| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,670,690 | A→C | 34.8% | intergenic (+27/‑104) | thrC_2 → / → CJ019_01569 | Threonine synthase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,670,690 | 0 | A | C | 34.8% | 17.9 / 2.4 | 35 | intergenic (+27/‑104) | thrC_2/CJ019_01569 | Threonine synthase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (18/3); new base C (10/3); total (28/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACC‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTATGTGTTGATTCA > KT2440/1670569‑1670819 | tCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACC‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCCGCCCCAACGtc > 8:96099/1‑133 (MQ=255) tGAAGAGCGTTGCACGGTCCTGGCCAACGACC‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGGGGCCGCGCCGCCCCCACGGCTCAGCCgtt > 5:20692/1‑135 (MQ=255) agCGTTGCACGGTCCTGGCCAACGACC‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCCGCCCCCACCTCTCAGCCGTTTccat > 4:229824/1‑133 (MQ=255) gccGGGCCCGGCCAACCCCCGTGACGGCTTTACTGGGTTTGGCCCCCCCGCAGGCCAACCGCGGAACGCCGCTTAAATGCCAGGGGGTTGGGGGCGGCCGCGCACCCCCCACGTCTCAGCCTTTTCCCTCCTGTc < 6:108342/133‑1 (MQ=255) cccaCGACC‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAAcct > 8:129617/4‑133 (MQ=255) cc‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAATCCGCTGTAATTGCATGGCGTTTGGGGCTTCACCGCAGCCCCACCGTCTCAGCCTTTTCCCTACAGTCTATCAGAAACAtacaat > 2:283961/1‑128 (MQ=255) cc‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGGGTCCGCGCCGCCCCCCCGTGTCAGCCGGTTTCCTCCGGTCGATCGGGAACAGACTAtt > 5:42921/1‑134 (MQ=255) cc‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCCGCCCCAACGTCTCACCCTTTTCCCTCCGGTCTATAAGAAAAAACCTATTc > 8:344574/1‑135 (MQ=255) cc‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCCACGGCGCAGCCTTTTACCTCCTGTCTATCAGAAACATCCTAt > 8:339054/1‑133 (MQ=255) cc‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCGTTTCCCTCCGGTCTATCAGAAACATCCTAt > 2:93435/1‑133 (MQ=255) cc‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACCTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTAt > 2:344750/1‑133 (MQ=255) cc‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACCGCTCAGCCTGTTCCCTCCTGTCGATCAGAAACATCCTAtt > 1:63850/1‑134 (MQ=255) aCAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCAGCCCCAACGTGTCAGCCTTTTCCCTCCAGGGGTTAAGAAACATCCTATTAACGCTGCCCGgc > 6:144594/1‑135 (MQ=255) cAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCGGGAGCGCCGCCCCCACCGGTCAGCCGTTTGCCTCCGGTCTATCAGAAACCACCAATTAAGCCGTCCCGgcg > 5:122259/1‑135 (MQ=255) aGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCCGCCCCAACCTCTACGCCGTTTCCCTACTGTCTATCAGAAACAAACTATTCAGCCTGCCGGgcgc > 6:359884/1‑135 (MQ=255) aGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTATCAGCCTTTTCCCTCCGGTCTATCAGCAACATCCTATTCAGCCTGCCGGgcgc > 8:369954/1‑135 (MQ=255) ggCTTTGTCAGCCAGCACGGCAACCGCGGAAAGCCGCTTTAATTGCAGGGCGTTGGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCa < 4:87780/135‑1 (MQ=255) gCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCAGCCCCAAAGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCaa > 4:91922/1‑135 (MQ=255) tttGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGGTGCCGCGCCGCCCCAACGTCTCAGCCGTTTCCCGCCAGGATTTAAGTAACAACCGATAAAGCCTGCCCGgcggccag > 1:353712/1‑129 (MQ=255) tGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCGATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCa > 7:52548/1‑134 (MQ=255) tCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATaa > 1:35025/1‑135 (MQ=255) cagccagcACGGCAACCGCGGCAAGCCGCTGTAATTTCATCGCGTTTGGGGCTGCCGCGCAGCCCCAACGTCTCCCCCTTTTCCCGCCTGTCTATAAGAAACAGCCAATACAGCCTGCCGGGCGCCAAGCCTaaa > 8:11211/1‑135 (MQ=255) cagccagcACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATaaa > 4:76558/1‑135 (MQ=255) cagccagcACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCCGCCCCCACGGCTCCGCCGTTTCCCTACGGTCTATCAGAAACAACCGATTCCGCCAGCCGGGCGCCAACCATaaa > 7:342534/1‑135 (MQ=255) cagccagcACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATaaa > 3:361778/1‑135 (MQ=255) agcACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTACCTCCTGTCTATAAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGc > 3:87780/1‑135 (MQ=255) ggCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGTGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGtc > 4:276731/1‑135 (MQ=255) ggaacgCCGCTGTAATGGCGGGCCTGGTGGGCCTGCGGCGCCGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCa < 2:273858/130‑1 (MQ=255) cacgCCGCTGAAATGGATTGGGGTTGGGGCCTGCTGCGACCCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAgg < 7:101721/132‑1 (MQ=255) cAAGCGGCTTTATTTCCAGGGCGTTGGGGGCTGAAGCGATGCCCAAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAgg < 2:43456/135‑1 (MQ=255) cAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCCACGTCTCAGCCGTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCa > 2:325371/1‑133 (MQ=255) tatggCAGGGCTTTGGGGGCGCCCGCGCCCCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCAt < 6:122259/130‑1 (MQ=255) aTTTCATGGGGTGTGGGGCGCGACCGCAGCCCAAACGTCTCTGACTTTTCCCTCCTGTGACTCAGTAACTTCCTATTAAGGGTGCAGGGCGGCAAGCATAATTTAGCCGGTCCTCCCATCTGGAGCTGGTCATg < 8:63244/134‑1 (MQ=255) cGTTTGGGGCTGCAGCGCCGCCCCAACGGCTCAGCCTTTTGCCTCCGGTCTATCAGAAACATCCTATTCAGCCGGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCCTCAGGCGCGGCTCATGCACCCGccc > 2:18414/1‑134 (MQ=255) cGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCCCCAGCCCt > 7:363123/1‑135 (MQ=255) agcgcagcCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTATGTGTTGATTCa > 8:328240/1‑135 (MQ=255) | TCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACC‑TGAAGGCTGTACAGGGCTTTGTCAGCCAGCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCCCTATGTGTTGATTCA > KT2440/1670569‑1670819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |