Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,203,886	A→C	35.2%	T74P (ACC→CCC)	CJ019_02088 →	putative protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,203,886	0	A	C	35.2%	49.1 / 7.5	45	T74P (ACC→CCC)	CJ019_02088	putative protein
Reads supporting (aligned to +/- strand):  ref base A (22/6);  new base C (2/15);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 1.43e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGATCAAGGTTGCCCGACATGGCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGTTCCGCAGTGGCCGGAAAGAGCGTGTCAATGTCG  >  KT2440/2203759‑2204015                                                                                                                                |                                                                                                                                  aGTGATCAAGGTTGCCCGACATGGCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACgcc                                                                                                                            >  7:159861/1‑133 (MQ=255) aGTGATCAAGGTTGCCCGACATGGCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGAc                                                                                                                            >  1:217391/1‑135 (MQ=255) aGTGATCAAGGTTGCCCGACATGGCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGAc                                                                                                                            >  2:264854/1‑135 (MQ=255)   tGATCAAGGTTGCCCGACATGGCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTCCCAAGGCTTTCACCACGActc                                                                                                                          >  8:255791/1‑135 (MQ=255)   tGATCAAGGTTGCCCGACATGGCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGActc                                                                                                                          >  8:182362/1‑135 (MQ=255)                  gACATGGCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCCCCACGACTCGGGTGCTGGAAGCaa                                                                                                           >  5:4444/1‑135 (MQ=255)                  gACATGGCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGCCTCTGGTGCTGGAAGCaa                                                                                                           >  5:212253/1‑135 (MQ=255)                  gACATGGCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCaa                                                                                                           >  1:68031/1‑135 (MQ=255)                  gACATGGCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCaa                                                                                                           >  6:307065/1‑135 (MQ=255)                     ttGGCCAGATTGCGTTGGACGCGACCAGCGGCTTTCCCATTCGCGGGGCGGGCGCTGCCATGCGGCAAGCCGGTGTTTGAAACGTGCTCTCGCTTACCAAGGCTTTCCCCACGACTCTGGTGCTGGAAGCAATCg                                                                                                        <  8:94399/134‑1 (MQ=255)                     agggCCAGTTTGCGTTGGCCCCCACCAGCGGTTTATCAAATGGCCGGGCGGGCGCTGCCAGGGGGTAAGACGTTGTTTTAAACGTACCCTCCCTTACCATGGCTTACCCCACGACTCTGGTGCTGGAAGCAAt                                                                                                          <  4:330313/131‑1 (MQ=255)                     aggcccAGTTTGCCTGGGACGCGACCATCGGCTTTTCCAAGCGCCGTGCCGGCGCTGCCATGCGGTACGACGCTGTTTTCAAAGTGCTCTCGCTTCCCACGGCTTTCCCCACGACTCTGGTGCTGGAAGCAATCg                                                                                                        <  4:295645/131‑1 (MQ=255)                     aTGCCCAGATGGCCTTGGACGCGACCATGGGATTTCCCAAGCGCCGTGCGGGGGCTGCCAGGCGGGAAGCCGCTGTTTTAAAAGTTCTCTCGATTCCCACGGCTTCCCCCACGACTCTGGTGCTGGAAGCAATCg                                                                                                        <  4:389855/135‑1 (MQ=255)                       ggCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGAc                                                                                                      >  7:48139/1‑135 (MQ=255)                       ggCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGAc                                                                                                      >  7:11078/1‑135 (MQ=255)                       ggCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGAc                                                                                                      >  6:147988/1‑135 (MQ=255)                         ccATATTGCCTTGGCCGGGACCATGGGCTTTTCAATTCGCGGTGCCGGCGGTGCCTGGCGGGAAGCCGGTGTTTTCAAAGTTCTCTCGCTTACCACGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCt                                                                                                    <  8:48139/135‑1 (MQ=255)                           aGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCg                                                                                                  >  3:330313/1‑135 (MQ=255)                                      gccgccACCATCGGCTTTCCCATGCGCCGTGCCGGCGCGGCCAGGCGGCAACACGCTGTTTACAAAGTTCGCTCGCTAACCACGGCTTTCCCCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTc                                                                                        <  3:336638/132‑1 (MQ=255)                                      gACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTGTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGCCTCTCGTGCTGGAAGAAATCGCCCTCCGCTTATTGTcc                                                                                       >  4:9933/1‑135 (MQ=255)                                         gcgACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTg                                                                                    >  5:117598/1‑135 (MQ=255)                                          cgACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGa                                                                                   >  3:346195/1‑135 (MQ=255)                                              cATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTAc                                                                               >  8:377688/1‑135 (MQ=255)                                              cATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTAc                                                                               >  2:297614/1‑135 (MQ=255)                                                    cTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCATGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAgt                                                                         <  7:349120/135‑1 (MQ=255)                                                    cTTTTCCAAGCGCCGGGCGGGCGCTGCCATGCGGGAAGACGCTGTTTTCAACGTGCTCTCGCTAACCACGGCTTACCCCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAgt                                                                         <  2:16487/135‑1 (MQ=255)                                                                ccGGGCCGGCGCGCCCAGGCGGTAAGACGCTTTTTTCAATGTTCTCTCGATACCCAGGGCTTACCCCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAAt                                                             <  6:368511/135‑1 (MQ=255)                                                                       gggggtGCCATGCGGGAAGCCGGTTTTTTCAAATTTCCCTCGCCTACCAAGGGTTTCCCCACGACTCTGGTGCTTGAAGCAATCGACCTCCGCTTATTGTCCCTGACTACCCGCGGTGGTGATGTAATCCCGGAg                                                      <  6:141704/130‑1 (MQ=255)                                                                              cgaTGCGGGAAGCGGATGTTTTAAATGTTCTCTCGATTACCATGGCTTCCCCCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTggg                                               <  2:217391/133‑1 (MQ=255)                                                                              ccATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACGACCCGAGTTGTGGATGGTATGCCCGAGTTTTggg                                               >  6:247610/1‑135 (MQ=255)                                                                                aTGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCACGGCTTTCCCCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTggggg                                             >  2:308214/1‑135 (MQ=255)                                                                                 ggCGGCAAGACGCTGTTTACAAAGTTCTCTCGCAAACAACGGCTTGCCCCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGgc                                            <  3:388560/134‑1 (MQ=255)                                                                                   cGGGACGACGCGTTTTTCAAAGTGCGCTCGCTTACCACGGCTTACACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGgcgc                                          <  2:103196/135‑1 (MQ=255)                                                                                           cGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGtt                                  >  4:174952/1‑135 (MQ=255)                                                                                                        gTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGGTCCGCAGTGGCCg                      >  4:229415/1‑134 (MQ=255)                                                                                                           gggtcGCGTAACACGGCTAGCCCCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCGCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGTTCCGCAGTGGCCGGaaa                  <  5:311368/132‑1 (MQ=255)                                                                                                           ctctcGCTACCCATGCCTTTCCCCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGTTCCGCAGTGGCCGGaa                   <  8:280404/134‑1 (MQ=255)                                                                                                             ctcGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGCCCTTTTCCCCAGTGGCCCGAAagc                >  2:162325/1‑134 (MQ=255)                                                                                                              acccTTCCCAGGGCTTCCCCCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGTTCCGCAGTGGCCGGAAagag               <  6:212253/132‑1 (MQ=255)                                                                                                                    aCCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGTTCCGCAGTGGCCGGAAAGAGCgtgt          >  7:203316/1‑134 (MQ=255)                                                                                                                      cacggCTTACCCCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGTTCCGCAGTGGCCGGAAAGAGCGTGTCaa       <  6:24173/132‑1 (MQ=255)                                                                                                                          gggttCCCCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGTTCCGCAGTGGCCGGAAAGAGCGTGTCAATGTc   <  1:162325/132‑1 (MQ=255)                                                                                                                          gCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGTTCCGCAGTGGCCGGAAAGAGCGTGTCAATGTc   <  8:203316/135‑1 (MQ=255)                                                                                                                          gCTTCCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGTTCCGCAGTGGCCGGAAAGAGCGTGTCAATGTc   <  1:282462/135‑1 (MQ=255)                                                                                                                           gtttCACCACGACTCTGGTGCTGGAAGCAATCGACCTCTGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGTTCCGCAGTGGCCGGAAAGAGCGTGTCAATGTCg  <  4:33071/134‑1 (MQ=255)                                                                                                                                |                                                                                                                                  AGTGATCAAGGTTGCCCGACATGGCCAGATTGCCTTGGACGCGACCATCGGCTTTTCCAATCGCCGTGCCGGCGCTGCCATGCGGGAAGACGCTGTTTTCAAAGTTCTCTCGCTTACCAAGGCTTTCACCACGACTCTGGTGCTGGAAGCAATCGACCTCGGCTTATTGTCCCTGACTACCCGAGTTGTTGATGTAATCCCGGAGTTTTGGGGGCGCGACCTGTTCCGCAGTGGCCGGAAAGAGCGTGTCAATGTCG  >  KT2440/2203759‑2204015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑