| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,927,136 | A→G | 10.8% | D182D (GAT→GAC) | minC ← | Septum site‑determining protein MinC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,927,136 | 0 | A | G | 10.8% | 101.2 / 5.2 | 37 | D182D (GAT→GAC) | minC | Septum site‑determining protein MinC |
| Reads supporting (aligned to +/- strand): ref base A (12/21); new base G (2/2); total (14/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01 | |||||||||||
GCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCTGTTGGCCACCGCGTACTGGCGAAGTAATGATGCGGGTCGGGCGCACCTC > KT2440/1927004‑1927255 | gCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCa < 2:275340/133‑1 (MQ=255) gACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGc < 3:151799/135‑1 (MQ=255) tACTGGCCGGCTATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAg < 5:243310/134‑1 (MQ=255) tACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAg < 4:260260/134‑1 (MQ=255) tACTGGCCGGCGATCGAGACCATTTCGGCGGGCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAg < 6:369094/134‑1 (MQ=255) aCTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGtt < 2:74485/135‑1 (MQ=255) cGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCAc > 8:81483/1‑135 (MQ=255) cGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCAc > 8:337477/1‑135 (MQ=255) gCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCt < 5:182482/135‑1 (MQ=255) tCGAGAGCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCGGTACACATGGCTGTTGCCATCGGCCAGAAGTTCCGCACCTGGGc > 1:350852/1‑135 (MQ=255) tCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCt < 6:150100/131‑1 (MQ=255) tCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCt > 5:150100/1‑131 (MQ=255) ccATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCg < 3:31512/135‑1 (MQ=255) cggcggTCATCTGCTGGCAGAAGATACGCGACTTGCTGATGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATCGCGCCGTACACATGGATGTTGCCATCGGACAGAAGTTCCGCACCTGGGCTGACCGAAGCCg > 5:178434/1‑135 (MQ=255) gTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTcaca > 1:10792/1‑135 (MQ=255) tCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACaa < 1:254631/135‑1 (MQ=255) gctgGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGAt < 4:133425/135‑1 (MQ=255) tgGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGAt < 4:127155/133‑1 (MQ=255) tgGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATc < 5:325929/134‑1 (MQ=255) agaagaTACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCTCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTc < 3:299581/135‑1 (MQ=255) agaagaTACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCAGGGCTGACCGAAGCCGTCACAACCAGATCACCTc < 3:304136/135‑1 (MQ=255) aTACGCGCCTTGGTGATGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGa < 2:253316/135‑1 (MQ=255) cTTGGTGTTGCGTTTGATGCCGGCCAGGGCGCGCCGGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGAt < 6:178434/135‑1 (MQ=255) tGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCt > 2:168205/1‑135 (MQ=255) gtgtTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCTGt < 1:133189/135‑1 (MQ=255) gCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCTGTTGGcc > 7:178362/1‑135 (MQ=255) cGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCTGTTGGCCACCGCGTACTg < 7:348832/135‑1 (MQ=255) gcCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCTGTTGGCCACCGCGTACTGGCGAAGTAatga < 7:208357/135‑1 (MQ=255) cgcgCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCTGTTGGCCACCGCGTACTGGCGAAGTAATGATGc > 1:94157/1‑135 (MQ=255) cgcgCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCGGCGCATAGATCTGTTGGCCACCGCGGACTGGCGCAGTAATGATGc > 3:76436/1‑135 (MQ=255) aTGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCTGTTGGCCACcgcg > 7:9342/1‑110 (MQ=255) aTGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCTGTTGGCCACcgcg < 8:9342/110‑1 (MQ=255) cGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCTGTTGGCCACCGCGTACTGGCGAAGTAATGATGCGGGTCGGGCGCa < 7:337477/135‑1 (MQ=255) cacaTGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCTGTTGGCCACCGCGTACTGGCGAAGTAATGATGCGGGTCGGGCGCACCTc > 8:94137/1‑135 (MQ=255) tGTTGCCGTCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTc < 5:344728/63‑1 (MQ=255) tGTTGCCGTCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTc > 6:338755/1‑63 (MQ=255) tGTTGCCGTCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTc > 6:344728/1‑63 (MQ=255) tGTTGCCGTCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTc < 5:338755/63‑1 (MQ=255) | GCAGACTTTGTACTGGCCGGCGATCGAGACCATTTCGGCGGTCATCTGCTGGCAGAAGATACGCGCCTTGGTGTTGCCTTTGATGCCGGCCAGGGCGCGCCCGCGCATGGCGCCGTACACATGGATGTTGCCATCGGCCAGAAGTTCCGCACCTGGGCTGACCGAAGCCGTCACAACCAGATCACCTCCCTGAGCATAGATCTGTTGGCCACCGCGTACTGGCGAAGTAATGATGCGGGTCGGGCGCACCTC > KT2440/1927004‑1927255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |