Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,712,958 A→C 19.3% T83P (ACC→CCC)  CJ019_02561 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,712,9580AC19.3% 89.0 / 4.0 53T83P (ACC→CCC) CJ019_02561hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (8/33);  new base C (10/2);  total (18/35)
Fisher's exact test for biased strand distribution p-value = 1.02e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGAAATCCTCGGGCGCAAGTTCAGCCTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAGCGCGACACGCCCGAGGTGCTCGATGCCTACGTCAAGG  >  KT2440/2712832‑2713087
                                                                                                                              |                                                                                                                                 
aaCGAAATCCTCGTGCGCAAGTTCAGCCTCAAAGCCCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCCGTGCCCGGCCGTCGTCCCGCCCAcccat                                                                                                                           >  1:278490/1‑131 (MQ=255)
    aaaTCCTCGGGCGCAAGTTCAGCCTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCCGTGCCCGGCCGTCTGCCCCCCCCCGCAGgcgc                                                                                                                       >  6:304766/1‑135 (MQ=255)
    aaaTCCTCGGGCGCAAGTTCAGCCTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGgcgc                                                                                                                       <  7:321728/135‑1 (MQ=255)
    aaaTCCTCGGGCGCAAGTTCAGCCTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGgcgc                                                                                                                       >  7:176989/1‑135 (MQ=255)
     aaTCCTCGGGCGCAAGTTCAGCCTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGCCCCCCCTGGcgcg                                                                                                                      >  6:111444/1‑135 (MQ=255)
               cgcAAGTTCAGCCTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGa                                                                                                                                   <  8:295926/112‑1 (MQ=255)
               cgcAAGTTCAGCCTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGa                                                                                                                                   >  7:295926/1‑112 (MQ=255)
                   aggtCAGCCTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTc                                                                                                        <  7:40944/132‑1 (MQ=255)
                     ttCAGCCTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGc                                                                                                      <  1:285600/135‑1 (MQ=255)
                      tCAGCCTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGCCCACGCTGGCGCGGGCGGCGCAGCTTCg                                                                                                       >  4:148014/1‑133 (MQ=255)
                          ccTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAgct                                                                                                 <  4:314710/135‑1 (MQ=255)
                                   cccGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGGTGAgggg                                                                                        <  3:149906/135‑1 (MQ=255)
                                       cAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTGGGGCCCCCCCCGCCAGGGCGGGGGGGGGCGCTTCGCAACCAGCGGAGGGGgcgc                                                                                    >  7:203817/1‑135 (MQ=255)
                                        taGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGccgcg                                                                                   <  7:107135/134‑1 (MQ=255)
                                        aaGGTACCGTGCGCACCCTGTCGCGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGGGCCCGGCCGTCTGCCCGACCACGCTGGGGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGccgcg                                                                                   <  1:219482/135‑1 (MQ=255)
                                            tAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGC‑GGCGGCGCAGATTCGCAAGCCGCTGAGGGGccgcgaccgc                                                                              <  5:338261/135‑1 (MQ=255)
                                             aaCGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGccgcgaccgc                                                                              <  2:143034/135‑1 (MQ=255)
                                             aaCGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGccgcgaccgc                                                                              <  4:296298/135‑1 (MQ=255)
                                             aaCGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTGCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGccgcgaccgc                                                                              <  3:84084/135‑1 (MQ=255)
                                             aaCGTGCGCACCCTGGCGAGCTTTTACGGCAGCAGGCCGATGGGCTTCTGCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGccgcgaccgc                                                                              <  8:86726/135‑1 (MQ=255)
                                             aaCGTGCGCACCCTGGCGAGATTTTACGGCTGCATGCGGATGGTCTTCTTCGGCTTCACCCTGTGCCCGGCGGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTGGCAAGCTGCTGAGGGGccgcgaccgc                                                                              <  7:165298/135‑1 (MQ=255)
                                                       cccTGTCGAGCTTTTACGGCAGAATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCGGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTcc                                                                    <  8:146357/135‑1 (MQ=255)
                                                         cTGTCTAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCTCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAg                                                                  <  3:64409/135‑1 (MQ=255)
                                                         cTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAg                                                                  <  5:102576/135‑1 (MQ=255)
                                                         cTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAg                                                                  <  1:257344/135‑1 (MQ=255)
                                                         cTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAg                                                                  <  3:238026/135‑1 (MQ=255)
                                                          tGTCGAGCTTTTACGGCAGCATGCCGATGGGCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAgg                                                                 <  5:203304/135‑1 (MQ=255)
                                                             cGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGCCCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGCCCGCGACCAGTTCCAggtgg                                                              >  4:128181/1‑135 (MQ=255)
                                                             cGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCCCGCTGGCGCGGGCGGCGCAGATTAGCAAGCTGCTTAGGGGCCGCGCCCCCGCCCTGTTGCAggtgg                                                              >  4:64409/1‑135 (MQ=255)
                                                             cGAGATTTTACTGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCAGCCCCACCACGCTGGCGCCGGCCGCGCACATTACCAAGCTGCAGAGGGGGCGCGCCCCCCACCAGTTGCAagtgg                                                              >  4:357461/1‑135 (MQ=255)
                                                              gAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGCCCCCCCAGGCGCGGGCGCCGCAACATAGCAACCTGCTGACGGGCCCCGACCCCCCCCAGTTCCAgggggt                                                             >  8:346242/1‑135 (MQ=255)
                                                              gAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGAATCGCAAGCTGCTGAGGGGCCGCGCCCGCGACCTGTTACAggtggt                                                             >  1:9394/1‑135 (MQ=255)
                                                               aGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGGCCGCCCACCCAGGCGCGGGCGGCGGAGATTAACAAGCTGGAGACGGGCCCCGGCCCCGCCCACTTCCAAggggtg                                                            >  1:314448/1‑135 (MQ=255)
                                                               aGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGCCCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGCCCGCGACCTGTTCCAGgtggtg                                                            >  3:173310/1‑135 (MQ=255)
                                                                gCTTTTACGGCAGCTTGCGGATGGTCTTCTTGGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCAGGCTGGGGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGgtgt                                                           <  4:369866/135‑1 (MQ=255)
                                                                 cTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCGGCCCGCCCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGtt                                                          <  1:86813/135‑1 (MQ=255)
                                                                       cGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTTCCCGACCACCCTCGCGCGGGCGGCGCAGTATAGCAAGCATCTGAGGGGCCGCGCCCGCGCCACGTTCCAGGGTGTGTTAATCAc                                                    >  6:351898/1‑135 (MQ=255)
                                                                       cGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCGGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTtcatca                                                     <  8:176989/134‑1 (MQ=255)
                                                                       cGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCCACCACGCATGGGCGGGCGGCGCCGTTTTGCCAGCGTCTGAGGGGCCGCGACCGCGACCTGTTACAGGGGGTGTTGATGAc                                                    >  3:232729/1‑135 (MQ=255)
                                                                                      ggTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCTGACCACGCTGGCTCGGGCGGCGCATATTCGCAAGCTTCTGTGGGGCCGCGAGCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAgcg                                     <  4:63445/135‑1 (MQ=255)
                                                                                      ggTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAgcg                                     <  3:169537/135‑1 (MQ=255)
                                                                                      ggTCTTCTTCGGCTGCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAgcg                                     <  2:278490/135‑1 (MQ=255)
                                                                                       ggcttcttCGGCTTCACCCCGTGCCCGGCCGTCTCCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCGGCGGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAgcg                                     <  2:359959/132‑1 (MQ=255)
                                                                                       gTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAgcg                                     <  4:168383/134‑1 (MQ=255)
                                                                                           tcttcGGCTCCACCCCGTGCCCGGCCGTCTGCCCGACCCCGCTGGCGCGGGCGGCGCATATGCGCATGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAGCGCGaca                                <  8:203817/135‑1 (MQ=255)
                                                                                             ttgcgCTTCCCCCCGGGCCCGGCGGACGGCCCGCCCAGGCTGGCGCGGGCGGCGCAGATTCGCAAGCGGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAGCGCGACACg                              <  7:190723/131‑1 (MQ=255)
                                                                                                ggCTACCCCCAGCGCCGGGCCGGCTGCCCGACCACGCTGGCGCGGGCGGCGCATTTGCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGGTCCAGGTGGTGTGCAACACCCTGGACCCGGAGCGCGACACGccc                           <  2:110617/135‑1 (MQ=255)
                                                                                                 gCTTCACCCCGTGCCCGGCCGTCTGCCCGACCACGCTGGGGCGGGCGGCGCAGATTCGCCAGCTGCTGCGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAGCGCGACACGccc                           <  4:107857/134‑1 (MQ=255)
                                                                                                    tCACCCAGTGCCCGGCCGTCAGCCCCACCACCCTGGCGCGGGCGGCGCACAATCGCAACCTGCTGAGGGGCCCCGACCCCGACCTGTTACAGGTGGTGTTCATAACCCTGGACCCGGAGCGCCCCACGCCCGAgg                       >  4:194778/1‑135 (MQ=255)
                                                                                                     cACCCAGTGCCCGGCCGTCTGCCCGCCCACGCTGGCGCGGGCCGCGCCGAATCGCCAGCAGCTGAGGGGCCGCGACCGCGCCCTGTTGCAGGTGGGGGTAAGCCCCCTGGACCCGGAGCGCCCCACGCCCCAGGt                      >  2:27695/1‑135 (MQ=255)
                                                                                                      aCCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAGCGCGACACGCCCGAGGt                      <  8:261740/134‑1 (MQ=255)
                                                                                                            tGCCGGGCGGGCTGCCCGACCCCGGTGGCGCGGGCGGCGCTGATTCCCATGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAGCGCGACACGCCCGAGGTGCTCGAt               <  5:304766/135‑1 (MQ=255)
                                                                                                                    cGTCTGCCCGACCACGCGGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAGCGCGACACGCCCGAGGTGCTCGATGCCTACGt       <  8:215739/135‑1 (MQ=255)
                                                                                                                    cGTCTGCCCGACCACGCGGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAGCGCGACACGCCCGAGGTGCTCGATGCCTACGt       <  7:224497/135‑1 (MQ=255)
                                                                                                                         gCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGCCCTGTTCCAGGTGGTGTTCATCACCCTGGCCCCGGAGCGCGCCACGCCCGAGGTGCTCGCTGCCTACGTCAAgg  >  8:136647/1‑135 (MQ=255)
                                                                                                                              |                                                                                                                                 
AACGAAATCCTCGGGCGCAAGTTCAGCCTCAAAGACCCGCAAGGTAACGTGCGCACCCTGTCGAGCTTTTACGGCAGCATGCCGATGGTCTTCTTCGGCTTCACCCAGTGCCCGGCCGTCTGCCCGACCACGCTGGCGCGGGCGGCGCAGATTCGCAAGCTGCTGAGGGGCCGCGACCGCGACCTGTTCCAGGTGGTGTTCATCACCCTGGACCCGGAGCGCGACACGCCCGAGGTGCTCGATGCCTACGTCAAGG  >  KT2440/2712832‑2713087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑