| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 545,486 | T→A | 5.9% | V46D (GTC→GAC) | rplW → | 50S ribosomal protein L23 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 545,486 | 0 | T | A | 5.9% | 202.1 / 3.9 | 68 | V46D (GTC→GAC) | rplW | 50S ribosomal protein L23 |
| Reads supporting (aligned to +/- strand): ref base T (28/36); new base A (2/2); total (30/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01 | |||||||||||
CAGGAACGCGTATTCAAAGTCCTCCTTGGCCCGCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGCGTAATGACTGGAAGAAGGCGATCGTCTCCCTTCAGCCAG > KT2440/545356‑545614 | cAGGAACGCGTATTCAAAGTCCTCCTTGGCCCGCTCGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGaa < 4:341744/135‑1 (MQ=255)cAGGAACGCGTATTCAAAGTCCTCCTTGGCCCGCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGTCCAGTTAGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGaa > 6:153979/1‑135 (MQ=255)cAGGAACGCGTATTCAAAGTCCTCCTTGGCCCGCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCACCCAAGCTGGAAATCAAGAAAGCTGTCGaa > 4:62549/1‑135 (MQ=255) aTTCAAAGTCCTCCTTGGCCCGCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCaa > 6:141201/1‑135 (MQ=255) aaaGTCCTCCTTGGCCCGCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGt < 5:204770/134‑1 (MQ=255) tcctTGGCCCGCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGtt < 3:278386/134‑1 (MQ=255) cccGCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACgt < 5:153979/135‑1 (MQ=255) gCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACgtgt > 6:191735/1‑134 (MQ=255) gCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACgtgt > 1:280295/1‑134 (MQ=255) gCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTc < 3:22300/135‑1 (MQ=255) gCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTc < 8:346158/135‑1 (MQ=255) gCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTc > 5:376870/1‑135 (MQ=255) gTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACt < 3:81501/135‑1 (MQ=255) gTTTCCGAGAAGGCTACCGTTCAGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACCTAAAAGTTGAAAACGTGTCGACt > 5:319433/1‑135 (MQ=255) tttCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTg > 7:68707/1‑135 (MQ=255) gagaAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAAc < 5:191735/135‑1 (MQ=255) gagaAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAAc < 8:40913/135‑1 (MQ=255) gagaAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAAc < 2:93487/135‑1 (MQ=255) gagaAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAAc < 1:148554/135‑1 (MQ=255) gagaAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAAc < 1:137068/135‑1 (MQ=255) gagaAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAAc < 1:93216/135‑1 (MQ=255) cTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTgg < 3:64972/135‑1 (MQ=255) aCCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGt > 1:200299/1‑98 (MQ=255) aCCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGt < 2:200299/98‑1 (MQ=255) aCCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTTTTAACGTTCTggg > 4:260039/1‑134 (MQ=255) aCCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTgtg > 4:157330/1‑132 (MQ=255) cGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGt < 7:69941/133‑1 (MQ=255) cGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACCTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTa > 4:302497/1‑134 (MQ=255) tGAGAAAAAAGGCCAGTTCGTATTCAAGGGTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCg < 4:89558/135‑1 (MQ=255) gagaAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGt > 7:110442/1‑135 (MQ=255) aaaaGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCATGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGc < 6:219990/135‑1 (MQ=255) aaaaGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGc > 2:252900/1‑135 (MQ=255) aaaaGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGc < 1:143125/135‑1 (MQ=255) aaaaGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGc < 8:183202/135‑1 (MQ=255) aaaaGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGc > 6:248107/1‑135 (MQ=255) aaaaGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTGCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGc < 6:194683/135‑1 (MQ=255) tgCCAGTTCGTATTCAAGGTTTCTACTGATGCAACGAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTTTTAACGTTCTGGGTAAAACCACGCGTACCGCACGt < 4:286321/134‑1 (MQ=255) ggCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGt < 5:141201/135‑1 (MQ=255) ggCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGt < 3:298772/135‑1 (MQ=255) ggCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGATCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGt < 3:322992/135‑1 (MQ=255) gCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCTACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTg > 1:131643/1‑135 (MQ=255) gCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTg > 1:77046/1‑135 (MQ=255) ttCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTg < 3:157330/135‑1 (MQ=255) cAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCg < 7:103239/116‑1 (MQ=255) cAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCg > 8:103239/1‑116 (MQ=255) aGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGCGt < 7:61188/134‑1 (MQ=255) ggTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGCGTaa < 3:56261/135‑1 (MQ=255) gTTTCTACTGATGCATCCAAGCTGTAATTCAAGATAGCTGTGGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGCGTAAt < 1:339075/135‑1 (MQ=255) ttGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGCGTaacg > 3:290041/1‑133 (MQ=255) tGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGCGTAATGa > 8:107975/1‑135 (MQ=255) aCTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTg < 1:121424/76‑1 (MQ=255) aCTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTg > 2:121424/1‑76 (MQ=255) gCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGCGTAATGACTGGaagaag > 1:274665/1‑135 (MQ=255) cAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTaaa < 4:37043/86‑1 (MQ=255) cAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTaaa > 3:37043/1‑86 (MQ=255) cAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGc > 7:365833/1‑117 (MQ=255) cAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGc < 8:365833/117‑1 (MQ=255) cAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGATAACGTGACGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGCGTAATGACTGGAAGAAGGCGAt < 2:104308/135‑1 (MQ=255) aaGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCa > 7:256696/1‑85 (MQ=255) aaGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCa < 8:256696/85‑1 (MQ=255) aaGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGCGTAATGACTGGAAGAAGGCGATc > 8:91583/1‑135 (MQ=255) aaGCTGGAAATCAAGAAAGCTGACGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTaa > 7:236692/1‑80 (MQ=255) aaGCTGGAAATCAAGAAAGCTGACGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTaa < 8:230620/80‑1 (MQ=255) aaGCTGGAAATCAAGAAAGCTGACGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTaa < 8:236692/80‑1 (MQ=255) aaGCTGGAAATCAAGAAAGCTGACGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTaa > 7:230620/1‑80 (MQ=255) ggAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGc < 5:380100/90‑1 (MQ=255) ggAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGc > 6:380100/1‑90 (MQ=255) aaGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGCGTAATGACTGGAAGAAGGCGATCGTCTCCCTTCAGCCAg > 3:215023/1‑135 (MQ=255) | CAGGAACGCGTATTCAAAGTCCTCCTTGGCCCGCACGTTTCCGAGAAGGCTACCGTTCTGGCTGAGAAAAAAGGCCAGTTCGTATTCAAGGTTGCTACTGATGCAACCAAGCTGGAAATCAAGAAAGCTGTCGAAGGCCTGTTCAACGTAAAAGTTGAAAACGTGTCGACTGTTAACGTTCTGGGTAAAACCAAGCGTACCGCACGTGGTCTGGGCAAGCGTAATGACTGGAAGAAGGCGATCGTCTCCCTTCAGCCAG > KT2440/545356‑545614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |