Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	91,899	T→G	17.6%	L96V (TTG→GTG)	prlC →	Oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	91,899	0	T	G	17.6%	86.7 / 2.0	43	L96V (TTG→GTG)	prlC	Oligopeptidase A
Reads supporting (aligned to +/- strand):  ref base T (4/30);  new base G (7/2);  total (11/32)
Fisher's exact test for biased strand distribution p-value = 3.00e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGGCCTGGTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCGACGTGGCGCAAAAGACTATCCTCGAGCACGCCTTGCGTGACTTC  >  KT2440/91770‑92033                                                                                                                                  |                                                                                                                                       gccggccgGGTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAgc                                                                                                                                   <  5:289722/135‑1 (MQ=255)   cggccTGGTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAgcgc                                                                                                                                 <  5:165216/135‑1 (MQ=255)   cggccTGGTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAgcgc                                                                                                                                 <  8:251100/135‑1 (MQ=255)   cggccTGGTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAgcgc                                                                                                                                 <  8:116629/135‑1 (MQ=255)    gggcTGGTGCTGGCCATGGACTAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCGGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGcc                                                                                                                                <  7:211187/132‑1 (MQ=255)    ggccTGGTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGGCGGCCGGCGGTGAgcgg                                                                                                                                 >  8:373053/1‑133 (MQ=255)     gccTGGTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGGCGGCCGGCGGTGAGCGccg                                                                                                                               >  7:300836/1‑134 (MQ=255)     gccTGGTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCt                                                                                                                               >  4:111925/1‑135 (MQ=255)     gccTGGTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCt                                                                                                                               <  3:230710/135‑1 (MQ=255)       cTGGTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTAc                                                                                                                             >  5:195688/1‑135 (MQ=255)        tGGTGCTGGCCATGGGCGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGGCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGGCAGCCGGCGGTGCGCGCCCAcg                                                                                                                            >  2:289650/1‑134 (MQ=255)         ggTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTActc                                                                                                                           <  5:65802/135‑1 (MQ=255)              tGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTGCCg                                                                                                                      <  7:132651/135‑1 (MQ=255)              tGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCg                                                                                                                      <  6:42029/135‑1 (MQ=255)              tGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCg                                                                                                                      <  5:265903/135‑1 (MQ=255)              tGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCg                                                                                                                      <  6:249180/135‑1 (MQ=255)              tGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCg                                                                                                                      <  1:12287/135‑1 (MQ=255)              tGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCAGCCCGCCTTGCGCGCCGACACAAcc                                                                                                                       >  7:282469/1‑134 (MQ=255)                  cATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGGGCCTAATCTACCGCACt                                                                                                                  >  7:233662/1‑135 (MQ=255)                   aTGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTg                                                                                                                 <  6:195688/135‑1 (MQ=255)                   aTGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCGGCCGGCCGTTCGCGCCGACTCTACCGCACTg                                                                                                                 >  7:26650/1‑135 (MQ=255)                        cGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCGGCCCGCGGTGCGCGCCGTCTCTACCGCACTGGGccc                                                                                                            >  6:364294/1‑134 (MQ=255)                              gAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGGCTGCCGGCGGTGAGCGCCGACTCCACCCAACAGGGCCCGAAccc                                                                                                      >  4:4640/1‑134 (MQ=255)                               caCGACCGCCTGGGCGCCGCCTGGAGCCCGGGCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGGGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACcgc                                                                                                     <  1:289650/134‑1 (MQ=255)                                aCGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACcgc                                                                                                     <  2:18830/134‑1 (MQ=255)                                      gCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCACTGCGGTGTGCAACAGGGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGt                                                                                              <  5:257971/135‑1 (MQ=255)                                                  ccTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTAcc                                                                                  <  3:68235/135‑1 (MQ=255)                                                    tGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTACCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAg                                                                                <  4:388690/135‑1 (MQ=255)                                                            ggTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGAt                                                                        <  3:61333/135‑1 (MQ=255)                                                            ggTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGAt                                                                        <  3:4640/135‑1 (MQ=255)                                                            ggTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGAt                                                                        <  7:373053/135‑1 (MQ=255)                                                                         aaTGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCGGCCCGGGGTGGGCCGCGACGCGACCGCACAGGGCCCGCACCCCCCGCGGGTAGACGGCCACCCGGCGCGGGTCGCCAGcccggcg                                                           >  2:104797/1‑131 (MQ=255)                                                                                      aCAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCg                                              <  3:124452/135‑1 (MQ=255)                                                                                             cGAGTTGCGTGAGGCCTAAGAGTCGGGCCTTCCGGCGGTGCGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGACTAGCAGGCGCTGATCGACAGCCCTGATGCTGCCGGTTTCGACGTGgc                                       <  6:216820/135‑1 (MQ=255)                                                                                                  tGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCGACGTGGCGCaaa                                  <  2:375504/135‑1 (MQ=255)                                                                                                    cGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAATCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCGACGTGGCGCaaaa                                 <  4:21189/134‑1 (MQ=255)                                                                                                        aGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCGACGTGGCGCAAAAGACTa                            <  1:198764/135‑1 (MQ=255)                                                                                                         ggCCTACGAGTCGGGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCGACGTGGCGCAAAAGACTAt                           <  6:58578/135‑1 (MQ=255)                                                                                                             tACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCGACGTGGCGCAAAAGACTATCCTc                       <  1:268059/135‑1 (MQ=255)                                                                                                             tACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCGACGTGGCGCAAAAGACTATCCTc                       <  8:73321/135‑1 (MQ=255)                                                                                                                   tCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCGACGTGGCGCAAAAGACTATCCTCGAGCAc                 <  2:84664/135‑1 (MQ=255)                                                                                                                        cctgccGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGATCGAAGCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCTGTTTCGACGTGGCTCAAAAGACTATCCTCGAGCACGCCtt            <  1:248098/135‑1 (MQ=255)                                                                                                                        cctgccGGCGGTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCGACGTGGCGCAAAAGACTATCCTCGAGCACGCCtt            <  1:270901/135‑1 (MQ=255)                                                                                                                                  ttGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTATCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCGACGTGGCGCAAAAGACTATCCTCGAGCACGCCTTGCGTGACTTc  <  1:234728/135‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       GCCGGCCTGGTGCTGGCCATGGACGAACTGAACGACCGCCTGGGCGCCGCCTGGAGCCCGGTCAGCCACCTCAATGCGGTGTGCAACAGTGCCGAGTTGCGTGAGGCCTACGAGTCGTGCCTGCCGGCGTTGAGCGCCTACTCTACCGAACTGGGCCAGAACCGCGCGCTGTTCGAAGCCTACCAGGCGCTGATCGACAGCCCTGAGGCTGCCGGTTTCGACGTGGCGCAAAAGACTATCCTCGAGCACGCCTTGCGTGACTTC  >  KT2440/91770‑92033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑