Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,720,951 T→G 33.4% D90A (GAC→GCC)  plsB ← Glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,720,9510TG33.4% 56.1 / 9.2 47D90A (GAC→GCC) plsBGlycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (19/11);  new base G (3/14);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 5.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGGGTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGG  >  KT2440/1720817‑1721080
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ccGGGGTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGt                                                                                                                                    <  4:32747/135‑1 (MQ=255)
   gggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgg                                                                                                                                 <  1:362036/135‑1 (MQ=255)
   gggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgg                                                                                                                                 <  4:292074/135‑1 (MQ=255)
   gggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgg                                                                                                                                 <  3:87229/135‑1 (MQ=255)
   gggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgg                                                                                                                                 <  7:154413/135‑1 (MQ=255)
    ggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCggg                                                                                                                                <  5:361541/135‑1 (MQ=255)
     gTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgggg                                                                                                                               <  4:217893/135‑1 (MQ=255)
                 aaCACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTagaa                                                                                                                   >  4:282706/1‑134 (MQ=255)
                  acacGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgggg                                                                                                                               <  3:218071/122‑1 (MQ=255)
                  acacGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgggg                                                                                                                               >  4:218071/1‑122 (MQ=255)
                   cacGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAgaagaa                                                                                                                >  5:319967/1‑135 (MQ=255)
                       cTGACTGTATTGAGCGGCGCAGCTTCTGCGGCCGGCGGGCTGCCGGCAGCCACCAGGCGCGCGAGCGGGGGGGGGGCGCCGCTTTTGGCCGGGCGGCCGCGCCAGTCCGGGTGGGGGGTCAGGTAGAAGAAcgcg                                                                                                            <  4:304230/135‑1 (MQ=255)
                       cTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGCGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAAcgcg                                                                                                            <  6:319967/135‑1 (MQ=255)
                       cTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCGGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGGCCTGGCGCCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAAcgcg                                                                                                            <  2:48256/135‑1 (MQ=255)
                         gACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACgcggc                                                                                                          >  8:26290/1‑135 (MQ=255)
                         gACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTACTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGCTACAAGAAcgccgc                                                                                                          >  2:83018/1‑135 (MQ=255)
                              gAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGAGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGtt                                                                                                     >  8:252378/1‑135 (MQ=255)
                                       gcgcATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGCACGCGGCGGGTTCCTGCAGTg                                                                                            >  3:237773/1‑135 (MQ=255)
                                          cATCTTCTGCGGCATCCTGGCTGCCGGCCGCCACCAGGCGCGCGCGCGGGGGGGGGGCGCCGCTTTTGCCCTGGCGGCCGACCCCGTCGGGGGCGGGGGACAGTTAGAAGAACGCGGCGGGTTCCTGCAGTGGGc                                                                                         <  4:168823/135‑1 (MQ=255)
                                           aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGCGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGcc                                                                                        >  2:45266/1‑135 (MQ=255)
                                           aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGcc                                                                                        >  4:105732/1‑135 (MQ=255)
                                           aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCATGCAGTGGGcc                                                                                        >  4:150604/1‑135 (MQ=255)
                                           aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGGTCCTGCAGTGGGcc                                                                                        >  2:289277/1‑135 (MQ=255)
                                           aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGAGGGAGGGGCGCCGCTTTTTTACTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTTGGc                                                                                         >  5:361826/1‑134 (MQ=255)
                                                            ggctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGCGCCAGTCCGGGGCGGGGGTGCGGTTGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGCCGGCTACCGgcagcc                                                                       >  5:310220/1‑134 (MQ=255)
                                                            ggctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGCGCCAGGCCCGGGCGGGGGGCAGGTAGAAGAACGCGGCGGGGTCCTGCACTGGGCCGACGGCTACCCgccgca                                                                       >  5:119361/1‑135 (MQ=255)
                                                            ggctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGACCGCGGCGGGTTCCTGCAGGGGGCCGACGGCTACCGgcagca                                                                       >  1:263278/1‑135 (MQ=255)
                                                            ggctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCGGGCGGCCGCGCCCGGCCGGGGCGGGGGTCATGTAGAAGAAAGCGGCGGGGTCCTGCAGTGGTACGACGGCTACCGgcagca                                                                       >  7:312294/1‑135 (MQ=255)
                                                             gctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCAc                                                                      >  4:158333/1‑135 (MQ=255)
                                                                  cGGCAGCCACCCGGCGCGCGCGCGGGGGCGGGGCGCCGCTTTGGTCCGGGCGGCCGAGCCAGTCCGGGTCGGGTGTCAGGTACAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGgc                                                                 <  7:184/135‑1 (MQ=255)
                                                                      aGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCgg                                                             >  7:199351/1‑135 (MQ=255)
                                                                           cccggCGCGCGGGGGGGGGCGGGGCGCCGCTGTGGCCCGGGCGGCGGAGCCCGGCGGGGGGGGGGGGCGGTACGACCACCGCGGCGGGTTCCTGCAGGGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGcc                                                        <  2:375244/132‑1 (MQ=255)
                                                                                       gCGTGGGCGGGGCGCCGCTTTTGTACTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGGAGAAGAACCCGGCGGGGTGCTGCCGTGGGCCGCCGGCTACCGGCAGCCCCGGGCGCGGCAGCCCCGCCATGGTGc                                            >  4:11605/1‑135 (MQ=255)
                                                                                            gggggggcgCCGCTTTTGCCCGGGCGGCCGCGCCAGGCCGGGGCGGGGGGCAGGTAGACGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCg                                       <  4:88494/132‑1 (MQ=255)
                                                                                             gcggggcgCCGCTTTTGTCCGGGCGGCCGAGCCAGTCCGGGGCGGGGGGCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGt                                      <  7:133142/135‑1 (MQ=255)
                                                                                             gcggggcgCCGATTTTGTCCTGGCGGCCGAGCCAGGGCGGGGCGGGGGGCAGGTAGAACCACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGt                                      <  8:330052/135‑1 (MQ=255)
                                                                                                 ggcgCGGTTTTTGGCCGGGCGGCCGACCCTGGCGGGGGGGGGGGCCTGGAACACCACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTc                                  <  6:310220/135‑1 (MQ=255)
                                                                                                 ggcgCGGGTTTGGCCCGGGGGGCGGTGCCCGTCGGGGGCGGGGGGCCGGTAGACCACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTc                                  <  3:323035/135‑1 (MQ=255)
                                                                                                 ggcgCCGCTTTTGTCCTGGCGGCCGCGCCCGTCCGGGGCGGGGGGCCGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTc                                  <  5:146131/135‑1 (MQ=255)
                                                                                                    gccgcTTTTGTCCTGGCGGCCGCGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGTGGCCGCCCCCCCGTGGTGCACTCGTGGTCgcg                               >  6:376563/1‑133 (MQ=255)
                                                                                                        cTTTTGCCGGGGGGGCCGAGCCTGCCGGGGGGGGGGGGCTGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACg                           <  5:340334/135‑1 (MQ=255)
                                                                                                           ttGGCCGGGCGGCGGCGCCCGGCGGGGGCGGGGGTCAGGTACACCACCGCGGCGGGGTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGc                         <  1:92068/134‑1 (MQ=255)
                                                                                                                     ggcCGCGCCTGGCCGGGGGGGGGGTCTGGTCGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGa              <  8:258719/135‑1 (MQ=255)
                                                                                                                      gcCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTgcg             >  5:270859/1‑133 (MQ=255)
                                                                                                                      gcCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAg             >  3:232298/1‑135 (MQ=255)
                                                                                                                            ccAGGCCGGGGCGGGGGTATGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCggg       <  4:119895/135‑1 (MQ=255)
                                                                                                                                 ccGGGGCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAgg  <  3:374852/135‑1 (MQ=255)
                                                                                                                                 ccGGGGCGGGGGCCAGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAgg  <  1:289277/135‑1 (MQ=255)
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CCGGGGTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGG  >  KT2440/1720817‑1721080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: