Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,807,474 G→A 19.3% G258D (GGC→GAC)  yeiG → S‑formylglutathione hydrolase YeiG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,807,4740GA19.3% 145.3 / 27.8 62G258D (GGC→GAC) yeiGS‑formylglutathione hydrolase YeiG
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base A (6/6);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

GGCAGGCCAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCACAGCAGCCCCAAT  >  KT2440/1807340‑1807608
                                                                                                                                      |                                                                                                                                      
ggCAGGCCAATGCCCGCCGTTGCTGGGGGATCAGGGCGACCGGGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAgg                                                                                                                                        <  6:255172/135‑1 (MQ=255)
  cAGGCCAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCt                                                                                                                                      >  2:233460/1‑135 (MQ=255)
     gCCAATGCCCGCCGTTGCTGGTGGATCAGTGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGACGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTTCGTATGCAGCCAAGCTATg                                                                                                                                   <  5:143453/135‑1 (MQ=255)
     gCCAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATg                                                                                                                                   <  4:233391/135‑1 (MQ=255)
       cAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGa                                                                                                                                  >  4:84367/1‑134 (MQ=255)
        aaTGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGAcc                                                                                                                                >  1:39843/1‑135 (MQ=255)
         aTGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGAcc                                                                                                                                >  7:140052/1‑134 (MQ=255)
          tGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCAt                                                                                                                              <  1:233460/135‑1 (MQ=255)
          tGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCAt                                                                                                                              <  2:357077/135‑1 (MQ=255)
             ccgccgTTGCTGGTGGATCCGGGCGACCGTGACGACTTCCGCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAg                                                                                                                            <  5:50582/134‑1 (MQ=255)
              cgccgTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGct                                                                                                                          <  1:148600/135‑1 (MQ=255)
                  gTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGctacta                                                                                                                      >  7:352206/1‑135 (MQ=255)
                          tggAGCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCGGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCAt                                                                                                                <  7:349199/133‑1 (MQ=255)
                           ggATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGc                                                                                                             <  3:18985/135‑1 (MQ=255)
                              tCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAg                                                                                                          >  3:194399/1‑135 (MQ=255)
                                   gCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCa                                                                                                     >  7:198774/1‑135 (MQ=255)
                                     gACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCAt                                                                                                    >  5:350671/1‑134 (MQ=255)
                                         gTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCga                                                                                                 >  1:264783/1‑133 (MQ=255)
                                         gTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCga                                                                                                 >  3:107007/1‑133 (MQ=255)
                                          tGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCgagga                                                                                              >  7:269653/1‑135 (MQ=255)
                                          tGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGAGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCgagga                                                                                              >  5:34125/1‑135 (MQ=255)
                                                cTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCt                                                                                        <  5:332657/135‑1 (MQ=255)
                                                cTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCt                                                                                        <  6:105984/135‑1 (MQ=255)
                                                    cTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTAt                                                                                                                                    >  5:115054/1‑87 (MQ=255)
                                                    cTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTAt                                                                                                                                    <  4:106582/87‑1 (MQ=255)
                                                    cTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTAt                                                                                                                                    <  6:115054/87‑1 (MQ=255)
                                                    cTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTAt                                                                                                                                    >  3:106582/1‑87 (MQ=255)
                                                    cTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTgcg                                                                                     >  7:159571/1‑134 (MQ=255)
                                                        agaAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCcatc                                                                                <  6:375283/135‑1 (MQ=255)
                                                                       cTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTTCGCCATCATGCGGTGGCACTgg                                                                 <  4:386453/135‑1 (MQ=255)
                                                                       cTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTgg                                                                 <  2:264783/135‑1 (MQ=255)
                                                                       cTGAAGCGCGGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTgg                                                                 <  3:129261/135‑1 (MQ=255)
                                                                             cgcTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCAt                                                                                                                >  8:373083/1‑82 (MQ=255)
                                                                             cgcTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCAt                                                                                                                <  7:373083/82‑1 (MQ=255)
                                                                             cgcTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCggg                                                           >  2:374676/1‑135 (MQ=255)
                                                                             cgcTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCggg                                                           >  5:376125/1‑135 (MQ=255)
                                                                                  gAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTg                                                                  <  2:50500/123‑1 (MQ=255)
                                                                                  gAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTg                                                                  >  1:50500/1‑123 (MQ=255)
                                                                                  gAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTaa                                                      >  2:125678/1‑135 (MQ=255)
                                                                                   aaCAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAAc                                                     <  6:357233/135‑1 (MQ=255)
                                                                                   aaCAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAAc                                                     >  1:372523/1‑135 (MQ=255)
                                                                                   aaCAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAAc                                                     <  4:255366/135‑1 (MQ=255)
                                                                                   aaCAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTa                                                       <  3:385830/133‑1 (MQ=255)
                                                                                   aaCAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAAc                                                     <  5:19069/135‑1 (MQ=255)
                                                                                           gcACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCtg                                             >  2:259517/1‑135 (MQ=255)
                                                                                           gcACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCtg                                             >  4:151042/1‑135 (MQ=255)
                                                                                             aCGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCtgtg                                           >  6:22668/1‑135 (MQ=255)
                                                                                                    ggTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCgg                                    <  2:206102/135‑1 (MQ=255)
                                                                                                       ggCCATGCAATGACCCTGCGTATGTAGCCAAGCTATGACCATAGCAACTACTTAATTGCCAGCTTCATCGCGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCGTGGGGGAGCGGGCg                                 <  6:34791/135‑1 (MQ=255)
                                                                                                           aTGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATg                                                                             <  8:145188/87‑1 (MQ=255)
                                                                                                           aTGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATg                                                                             >  7:145188/1‑87 (MQ=255)
                                                                                                           aTGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCATCATCTAGGCGCACCTGCGCCATCATCCGCTGGCACTGGGGCGGGTTTAACGCCACCTGTGGGAGAGGGCGTGca                             >  3:143717/1‑134 (MQ=255)
                                                                                                            tGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGccc                            <  1:48120/135‑1 (MQ=255)
                                                                                                            tGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGccc                            <  1:86998/135‑1 (MQ=255)
                                                                                                               aaTGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCcgcg                         <  6:376125/135‑1 (MQ=255)
                                                                                                                  gACCCTGCGTATGCAGCCAAGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAg                      >  2:212617/1‑135 (MQ=255)
                                                                                                                     ccTGCGTATGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGACGGGc                   >  6:248882/1‑135 (MQ=255)
                                                                                                                          gTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGcac              <  8:343670/135‑1 (MQ=255)
                                                                                                                          gTATGCAGCCAGACTATGACCATAGCTACTATTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGcac              <  7:288539/135‑1 (MQ=255)
                                                                                                                           tATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGGTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGACGggcggcgcc             >  5:33952/1‑132 (MQ=255)
                                                                                                                            aTGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCAcag            >  3:205598/1‑135 (MQ=255)
                                                                                                                            aTGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCAcag            >  3:209604/1‑135 (MQ=255)
                                                                                                                            aTGCAGCCAGACTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCAcag            >  4:272020/1‑135 (MQ=255)
                                                                                                                                      gCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCACAGCAGCCCCAAt  >  5:138463/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GGCAGGCCAATGCCCGCCGTTGCTGGTGGATCAGGGCGACCGTGACGACTTCCTCGAGAAGCAACTCAAGCCTGAAGCGCTGGAACAGGCGGCACGCAAAGGTGGCCATGCAATGACCCTGCGTATGCAGCCAGGCTATGACCATAGCTACTACTTCATTGCCAGCTTCATCGAGGAGCACCTGCGCCATCATGCGGTGGCACTGGGGCGGGTTTAACGCCTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCACAGCAGCCCCAAT  >  KT2440/1807340‑1807608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑