Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,997,753 G→A 6.1% T348T (ACC→ACT)  CJ019_01900 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,997,7530GA 6.1% 199.7 / 3.4 65T348T (ACC→ACT) CJ019_01900hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/35);  new base A (2/2);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

TGCAGATTTGCAATAAGCTATTTCTAAATTTGTTAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGATCATTACGAAGCATAGT  >  KT2440/1997623‑1997882
                                                                                                                                  |                                                                                                                                 
tGCAGATTTGCAATAAGCTATTTCTAAATTTGTTAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGtt                                                                                                                                >  1:66067/1‑134 (MQ=255)
        tGCAATAAGCTATTTCTAAATTTGTTAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTg                                                                                                                       >  6:250456/1‑135 (MQ=255)
        tGCAATAAGCTATTTCTAAATTTGTTAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTg                                                                                                                       >  1:394019/1‑135 (MQ=255)
                  tATTTCTAAATTTGTTAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCttttt                                                                                                             >  5:4174/1‑135 (MQ=255)
                       cTAAATTTGTTAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGAt                                                                                                         >  7:90381/1‑134 (MQ=255)
                       cTAAATTTGTTAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGAt                                                                                                         >  1:99430/1‑134 (MQ=255)
                         aaaTTTGTTAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTaa                                                                                                      <  8:289843/135‑1 (MQ=255)
                         aaaTTTGTTAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTaa                                                                                                      <  2:99430/135‑1 (MQ=255)
                           aTTTGTTATCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTTATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAgg                                                                                                    <  6:151474/135‑1 (MQ=255)
                           aTTAGTTAGCTGCGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTaa                                                                                                      <  2:351305/133‑1 (MQ=255)
                             ttGTTAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGAc                                                                                                  >  3:67344/1‑135 (MQ=255)
                                ttAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCGCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACAt                                                                                                <  2:393420/134‑1 (MQ=255)
                                     tGAGGCGATCTGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTgatag                                                                                          <  5:48535/135‑1 (MQ=255)
                                     tGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTgatag                                                                                          <  5:97371/135‑1 (MQ=255)
                                     tGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTgatag                                                                                          <  1:114088/135‑1 (MQ=255)
                                     tGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTgatag                                                                                          <  1:53148/135‑1 (MQ=255)
                                     tGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTgatag                                                                                          <  8:178101/135‑1 (MQ=255)
                                     tGAGGCGATCGGTTTTTTGGATCTGAAACCGCTCGCCTCAGGCTTTGTGATGGGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACTTTgatag                                                                                          <  2:43530/135‑1 (MQ=255)
                                       aGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGa                                                                                                          >  3:106402/1‑117 (MQ=255)
                                       aGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGa                                                                                                          <  4:106402/117‑1 (MQ=255)
                                                  tttttGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTtgt                                                                             <  2:219103/135‑1 (MQ=255)
                                                      tGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGGTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGa                                                                         >  1:124634/1‑135 (MQ=255)
                                                          tgtgAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTcc                                                                     <  2:394019/135‑1 (MQ=255)
                                                          tgtgAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTcc                                                                     <  3:314151/135‑1 (MQ=255)
                                                             gAAACCGCTCGCCTCAGGCTTTGTGTTGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGTTTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGa                                                                   <  5:162339/134‑1 (MQ=255)
                                                              aaaCCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGa                                                                   >  7:150822/1‑133 (MQ=255)
                                                                       gCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTaa                                                        >  2:384892/1‑135 (MQ=255)
                                                                       gCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTaa                                                        >  7:371533/1‑135 (MQ=255)
                                                                          gcatgCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCACTACGGCTTGGTTTTTATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTTCTTTTGCGAGTCCGATAAGGTTGTAATTg                                                     <  4:222096/131‑1 (MQ=255)
                                                                             ggCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTc                                                   <  4:55046/134‑1 (MQ=255)
                                                                              gCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGc                                                 <  5:250456/135‑1 (MQ=255)
                                                                               cTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGc                                                 >  1:258258/1‑134 (MQ=255)
                                                                               cTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGc                                                 >  7:379438/1‑134 (MQ=255)
                                                                                       tgtgTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTAttt                                        <  3:378372/135‑1 (MQ=255)
                                                                                           tATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATttcttc                                    >  1:260366/1‑135 (MQ=255)
                                                                                              ttATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGt                                  <  8:90381/134‑1 (MQ=255)
                                                                                                 tGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCtt                               <  5:17108/134‑1 (MQ=255)
                                                                                                      gtgtTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAgtgt                         >  2:31450/1‑135 (MQ=255)
                                                                                                       tgtTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGtgtg                        >  3:94807/1‑135 (MQ=255)
                                                                                                        gtTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTgg                       >  4:123215/1‑135 (MQ=255)
                                                                                                        gtTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTATTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTgg                       >  3:366324/1‑135 (MQ=255)
                                                                                                          ttaTCTAATCTGTCAATACGGATTGGTTTTAATGGTTCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCTCCAACTATTTCTTCGTCTTCAGTGTGGtt                     <  2:270258/133‑1 (MQ=255)
                                                                                                          tGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTcc                                                                     >  5:3923/1‑87 (MQ=255)
                                                                                                          tGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTcc                                                                     <  6:3923/87‑1 (MQ=255)
                                                                                                          tGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGtt                     <  6:342488/135‑1 (MQ=255)
                                                                                                            aTCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGa                   >  1:308709/1‑135 (MQ=255)
                                                                                                                  gCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGATCAtt              <  5:6337/134‑1 (MQ=255)
                                                                                                                  gCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGATCAtt              <  8:150822/134‑1 (MQ=255)
                                                                                                                    ttGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTgat                                                                                            >  7:147632/1‑54 (MQ=255)
                                                                                                                    ttGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTgat                                                                                            <  8:147632/54‑1 (MQ=255)
                                                                                                                    ttGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGc                                                                           <  5:48888/71‑1 (MQ=255)
                                                                                                                    ttGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGc                                                                           >  6:48888/1‑71 (MQ=255)
                                                                                                                    ttGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGATCATTAc            >  8:149996/1‑134 (MQ=255)
                                                                                                                       cAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGATCATTACGaa         <  1:146246/134‑1 (MQ=255)
                                                                                                                       cAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGATCATTACGaa         <  1:151066/134‑1 (MQ=255)
                                                                                                                       cAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGATCATTACGaa         <  7:378536/134‑1 (MQ=255)
                                                                                                                         aTACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTAttt                                        <  1:375089/101‑1 (MQ=255)
                                                                                                                         aTACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTAttt                                        >  2:375089/1‑101 (MQ=255)
                                                                                                                         aTACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGATCATTACGAAGCa      >  1:362568/1‑135 (MQ=255)
                                                                                                                         aTACGGATTAGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGAt                                                                  <  5:31793/75‑1 (MQ=255)
                                                                                                                         aTACGGATTAGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGAt                                                                  >  6:31793/1‑75 (MQ=255)
                                                                                                                         aTACGGATTAGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGAt                                                                  >  6:26844/1‑75 (MQ=255)
                                                                                                                         aTACGGATTAGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGAt                                                                  <  5:26844/75‑1 (MQ=255)
                                                                                                                          tACGGATTGGTTTTAATGGTGCGATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGATCATTACGAAGCAt     <  5:49198/135‑1 (MQ=255)
                                                                                                                             ggATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGATCATTACGAAGCATAgt  <  2:308709/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                 
TGCAGATTTGCAATAAGCTATTTCTAAATTTGTTAGCTGAGGCGATCGGTTTTTTGGATGTGAAACCGCTCGCCTCAGGCTTTGTGATGTGTATTTATGATAGTGTTGATCTAAGCTTGCAATACGGATTGGTTTTAATGGTGCCATCTTTTTTGATTAAGGACATTGATAGATAAAGTACTTGTGCGAGTCCGATAAGGTTGTAAATGTCGCCAACTATTTCTTCGTCTTCAGTGTGGTTGATCATTACGAAGCATAGT  >  KT2440/1997623‑1997882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑