Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	392,749	G→C	7.2%	intergenic (‑116/‑66)	yihG ← / → CJ019_00361	putative acyltransferase YihG/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	392,749	0	G	C	7.2%	207.4 / 5.6	64	intergenic (‑116/‑66)	yihG/CJ019_00361	putative acyltransferase YihG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/33);  new base C (3/2);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGGCGCTGCCGGTGATGGCCGCCGAACCC  >  KT2440/392626‑392874                                                                                                                            |                                                                                                                              cGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGc                                                                                                                    <  6:375018/135‑1 (MQ=255) cGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGc                                                                                                                    <  2:244294/135‑1 (MQ=255)      cATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAAcc                                                                                                               <  5:89233/135‑1 (MQ=255)      cATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAAcc                                                                                                               <  5:39226/135‑1 (MQ=255)      cATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAAcc                                                                                                               <  5:217503/135‑1 (MQ=255)      cATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAAcc                                                                                                               <  2:290037/135‑1 (MQ=255)           agagACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCt                                                                                                          <  3:357976/135‑1 (MQ=255)                ctctTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCa                                                                                                     <  3:380141/135‑1 (MQ=255)                ctctTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCa                                                                                                     <  8:82783/135‑1 (MQ=255)                ctctTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCa                                                                                                     <  6:260755/135‑1 (MQ=255)                ctctTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCa                                                                                                     <  5:346317/135‑1 (MQ=255)                ctctTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCa                                                                                                     <  5:301105/135‑1 (MQ=255)                 tctTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAg                                                                                                    >  5:349807/1‑135 (MQ=255)                  ctTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGt                                                                                                   >  4:148074/1‑135 (MQ=255)                  ctTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGt                                                                                                   >  2:19154/1‑135 (MQ=255)                  ctTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGt                                                                                                   >  7:84113/1‑135 (MQ=255)                    tgttgtGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGt                                                                                                   >  2:266918/1‑133 (MQ=255)                    tgttgtGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGCCTCGTGACAGCGAACCTATCTGCCCAGt                                                                                                   >  2:267137/1‑133 (MQ=255)                       tgtgGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGCCGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGt                                                                                              >  7:184188/1‑135 (MQ=255)                       tgtgGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAg                                                                                               <  1:19154/134‑1 (MQ=255)                       tgtgGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAg                                                                                               <  6:349807/134‑1 (MQ=255)                              gaTAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCa                                                                                       >  2:231325/1‑135 (MQ=255)                              gaTAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCa                                                                                       >  3:276712/1‑135 (MQ=255)                              gaTAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCa                                                                                       >  7:169990/1‑135 (MQ=255)                              gaTAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCa                                                                                       >  2:305261/1‑135 (MQ=255)                                tataCGGTCGGCAATGATAATCAGGGCGGTGGCCTACGCCAAATGTCCAATGCACGACGGCGGGGACAAATGTTTCACATTATGTTTGTTAGGCTCGTGACAGAGAACCTATCTGCCCAGTGCAGTCTAAGCAtt                                                                                     <  8:162841/132‑1 (MQ=255)                                tAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCAtt                                                                                     <  7:294731/135‑1 (MQ=255)                                            aaTGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGCCTCGTGACAGCGAAcc                                                                                                               <  3:210180/97‑1 (MQ=255)                                            aaTGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGCCTCGTGACAGCGAAcc                                                                                                               <  3:214621/97‑1 (MQ=255)                                            aaTGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGCCTCGTGACAGCGAAcc                                                                                                               >  4:214621/1‑97 (MQ=255)                                            aaTGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGCCTCGTGACAGCGAAcc                                                                                                               >  4:210180/1‑97 (MQ=255)                                              tGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGa                                                                       <  2:42655/135‑1 (MQ=255)                                               gATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGaa                                                                      >  2:40090/1‑135 (MQ=255)                                                  aaGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGcc                                                                   <  2:221766/135‑1 (MQ=255)                                                    gCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTACGCATTGACCCTCTCAAGGAAGCCt                                                                  >  8:78064/1‑134 (MQ=255)                                                           ggTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTtgtg                                                           >  3:361146/1‑134 (MQ=255)                                                           ggTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTtgtg                                                           >  1:294606/1‑134 (MQ=255)                                                           ggTGGCCTACGCCAAATGGCCAATGCGCGACGGCTGGGACAAATGTTTCACATTATGTTGGCTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGa                                                          <  1:191624/135‑1 (MQ=255)                                                             tGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGaaa                                                        >  5:27044/1‑135 (MQ=255)                                                                  tACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTAACATTATGTTGGTTAGGCTCGTGCCAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCGCTCAAGGAAGCAGGCTTGTGaaagcgct                                                   >  1:72446/1‑130 (MQ=255)                                                                      ccAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTa                                               <  4:146726/135‑1 (MQ=255)                                                                      ccAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCACTTTTa                                               >  7:386124/1‑135 (MQ=255)                                                                            gTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCAtg                                         >  1:156106/1‑135 (MQ=255)                                                                             tCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCAtt                                                                                     <  1:368361/90‑1 (MQ=255)                                                                             tCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCAtt                                                                                     >  2:368361/1‑90 (MQ=255)                                                                                  tGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCg                                   >  3:328536/1‑135 (MQ=255)                                                                                          ggcggGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGgc                           >  4:57880/1‑135 (MQ=255)                                                                                           gcggGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGgcg                          <  3:148074/135‑1 (MQ=255)                                                                                                           cacaTTATGTTGGGTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGGCGCTGCCGGTGATGgcc           <  1:305261/134‑1 (MQ=255)                                                                                                            acaTTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCa                                                                                                     >  1:57321/1‑43 (MQ=255)                                                                                                            acaTTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCa                                                                                                     >  1:63462/1‑43 (MQ=255)                                                                                                            acaTTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCa                                                                                                     <  2:57321/43‑1 (MQ=255)                                                                                                            acaTTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCa                                                                                                     <  2:63462/43‑1 (MQ=255)                                                                                                             caTTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCa                                                                                                     >  5:82605/1‑42 (MQ=255)                                                                                                             caTTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCa                                                                                                     <  6:82605/42‑1 (MQ=255)                                                                                                             caTTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGGCGCTGCCGGTGATGgccgcc        >  2:346318/1‑135 (MQ=255)                                                                                                             caTTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGGCGCTGCCGGTGATGgccgc         <  2:294606/134‑1 (MQ=255)                                                                                                              aTTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGGCGCTGCCGGTGATGGccgccg       <  6:29415/135‑1 (MQ=255)                                                                                                                  tGTTGGTTAGGCTCGTGACATCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGGCGCTGCCGGTGATGGCCGCCGAAc    <  5:225913/134‑1 (MQ=255)                                                                                                                  tGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCACGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATTTTGTCGCTGTTGGCGCTGCCGGTGATGGCCGCCGAAc    <  4:243154/134‑1 (MQ=255)                                                                                                                  tGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGGCGCTGCCGGTGATGGCCGCCGAAc    <  1:266918/134‑1 (MQ=255)                                                                                                                  tGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGGCGCTGCCGGTGATGGCCGCCGAAc    <  6:258495/134‑1 (MQ=255)                                                                                                                  tGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGGCGCTGCCGGTGATGGCCGCCGAAc    <  4:263058/134‑1 (MQ=255)                                                                                                                   gTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGGCGCTGCAGGTGATGGCCGCCGAAccc  >  6:365770/1‑135 (MQ=255)                                                                                                                            |                                                                                                                              CGACGCATAAAGAGACTCTTGTTGTGGGAGATAAACGGTTGGCAATGATAAGCAGGGCGGTGGCCTACGCCAAATGTCCAATGCCCGACGGCGGGGACAAATGTTTCACATTATGTTGGTTAGGCTCGTGACAGCGAACCTATCTGCCCAGTGCAGTCTAAGCATTGACCCTCTCAAGGAAGCCTGCTTGTGAAATCTCTTTTAGCCATGTTGTCGCTGTTGGCGCTGCCGGTGATGGCCGCCGAACCC  >  KT2440/392626‑392874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑