| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,317,342 | C→A | 14.6% | A248S (GCA→TCA) | yfdC ← | Inner membrane protein YfdC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,317,342 | 0 | C | A | 14.6% | 136.4 / 2.8 | 62 | A248S (GCA→TCA) | yfdC | Inner membrane protein YfdC |
| Reads supporting (aligned to +/- strand): ref base C (21/31); new base A (2/8); total (23/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCGGACCGGGGCTGGTCTTCGTTCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCAGGTAGGTGATCATCAGAATGATCCAG > KT2440/1317210‑1317472 | gcgcgGACCGGGGCTGGTCTTCGTTCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCaa < 4:324847/135‑1 (MQ=255) cgcgGACCGGGGCTGGTCTTCGTTCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCaaa < 4:324922/135‑1 (MQ=255) ggACCGGGGCTGGTCTTCGTTCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCCCCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAAtt > 2:140368/1‑134 (MQ=255) ggACCGGGGCTGGTCTTCGTTCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAAtt > 7:306386/1‑134 (MQ=255) ggACCGGGGCTGGTCTTCGTTCAGAAGCCGCGAGGGTGGTTAGCCCCTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTc > 8:270522/1‑135 (MQ=255) ggggCTGGTCTTCGTTCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTccag > 5:297846/1‑133 (MQ=255) ggCTGGTCTTCGTTCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTccagccag < 7:329003/135‑1 (MQ=255) ggCTGGTCTGCGTCCAGAAGCGGCGTGGGGGGTTTGCCACGGTCACTGCGCACTTGTGCTTGGCTGATCAAGGCGACGATTAAACTGCCACCGATGATGTGCCCCGCCAGCGTCGGCAGTGAAAATTccacccag < 4:278319/135‑1 (MQ=255) ttCGTTCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCt > 5:70782/1‑135 (MQ=255) tCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAgg > 2:262418/1‑135 (MQ=255) tCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAgg > 3:388079/1‑135 (MQ=255) tCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAgg > 7:254398/1‑135 (MQ=255) gCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTtcgt < 7:180134/135‑1 (MQ=255) gCCGCGAGGGGGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGACGCGGCTCCAGGTTtcgt < 4:11443/135‑1 (MQ=255) gtggtTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGccc < 1:98201/135‑1 (MQ=255) gtggtTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGccc < 7:97035/135‑1 (MQ=255) gtggtTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGccc < 3:200532/135‑1 (MQ=255) gtggtTTGCCACTGTCACGGCGCACTGGTGCATGGCGGAGAACGGCGAAGATGAACCTGCCACCGAGGATGTGGCCCGCCAGCGGCGGCAGTGAAAATTCCCGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGccc < 4:75734/135‑1 (MQ=255) gtggtTTGCCACTGTAACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCTGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGccc < 8:222584/135‑1 (MQ=255) gtggtTTGCAACTGTCAGTGCGCACTTGTGCATGGCTTATCAAGGCGAAGATGAAAGTGCCACCGATTCTGTTGCCCGCCAGCGTGGGCTGTGAAATTTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGccc < 5:100144/135‑1 (MQ=255) ccACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAACCTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAgg < 5:79348/135‑1 (MQ=255) cACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAg > 7:352021/1‑133 (MQ=255) aCTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGGCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTa < 3:82481/135‑1 (MQ=255) gTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAgg > 1:15577/1‑134 (MQ=255) cgcACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCTGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCg < 2:81886/135‑1 (MQ=255) cgcACGTGGCCATGGCTGATCACCGCAACTCTCAAACTGCCCCCGCTGAGGTTGCCCGCCGGCGTCGGCAGTGCAAATTCCACCCAGAAGCGGCTCCAGGTTGCGGCGCCAGCCCACACCAGGTAGGACACTTCg < 1:185596/135‑1 (MQ=255) gcACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCg < 1:249202/134‑1 (MQ=255) gcACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGa > 2:260755/1‑135 (MQ=255) gcACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCAACCGATGATGTTGCCCTCCAGCGTCTGCAGTGAAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTATGACACTTCGa > 4:363666/1‑135 (MQ=255) ttGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGa > 6:136995/1‑135 (MQ=255) ttGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGa < 8:260145/135‑1 (MQ=255) ttGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGa > 6:351790/1‑135 (MQ=255) ttGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGa > 1:239801/1‑135 (MQ=255) ttGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGa < 2:116313/135‑1 (MQ=255) ttGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGGCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGa < 7:249047/135‑1 (MQ=255) ttGTGCAGGGCTGAGAAAGGCGAAGATAAACCTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGAAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGa < 6:257000/135‑1 (MQ=255) gtgCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGCCTGAcc > 5:191231/1‑135 (MQ=255) gtgCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGAca > 3:46770/1‑134 (MQ=255) gCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGAccc < 8:63369/134‑1 (MQ=255) aTGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGAAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCcca < 4:135919/134‑1 (MQ=255) aTCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACtata > 2:25637/1‑135 (MQ=255) aTCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACtata > 8:330982/1‑135 (MQ=255) aaGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGa > 1:49657/1‑135 (MQ=255) aaGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTACGACAGCCCCCCCCACTATATGa > 2:88764/1‑135 (MQ=255) tGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGc < 3:164836/135‑1 (MQ=255) tGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGAAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTAGATGAGTGAAATCg > 3:383785/1‑134 (MQ=255) gAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGc < 1:25637/134‑1 (MQ=255) cACGGAGGATGTTGCCCGCCAGCGGCGGCAGTGCAAATTCCAGCCAGACGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGc < 6:297846/133‑1 (MQ=255) cGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGGTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCa < 8:306386/135‑1 (MQ=255) tgTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCaggtag < 1:94806/134‑1 (MQ=255) tgTTGCCCGCCAGCGTCGGAAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCaggtag < 4:23051/134‑1 (MQ=255) tgTTGCCCGACAGGGTCTGCAGTGAAAATTACATCCATAAGCGGCTCCTGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCaggtag < 3:363666/134‑1 (MQ=255) cccGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCAGGTAGGTGat < 1:260755/134‑1 (MQ=255) cccgcgcCGGCAGTGAAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCAGGTAGGTGAtcatca < 1:164247/132‑1 (MQ=255) cccgCGTCGGCAGTGAAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCAGGTAGGTGAtcatca < 1:140368/132‑1 (MQ=255) ccAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCAGGTAGGTGAtcatca < 1:18117/135‑1 (MQ=255) ccAGCGTCGGATGTGCAATTTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCAGGTAGGTGAtcatca < 8:86221/135‑1 (MQ=255) tCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCAGGTAGGTGATCATCAGAATGa < 2:15577/135‑1 (MQ=255) tCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCAGGTAGGTGATCATCAGAATGa < 2:49657/135‑1 (MQ=255) tCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCAGGTAGGTGATCATCAGAATGa < 3:336842/135‑1 (MQ=255) aGTGCAAATTCCAGCCAGACGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCAGGTAGGTGATCATCAGAATGATCCAg < 5:381797/135‑1 (MQ=255) gTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGGCGCCAGCCCACACCAGGTAGGACACTTCGACTGCCCCCACCACTATATGAGTGAAATCGCCAAGGGCCCTCAGGTAGGTGATCATCAGAATGAt > 3:65178/1‑130 (MQ=255) | GCGCGGACCGGGGCTGGTCTTCGTTCAGAAGCCGCGAGGGTGGTTTGCCACTGTCACTGCGCACTTGTGCATGGCTGATCAAGGCGAAGATGAAACTGCCACCGATGATGTTGCCCGCCAGCGTCGGCAGTGCAAATTCCAGCCAGAAGCGGCTCCAGGTTTCGTCGCCAGCCCACACCAGGTAGGACACTTCGACTGACCCCACCACTATATGAGTGAAATCGCCAAGGGCCATCAGGTAGGTGATCATCAGAATGATCCAG > KT2440/1317210‑1317472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |