Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,563,328 C→T 5.9% S361S (AGC→AGT)  pcaF_1 → Beta‑ketoadipyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,563,3280CT 5.9% 212.8 / 2.4 67S361S (AGC→AGT) pcaF_1Beta‑ketoadipyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base C (22/41);  new base T (2/2);  total (24/43)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGTGATCGAGCTTAACGAAGCGTTTGCCAGCCAAGGCCTGGCGGTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCT  >  KT2440/1563194‑1563461
                                                                                                                                      |                                                                                                                                     
gACGTGATCGAGCTTAACGAAGCGTTTGCCAGCCAAGGCCTGGCGGTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGc                                                                                                                                       >  6:327088/1‑135 (MQ=255)
  cgggctCGAGCTTAACGAAGCGTGAGCCAGCCAAGGCCTGGCGGTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCgg                                                                                                                                     <  8:337343/130‑1 (MQ=255)
  cGTGATCGAGCTTAACGAAGCGTTTGCCAGCCAAGGCCTGGCGGTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCgg                                                                                                                                     <  7:376513/135‑1 (MQ=255)
  cGTGAGCGAGCTTCACGAAGCGTTTGCCAGCCCAGGCCTGGCGGTGCTGCGTGAGCTGGGTGGGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCgg                                                                                                                                     <  5:60059/135‑1 (MQ=255)
       tCGAGCTTAACGAAGCGTTTGCCAGCCAAGTCCTGGCGTTGCTGCGTGAGCTGGGGGTGGCTGACGATGCGCACCATTTAAACCCTAATGGCGGAGCCATTGCCCTGGGCCACCCCCTGTGCATGAGCGGTGCa                                                                                                                                 <  5:140336/134‑1 (MQ=255)
       tCGAGCTTAACGAAGCGTTTGCCAGCCAAGGCCTGGCGGTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCa                                                                                                                                 <  5:260020/134‑1 (MQ=255)
       tCGAGCTTAACGAAGCGTTTGCCAGCCAAGGCCTGGCGGTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCAc                                                                                                                                <  7:102771/135‑1 (MQ=255)
                 cGAAGCGTTTGCCAGCCAAGGCCTGGCGGGGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTac                                                                                                                       <  6:75789/134‑1 (MQ=255)
                 cGAAGCGTATGCCAGCCAAGGCCTGGCGGTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCATGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCATGGGCATGAGCGGTGCACGCCTGGTac                                                                                                                       <  7:203140/134‑1 (MQ=255)
                    aGCGTTTGCCAGCCAAGGCCTGGCGGTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGT‑AACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTactgact                                                                                                                  <  6:132544/135‑1 (MQ=255)
                             cagccaAGGCCTGGCGGTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGc                                                                                                           <  7:354903/134‑1 (MQ=255)
                              agccaAGGCCTGGCGGTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGTCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCAc                                                                                                         <  5:211095/135‑1 (MQ=255)
                                    ggCCTGGCGGGGCTGCGTGAGCGGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTg                                                                                                   <  8:173915/135‑1 (MQ=255)
                                             gTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCTCCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAATAGt                                                                                          >  3:60868/1‑135 (MQ=255)
                                              tgctgcGTGAGCTGGGTGTGGCGGACGAGGCGCACCAGGTAAACCCTAATGGCGGTGCCATTGCACTGGGCCACCCCATGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTg                                                                                         <  4:257291/135‑1 (MQ=255)
                                                  gcGTGAGCTGGGTGTGTCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCgg                                                                                     >  7:9830/1‑135 (MQ=255)
                                                    gTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTc                                                                                   <  5:321326/135‑1 (MQ=255)
                                                    gTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTc                                                                                   <  1:130350/135‑1 (MQ=255)
                                                        gCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCt                                                                                                    <  6:104226/114‑1 (MQ=255)
                                                        gCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCt                                                                                                    >  5:104226/1‑114 (MQ=255)
                                                           gggTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAggg                                                                            >  6:257358/1‑135 (MQ=255)
                                                              tgtgGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCt                                                                         <  3:36815/135‑1 (MQ=255)
                                                              tgtgGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCt                                                                         <  4:214841/135‑1 (MQ=255)
                                                              tgtgGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCt                                                                         <  1:264070/135‑1 (MQ=255)
                                                                tgGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTgg                                                                       >  3:70460/1‑135 (MQ=255)
                                                                 ggCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTgg                                                                       <  3:317552/134‑1 (MQ=255)
                                                                  gCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGTGCCACCCCCTGGGCATGAGTGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCg                                                                     >  2:84216/1‑135 (MQ=255)
                                                                  gCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGTGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCg                                                                     >  2:89772/1‑135 (MQ=255)
                                                                  gCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCg                                                                     >  5:104658/1‑135 (MQ=255)
                                                                    tgccGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGGCGCAAGGGCCTGGCGAc                                                                   <  4:110431/132‑1 (MQ=255)
                                                                    tGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCTGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGAc                                                                   <  8:342570/135‑1 (MQ=255)
                                                                    tGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGAc                                                                   <  5:191241/135‑1 (MQ=255)
                                                                    tGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGAc                                                                   <  4:183867/135‑1 (MQ=255)
                                                                    tGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGAc                                                                   <  6:104658/135‑1 (MQ=255)
                                                                    tGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGAc                                                                   <  3:44276/135‑1 (MQ=255)
                                                                     gACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGAcc                                                                  >  8:159951/1‑135 (MQ=255)
                                                                     gACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGGGGCGGTCGCAAGGGCCTGGCGAcc                                                                  <  3:254551/135‑1 (MQ=255)
                                                                         aTGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCAtgt                                                              >  3:175126/1‑135 (MQ=255)
                                                                         aTGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCAtgg                                                              >  5:279561/1‑134 (MQ=255)
                                                                          tGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCAtgtg                                                             <  1:204934/135‑1 (MQ=255)
                                                                               cccAGGTATACCCTTATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCTTGAGCGGTGCACGCCTGGTACTGACTGCGTTGCTCCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTgg                                                        <  7:257533/135‑1 (MQ=255)
                                                                               cccAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTgg                                                        <  7:159951/135‑1 (MQ=255)
                                                                                ccAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTggg                                                       <  7:158364/135‑1 (MQ=255)
                                                                                     tAAACCCTAATGGCGGTGCGATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCg                                                  <  4:278830/135‑1 (MQ=255)
                                                                                                 gCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTgg                                      >  7:45536/1‑135 (MQ=255)
                                                                                                       ccATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTgg                                >  2:10440/1‑135 (MQ=255)
                                                                                                       ccATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTgg                                >  2:65039/1‑135 (MQ=255)
                                                                                                       ccATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTgg                                >  4:325229/1‑135 (MQ=255)
                                                                                                       ccATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTgg                                >  4:123035/1‑135 (MQ=255)
                                                                                                       ccATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCAAGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTgg                                >  7:18904/1‑135 (MQ=255)
                                                                                                             cccTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATc                           <  3:210956/134‑1 (MQ=255)
                                                                                                             cccTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATc                           <  2:305795/134‑1 (MQ=255)
                                                                                                             cccTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATc                           <  1:65039/134‑1 (MQ=255)
                                                                                                                   gCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCg                      <  7:146695/133‑1 (MQ=255)
                                                                                                                   gCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCg                      <  5:327088/133‑1 (MQ=255)
                                                                                                                   gCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCg                      <  7:168455/133‑1 (MQ=255)
                                                                                                                     cACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGtt                  >  5:349908/1‑135 (MQ=255)
                                                                                                                     cACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCAAGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGtt                  >  5:292187/1‑135 (MQ=255)
                                                                                                                      aCCCCCTGGGCATGAGTGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGttt                 <  1:84216/135‑1 (MQ=255)
                                                                                                                      aCCCCCTGGGCATGAGTGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGGCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGttt                 <  1:89772/135‑1 (MQ=255)
                                                                                                                      aCCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGttt                 <  6:349908/135‑1 (MQ=255)
                                                                                                                          ccTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGAc                                                                   <  2:192246/81‑1 (MQ=255)
                                                                                                                          ccTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGAc                                                                   >  1:192246/1‑81 (MQ=255)
                                                                                                                                    aGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGCCCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGc    >  8:220211/1‑134 (MQ=255)
                                                                                                                                     gCGGTGCACGCCTGGTACTGACTGCGTTGCACCAg                                                                                                      >  3:343725/1‑35 (MQ=255)
                                                                                                                                     gCGGTGCACGCCTGGTACTGACTGCGTTGCACCAg                                                                                                      <  4:343725/35‑1 (MQ=255)
                                                                                                                                     gCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGccg  >  6:189962/1‑134 (MQ=255)
                                                                                                                                     gCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCct  >  7:108067/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GACGTGATCGAGCTTAACGAAGCGTTTGCCAGCCAAGGCCTGGCGGTGCTGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCT  >  KT2440/1563194‑1563461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑