Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,350,451 G→A 7.3% intergenic (‑17/+92) CJ019_02223 ← / ← CJ019_02224 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,350,4510GA 7.3% 173.8 / 3.5 55intergenic (‑17/+92)CJ019_02223/CJ019_02224hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base A (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-01

ACTGGTAATCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTCCAGCCAGCGTAACGGCG  >  KT2440/2350320‑2350583
                                                                                                                                   |                                                                                                                                    
aCTGGTAATCGTTCATCTCGGCAACGCCCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGAGGTGGCTGACGGGATTCAGCCATCACAGTTCCTCGGAAagt                                                                                                                                     >  4:146836/1‑133 (MQ=255)
     tAATCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagtggagt                                                                                                                                <  5:70485/133‑1 (MQ=255)
      aaTCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGt                                                                                                                             <  1:60131/135‑1 (MQ=255)
       aTCGTTCATCTCGGCAACGCCCCTGGGGCTCCATTGCTGTTCGATAAGCGCTGCCTAGTGGACCAGGTTCACTGGCAGCGAGCGATGTGGATCACGGGAGTCAGGCATGACAGTTCGTCGGAAAGTCGAGTGGTc                                                                                                                            >  1:384980/1‑135 (MQ=255)
       aTCGTTCATCTCGGCAACGACCCTGGGGCTCTATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTc                                                                                                                            >  1:194331/1‑135 (MQ=255)
       aTCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACTAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagtggagtgg                                                                                                                              >  2:218689/1‑133 (MQ=255)
       aTCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGATGGATGTGGATGAAGTGATTCAGGCATGACAGTTCCTCGGAAagtggagtgg                                                                                                                              >  5:259816/1‑133 (MQ=255)
       aTCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAtgtggagtgg                                                                                                                              >  2:203060/1‑133 (MQ=255)
       aTCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagtggagtgg                                                                                                                              >  8:96209/1‑133 (MQ=255)
       aTCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagtggagtgg                                                                                                                              >  1:19388/1‑133 (MQ=255)
       aTCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagtggagtgg                                                                                                                              >  8:376994/1‑133 (MQ=255)
       aTCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagtggagtgg                                                                                                                              >  4:71830/1‑133 (MQ=255)
       aTCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagtggagtgg                                                                                                                              >  3:154297/1‑133 (MQ=255)
       aTCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagtggagtgg                                                                                                                              >  1:201108/1‑133 (MQ=255)
               tctcGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATc                                                                                                                    <  3:190391/135‑1 (MQ=255)
               tctcGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATc                                                                                                                    >  4:158677/1‑135 (MQ=255)
                     cAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCg                                                                                                              <  3:385676/135‑1 (MQ=255)
                          agccTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGa                                                                                                         <  1:370507/133‑1 (MQ=255)
                          aCCCTGGGGCTCTATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGa                                                                                                         <  2:194331/135‑1 (MQ=255)
                                       aTTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCtt                                                                                              <  6:301498/133‑1 (MQ=255)
                                        ttGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTa                                                                                           <  7:292184/135‑1 (MQ=255)
                                        ttGATGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTTGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTa                                                                                           <  5:312284/135‑1 (MQ=255)
                                              ttCGATTACCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCAGGCCAGTTCCTCGGAAAGTGGAGTCGTCAGCCTATCGCTCCGGGCGCGTCCGGTTCTGCTACGAtac                                                                                     >  8:374575/1‑133 (MQ=255)
                                               tCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCt                                                                                    <  2:264116/135‑1 (MQ=255)
                                                 gATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTg                                                                                   >  7:44479/1‑134 (MQ=255)
                                                 gATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTg                                                                                   >  3:65089/1‑134 (MQ=255)
                                                 gATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAATGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTg                                                                                   >  7:386173/1‑134 (MQ=255)
                                                 gATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAATGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTg                                                                                   >  7:381375/1‑134 (MQ=255)
                                                      gcgcTGCCTTGTGGACGAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGc                                                                              <  1:237545/134‑1 (MQ=255)
                                                      gcgcTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAATGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGc                                                                              <  8:386173/134‑1 (MQ=255)
                                                      gcgcTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAATGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGc                                                                              <  8:381375/134‑1 (MQ=255)
                                                       cgcTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGGCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCt                                                                            <  8:175048/135‑1 (MQ=255)
                                                         cTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagt                                                                                                                                     <  3:273274/76‑1 (MQ=255)
                                                         cTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagt                                                                                                                                     >  4:273274/1‑76 (MQ=255)
                                                             cTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTccc                                                                      <  4:331726/135‑1 (MQ=255)
                                                             cTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTc                                                                        <  4:65089/133‑1 (MQ=255)
                                                              ttGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCa                                                                     >  3:346823/1‑135 (MQ=255)
                                                               tgtgGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCAc                                                                    >  1:85994/1‑135 (MQ=255)
                                                                    acgaGGTTCGCTGGCAGGGAGGGATGTGGTTGTCGGGTTTGAGGCATGACAGGTCCTCGGTTAGTGGAGTGGGCAGCCTTTCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCGACGGGAt                                                               <  2:384980/132‑1 (MQ=255)
                                                                    aCCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGAt                                                               >  6:11697/1‑135 (MQ=255)
                                                                    aCCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGAt                                                               >  7:197849/1‑135 (MQ=255)
                                                                    aCCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGAt                                                               >  6:87893/1‑135 (MQ=255)
                                                                       aGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATAcg                                                            <  6:259816/135‑1 (MQ=255)
                                                                         gTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagt                                                                                                                                     <  6:237349/60‑1 (MQ=255)
                                                                         gTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAagt                                                                                                                                     >  5:237349/1‑60 (MQ=255)
                                                                         gTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGTTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCATCTCCCACGTGATAcgcg                                                          >  4:213666/1‑135 (MQ=255)
                                                                         gTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATAcgcg                                                          >  6:272536/1‑135 (MQ=255)
                                                                         gTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATAcgcg                                                          >  7:180483/1‑135 (MQ=255)
                                                                           tCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCCCGGGATACGCggg                                                        >  6:351877/1‑135 (MQ=255)
                                                                           tCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCggg                                                        >  4:198642/1‑135 (MQ=255)
                                                                                  cAGGGAGGGATGTGGATGCCGGGATTCAGGCATGTCTGTTCCTCGGATAGTGGTGGTGTCAGCCTATGGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCATGCCTGCTCCGACGGGTTACGCGGGCGCCt                                                   <  3:221503/133‑1 (MQ=255)
                                                                                  cAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGc                                                 >  3:65495/1‑135 (MQ=255)
                                                                                            tgtgGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCACACGGGATACGCGGGCGCCTGCGGTAACTCAg                                       >  4:168583/1‑135 (MQ=255)
                                                                                              tgGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAATCCTGCTCCCACGGGATACGCGGGCGCCGGCGGTAACTCcgcg                                     >  8:145635/1‑135 (MQ=255)
                                                                                              tgGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCATATCGCTCCGGGCGAGTGCGGGTCTTGGACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCCGCGGTATCTCAg                                       >  5:240710/1‑133 (MQ=255)
                                                                                                 aTGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGGAACTCAGCGTGa                                  >  8:336941/1‑135 (MQ=255)
                                                                                                       ggATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGCTCCTGCAAGCCTGCTCCCCCGGGATACGCGGGCGCCTGCGGTTACTCAGCGTGGCGCTCg                            >  1:384044/1‑135 (MQ=255)
                                                                                                       ggATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCg                            <  5:11697/135‑1 (MQ=255)
                                                                                                       ggATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCg                            <  5:87893/135‑1 (MQ=255)
                                                                                                       ggATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTGCTTGTACGCTCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGCTAACTCAGCGTGACGCTc                             >  3:370837/1‑134 (MQ=255)
                                                                                                                cATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTc                   >  1:37781/1‑135 (MQ=255)
                                                                                                                 aTGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTcc                  <  2:85994/135‑1 (MQ=255)
                                                                                                                       gTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTccagccag            <  7:195147/135‑1 (MQ=255)
                                                                                                                         tCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTCCAGCCAGCg          <  8:114674/135‑1 (MQ=255)
                                                                                                                         tCCGCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTCCAGCCAGCg          <  4:80731/135‑1 (MQ=255)
                                                                                                                           cTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTCCAGCCAGCGTa        >  7:77412/1‑135 (MQ=255)
                                                                                                                               gAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCGTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTCCAGCCAGCGTAAcgg    <  7:234014/135‑1 (MQ=255)
                                                                                                                               gAAAGTGGAGTGGGCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTCCAGCCAGCGTAAcgg    <  2:115564/135‑1 (MQ=255)
                                                                                                                                 aaGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTCCAGCCAGCGTAAcggcg  <  6:99221/135‑1 (MQ=255)
                                                                                                                                 aaGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTCCAGCCAGCGTAAcggcg  <  2:19388/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                    
ACTGGTAATCGTTCATCTCGGCAACGACCCTGGGGCTCCATTGCTGTTCGATTAGCGCTGCCTTGTGGACCAGGTTCACTGGCAGGGAGGGATGTGGATGACGGGATTCAGGCATGACAGTTCCTCGGAAAGTGGAGTGGTCAGCCTATCGCTCCGGGCGAGTCCGGTTCTTGTACGATCCTGCAAGCCTGCTCCCACGGGATACGCGGGCGCCTGCGGTAACTCAGCGTGACGCTCGCAAACGCTCCAGCCAGCGTAACGGCG  >  KT2440/2350320‑2350583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑