8:39753/135‑1 (MQ=255)
                                                                                            ggTTTTTTCAAGTGAATCACCAATTGGTAAATCGCCGCCGGGGTCACACGCGTGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGTCTCAGCTTGCCCTGTATCTCCTTGGACAGGCCGGAAAtcgtc                <  7:326102/134‑1 (MQ=255)
                                                                                                ttttAAAGTGAATCACCAACAGGTAAACCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCTGTATCTCCTTGGACAGGCCGGAAATCGTCgtg             <  7:251097/134‑1 (MQ=255)
                                                                                                   tCAAGTGAATCAGCAACAGGGAAATCGCCGCCGGGGGCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCTGTATCTCCTTGGACAGGCCGGAAATCGTCGTGTAg          <  2:122229/134‑1 (MQ=255)
                                                                                                        tGAATCAGCAACAGGGAAAGCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCTGTATCTCCTTGGACAGGCCGGAAATCGTCGTGTAGTCGata    <  5:152428/135‑1 (MQ=255)
                                                                                                          aaTCAGCAACAGGGAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCTGTATCTCCTTGGACAGGCCGGAAATCGTCGTGTAGTCGatata  >  6:365898/1‑134 (MQ=255)
                                                                                                               |                                                                                                                                 
CCAAGCTGGCGCGCACCTGTGGACAACTCTTCAGCGTGTTGAGGGGTGACCAGGGAACTCAAGCGCTTTGCTCCAATTCGCGGCCAGCGCCGCGTTTTTTCAAGTGAATCAGCAACAGGGAAATCGCCGCCGGGGTCACACCCGGGATACGCGAAGCCTGACCCAGCGTCTGCGGACGGGTCTGGCTCAGCTTGCCCTGTATCTCCTTGGACAGGCCGGAAATCGTCGTGTAGTCGATATC  >  KT2440/6178136‑6178376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑