Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 812,764 G→T 6.0% R65L (CGT→CTT)  ada → Bifunctional transcriptional activator/DNArepair enzyme Ada

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440812,7640GT 6.0% 189.0 / 4.3 67R65L (CGT→CTT) adaBifunctional transcriptional activator/DNArepair enzyme Ada
Reads supporting (aligned to +/- strand):  ref base G (30/30);  new base T (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01

AGGCCACTTTGTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGCCAACTCAGTGCACGGCTGGCCGTCAGCC  >  KT2440/812636‑812898
                                                                                                                                |                                                                                                                                      
aGGCCACTTTGTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCtt                                                                                                                                   <  7:357768/134‑1 (MQ=255)
 ggCCACTTTGTCTACGCCGTACGTAACACCGGCGTCTACTGACACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGCCACTGCCGCGGCTGCCGCGGCTGCCCGCAACcgtgcgtg                                                                                                                                  >  4:91476/1‑134 (MQ=255)
   ccACTTTGTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGTCGGCTACCGTGCTTGCaa                                                                                                                               <  3:155068/135‑1 (MQ=255)
   ccACTTTGTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCaa                                                                                                                               <  1:179289/135‑1 (MQ=255)
   ccACTTTGTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCaa                                                                                                                               <  1:325030/135‑1 (MQ=255)
   ccACTTTGTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCaa                                                                                                                               <  2:203908/135‑1 (MQ=255)
    cACTTTGTCTACGCCGTACGTACCACCGGCGTCTACTGCCCCCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGCCACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCaaa                                                                                                                              >  4:110873/1‑135 (MQ=255)
    cACTTTGTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCaaa                                                                                                                              >  4:36001/1‑135 (MQ=255)
    cACTTTGTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCCGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACAGCCGCGGCTGCCCAGGCAGCCGGCTACCCTGCGTGCaaa                                                                                                                              >  5:335106/1‑135 (MQ=255)
      cTTTGTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACCTCCAGTTCTACGCCACTGCCGCGGCTGCCGAGGCTTCGCGCGACCGTGCGTGCAAAc                                                                                                                             >  7:157423/1‑134 (MQ=255)
          gTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCt                                                                                                                          <  3:66332/133‑1 (MQ=255)
          gTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCt                                                                                                                          <  2:281993/133‑1 (MQ=255)
          gTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGGTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGt                                                                                                                        >  4:176877/1‑135 (MQ=255)
          gTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTAAGAACGTCGAGTTCTGCGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCGTGCAAACGCTGt                                                                                                                        >  7:294097/1‑135 (MQ=255)
                gCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATc                                                                                                                     <  1:346465/132‑1 (MQ=255)
                gCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGc                                                                                                                  <  4:301456/135‑1 (MQ=255)
                 ccGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAg                                                                                                                   >  7:31681/1‑133 (MQ=255)
                   gtacgtacCACCGGCGTCTACTGCCCCCCCGGCTGCAAATCACGCCCGGCCAAGCGTATGAACGTCGAGGTCTACGCCACTGCCGCGGCGGCCGCGGCTGCCGGCGACCGTGGTTGCACACGCGGGATCAGCacg                                                                                                               >  4:11471/1‑133 (MQ=255)
                       gtacCCCCGGCGTCTACTGCCACCCCGGCTGGAAATAACGCCAGGCCCAGCGTATGAACGTCGCGTTCTACGACACTGCCGCGGCTGCCCAGGCTGCCGGGGACCGTGCTTGCACACGCGGTATCAGCAAGGccc                                                                                                           >  8:197293/1‑134 (MQ=255)
                        tacCACCGGCGTCTACTGCCCCCCCGGCTGCAAATCAAGCCAGGCCAAGCGGATGAACGTCGAGTTCGACGACACTGCCGCGGCTGCCGCGGCTGCCGGCTACCGGGCGTGCCAACGCGGTATCAGCAAGGcccg                                                                                                          >  7:360413/1‑133 (MQ=255)
                        tacCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGCGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAg                                                                                                          >  3:164724/1‑135 (MQ=255)
                        tacCACCGGCGTCGACTGCCACCCCCGCGGCAAATCACGCCAGGCCCAGCGTATGAACGTCGAGTTATAAGACACTGCCCCGGCTGCCGCGGCTGCCGGCGACCGTGGTTGCAAACGCAGTATAAGCAAGGCCAg                                                                                                          >  2:346465/1‑135 (MQ=255)
                            accGGCGTCTACTGCCACCCCGGGGGCAAATCCCGCCAGGGCAAGCGTATGAACGTGGAGTTCTACGACACTGCCGCGGCTTCCGCGGCTGCCGGCTACCCTGCGTGCAAACGCTGTATCAGCAAGGCCCGCGcc                                                                                                      >  6:94521/1‑135 (MQ=255)
                                   tgtACTGCCACCCCGGCTGCAAATCACCCCAGGCCAAGCGTATTAACGTCGAGTTCTACGACACTGCCGCGGCTGCCCCGGCTGCCCGCTACCGGGCTTGCCAACGCGGTATCAGCCAGGCCAGCCCCACCCGcc                                                                                               >  2:164291/3‑135 (MQ=255)
                                      acagCCAACCCCGCAGCAAATCACGCCCGGCCAAGCGGATGAACGTCCAGTTCTACGACCCAGCCGCGGCTTCCCAGGCTGCCGGCTACCCTGCTTGCAAACGCTGTATCAGCAAGCCCACCGCCACCCCCcaca                                                                                            >  2:29281/4‑135 (MQ=255)
                                      aCTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGCGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCcaca                                                                                            >  7:184340/1‑135 (MQ=255)
                                        tGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCAcagc                                                                                          <  8:139100/135‑1 (MQ=255)
                                             ccccGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCt                                                                                     >  2:64931/1‑135 (MQ=255)
                                                cGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTgg                                                                                   >  6:189086/1‑134 (MQ=255)
                                                 ggCTTCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGtt                                                                                 <  3:335811/135‑1 (MQ=255)
                                                 ggCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGtt                                                                                 <  6:288123/135‑1 (MQ=255)
                                                     gCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCCGCTGGTTACTc                                                                             >  4:266178/1‑135 (MQ=255)
                                                              gCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGcc                                                                    <  7:306899/135‑1 (MQ=255)
                                                                 aGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCt                                                                 >  2:372562/1‑135 (MQ=255)
                                                                  ggCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCtt                                                                <  2:312866/135‑1 (MQ=255)
                                                                      aaGCGTATGAACGTCGAGTTCTACGACAGTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTGGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATc                                                            <  8:294097/135‑1 (MQ=255)
                                                                      aaGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATc                                                            <  8:31681/135‑1 (MQ=255)
                                                                                aCGTCGAGTTCTACGACACTGCCGCGGCTGCCTAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCg                                                  >  4:295638/1‑135 (MQ=255)
                                                                                aCGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCg                                                  >  1:2067/1‑135 (MQ=255)
                                                                                aCGTCGAGTGCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCg                                                  <  1:71127/135‑1 (MQ=255)
                                                                                 cGTCGAGTTCTACGACACTGCCGCGGCGGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGa                                                 <  2:18529/135‑1 (MQ=255)
                                                                                      aGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCg                                            >  1:1546/1‑135 (MQ=255)
                                                                                      aGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCAACAGCCAGCTGCTTAATCACGCCTGCCGCTTGATCGAAACCAGCGACCCCg                                            >  2:26111/1‑135 (MQ=255)
                                                                                       gTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGt                                           >  2:17764/1‑135 (MQ=255)
                                                                                              gccacTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCCGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGAGCGAACCCAGCGACCCCGTTCCaa                                      <  7:351019/131‑1 (MQ=255)
                                                                                              gACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTTTCAGCAAGGCCAGCGCCACCCGCCACAGCCAGGTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCaa                                      <  4:381429/133‑1 (MQ=255)
                                                                                              gACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCaa                                      <  1:64931/133‑1 (MQ=255)
                                                                                                 acTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTc                                 <  3:110873/135‑1 (MQ=255)
                                                                                                     ccGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCCGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGcc                             >  6:361418/1‑135 (MQ=255)
                                                                                                     ccGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAACCCAGCGACCCCGTTCCAAGCCTCGGcc                             >  6:363829/1‑135 (MQ=255)
                                                                                                     ccGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGc                              >  4:363308/1‑134 (MQ=255)
                                                                                                     ccGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGc                              >  2:229745/1‑134 (MQ=255)
                                                                                                      cgcggcggccgcggcggcCGGCTACCGGGCTGCCAACCGCTGTACCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCCCGCCTGCCGCTTGAGCGAACCCAGCGACCCCGTTCCAAGCCTCGGCCa                            <  1:148976/135‑1 (MQ=255)
                                                                                                      cgcgGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGtt                                                                                 >  3:112378/1‑82 (MQ=255)
                                                                                                      cgcgGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGtt                                                                                 <  4:112378/82‑1 (MQ=255)
                                                                                                      cgcgGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGCCa                            >  6:35826/1‑135 (MQ=255)
                                                                                                      cgcgGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGCCa                            <  6:330361/135‑1 (MQ=255)
                                                                                                      cgcgGCTGCCGAGGCTGCCGGCTACCGTGCGTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGCCa                            <  7:129065/135‑1 (MQ=255)
                                                                                                        cgGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGCCAAc                          >  5:315330/1‑135 (MQ=255)
                                                                                                           cTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATc                                                                                                                     <  3:24409/41‑1 (MQ=255)
                                                                                                           cTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATc                                                                                                                     >  4:24409/1‑41 (MQ=255)
                                                                                                                      gCCGGCTACCTTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGc                                                                         >  3:239980/1‑74 (MQ=255)
                                                                                                                      gCCGGCTACCTTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGc                                                                         >  3:239983/1‑74 (MQ=255)
                                                                                                                      gCCGGCTACCTTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGc                                                                         <  4:239980/74‑1 (MQ=255)
                                                                                                                      gCCGGCTACCTTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGc                                                                         <  4:239983/74‑1 (MQ=255)
                                                                                                                        cGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGCCAACTCAGTGCACGGCTGGc          <  4:332350/135‑1 (MQ=255)
                                                                                                                           cTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGCCAACTCAGTGCACGGCTGGCCGt       <  5:35826/135‑1 (MQ=255)
                                                                                                                           cTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGCCAACTCAGTGCACGGCTGGCCGt       <  3:36001/135‑1 (MQ=255)
                                                                                                                                gTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGACACCAGCGACCCCGTGCCAAGCCTCGGCCAACTCAGTGCACGGCTGGCCGTCAGcc  >  3:52937/1‑135 (MQ=255)
                                                                                                                                |                                                                                                                                      
AGGCCACTTTGTCTACGCCGTACGTACCACCGGCGTCTACTGCCACCCCGGCTGCAAATCACGCCAGGCCAAGCGTATGAACGTCGAGTTCTACGACACTGCCGCGGCTGCCGAGGCTGCCGGCTACCGTGCTTGCAAACGCTGTATCAGCAAGGCCAGCGCCACCCGCCACAGCCAGCTGGTTACTCACGCCTGCCGCTTGATCGAAACCAGCGACCCCGTTCCAAGCCTCGGCCAACTCAGTGCACGGCTGGCCGTCAGCC  >  KT2440/812636‑812898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: