Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 420,269 T→A 6.9% S194C (AGC→TGC)  fhuA_2 ← Ferrichrome outer membrane transporter/phagereceptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440420,2690TA 6.9% 181.2 / 3.2 58S194C (AGC→TGC) fhuA_2Ferrichrome outer membrane transporter/phagereceptor
Reads supporting (aligned to +/- strand):  ref base T (30/24);  new base A (2/2);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

AGCAGGGCGAGGCGGTCGAGGTTCCAGGTTTCGGCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTC  >  KT2440/420136‑420398
                                                                                                                                     |                                                                                                                                 
agcagGGCGAGGCGGTCGAGGTTCCAGGTTTCGGCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTg                                                                                                                                  >  8:327161/1‑135 (MQ=255)
         aGGCGGTCGAGGTTCCAGGTTGCGGCTTGCGGGTTGGCATCCACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTGTCGCTGCCGAAGCTg                                                                                                                         <  3:364272/135‑1 (MQ=255)
                    gTTCCAGGTTTCGGCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGAt                                                                                                              <  4:128573/135‑1 (MQ=255)
                    gTTCCAGGTTTCGGCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGAt                                                                                                              <  8:42283/135‑1 (MQ=255)
                      tCCAGGTTTCGGCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGc                                                                                                            <  8:217794/135‑1 (MQ=255)
                      tCCAGGTTGCGGCTTTCGGTTGGGCATACACGCCGCGGGGCAGTGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGAGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGc                                                                                                            <  5:311112/135‑1 (MQ=255)
                       ccAGGTTTCGGCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGCAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGcc                                                                                                           >  5:117538/1‑135 (MQ=255)
                         aGGTTTCGGCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGccc                                                                                                          <  2:56852/134‑1 (MQ=255)
                            tttCGGCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGc                                                                                                       >  2:369060/1‑134 (MQ=255)
                             gtCGGCTTGCGGGGTGGAATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCat                                                                                                     <  6:333132/134‑1 (MQ=255)
                                 gCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAg                                                                                                 >  3:262359/1‑135 (MQ=255)
                                 gCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCAGCCGAAGCTGGCAGAGACGATGCCCGGCATATAg                                                                                                 >  3:193620/1‑135 (MQ=255)
                                 gCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCAGCCGAAGCTGGCAGAGACGATGCCCGGCATATAg                                                                                                 >  3:200098/1‑135 (MQ=255)
                                  cTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAgc                                                                                                >  6:360012/1‑135 (MQ=255)
                                  cTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAgc                                                                                                <  3:119121/135‑1 (MQ=255)
                                      cGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCAc                                                                                            <  6:377102/135‑1 (MQ=255)
                                       gggTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCAc                                                                                            <  3:371702/134‑1 (MQ=255)
                                       gggTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCAc                                                                                            <  4:280835/134‑1 (MQ=255)
                                       gggTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCAGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCAc                                                                                            <  4:193620/134‑1 (MQ=255)
                                       gggTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCAGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCAc                                                                                            <  4:200098/134‑1 (MQ=255)
                                        ggTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACt                                                                                           >  2:178705/1‑134 (MQ=255)
                                             gCATACACGCCGCGGGGCAGTGGTAGTCCAGCGAGGAACTGCGGTGGCTCGAATCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGTCGATTCCCGGCATATAGCGCACTGcatca                                                                                     <  6:57401/135‑1 (MQ=255)
                                             gCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGGCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGcatca                                                                                     <  8:176123/135‑1 (MQ=255)
                                              cATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGcatca                                                                                     <  1:3069/134‑1 (MQ=255)
                                              cATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGcatca                                                                                     <  6:117538/134‑1 (MQ=255)
                                               aTACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGcatcat                                                                                    >  1:149503/1‑134 (MQ=255)
                                                tACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATcc                                                                                  <  1:26295/135‑1 (MQ=255)
                                                tACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATcc                                                                                  <  3:18245/135‑1 (MQ=255)
                                                tACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATcc                                                                                  <  5:247834/135‑1 (MQ=255)
                                                tACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATcc                                                                                  <  5:69329/135‑1 (MQ=255)
                                                          ggggCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCTCGTATCGCTGACGAAGCTGGCAGAGACGATGACCGGCATATAGCGCACTGCATCATCCAGGATgtgt                                                                         >  3:320613/1‑134 (MQ=255)
                                                          ggggCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTgtgt                                                                         >  4:20246/1‑134 (MQ=255)
                                                          ggggCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTgtgt                                                                         >  4:321173/1‑134 (MQ=255)
                                                          ggggCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTa                                                                        >  5:159024/1‑135 (MQ=255)
                                                            gggaGGGGTAGGGCATCTAGGCACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCAGGCATATAGCGCACTGCATCATCCAGGCTGTGTaca                                                                      <  1:351173/132‑1 (MQ=255)
                                                                  ggTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACcgcg                                                                 <  4:303996/134‑1 (MQ=255)
                                                                         cATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGcatca                                                                                     <  1:250821/107‑1 (MQ=255)
                                                                         cATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGcatca                                                                                     >  2:250821/1‑107 (MQ=255)
                                                                         cATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCa                                                                                 >  5:261892/1‑111 (MQ=255)
                                                                         cATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCa                                                                                 <  6:261892/111‑1 (MQ=255)
                                                                         cATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCa                                                         >  6:97932/1‑135 (MQ=255)
                                                                              aGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCCTCCAGGCTGTGTACACCGCGGTCTTTCATTTGc                                                    >  4:67881/1‑135 (MQ=255)
                                                                               ggAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCt                                                   >  7:344613/1‑135 (MQ=255)
                                                                                         tGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGc                                         >  3:119084/1‑135 (MQ=255)
                                                                                           gCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCGTTCAGTTGCTCGCGGGTGGCGa                                       >  5:27741/1‑135 (MQ=255)
                                                                                              cGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGAc                                      >  3:80696/1‑133 (MQ=255)
                                                                                                   ccGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCa                                                                                 >  5:2959/1‑85 (MQ=255)
                                                                                                   ccGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCa                                                                                 <  6:2959/85‑1 (MQ=255)
                                                                                                         acgcgcaTCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGGCAGAGCCGGAGCgg                         >  5:291003/1‑135 (MQ=255)
                                                                                                                   aGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCGAGGCTGTGTACACCGCGGTCATTCATTTGCTCGCGGGGGGCGACAGACACGCCGCGGGGGACTTcc                >  8:202828/1‑134 (MQ=255)
                                                                                                                            gcgTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCg      >  4:277272/1‑135 (MQ=255)
                                                                                                                            gcgTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCg      >  3:291260/1‑135 (MQ=255)
                                                                                                                            gcgTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCg      >  6:141404/1‑135 (MQ=255)
                                                                                                                            gcgTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGGGGCGCCAGCGCCGGAGCGGGGGACTTCCACCAGTGCCg      >  8:384538/1‑135 (MQ=255)
                                                                                                                              gTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCgtg    >  7:42056/1‑135 (MQ=255)
                                                                                                                               tATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCgtg    >  8:168890/1‑134 (MQ=255)
                                                                                                                                aTCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCgtggc  >  5:222221/1‑133 (MQ=255)
                                                                                                                                aTCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCgtg    >  3:170685/1‑133 (MQ=255)
                                                                                                                                     |                                                                                                                                 
AGCAGGGCGAGGCGGTCGAGGTTCCAGGTTTCGGCTTTCGGGTTGGCATACACGCCGCGGGGCAGGGGTAGGCCATCGAGGAACTGCGTTGGCTCGAAGCCGCGCACGCGCATCCAGTCATACCGCGTATCGCTGCCGAAGCTGGCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTC  >  KT2440/420136‑420398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑