Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 258,568 T→C 5.8% L216P (CTG→CCG)  davT → 5‑aminovalerate aminotransferase DavT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440258,5680TC 5.8% 227.3 / 3.0 69L216P (CTG→CCG) davT5‑aminovalerate aminotransferase DavT
Reads supporting (aligned to +/- strand):  ref base T (42/23);  new base C (2/2);  total (44/25)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CCGAGCGAACTGCACGGTATCAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATGGAACAGATGGGCGTTG  >  KT2440/258438‑258702
                                                                                                                                  |                                                                                                                                      
ccGAGCGAACTGCACGGTATCAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCa                                                                                                                                    >  8:56342/1‑135 (MQ=255)
 cGAGCGAACTGCACGGTATCAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAg                                                                                                                                   >  7:290549/1‑135 (MQ=255)
      gAACTGCACGGTATCAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGcc                                                                                                                               >  2:75964/1‑134 (MQ=255)
        aCTGCACGGTATCAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCg                                                                                                                              >  4:41123/1‑133 (MQ=255)
        aCTGCACGGTATCAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCg                                                                                                                              >  8:4785/1‑133 (MQ=255)
        aCTGCACGGTATCAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCg                                                                                                                              >  7:294567/1‑133 (MQ=255)
        aCTGCACGGTATCAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCg                                                                                                                              >  7:299597/1‑133 (MQ=255)
         cTGCACGGTATCAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAACAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGaaa                                                                                                                           <  1:339957/135‑1 (MQ=255)
                    cAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGaa                                                                                                                <  8:58074/135‑1 (MQ=255)
                    cAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGaa                                                                                                                >  6:192372/1‑135 (MQ=255)
                      gCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAgc                                                                                                              <  4:156728/135‑1 (MQ=255)
                         ttGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGcc                                                                                                           >  8:255501/1‑135 (MQ=255)
                              gccgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  4:288973/3‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGCCGCCGCGCCGCGCGCCATCGCCGCAATCCTCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  5:320918/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGCCGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGACCTGATGAAGAGCCTgcgc                                                                                                      >  6:60482/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGCCATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  7:220873/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGCAGCGCCTgcgc                                                                                                      >  3:258847/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  2:206594/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  5:291752/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  5:141229/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  5:138034/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  4:264665/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  4:176375/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  1:316638/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  2:22003/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGACAGCGCCGAAAGAGCTGATGAAGCTCCTgcgc                                                                                                      >  1:374079/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCCGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  7:121274/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCCGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  7:116182/1‑135 (MQ=255)
                              gacgCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCATCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTgcgc                                                                                                      >  3:14842/1‑135 (MQ=255)
                                  caacccccTCGGTCTAGCGCACATTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCAAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGccc                                                                                                  <  2:374079/129‑1 (MQ=255)
                                    aTCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCtg                                                                                                >  3:63441/1‑135 (MQ=255)
                                     tCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCtgt                                                                                               <  4:106386/135‑1 (MQ=255)
                                                 aGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACg                                                                                   <  8:290549/135‑1 (MQ=255)
                                                    gcATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGCGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCa                                                                                >  3:93205/1‑135 (MQ=255)
                                                            aaGAGCGACGCCGAGCCGGGCGTCATGGCCGCATTCATCCTCGAGCCAGTAGTAGGCGAAGGCGGTTTCCTGCCAGCGCCGAAAGAGCTGTTGAAGGGCCTGCGCGCGCTGTGAGACAAGCACGGCATCctg                                                                           <  4:307437/132‑1 (MQ=255)
                                                              gAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGAt                                                                      <  1:16997/135‑1 (MQ=255)
                                                                   acgCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCcg                                                                 <  7:391926/135‑1 (MQ=255)
                                                                   acgCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCcg                                                                 <  7:156883/135‑1 (MQ=255)
                                                                             gcgcgACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACa                                                       <  2:316638/135‑1 (MQ=255)
                                                                                  aCATCGCCGCAATCATCCTCGAGCCAGTACATGGCGAAGGCGGCTTCCCGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTTCAAACTg                                                  <  8:121274/135‑1 (MQ=255)
                                                                                  aCATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTg                                                  <  1:157935/135‑1 (MQ=255)
                                                                                  aCATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTg                                                  <  5:117500/135‑1 (MQ=255)
                                                                                  aCATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCCGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTg                                                  <  8:116182/135‑1 (MQ=255)
                                                                                        cctcAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCt                                             <  3:264665/131‑1 (MQ=255)
                                                                                                        gCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACctt                            <  7:313245/135‑1 (MQ=255)
                                                                                                         ccAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACcttc                           >  1:273955/1‑135 (MQ=255)
                                                                                                             tACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCg                       <  8:95718/135‑1 (MQ=255)
                                                                                                             tACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCg                       <  1:22003/135‑1 (MQ=255)
                                                                                                             tACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCg                       <  6:210572/135‑1 (MQ=255)
                                                                                                              aCAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCg                       >  2:47685/1‑134 (MQ=255)
                                                                                                               cAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGcc                     >  8:81925/1‑135 (MQ=255)
                                                                                                                  ggcgaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATg                  <  7:34810/135‑1 (MQ=255)
                                                                                                                  ggcgaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATg                  <  1:126915/135‑1 (MQ=255)
                                                                                                                  ggcgaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATg                  <  5:42344/135‑1 (MQ=255)
                                                                                                                    cgaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATGGa                >  3:253519/1‑135 (MQ=255)
                                                                                                                     gaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGTCCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCg                       <  4:183950/127‑1 (MQ=255)
                                                                                                                     gaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGTCCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCg                       >  3:183950/1‑127 (MQ=255)
                                                                                                                     gaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATGGa                >  5:210444/1‑134 (MQ=255)
                                                                                                                     gaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATGGa                >  7:111005/1‑134 (MQ=255)
                                                                                                                     gaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATGGa                >  2:5538/1‑134 (MQ=255)
                                                                                                                     gaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATGGa                >  8:300637/1‑134 (MQ=255)
                                                                                                                     gaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCGTCGCCATGGa                >  2:149083/1‑134 (MQ=255)
                                                                                                                     gaaggcgGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGATGGCCGTACCCGCACCTTCTTCGCCATGGa                >  8:258061/1‑134 (MQ=255)
                                                                                                                          cggcTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCGCCTTCTTCGCCATGGAACAGAt          >  5:37095/1‑135 (MQ=255)
                                                                                                                          cggcTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCCCCTTCTTCGCCATGGAACAGAt          >  8:29228/1‑135 (MQ=255)
                                                                                                                          cggcTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATGGAACAGAt          >  1:91102/1‑135 (MQ=255)
                                                                                                                          cggcTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATGGAACAGAt          >  5:150382/1‑135 (MQ=255)
                                                                                                                             cTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATGGAACAGATggg       <  4:192548/135‑1 (MQ=255)
                                                                                                                                ccTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCCCCTTCTGCGCCATGGAACAGATGGGCGt    >  4:254998/1‑135 (MQ=255)
                                                                                                                                  tGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATGGAACAGATGGGCGTTg  <  1:47685/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                      
CCGAGCGAACTGCACGGTATCAGCGTTGACGACGCCATCGCCTCGGTCGAGCGCATCTTCAAGAACGACGCCGAGCCGCGCGACATCGCCGCAATCATCCTCGAGCCAGTACAAGGCGAAGGCGGCTTCCTGCCAGCGCCGAAAGAGCTGATGAAGCGCCTGCGCGCCCTGTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAAGTACAAACTGGCGCTGGCCGTACCGGCACCTTCTTCGCCATGGAACAGATGGGCGTTG  >  KT2440/258438‑258702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑