Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	395,017	A→C	16.6%	L187V (TTG→GTG)	yibF ←	putative GST‑like protein YibF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	395,017	0	A	C	16.6%	72.3 / 3.1	47	L187V (TTG→GTG)	yibF	putative GST‑like protein YibF
Reads supporting (aligned to +/- strand):  ref base A (28/10);  new base C (2/7);  total (30/17)
Fisher's exact test for biased strand distribution p-value = 6.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTC  >  KT2440/394888‑395149                                                                                                                                   |                                                                                                                                     cccACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGCCCATCGATGGCCGCTTGACCACCACGGCATACCACGCCGCCAACCCAg                                                                                                                                 >  5:145722/1‑135 (MQ=255)       acaGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCCACCCCGGCTGgc                                                                                                                           >  4:186149/1‑135 (MQ=255)       acaGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCCGGCTGgc                                                                                                                           >  3:368707/1‑135 (MQ=255)       acaGATAGCGAACACGGTGGAAAACCTTGTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCAGCGATGGCCGCATGACCCCCTCGGCATACCACGCCGCCCACCCAGGCTGgc                                                                                                                           >  2:264713/1‑135 (MQ=39)           aTAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTc                                                                                                                       <  8:229438/135‑1 (MQ=255)           aTAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGGTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTc                                                                                                                       <  7:27506/135‑1 (MQ=255)                gAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCCCTTGACCACCACGGCCTACCACCCCGCCAACCCAGCCTGGCGCGCACgcc                                                                                                                  >  7:236418/1‑135 (MQ=39)                  acacTGTGGAAAACCTTGTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCACGCTGGCGCTCACgccag                                                                                                                >  8:93612/1‑135 (MQ=255)                      tgtgGAAAACCTTGTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCAACCAAGGCCCCGTGACCACCTCCGCATACCCCGCCGCCACCCAAGCCTGGCGCACACGCCCCCCaa                                                                                                            >  4:210272/1‑135 (MQ=37)                           aaaaCCTTGTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCg                                                                                                       >  4:337616/1‑135 (MQ=255)                                tttGTCGGCGGGGGTTTCCCG‑GCTAAGGGGCCCGCAGGCCACTGGCGCTGTAGCGACCATCGAGGGCCGCTTGACCACCTCGGCAAACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTg                                                                                                  <  3:186149/134‑1 (MQ=255)                                   gTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGCCCATCGATGGCCGCTTGACCACCTCGGCCTACCACGCCGCCACCCCCGGCTGGCGCTCACGCCCGCCAAACCCGGGCTGCCg                                                                                               >  3:177727/1‑135 (MQ=255)                                   gTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCACGCTGGCGCTCACGCCAGCCAAACCCGGGCTGAc                                                                                                >  4:353173/1‑134 (MQ=255)                                      ggAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCa                                                                                             <  6:67257/134‑1 (MQ=255)                                           ggggTTACCCGGCGATGGGGCCCTCAGCCCATGGGCGCGGTCGCCCCAATCTAGGGCCGCTTGACCACCTCGGAAACCCCCGCCGCCACCCCCGGCGGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATcc                                                                                        <  6:204031/135‑1 (MQ=255)                                            ggTTTACCC‑GCGAAGGGGCCCTCCGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGCCCACCTCGGCAAACCACGCCGCCCAACCACGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAg                                                                                      >  8:223537/1‑135 (MQ=255)                                            ggTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCCCGCCGCCAACCCAGGCAGGCGCTCCCGCCACCCCCACCCCCGCGGACGCaaaaaca                                                                                       >  3:209587/1‑130 (MQ=255)                                             gTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATAGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCCCGCTGGCGCTCACGCCAGCCCAACCCGGGCGGACGCAAAACCAg                                                                                      >  7:238877/1‑134 (MQ=255)                                                 aCCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCCCGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGc                                                                                 >  6:122452/1‑135 (MQ=255)                                                      gggatggggCCCTCCGGCCACTGGCGCTTTAGCGACATTCGAGGGCCGTTTGACCACCTCGCCATACCCCCCCGCAACCCCCGGCGGGCGCACACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAg                                                                             <  4:226076/130‑1 (MQ=255)                                                       cgATGGGGCCCGCCGGCAACGGGGGGTGTCGCCACCATCGAGGGCCGCTTCCCCACGCCGCAATACCACGCCGCCAACCCCGGCGGGCGCTCCCCCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAgc                                                                            <  4:209587/135‑1 (MQ=255)                                                       cgACGGGCCCCTCCGGCCCCGGGCGCTGTCGCCACCAGCGAGGCCCGCTTCACCCCCGCGCCAACCCCCCCCGCCACCCCCGGCGGGCGCTCCCCCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAgc                                                                            <  1:22394/135‑1 (MQ=255)                                                           ggggCCCTCAGGCCACTGGCGCTGCAGCGCCCATCGATGGCCGCTTGCCCACCACGGCATACCCCGCCGCCCACCCCCGCGGGCGCGCACGCCCGCCCCACCCGGGCGGCCGCCAAAGCAGAAAGCCCAGCGcac                                                                        >  1:161927/1‑135 (MQ=255)                                                             ggCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCCGGCGGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGcaca                                                                       <  4:295066/134‑1 (MQ=255)                                                                 cTCAGGCCACTGGCGCTGTAGCGACCATCGAGGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCGGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCa                                                                  <  8:60629/135‑1 (MQ=255)                                                                              cgcTGTAGCGACCATCGATGGCCGCTTGACCCCCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCCCGCCCGCCCAACCCGGGCTGCCGCAAATCCAGATAGCCCCGCGCCCAGGCCCCGCCAATcgccgc                                                     >  1:283444/1‑135 (MQ=255)                                                                                   tAGCGCCCATCGATGGCCGCTTGACCCCCTCGGCCTACCACGCCGCCAACCCCGGCTGGCGCTCACGCCAGCCAAACCCGGGCGGACGCAACTCCAGCTAGCCCCGCGCACAGGCCCCGCCCATCGCCGCCAGGt                                                >  3:365175/1‑135 (MQ=255)                                                                                   acgCGACCAGCGATGGCGGCTTCACCACCACGGCTACCCACGCCGCCACCCCCGGCGGGCGCTCACGCCCCCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGt                                                <  1:234370/133‑1 (MQ=255)                                                                                      cGAGCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCCGGCTGGCGCTCCCGCCCGCCACACCCGGGCTGTCGCAAATCCAGATAGCCCAGCGCCCACGCCACGCCAAACGCCGCCAGGTCGa                                             >  3:326026/1‑135 (MQ=255)                                                                                          cATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCCGCGCACCGGCCACGCCAATCGCCGCCAGGTCgcaccc                                         >  6:221624/1‑130 (MQ=255)                                                                                           aTCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATATCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCg                                        >  2:237993/1‑135 (MQ=255)                                                                                           aTCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGcaca                                                                       <  8:83257/104‑1 (MQ=255)                                                                                           aTCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGcaca                                                                       >  7:83257/1‑104 (MQ=255)                                                                                           aTCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCg                                        >  5:87512/1‑135 (MQ=255)                                                                                              gaggcccGCTTCCCCACCTCGCCAAACCCCCCCCCCACCCCCGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGga                                      <  4:68827/129‑1 (MQ=255)                                                                                                tGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGgaga                                    >  6:66043/1‑134 (MQ=255)                                                                                                  gCCGCTTCACCCCCCCGCCATCCCCCGCCCCCACCCCCGGGGGGCGCGCCCCCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCa                                 <  7:66588/135‑1 (MQ=255)                                                                                                         gACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCCCGCCCGCCAAACCCGGGCGGCCGCCAATCCAGATAGCCCAGCGCCCAGGCCACGCCAAACGCCGCCACGTCCAAGCCGGAGAACAGcgacgg                          >  1:195781/1‑130 (MQ=255)                                                                                                         gACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATAAGCTCCg                           >  6:138808/1‑134 (MQ=255)                                                                                                               cTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtg                    <  4:111296/135‑1 (MQ=255)                                                                                                               cTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtg                    >  5:89893/1‑135 (MQ=255)                                                                                                               cTCGCCATCCCACCCCGCCACCCCCGGCTGCCCCCCACGCCACCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtg                    <  3:337616/135‑1 (MQ=255)                                                                                                                  ggCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAAACGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGtgctc                 >  3:278384/1‑135 (MQ=255)                                                                                                                              cgccAACCCAGGCTGGCGCTCACCCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGtt     <  2:24954/135‑1 (MQ=255)                                                                                                                               gccAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTg    >  7:238721/1‑135 (MQ=255)                                                                                                                                ccAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGt   >  8:237855/1‑135 (MQ=255)                                                                                                                                 cAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCCGGTCGAATCCGGCGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTc  >  6:353196/1‑135 (MQ=255)                                                                                                                                   |                                                                                                                                     CCCACCACAGATAGCGAACACTGTGGAAAACCTTGTAGGAGCGGGTTTACCC‑GCGAAGGGGCCCTCAGGCCACTGGCGCTGTAGCGACCATCGATGGCCGCTTGACCACCTCGGCATACCACGCCGCCAACCCAGGCTGGCGCTCACGCCAGCCAAACCCGGGCTGACGCAAATCCAGATAGCCCAGCGCACAGGCCACGCCAATCGCCGCCAGGTCGAAGCCGGAGATCAGCTCCGGCAAGTGCTCCTGCTCCAGGTTGTC  >  KT2440/394888‑395149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑