Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | KT2440 | 2,380,000 | T→G | 16.3% | N54T (AAC→ACC) | nrtA ← | Nitrate/nitrite binding protein NrtA |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | KT2440 | 2,380,000 | 0 | T | G | 16.3% | 83.6 / 3.1 | 62 | N54T (AAC→ACC) | nrtA | Nitrate/nitrite binding protein NrtA |
Reads supporting (aligned to +/- strand): ref base T (26/24); new base G (10/2); total (36/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.81e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTGAC > KT2440/2379867‑2380124 | cATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGCGCCCTGGCGCTGGAGGtt > 8:278711/1‑135 (MQ=255) aTGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGGGCGGGAGGt > 7:74296/1‑133 (MQ=255) tGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGa < 8:111719/135‑1 (MQ=255) cGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGACCCCAGGCGCTGGAGGTTGAgg > 6:216015/1‑134 (MQ=255) cAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAgg < 5:372790/134‑1 (MQ=255) aGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGcc > 1:39885/1‑135 (MQ=255) aGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCGGGAGGGTGAGGGTAAGGcc > 7:189519/1‑135 (MQ=255) tACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGtg < 6:288053/135‑1 (MQ=255) gccgccAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCCGCCCCGCCCCCGCTCCCTGGGGCAGGTGGTTGAGGGTAAGGCCGtgctg > 2:130370/1‑135 (MQ=255) gccgccAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGtgct < 7:162811/134‑1 (MQ=255) ccAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCTATGTGCGGGGTGCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTgg < 6:367181/135‑1 (MQ=255) ccAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGGAAGGCCGTGCTGCTgg < 5:378860/135‑1 (MQ=255) ccAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTGCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGGAAGGCCGTGCTGCTgg < 1:103816/135‑1 (MQ=255) gCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCGGGCGCGGGAGGGTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCgggg > 3:263377/1‑135 (MQ=255) cACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGCGCCCGGGCGCTGGAGGGTGAGGGGAAGGCCGTGCTGCTGGGCGAAGCCCGGGGt > 8:235235/1‑135 (MQ=255) cGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGCGCCCTGGGGCGGGCGGTTGAGGGGAAGGCCGTGCTGCAGGGCGCAGCCCTGGGTggcgg > 4:353550/1‑135 (MQ=255) gTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTggcggc < 2:24540/135‑1 (MQ=255) aGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGCGCCCGGGCGCGGGAGGGTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGGGGCGGCGa > 6:299540/1‑135 (MQ=255) aCCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCCGCCCGGCCCACGCGCCCAGGCGCTGGAGGTGGAGGGTAAGGCCGTGCTGCTGGTCGAAGCCCGGGGGGGCGGCGGcc > 6:56974/1‑135 (MQ=255) aCCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCCGCCCCCGAGCCCCGGCGCGGGAGGGTGAGGGTAAGGCCGGGCTCCAGGGCGAACCCCGGGGGTGCGGCGAcc > 6:56298/1‑135 (MQ=255) aGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCGGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCGGGGTGGCGGCGACCACg > 7:128935/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCCGCCCCGCCCCCGCGCCCCGGCGCGGGGGGGTGAGGGGAAGGCCGTGGGGCGGGGCGCAGCCCGGGGGGGCGGCGCCCAccgccca > 1:349461/1‑129 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCCGCCCCGCACACCAGCCCAGGCGCGGGAGGGTGAGGGTAAATCCCGGCGGGAGGGGGCAGCCCAGGGGGGCGGCGCCCCCCAccca > 6:228018/1‑133 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGa > 7:254300/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGAGCCCTGGCGCGGGAGGTTGAGGGTAAGGCCGTGCTGCTTGGCGAATCCCGGGGTGGCGGAGACCACGCCCGa > 6:122649/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGCGGGTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCGGGGTGGCGGCGCCCCCGAcccc > 1:137856/1‑133 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGa > 3:189357/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCAGGCGCGGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGCAGCCCTGGGTGGCGGCGCCCccgaccga > 6:341061/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACCCGCCCGGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCAGGGTGGCGGCGCCCACGACCGa > 4:357426/1‑135 (MQ=255) aTGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCCGCCCGGCCCCCGCGCCCGGGCGCGGGAGGTTGAGGGTTAGGCCGTGCTGCTGGGCGCAGCCCGGGGTGGCGGCGCCCCCGCCCGAGGCGCAGTc > 6:6035/1‑135 (MQ=255) aTGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCGGGAGGGTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGgcgccctc > 6:286670/1‑131 (MQ=255) aTGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCCCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGCCCCCGACCGAGGCGCAGTc > 4:143912/1‑135 (MQ=255) aTGTGCGGCGGCCCGGTCCCCGCTGACCAGCTTGGCGCGCAGCCCGGCCCACGAGCCCGGGCGCTGGAGGTGGAGGGTCCGGCCGGGCTGCGGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTc < 5:228018/135‑1 (MQ=255) gcggcgTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGt < 2:40659/134‑1 (MQ=255) gcggcgTCCAGTTCGCCGCTGACCAGCTTGGCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCGGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGt < 5:11545/134‑1 (MQ=255) gcggcgTCCAGTGCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGt < 3:242188/134‑1 (MQ=255) gcagcgTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGt < 6:10636/131‑1 (MQ=255) ggcgTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACCCGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCGCGACCGAGGCGCAGTCGGGCAg > 3:114303/1‑135 (MQ=255) cgTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGCGCCCTGGCGCGGGAGGGTGAGGGTTAGGCCGTGCGGCTGGGCGAAGCCCGGGGTGGCGGCGCCCACGACCGCGGCGCCGTCGGTCAggg > 7:196922/1‑135 (MQ=255) tCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGcc > 2:269435/1‑135 (MQ=255) cctgTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGGTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCa < 1:177773/132‑1 (MQ=255) ccAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGGGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCa < 8:17016/135‑1 (MQ=255) tCGCCGCGGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGGGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAg < 2:349461/135‑1 (MQ=255) tGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGCGGCGCAGTCGGTCCGGGCCATATAGCCGATGt > 3:259675/1‑135 (MQ=255) ccAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCa < 4:355412/135‑1 (MQ=255) gcAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGtttttt > 5:85689/1‑135 (MQ=255) gcAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGtttttt > 5:80131/1‑135 (MQ=255) gCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGGAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCg < 8:390055/135‑1 (MQ=255) ggCCCACGCGCCCGGGCGCTGGAGGTGGAGGGGAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCAGATAGCCGATGTTCAGGCTGGTTTTTTCCGgcgc > 3:94707/1‑135 (MQ=255) cccACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGt < 3:136609/135‑1 (MQ=255) cccACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGt > 8:224247/1‑135 (MQ=255) aGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCGGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGc < 5:128303/135‑1 (MQ=255) cccTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCg > 8:101624/1‑135 (MQ=255) ttgcgcTGGTGGGTGCGGGTAAGGCCGTGCTGCTGGGCGATGCCCTGGGTGGCGGCGACCACGACAGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTg < 6:224685/133‑1 (MQ=255) tGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTg < 3:353550/135‑1 (MQ=255) tGGCGCTGGAGGGTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTg < 2:230559/135‑1 (MQ=255) gcgcTTGAGGTTGAGGGTAAGGCCGTTCTGCTGGGCGAACCCCTGGGTGGCGGCGACCACCACCGACGCGCATTCGGTCAGGGCCATATAGCCGATGTACACGCTGGTTTTTTCCCGCGCGTCGCTGGCGTTGAc > 4:211371/1‑135 (MQ=255) gcgcTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGgcgc < 3:61594/71‑1 (MQ=255) gcgcTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGgcgc > 4:61594/1‑71 (MQ=255) gcgcTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTGAc > 6:144125/1‑135 (MQ=255) cgcTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTg < 2:55887/48‑1 (MQ=255) cgcTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTg > 1:55887/1‑48 (MQ=255) cTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGt > 7:153117/1‑75 (MQ=255) cTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGt < 8:153117/75‑1 (MQ=255) | CATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTGAC > KT2440/2379867‑2380124 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |