Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,296,924 C→T 8.6% Q325Q (CAG→CAA)  CJ019_02178 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,296,9240CT 8.6% 174.9 / 6.7 58Q325Q (CAG→CAA) CJ019_02178hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (23/30);  new base T (3/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

CTTCGCGGGTGTTCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGTCGCCAATCTGTACCTGCTGGCTG  >  KT2440/2296791‑2297053
                                                                                                                                     |                                                                                                                                 
gttgggggggGTTCCACCAGGCCACTTCCCGCGCCGGCAACGCCAGCGGGCCTCTGGGGGGCGGCTCCAGGGCCTCGCGCTCTTCGCGGTTGGCGGGCAGGCCCCGTTCGCGGATTTCGTGGCGCAGCAGCGGCt                                                                                                                                  <  8:220880/129‑1 (MQ=255)
cTTCGCGGGTGTTCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCt                                                                                                                                  <  8:119227/135‑1 (MQ=255)
cTTCGCGGGTGTTCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCt                                                                                                                                  <  4:373643/135‑1 (MQ=255)
cTTCGCGGGTGTTCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTGCGTTGCGCAGCAGCGGCt                                                                                                                                  <  2:142205/135‑1 (MQ=255)
cTTCGCGGGGGTTCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGGGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCt                                                                                                                                  <  5:247888/135‑1 (MQ=255)
  tCGCGGGTGTTCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGTTgg                                                                                                                                >  3:280424/1‑135 (MQ=255)
  tCGCGGGTGTTCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTgg                                                                                                                                >  4:296700/1‑135 (MQ=255)
   cgcgGGTGTTCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTgg                                                                                                                                >  8:53736/1‑134 (MQ=255)
   cgcgGGTGTTCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTgg                                                                                                                                >  8:47180/1‑134 (MQ=255)
   cgcgGGTGTTCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTgg                                                                                                                                >  2:323720/1‑134 (MQ=255)
            tCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAAc                                                                                                                      >  6:108418/1‑135 (MQ=255)
              caccaGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACg                                                                                                                     <  1:102179/134‑1 (MQ=255)
                   ggCCACTTCCAGCGCCGGCAACGCCAGCGTGCCCCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTGATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGTTCGTCAGGGTAACGGCGCAg                                                                                                               >  5:87105/1‑135 (MQ=255)
                      cACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGTTGGTCAGGGTAACGGCGCAGGc                                                                                                             >  1:228176/1‑134 (MQ=255)
                       aCTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCg                                                                                                           >  2:141782/1‑135 (MQ=255)
                       aCTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGAGTTCTTTGCGCAGCAGCGGCTGGTCAGTGTAACGGCGCAGGCCg                                                                                                           >  4:268133/1‑135 (MQ=255)
                        cTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGTGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTAGCGCAGCAGCCGCAGGTCACGGTAACCGCGCAGGCCga                                                                                                          >  1:138470/1‑135 (MQ=255)
                           ccAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCATCGCGGGTGGCGGTCATGCCCCGGTCGCTGATTTCGTTGCGCAGCAGCCGCTGGTCACGGTAACGGCGCAGGCCgatga                                                                                                       >  3:319927/1‑135 (MQ=255)
                                                   cGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCa                                                                               <  8:125381/135‑1 (MQ=255)
                                                   cGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCa                                                                               <  4:166093/135‑1 (MQ=255)
                                                   cGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCa                                                                               <  3:280506/135‑1 (MQ=255)
                                                   cGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTAGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCa                                                                               <  2:277213/135‑1 (MQ=255)
                                                      tgtgGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGtt                                                                            >  2:97442/1‑135 (MQ=255)
                                                      tgtgGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGtt                                                                            >  6:369019/1‑135 (MQ=255)
                                                      gggggggCGGCACCAGGGCCTCGCGCTCTTCGCGGGTGGGGGTCATGCCCCGTTGGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGtt                                                                            <  7:151484/132‑1 (MQ=255)
                                                        tgGGTCGGCACCAGGGCCTCGCGCGCTTGGCGGGTGGCGGTCATGCCCCGTTCGCGGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTgg                                                                          <  4:319927/135‑1 (MQ=255)
                                                           gTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGGCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCGGGTCAGGGTACCGGCGCCGGCCGAGGAGCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTg                                                                       >  7:95973/1‑135 (MQ=255)
                                                              ggCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTg                                                                    <  7:13405/135‑1 (MQ=255)
                                                               gCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGTTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTgg                                                                   <  2:228176/135‑1 (MQ=255)
                                                                        ccTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCtt                                                          <  1:323720/135‑1 (MQ=255)
                                                                            gcgcTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGc                                                      <  1:294934/135‑1 (MQ=255)
                                                                            gcgcTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTGCCGCAGCAGCGGCTGGGCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGc                                                      <  7:77475/135‑1 (MQ=255)
                                                                             cgcTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCTGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGc                                                      <  3:160603/134‑1 (MQ=255)
                                                                             cgcTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGcc                                                     >  1:256668/1‑134 (MQ=255)
                                                                             cgcTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCa                                                     >  7:40935/1‑135 (MQ=255)
                                                                              gcTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCAc                                                    <  4:274429/135‑1 (MQ=255)
                                                                              gcTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCAc                                                    <  2:269319/135‑1 (MQ=255)
                                                                              gcTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCAc                                                    <  1:141491/135‑1 (MQ=255)
                                                                                   tCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGCCAAGCCTTCGGCACGCAAg                                               >  3:45107/1‑135 (MQ=255)
                                                                                   tCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAg                                               >  3:165212/1‑135 (MQ=255)
                                                                                     gcgGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGTTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCaa                                                <  1:80578/132‑1 (MQ=255)
                                                                                       ggTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGa                                           >  7:91224/1‑135 (MQ=255)
                                                                                             cGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTgc                                     >  8:385653/1‑135 (MQ=255)
                                                                                                      cccGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGTTGATGTTGCGCGTCAg                              >  4:319210/1‑133 (MQ=255)
                                                                                                      cccGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAg                              >  8:247402/1‑133 (MQ=255)
                                                                                                      cccGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGGTGGGAGCTGCACAAGCCTTCGGCACGCAAGCTGATGTTGCTCGTCAg                              >  2:295298/1‑133 (MQ=255)
                                                                                                        cGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGGCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAg                          <  4:208331/135‑1 (MQ=255)
                                                                                                        cGTGCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAg                          <  5:246799/135‑1 (MQ=255)
                                                                                                         gTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGt                         <  2:125311/135‑1 (MQ=255)
                                                                                                         gTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGt                         <  3:176963/135‑1 (MQ=255)
                                                                                                           tCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGTCg                       <  8:319308/135‑1 (MQ=255)
                                                                                                           tCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGTCg                       <  4:165212/135‑1 (MQ=255)
                                                                                                             gCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGTCGc                      >  3:9070/1‑134 (MQ=255)
                                                                                                              cTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGTCGcc                     <  3:154422/134‑1 (MQ=255)
                                                                                                                        tGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGTCGCCAATCTGTACct          <  4:87420/135‑1 (MQ=255)
                                                                                                                            cagcagCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGTCGCCAATCTGTACctgctg      >  5:190573/1‑135 (MQ=255)
                                                                                                                               cagcGGCTGGTCAGGGTACCGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGTCGCCAATCTGTACCTgctggct   <  8:294278/135‑1 (MQ=255)
                                                                                                                                agcGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGTCGCCAATCTGTACCTgctggctg  <  5:176788/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                 
CTTCGCGGGTGTTCCACCAGGCCACTTCCAGCGCCGGCAACGCCAGCGTGCCGCTGTGGGTCGGCACCAGGGCCTCGCGCTCTTCGCGGTTGGCGGTCATGCCCCGTTCGCTGATTTCGTTGCGCAGCAGCGGCTGGTCAGGGTAACGGCGCAGGCCGATGATCTCCGTAGCCGGCAGCGGCGGCAGTTGGGTGCTGGACAAGCCTTCGGCACGCAAGGTGATGTTGCGCGTCAGCGAGTCGCCAATCTGTACCTGCTGGCTG  >  KT2440/2296791‑2297053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑