Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 709,887 G→A 7.5% G139S (GGC→AGC)  CJ019_00664 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440709,8870GA 7.5% 157.7 / 3.0 53G139S (GGC→AGC) CJ019_00664hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/22);  new base A (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

AGAACCTGGGTGTCAGCGAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGCCTACCCCAGTT  >  KT2440/709759‑710020
                                                                                                                                |                                                                                                                                     
aGAACCTGGGTGTCAGCGAACGTGCCGCGCACATGCCCGACGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCaa                                                                                                                                   <  6:204233/133‑1 (MQ=255)
   aCCTGGGTGTCAGCGAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCaaa                                                                                                                              <  6:113567/135‑1 (MQ=255)
          tgtCATCGAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGACTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGTAAGCGGTCTACGCCATGGCCGGCAATCCAACCCAACg                                                                                                                       >  4:106272/1‑135 (MQ=255)
          tgtCAGCGAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAAc                                                                                                                        >  6:24418/1‑134 (MQ=255)
          tgtCAGCGAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACg                                                                                                                       >  6:107184/1‑135 (MQ=255)
          tgtCAGCGAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCAGCAATCAAACCCAAc                                                                                                                        >  2:240644/1‑134 (MQ=255)
             cAGCGAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCAt                                                                                                                    <  4:160248/135‑1 (MQ=255)
                 gAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCAcc                                                                                                                >  5:178429/1‑135 (MQ=255)
                 gAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCAcc                                                                                                                >  7:22199/1‑135 (MQ=255)
                 gAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCAcc                                                                                                                >  5:33760/1‑135 (MQ=255)
                 gAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCAcc                                                                                                                >  5:356077/1‑135 (MQ=255)
                 gAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCAGCAATCAAACCCAACGCATCAcc                                                                                                                >  5:345087/1‑135 (MQ=255)
                       gCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCg                                                                                                           >  8:174455/1‑134 (MQ=255)
                       gCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCAGCAATCAAACCCAACGCATCACCTGGCGa                                                                                                          <  3:82669/135‑1 (MQ=255)
                                aTGCCCGAAGGGCAGCCGGTTATCCTGTTCCGGATTACCTCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCACACCCAACGCATCACCTGGCGACAAAGATTg                                                                                                 <  8:220739/135‑1 (MQ=255)
                                 tGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGc                                                                                                <  4:162893/135‑1 (MQ=255)
                                     cGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCGCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCgagg                                                                                            <  7:316681/135‑1 (MQ=255)
                                           gCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTg                                                                                      >  5:172904/1‑135 (MQ=255)
                                             aGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGAc                                                                                    >  7:81549/1‑135 (MQ=255)
                                               ccGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACAt                                                                                  >  6:166483/1‑135 (MQ=255)
                                               ccGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACAt                                                                                  >  5:315536/1‑135 (MQ=255)
                                                 ggTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACAt                                                                                  >  6:218808/1‑133 (MQ=255)
                                                 ggTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACAt                                                                                  >  1:254195/1‑133 (MQ=255)
                                                 ggTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATgc                                                                                <  7:151193/135‑1 (MQ=255)
                                                  gTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATgca                                                                               <  8:81549/135‑1 (MQ=255)
                                                       ccTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAg                                                                          <  2:20098/135‑1 (MQ=255)
                                                       ccTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAg                                                                          <  2:27088/135‑1 (MQ=255)
                                                        cTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAgg                                                                         <  1:383334/135‑1 (MQ=255)
                                                              cGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAg                                                                   >  2:139347/1‑135 (MQ=255)
                                                              cGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAg                                                                   >  5:118338/1‑135 (MQ=255)
                                                                  ttACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCAGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTg                                                               <  6:345087/135‑1 (MQ=255)
                                                                            cAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAgttgt                                                     <  3:96581/135‑1 (MQ=255)
                                                                                              aCGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGa                                   >  3:334377/1‑135 (MQ=255)
                                                                                                       gCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTc                          <  7:174455/135‑1 (MQ=255)
                                                                                                       gCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTc                          <  6:33760/135‑1 (MQ=255)
                                                                                                         tGGAAGCGGTCTACGCCATGGCCGGCAATCACACCCAACGCATCCCCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTGGAGCCTGATCGAGTTGTTGACTGTGCTGGCCGTGCCCGGCATGCTg                        >  6:283724/1‑135 (MQ=255)
                                                                                                             aGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAg                    >  2:255578/1‑135 (MQ=255)
                                                                                                                 gTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCAt                <  3:14208/135‑1 (MQ=255)
                                                                                                                 gTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCAt                <  6:118338/135‑1 (MQ=255)
                                                                                                                  tCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCTAtt               <  6:315536/135‑1 (MQ=255)
                                                                                                                    tACGCCATGGCCGGCAATCAAACCCAACGCATCCCCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGc             >  5:74468/1‑135 (MQ=255)
                                                                                                                    tACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGc             >  6:363045/1‑135 (MQ=255)
                                                                                                                       gCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAATGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGcc            <  5:24418/133‑1 (MQ=255)
                                                                                                                       gCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGcc            <  2:121083/133‑1 (MQ=255)
                                                                                                                       gCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGcc            <  8:22199/133‑1 (MQ=255)
                                                                                                                       gCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGCCt           <  3:323717/134‑1 (MQ=255)
                                                                                                                              ccGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGAc                                  <  2:202168/104‑1 (MQ=255)
                                                                                                                              ccGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGAc                                  >  1:202168/1‑104 (MQ=255)
                                                                                                                              ccGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCCGCCCTTGCCTACCCCAg    >  1:391052/1‑134 (MQ=255)
                                                                                                                              ccGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGCCTACCCCAg    >  5:75847/1‑134 (MQ=255)
                                                                                                                               cGGCAATCAAACCCACCGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGCCTACCCCAGtt  >  4:17054/1‑135 (MQ=255)
                                                                                                                               cGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGCCTCCCCCAGtt  >  2:22533/1‑135 (MQ=255)
                                                                                                                               cGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGCCTACCCCAGtt  >  3:356842/1‑135 (MQ=255)
                                                                                                                                |                                                                                                                                     
AGAACCTGGGTGTCAGCGAACGTGCCGCGCACATGCCCGAAGGGCAGCCGGTTAGCCTGTTCCGGATTACCGCCGTCAGCAAGCAAAGCCGCTCACGGCATGTGCTGGAAGCGGTCTACGCCATGGCCGGCAATCAAACCCAACGCATCACCTGGCGACAAAGATTGCGAGGAGACTGACATGCAGCAAGGCTTGAGCCTGATCGAGTTGTTGATTGTGCTGGCCGTGACCGGCATTCTGGCAGCCATTGCCTACCCCAGTT  >  KT2440/709759‑710020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑