Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	924,333	T→G	25.8%	D4780A (GAC→GCC)	CJ019_00881 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	924,333	0	T	G	25.8%	82.1 / 2.8	58	D4780A (GAC→GCC)	CJ019_00881	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/13);  new base G (7/10);  total (35/23)
Fisher's exact test for biased strand distribution p-value = 7.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCA  >  KT2440/924201‑924467                                                                                                                                      |                                                                                                                                       cTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTcg                                                                                                                                      >  8:269618/1‑135 (MQ=255)      aTCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCGCGCCCAGGCCGCTGACCACGCTGCCGGCGGC‑GTTGATCACCACTTGGGTTAGTGGGTcgggcgg                                                                                                                                 <  1:290378/135‑1 (MQ=255)           ccAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCCGGCCGCTGACCACGCTGCCGTCGGC‑GGTGGTCACCACTTGGGTGAGTGGGTCGGGCGGGGGGa                                                                                                                            >  8:90144/1‑135 (MQ=255)             aGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAg                                                                                                                          <  2:297079/135‑1 (MQ=255)                aCGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTcc                                                                                                                       <  5:340601/135‑1 (MQ=255)                       ccGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCCGGCCGCTGACCCCGCTGCCGTCGGG‑GGTGCTCACCACGTGGGTGGGTGGGGCGGGCGGGGTGGAGGCCGGGGgtt                                                                                                                >  4:25639/1‑135 (MQ=255)                            cccAGTACGGTACCGGCCGCGTTACTGCCGCTTACCGTGGCGCCGGCCTCGCCCCGGCCGCTGCCCCCGCGGCCGGCGGC‑GGTGATCACCCCATGGGTGAGGGGGGCGGGCGGGGGGGAGTCCGGGGGTTggctg                                                                                                           >  5:82826/1‑131 (MQ=255)                            cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCCGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGGGAGTGGGGCGGGCGGGGTGAAGTACGGGGTTTGCAgg                                                                                                           >  4:48216/1‑135 (MQ=255)                            cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGGCGGGCGGGGGGAAGTCCGGGGGTTGCAgg                                                                                                           >  8:5404/1‑135 (MQ=255)                            cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCGGACCACGCTGCCGTCGGCGGTTAAGCACCACTGGGTTGAGGGGGGCGGG‑GGGGGGGAGTGCCGGGTTTggcgg                                                                                                           >  8:75349/1‑131 (MQ=255)                              cAGTACGGTCCCGGCCGCGTTACTGATGATTACGGTGGCGCCGGCCGCGCCCAGGCGGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTATGCAGGGt                                                                                                         <  7:311277/135‑1 (MQ=255)                               aGTACGGTACCGGCCGCGTTACTGACGATTACCGTGTCCCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GGTGATCACCACGGGGGTGAGTGGGTCGGGGGGGGGGAAGTCCGGGGGTTGGAgggga                                                                                                        >  7:188193/1‑133 (MQ=255)                                    ggTACCGGCCGCGTTACTGACGATTACCGTTGCGCCGGCCTCGACCACGCCGCAGACAACCCAGCCGACGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCAc                                                                                                   >  3:155542/1‑135 (MQ=255)                                    ggTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCGGCCCACGCTGCCGGCGGC‑GGTGGTCACCACTTGGGGGTGGGGGTGGGGGGGGGGGAAGTGCGGGGGTTGGCGGGTTGcccc                                                                                                   >  4:383861/1‑133 (MQ=255)                                         cGGCCGCGTTACTGCCGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTTCCGTCGGC‑TGTGATACCCACGTGGGTGAGGGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGTGGAGCCACTGcag                                                                                              >  5:254578/1‑133 (MQ=255)                                         cGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCg                                                                                              <  1:220739/135‑1 (MQ=255)                                         cGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCGGCCCACGCTGCCGTCGGC‑GGTGATCACCACTTGGGTGTGGGGGTCGGGCGGGGGGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCg                                                                                              >  8:87589/1‑135 (MQ=255)                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCCGGCCGCTGACCCCGCAGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGGCCGGGGTTTGGAGGGTAGCCCCTGCCGCCACg                                                                                         >  3:105969/1‑135 (MQ=255)                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCACGGTAGCCACTTCCGACACg                                                                                         >  2:244990/1‑135 (MQ=255)                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCGGACCACGCTGCCGTCGGC‑GTGGCTACCCCCGTGGGGGAGTGGGTGGGGGGGGGGGCAGTCCGGGGTTTGGAGGGTTGCCACTGCCgccccg                                                                                         >  4:26992/1‑130 (MQ=255)                                              gcgTTACAGACGATTAACGTGGCGCCGCCCTCCCCCCCGCCGCAGACCACCCAGCCCTCCGC‑GTTGATCAACACGAGTGTGACTGGGTCGGGCGGGGTGAATTGCCGGGTTTGCATGGTAGCCAAGGCCCACACg                                                                                         >  2:214488/1‑135 (MQ=255)                                                  tACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGGGAGTGGGTCGGGCGGGGTGAAGTCCGGGGGTTGCAGGGTAGCCACTGCCGACACGTTgc                                                                                     >  8:313739/1‑135 (MQ=255)                                                   tcTGACGATTACCGTGGCGCCGGCCTCGCCCACGCCGCGGACCACGCTGCCGGCGGC‑GGTGATCACCACATGGGGGAGGGGGTGGGGCGGGGTGAAGTCCGGGGGTTGCAGGGTAGCCACTGCCGACACGGTgcc                                                                                    >  3:81226/2‑135 (MQ=255)                                                   aCTGACGATTACCGTGGCGCCGGCCACGCCCCGGCCGCAGACCACGCTGCCGGCGGC‑GGTGATAACCCCCAGGGTGGGTGGGGCGGGGGGGGTGGAGTCCCGGGTTTTCAGGGTTGCCACAGGCGCCACgattcc                                                                                    >  2:276740/1‑130 (MQ=255)                                                        cGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTgccggcc                                                                                <  2:6593/134‑1 (MQ=255)                                                            tACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATc                                                                           <  7:292890/135‑1 (MQ=255)                                                            tACCGGGGCGCCGGCCGCGCCCAGGCCGCTGACCACGCTGCGGTCGGC‑GTTGATCACCACTTGGGGGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATc                                                                           <  3:292823/135‑1 (MQ=255)                                                             ccgggggCGCGGGCCCCGCCCCGGCCGCTCCCCCCGCGGCCGGCGGG‑GTGGAGCACCCCTGGGGGGCGTGGGGGGGGCGGGGGGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATc                                                                           <  5:258417/129‑1 (MQ=255)                                                                      ccGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGt                                                                  >  3:357260/1‑134 (MQ=255)                                                                           cTCGCCCAGGCCGGGGACCACGCGGCCGTCGGG‑GTGGATCCCCAGTTGGGGGAGTGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCt                                                            <  6:94736/135‑1 (MQ=255)                                                                           cTCGCCCAGGCCGCTGACCACGCTGCCGGCGGC‑GTGGAGCACCAGTTGGGTGAGGGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCt                                                            <  5:41391/135‑1 (MQ=255)                                                                                caaGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGagca                                                       <  2:243312/133‑1 (MQ=255)                                                                                 cAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGagcag                                                      >  7:128869/1‑135 (MQ=255)                                                                                  cggCCGCTGACCACGCGGCGGGCGGC‑GTTGATCCCCACTGGGGGGAGGGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGagcagc                                                     <  6:82826/134‑1 (MQ=255)                                                                                     ccGCTGACCACGCTGCCGGCGGG‑GTTGATCACCACTTGGGTGAGTGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCg                                                  <  1:257526/135‑1 (MQ=255)                                                                                     ccGCTGACCACGCTGCCGGCGGC‑GTGTATCACCACTTGGGTTAGTGGGGCGGGCGGGGGGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCg                                                  <  1:214488/135‑1 (MQ=255)                                                                                     ccGCGGCCCCCGCGGCCGGCGGG‑GTGGAGCACCACTTGGGGGAGGGGGTCGGGCGGGGTGAAGGCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCg                                                  <  1:361630/135‑1 (MQ=255)                                                                                          gcccACGCGGCCGGCGGC‑GTGGAGCCCCCCTTGGGGGAGTGGGGCGGGCGGGGTGAAGTCCGGGGGTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGtt                                             <  5:360698/133‑1 (MQ=255)                                                                                          gACCACGCTGCCGGCGGC‑GTTGATCCCCACTTGGGTGCGGGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGGAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGtt                                             <  1:333074/135‑1 (MQ=255)                                                                                           aCCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTg                                            >  6:330843/1‑135 (MQ=255)                                                                                           aCCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTg                                            >  8:32548/1‑135 (MQ=255)                                                                                                gCTGCCGTCGGC‑GTTGATCACCACTGGGGTGAGGGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAg                                       <  3:94241/135‑1 (MQ=255)                                                                                                   gCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGc                                    >  2:290661/1‑135 (MQ=255)                                                                                                     cGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCg                                  >  6:273238/1‑135 (MQ=255)                                                                                                      ggcGGC‑GTTGAGCACCACTGGGGGGAGGGGGTCGGGCGGGGGGAAGTCCGGGGGTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCgg                                 <  1:160474/133‑1 (MQ=255)                                                                                                        cGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGc                               >  3:371984/1‑135 (MQ=255)                                                                                                         ggC‑GTTGATCACCACTTGGGGGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCt                              <  8:60305/135‑1 (MQ=255)                                                                                                          gC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCTGCGTTGCCCAGTGCCGGGc                               >  2:350335/1‑133 (MQ=255)                                                                                                           c‑GTTTATCACCACTTGGGGGTGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCa                            <  4:81226/135‑1 (MQ=255)                                                                                                           c‑GTTGAGCGCCGCTTGGGGGTGTGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACAGGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTGGCCCAGTGCCGGGCTCa                            <  2:179637/135‑1 (MQ=255)                                                                                                             gTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAg                           >  7:165043/1‑135 (MQ=255)                                                                                                              ttGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTGCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGCGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGc                          >  2:301428/1‑135 (MQ=255)                                                                                                                  tCACCACGTGGGTGAGTGGGTCGTGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTAGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTAGCCCAGTGCCGGGCTCAGCGTGa                      >  8:181571/1‑135 (MQ=255)                                                                                                                                   gggTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCAcag     >  5:61057/1‑133 (MQ=255)                                                                                                                                   gggTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTg     >  4:222512/1‑135 (MQ=255)                                                                                                                                   gggTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTg     >  6:195493/1‑135 (MQ=255)                                                                                                                                    ggTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTg     >  5:336113/1‑134 (MQ=255)                                                                                                                                     gTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGc   >  5:275105/1‑135 (MQ=255)                                                                                                                                      tCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGCGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGATGTGGCCACTGGCa  >  6:76501/1‑135 (MQ=255)                                                                                                                                      |                                                                                                                                       CTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGC‑GTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCA  >  KT2440/924201‑924467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑