Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,806,731 G→T 52.8% K10N (AAG→AAT)  yeiG → S‑formylglutathione hydrolase YeiG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,806,7310GT52.8% 15.4 / 113.8 76K10N (AAG→AAT) yeiGS‑formylglutathione hydrolase YeiG
Reads supporting (aligned to +/- strand):  ref base G (20/16);  new base T (23/17);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCACCCACACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCT  >  KT2440/1806603‑1806865
                                                                                                                                |                                                                                                                                      
caacaCCCACACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTc                                                                                                                                  >  6:254216/4‑135 (MQ=255)
 atcaCCCACACGATGGGGCTGGAAGACATCAACATGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCtt                                                                                                                                   <  5:201080/133‑1 (MQ=255)
   caCCCACACGATGGGGCTGGATGACATCCACAAGGCCTTCGACGTGATGCATGAAGGCATGAGCATCCGTAGAGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAAGATCTCCTTCCAGAATAGCTTcggc                                                                                                                               <  4:81738/135‑1 (MQ=255)
          acGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGc                                                                                                                        >  8:307811/1‑135 (MQ=255)
          acGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGc                                                                                                                        >  8:155458/1‑135 (MQ=255)
          acGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCAACTTCTGAGGTCAGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGc                                                                                                                        >  3:81738/1‑135 (MQ=255)
                  gCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCg                                                                                                                 >  4:235756/1‑134 (MQ=255)
                       aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCg                                                                                                           <  5:87505/135‑1 (MQ=255)
                       aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCg                                                                                                           >  3:58252/1‑135 (MQ=255)
                        aGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCgg                                                                                                          <  1:210584/135‑1 (MQ=255)
                         gACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGc                                                                                                         >  5:91021/1‑135 (MQ=255)
                               atctaGGGCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGAGCCAGTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCAtt                                                                                                   <  8:151092/131‑1 (MQ=255)
                               aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCg                                                                                                           <  2:354480/127‑1 (MQ=255)
                               aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCg                                                                                                           >  1:354480/1‑127 (MQ=255)
                               aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCAtt                                                                                                   <  4:88202/135‑1 (MQ=255)
                               aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCAtt                                                                                                   >  7:98660/1‑135 (MQ=255)
                                acaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCAtt                                                                                                   <  6:91021/134‑1 (MQ=255)
                                acaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCAtt                                                                                                   <  5:145251/134‑1 (MQ=255)
                                        ttCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGc                                                                                          >  4:391174/1‑135 (MQ=255)
                                         tCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                         >  8:106977/1‑135 (MQ=255)
                                         tCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                         >  5:348985/1‑135 (MQ=255)
                                           gACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                         <  7:106977/133‑1 (MQ=255)
                                            aCCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTggg                                                                                      >  6:278534/1‑135 (MQ=255)
                                                    cATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                         >  4:88273/1‑124 (MQ=255)
                                                    cATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                         <  3:88273/124‑1 (MQ=255)
                                                         aGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                         >  2:309714/1‑119 (MQ=255)
                                                         aGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                         <  1:309714/119‑1 (MQ=255)
                                                         aGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgt                                                                          <  5:238379/134‑1 (MQ=255)
                                                         aGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgt                                                                          <  6:250961/134‑1 (MQ=255)
                                                            cAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTc                                                                      <  7:307811/135‑1 (MQ=255)
                                                              agagCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGtt                                                                       >  4:112517/1‑132 (MQ=255)
                                                              agagCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGtt                                                                       <  3:112517/132‑1 (MQ=255)
                                                                  cATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCtgt                                                                 >  6:61621/1‑134 (MQ=255)
                                                                     ccGTAGCTTGATACACTTATGAGGGCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACTAGCGTTAGCGGCATCATTCCAAGGTGCTGGGTTGCGATATTGTGTTCGCTGTGTAc                                                             <  3:40242/135‑1 (MQ=255)
                                                                        tAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTcggc                                                                                                                               >  5:94084/1‑66 (MQ=255)
                                                                        tAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTcggc                                                                                                                               <  6:94084/66‑1 (MQ=255)
                                                                            gTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCg                                                                                                           <  8:132151/82‑1 (MQ=255)
                                                                            gTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCg                                                                                                           >  7:132151/1‑82 (MQ=255)
                                                                              gATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTgccgcc                                                     >  5:38507/1‑134 (MQ=255)
                                                                                tCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCa                                                  >  4:273061/1‑135 (MQ=255)
                                                                                tCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGAACCTGCCGCCGCa                                                  >  6:154132/1‑135 (MQ=255)
                                                                                  cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAgg                                                >  3:276145/1‑135 (MQ=255)
                                                                                  cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAgg                                                >  7:184580/1‑135 (MQ=255)
                                                                                  cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAgg                                                >  4:295750/1‑135 (MQ=255)
                                                                                  cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAgg                                                >  1:337477/1‑135 (MQ=255)
                                                                                  cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAgg                                                >  3:73479/1‑135 (MQ=255)
                                                                                  cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAgg                                                >  3:255958/1‑135 (MQ=255)
                                                                                  cACTTCTGAGGTCTGTCATGAGCCTAGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAgg                                                >  5:291394/1‑135 (MQ=255)
                                                                                   aCTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGc                                               >  8:338324/1‑135 (MQ=255)
                                                                                     ttCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCg                                             <  7:155458/135‑1 (MQ=255)
                                                                                             tCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAggg                                      >  3:102788/1‑134 (MQ=255)
                                                                                               tGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCg                                    >  2:295101/1‑134 (MQ=255)
                                                                                                  cATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaaaa                                >  5:309871/1‑135 (MQ=255)
                                                                                                  cATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaaaa                                >  2:96796/1‑135 (MQ=255)
                                                                                                  cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGcaca                                                                                                                     >  5:278068/1‑50 (MQ=255)
                                                                                                  cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGcaca                                                                                                                     <  6:278068/50‑1 (MQ=255)
                                                                                                    tGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                              <  8:98660/135‑1 (MQ=255)
                                                                                                    tGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                              <  6:166366/135‑1 (MQ=255)
                                                                                                    tGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                              <  4:58252/135‑1 (MQ=255)
                                                                                                    tGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                              <  4:102788/135‑1 (MQ=255)
                                                                                                    tGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGGTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                              <  5:254216/135‑1 (MQ=255)
                                                                                                         cTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                         <  8:237978/135‑1 (MQ=255)
                                                                                                          tGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCgg                        <  3:295750/135‑1 (MQ=255)
                                                                                                          tGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCgg                        <  3:235756/135‑1 (MQ=255)
                                                                                                              tAACATCTCCTGCCAGAATAGCTTCGGTGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCt                    >  4:187579/1‑135 (MQ=255)
                                                                                                              tAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                         <  2:306015/66‑1 (MQ=255)
                                                                                                              tAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                         >  1:306015/1‑66 (MQ=255)
                                                                                                              tAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCTAGCAGGGCGAAAAGc                              <  1:123021/125‑1 (MQ=255)
                                                                                                              tAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCTAGCAGGGCGAAAAGc                              >  2:123021/1‑125 (MQ=255)
                                                                                                                    ctcCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCAtt                                                                                                   >  3:289380/1‑50 (MQ=255)
                                                                                                                    ctcCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCAtt                                                                                                   <  4:289380/50‑1 (MQ=255)
                                                                                                                    ctcCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTg              >  7:35612/1‑135 (MQ=255)
                                                                                                                     tcCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTgg             >  5:264899/1‑135 (MQ=255)
                                                                                                                      ccTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGc            >  6:341211/1‑135 (MQ=255)
                                                                                                                         gCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCa         >  1:48446/1‑135 (MQ=255)
                                                                                                                           cAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGAACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGc       <  5:154132/135‑1 (MQ=255)
                                                                                                                                gagCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCt  >  1:279813/1‑135 (MQ=255)
                                                                                                                                |                                                                                                                                      
CATCACCCACACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCT  >  KT2440/1806603‑1806865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑