Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,142,773 G→A 7.7% I268I (ATC→ATT)  rne ← Ribonuclease E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,142,7730GA 7.7% 165.9 / 3.7 52I268I (ATC→ATT) rneRibonuclease E
Reads supporting (aligned to +/- strand):  ref base G (20/28);  new base A (2/2);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

CGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCTTCTTCCTGGGCGTCGATGCTGTCGATCAGTA  >  KT2440/2142646‑2142897
                                                                                                                               |                                                                                                                            
ggCTGCGTTTGGTGGCGCGCGCCGAGTTGATGTCTATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTgg                                                                                                                        <  4:45591/133‑1 (MQ=255)
  cTGCGTTGGGGGGCGCGCGCCGAGTTGATGGCGATCGACACCAGGGCTGCGGGCGGGTCGATCACGATCGAACGGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAg                                                                                                                     <  5:143356/135‑1 (MQ=255)
       tttGGTGGCGCGCGCGGAGTTGATGTCGATCGACACCAGGGCTGCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGtt                                                                                                                <  4:2223/135‑1 (MQ=255)
       tttGGTGGCGCGCGCCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGtt                                                                                                                <  6:201474/135‑1 (MQ=255)
       ttggggggCGCGCGCCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCTAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGtt                                                                                                                <  3:297096/129‑1 (MQ=255)
       ttggggggCGCGCGCCGAGTTGAGGTCGATCGACACCAGGGCTTGGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGtt                                                                                                                <  1:52538/129‑1 (MQ=255)
             ggCGCGCGCCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAg                                                                                                          >  1:69667/1‑135 (MQ=255)
                  gcgcCGAGTTGCGGGCGAGCGACACCAGGGCTTCGGGCGGGGCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTa                                                                                                     <  8:207555/135‑1 (MQ=255)
                  gcgcCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTa                                                                                                     <  2:57302/135‑1 (MQ=255)
                  gcgcCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTa                                                                                                     >  2:228271/1‑135 (MQ=255)
                  gcgcCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTa                                                                                                     <  8:109381/135‑1 (MQ=255)
                  gcgcCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCCGGAAGCGGTTGAACAGCGGTa                                                                                                     >  7:32586/1‑135 (MQ=255)
                  gcgcCGAGTTGATGCCGACCCACACCCGGGCTGCGGCCGGGTCGAGCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTa                                                                                                     <  4:378050/135‑1 (MQ=255)
                  gcgcCGAGTGGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTa                                                                                                     <  7:293867/135‑1 (MQ=255)
                  gcgcCGAGTGCATGTCGCTCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTGTCGATCTGGAAGCGGTTGAACAGCGGTa                                                                                                     <  1:66615/135‑1 (MQ=255)
                    gcCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACg                                                                                                   >  2:46044/1‑135 (MQ=255)
                    gcCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACg                                                                                                   >  1:153021/1‑135 (MQ=255)
                    gcCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACg                                                                                                   >  5:184123/1‑135 (MQ=255)
                             aggcccatcgaCACCAGGGCTGCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTc                                                                                           <  5:302144/128‑1 (MQ=255)
                                     cgaCACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCt                                                                                   >  1:97435/1‑134 (MQ=255)
                                       acacCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGa                                                                                <  3:97859/135‑1 (MQ=255)
                                            cgggCGTCGGGCGGGTCCATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTg                                                                           <  7:339461/134‑1 (MQ=255)
                                            aGGGCTGCGGGCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTg                                                                           <  2:318832/135‑1 (MQ=255)
                                               gCTTCGGGCGGGTCCATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTg                                                                        <  5:301087/135‑1 (MQ=255)
                                                cTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTgg                                                                       >  3:355813/1‑135 (MQ=255)
                                                 ttCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGc                                                                      >  5:30568/1‑135 (MQ=255)
                                                     gactggtcgATAACGATCGAACCGACGGACGGAAGGTCGACCACGCGCTGGAAGGCGGTCTCGAGCTCGCTTACGAACTCGAAGCTGTTGAACAGCTGTACGCAGTCTACGTACAGCATTCCCTTGCAGCCGTAc                                                                  >  4:50282/5‑135 (MQ=255)
                                                      tcgggtcgATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACt                                                                 >  1:71648/1‑135 (MQ=255)
                                                      tcgggtcgATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCAATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTc                                                                                           >  8:16148/1‑109 (MQ=255)
                                                      tcgggtcgATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCAATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTc                                                                                           >  8:22134/1‑109 (MQ=255)
                                                      tcgggtcgATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCAATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTc                                                                                           <  7:16148/109‑1 (MQ=255)
                                                      tcgggtcgATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCAATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTc                                                                                           <  7:22134/109‑1 (MQ=255)
                                                       cgggtcgATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTg                                                                <  1:212229/135‑1 (MQ=255)
                                                                        aaCCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCgg                                                <  4:355813/134‑1 (MQ=255)
                                                                           cgccgGACGGCAGGTCGCCCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGAt                                              >  4:186662/1‑133 (MQ=255)
                                                                             ccgGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAg                                          <  7:265174/135‑1 (MQ=255)
                                                                              cgGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAgg                                         <  1:228271/135‑1 (MQ=255)
                                                                                gACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAgg                                         >  2:36312/1‑133 (MQ=255)
                                                                                           gACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCttcttc                            >  6:150/1‑135 (MQ=255)
                                                                                           gACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCttcttc                            >  7:106294/1‑135 (MQ=255)
                                                                                           gACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCACCACCTGGCGGATGAAGGTCAGCGCttcttc                            >  1:380634/1‑135 (MQ=255)
                                                                                                cgcgCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCTTCTTCCTggg                       >  8:2487/1‑135 (MQ=255)
                                                                                                   gcTAGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCTTCTTCCTGGGCGt                    <  5:11617/135‑1 (MQ=255)
                                                                                                     tGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGCAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCTTCTTCCTGGGCGTCg                  <  4:158747/135‑1 (MQ=255)
                                                                                                        aaGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCTTCTTCCTGGGCGTCGATg               >  6:10818/1‑135 (MQ=255)
                                                                                                             ggTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTGTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCTTCTTCCTGGGCGTCGATGCTGTc          <  7:299860/135‑1 (MQ=255)
                                                                                                             ggTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCTTCTTCCTGGGCGTCGATGCTGTc          <  6:76195/135‑1 (MQ=255)
                                                                                                             ggTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCTTCTTCCTGGGCGTCGATGCTGTc          <  2:69667/135‑1 (MQ=255)
                                                                                                               tctcGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTa                                                                                                     >  6:219338/1‑42 (MQ=255)
                                                                                                               tctcGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTa                                                                                                     <  5:219338/42‑1 (MQ=255)
                                                                                                                  cGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCTTCTTCCTGGGCGTCGATGCTGTCGATCa     >  7:180392/1‑135 (MQ=255)
                                                                                                                          cTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCTTGTTCCTGGGCGTCGATGCTGTCGATCAGTa  <  4:49328/130‑1 (MQ=255)
                                                                                                                               |                                                                                                                            
CGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGACACCAGGGCTTCGGTCGGGTCGATCACGATCGAACCGCCGGACGGCAGGTCGACCACGCGCTGGAAGGCGGTCTCGATCTGGCTTTCGATCTGGAAGCGGTTGAACAGCGGTACGCTGTCTTCGTACAGCTTGACCTTGCTGGCGTACTGCGGCATCACCTGGCGGATGAAGGTCAGCGCTTCTTCCTGGGCGTCGATGCTGTCGATCAGTA  >  KT2440/2142646‑2142897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑