Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,241,556 T→G 24.0% N367T (AAC→ACC)  CJ019_02129 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,241,5560TG24.0% 79.3 / 2.6 44N367T (AAC→ACC) CJ019_02129hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (13/19);  new base G (10/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGGCGCTCAGGCGTTGTTCGCGCTCCACCTGATCGGTGATGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGCACGCCCTCGAAGAACGCATCGAACGATTCATG  >  KT2440/2241427‑2241686
                                                                                                                                 |                                                                                                                                  
gAGGCGCTCAGGCGTTGTTCGCGCTCCACCTGATCGGTGATGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTc                                                                                                                                <  6:245416/134‑1 (MQ=255)
  ggCGCTCAGGCGTTGTTCGCGCTCCACCTGATCGGTGATGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAg                                                                                                                             <  1:304267/135‑1 (MQ=255)
              ttgttCGCGCTCCACCTGATCGGTGATGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTa                                                                                                                 <  6:90769/135‑1 (MQ=255)
                  tCGCGCTCCACCTGATCGGTGATGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGGGCAGTGCCGGTTTCCAGCTGCACCCGGTAGGTg                                                                                                             >  8:18085/1‑135 (MQ=255)
                   cgcgCTCCACCTGATCGGTGATGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCGTTGGATGTAGCGTGCAGTGCCCGTTTACAGCAGCACCCGGCTGGTGa                                                                                                            >  4:336798/1‑135 (MQ=255)
                        tCCACCTGATCGGTGATGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTg                                                                                                        >  8:316014/1‑134 (MQ=255)
                        tCCACCTGATCGGTGATGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCGTTCGCTGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGGAGGTGCTCTg                                                                                                        >  6:150298/1‑134 (MQ=255)
                                   ggTGATGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGGGTTGGGTGTTGCGTGGAGTGGCGGTTTGCAGCTGGACCCGGGAGGGGGTGTGGTTGTTGCcggc                                                                                            >  3:376777/1‑135 (MQ=255)
                                      gttGTCGAGCATGGTGCGCGCCTTGCGTAGGGGGATGCCATGGTCTTGGCGGTACAGCCGCGCGCTGCTTTCGATGTAGCGTGAAGTGCAGTTTTCCAGCTGCACCCGGTAGTTGATCTGATAGTTGCCGTCggg                                                                                         <  2:243102/133‑1 (MQ=255)
                                       aTGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGc                                                                                        >  6:175443/1‑135 (MQ=255)
                                       aTGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCGTTCGATGTTGCGGGGAGTGGCGGTTTGCAGCTGCCCCCGGGAGGGGGTCGGATAGTTGCCGGCGGGc                                                                                        >  3:147417/1‑135 (MQ=255)
                                       aTGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCGTTCGATGTAGCGTGCAGTGGCGGTTTCCCGCTGCACCCGGTAGGTGATCTGATAGTTGCcggccggc                                                                                        >  5:112598/1‑135 (MQ=255)
                                          tCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCCCGCGGCGTTCGAAGTAGCGTTCAGTGCCCGTTTACACCAGCACCCCGGTGGGGAAGGGATAGTTGCCGGCGGGcccg                                                                                     >  7:197972/1‑134 (MQ=255)
                                            gAGCACGGTGCCCGCCATGCGCAGGGGGAGGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTc                                                                                   <  3:267924/135‑1 (MQ=255)
                                            gAGCAAGGTGCCCGCCATGCGAAGGGGGATGCCCTGGTCGGCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTc                                                                                   <  2:336335/135‑1 (MQ=255)
                                             aGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTc                                                                                   <  5:101367/134‑1 (MQ=255)
                                             aGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCGTTGGGTGTTGCGTGAAGTGCCGGTTTGCCGGTGCCCCCGGGTGGGGATCTGGTAGTTTGCGGCGGGgccgtgg                                                                                  >  5:378862/1‑131 (MQ=255)
                                                aaGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTCTTCCAGCTGCACCCGGTAGGTGATCTGATAGGTGCCGGCGGGCCCTTCgcgc                                                                               <  3:89934/135‑1 (MQ=255)
                                                aaGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCGTTAGTTGTTGCGTGGAGTGCCGGTTTACCGCTGCAACCGGTACGTGATGTGATAGTTTGCGGCGGGCCCGTagcgg                                                                               >  4:267924/1‑134 (MQ=255)
                                                  ggTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGCTGTAGCGTGAAGTGCCGGTTTCCAGCTGCACCCGGGAGGTGATCTGATAGTTGCCGGCGGGCCCGTCgcgccg                                                                             >  6:122970/1‑133 (MQ=255)
                                                  ggTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCGTTCGATGTAGCGTGAAGTGGCGTTTTCCAGCGGCCCCCCGGAGGTGTTCTGGTAGTTGCCGGCGGGCCCGTCgcgccg                                                                             >  8:34953/1‑133 (MQ=255)
                                                       ccGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTa                                                                         <  8:188913/134‑1 (MQ=255)
                                                          ccATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGGAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCGACg                                                                       >  4:287452/1‑133 (MQ=255)
                                                                 cAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGCTGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGGAGGCTACGGTAGGCGTg                                                              >  6:207949/1‑135 (MQ=255)
                                                                    ggggATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCg                                                           <  1:286012/135‑1 (MQ=255)
                                                                            ccTGGTCGTCGCGGTACAGCCGCGCGCGGCGTTCGATGTAGCGTGTAGTGCCGGTTTCCCCCGGCCCCCGGGAGGGGATCTGCTAATTTCCGGCGGGCCCGTCGCGCCGGGGACGGGAGGCTTGGAGCATCGGGt                                                   >  7:349892/1‑135 (MQ=255)
                                                                              tGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTg                                                 <  3:125893/135‑1 (MQ=255)
                                                                                           aCAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCg                                    <  2:48607/135‑1 (MQ=255)
                                                                                           aCAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGGTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCg                                    <  2:153846/135‑1 (MQ=255)
                                                                                             aGCCGCGCGCGGCTTTCGATCTAGCGGGACGGGCCGGTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGc                                  <  4:216007/135‑1 (MQ=255)
                                                                                                  cgcgcgGCGTTCGATGTAGCGTGAAGTGCCGTTTTGCAGCTGCACCCGGGAGGTGCTCTGATAGTTGCCGGCGGGCCCGTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGCACGcc                             >  4:165668/1‑135 (MQ=255)
                                                                                                     gcgGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGCACGCCCt                           >  2:216756/1‑134 (MQ=255)
                                                                                                     gcgGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGCACGCCCTc                          >  4:295134/1‑135 (MQ=255)
                                                                                                       ggCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGTGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGCACGCCCTc                          >  1:148291/1‑133 (MQ=255)
                                                                                                                     cGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGTTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGCACGCCCTCGAAGAACGCATCGaa           <  6:378862/134‑1 (MQ=255)
                                                                                                                     cGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGCACGCCCTCGAAGAACGCATCGaa           <  5:171594/134‑1 (MQ=255)
                                                                                                                      gTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCcg                                                                                              >  3:140973/1‑50 (MQ=255)
                                                                                                                      gTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCcg                                                                                              <  4:140973/50‑1 (MQ=255)
                                                                                                                        gAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTc                                                                                   <  3:243559/59‑1 (MQ=255)
                                                                                                                        gAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTc                                                                                   >  4:243559/1‑59 (MQ=255)
                                                                                                                         aaGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGCACGCCCTCGAAGAACGCATCGAACGAtt      <  6:364366/135‑1 (MQ=255)
                                                                                                                           gTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGCACGCCCTCGAAGAACGCATCGAACGATTCa    <  5:300514/135‑1 (MQ=255)
                                                                                                                            tGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGCACGCCCTCGAAGAACGCATCGAACGATTCAt   <  7:5770/135‑1 (MQ=255)
                                                                                                                               cGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGATGCGCTCGTGCTCCGGCACGCCCTCGAAGAACGCATCGAACGATTCATg  >  2:102053/1‑133 (MQ=255)
                                                                                                                                 ttttCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCgcgc                                                                               >  2:120177/1‑54 (MQ=255)
                                                                                                                                 ttttCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCgcgc                                                                               <  1:120177/54‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                  
GAGGCGCTCAGGCGTTGTTCGCGCTCCACCTGATCGGTGATGTCGAGCAAGGTGCCCGCCATGCGCAGGGGGATGCCCTGGTCGTCGCGGTACAGCCGCGCGCGGCTTTCGATGTAGCGTGAAGTGCCGTTTTCCAGCTGCACCCGGTAGGTGATCTGATAGTTGCCGGCGGGCCCTTCGCGCAGGCTACGGTAGGCTTGTCGCATCGAGTTGCGCTCGTGCTCCGGCACGCCCTCGAAGAACGCATCGAACGATTCATG  >  KT2440/2241427‑2241686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑