| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,266,383 | T→G | 49.1% | intergenic (+121/+47) | cysK_1 → / ← CJ019_01185 | Cysteine synthase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,266,383 | 0 | T | G | 49.1% | 2.6 / 3.8 | 29 | intergenic (+121/+47) | cysK_1/CJ019_01185 | Cysteine synthase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (7/7); new base G (10/3); total (17/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCG > KT2440/1266268‑1266513 | tgtgTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAt > 6:82343/1‑135 (MQ=255)tgtgTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATAGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCCCACTGTTAGAGccca > 2:236079/1‑133 (MQ=25) aTGATCCAGAGGCTGGCACGGAAACTGTGGGAGAGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTAGAGCCATCTCTCAGAatt > 8:212053/1‑134 (MQ=255) aTCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCTCGGGCGCGCCCGCTCCCACAGTGTTAGAGCCAGCTCTCAGAttttttt > 7:225828/1‑133 (MQ=255) aTCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGGGCGCCCGCGCCCCCAGAGTTCGCGCCATCTCTCACAatttttt > 7:316119/1‑133 (MQ=25) aTCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTAt > 8:383868/1‑135 (MQ=255) aTCCAGAGGCTGGCACGGAAACTGTGGGAGAGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCGCCCACAGTGTTCGCGCCATCTCTCAGAttttt > 3:53444/1‑133 (MQ=255) agGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCGCCCCCCGGGTTCGCGCCCTCTCGCAGGtgtttttgggg > 6:281914/1‑127 (MQ=18) gCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTGGCGGGCGCGCCCGCGCCCCCAGGGGTCGAGCCCGCGCGCAGCGTTTTATggcggggg > 3:125430/1‑131 (MQ=18) gCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCGCCCCCAGGGTTGGTGCCCTCGCTCAGCTTTTTATGGGGTCAg > 1:44303/1‑135 (MQ=21) gCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCCCGCCCCCCGGGGTGGGGGCCGCTCGGAGATTTTTATAGCGTCAg > 5:175484/1‑135 (MQ=21) aCGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGGGCGCCCCCGGCCACACTGTTGGCGCCCTCGCTCAGATTTTTATGGGGTTCGTCGa > 8:266216/1‑133 (MQ=21) ggAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCGCCGCCACTGTTCGAGCCAACGCTCAGAATTTTATAGCGGCAGTCGATccg > 8:377329/1‑133 (MQ=25) gggAGCGGGCACGCCCGCGAAGCTGGCGACGCGGTGGAGGGCACGGGCTTCGCCCGTGTGCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTgg < 6:384146/135‑1 (MQ=255) gCACGCCCGCTACGCCGGCGACGCGGTGGATGGCCCGGGCTGCGCCGGGGTGCGCGGGCGCGCCCGCGCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCActtctt < 4:89930/135‑1 (MQ=255) aGCCCGGGAAGCCGGCGACGCGGTGGATGGCACGGGCGTCGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTa < 8:297157/135‑1 (MQ=255) tGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATcgcg > 8:303772/1‑76 (MQ=255) tGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATcgcg < 7:303772/76‑1 (MQ=255) gCACGGGCTGCGCCCGGGGTCGCGGGGGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGt < 4:82162/135‑1 (MQ=255) cTTCGCCCGTGTTCGCGGGCGCGCCCGCGGCCCCAGGGTTGGAGGCCTCTCTCCGCTTTTTATAGGGGGCGGGGGTCAGGCGCCGGGGGCCGTGGTGGACGTATTATCGCTACCGCCCGGGCCCGGGGCGTTTCg > 6:200833/1‑135 (MQ=255) ggcGCCGGTGTGGGGGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACgg < 5:200833/133‑1 (MQ=255) gCCGGTGTGCGGGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGcc < 2:57624/134‑1 (MQ=255) gCCCGTGTTCGCGGGCGCGCCCGCGCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGACa < 8:137808/135‑1 (MQ=255) tCGCGGGCGCGCCCGCTCCCCCAGGGTTCGCGCCCTCGCGCAGAGTTTTATCGCGGCCGTCGCTCAGGCGCCGGGGCCCGGCTTGTACTTCTTCTCGCTTCCGCACCGGCACGGGTCGGTACGGCCGACGTCCt > 6:120982/1‑134 (MQ=255) gcgcgcCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGc < 8:215016/135‑1 (MQ=255) cgcgcCCGCCCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCg < 1:71362/135‑1 (MQ=255) ccGCGCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCg < 6:254736/135‑1 (MQ=255) ccGCGCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATAGCGTCAGTCGATAAGGCGCCGTGGCACTTATTTTTCGTCATCTAGCAAACGCCAGTGGACCGGTCGTTAAGGCCCACCTCAGACAGGGCGTTggg > 8:250990/1‑133 (MQ=255) ccGCCCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCg < 5:47146/135‑1 (MQ=255) | TGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCG > KT2440/1266268‑1266513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |