Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,829,804 A→G 6.2% L101P (CTG→CCG)  gabD1 ← Succinate‑semialdehyde dehydrogenase [NADP( )]1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,829,8040AG 6.2% 203.5 / 3.2 64L101P (CTG→CCG) gabD1Succinate‑semialdehyde dehydrogenase [NADP( )]1
Reads supporting (aligned to +/- strand):  ref base A (26/34);  new base G (2/2);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

GAGCGAGGATGCGTGCTACTTGGTAGTAGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTGGCCTGGGCGCGTAGCAGC  >  KT2440/2829670‑2829938
                                                                                                                                      |                                                                                                                                      
gAGCGAGGATGCGTGCTACTAGGTAGTAGGGGAATTTCCATGGATCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTagca                                                                                                                                        <  7:354401/135‑1 (MQ=255)
  gCGAGGATGCGTGCTACTTGGTAGTAGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTagcagc                                                                                                                                      <  5:226124/135‑1 (MQ=255)
    gAGGATGCGTGCTACTTGGTAGTAGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGccc                                                                                                                                    >  8:38182/1‑135 (MQ=255)
    gAGGATGCGTGCTACTTGGTAGTAGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGccc                                                                                                                                    >  6:376607/1‑135 (MQ=255)
      ggATGCGTGCTACTTGGTAGTAGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCt                                                                                                                                  <  1:260936/135‑1 (MQ=255)
            gTGCTACTTGGTAGTAGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCAt                                                                                                                            <  5:193893/135‑1 (MQ=255)
            gTGCTACTTGGTAGTAGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCAt                                                                                                                            <  2:83681/135‑1 (MQ=255)
            gTGCTACTTGGTAGTAGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCAt                                                                                                                            <  1:59945/135‑1 (MQ=255)
            gTGCTACTTGGTAGTAGGGGAAGTTCCATGGATCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCGCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCAt                                                                                                                            <  5:140224/135‑1 (MQ=255)
            gTGCTACTTGGTAGTAGGGGAAGGTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCAt                                                                                                                            <  1:360597/135‑1 (MQ=255)
                  cTTGGTAGTAGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCGGCCCCTCCGCATGCTTAg                                                                                                                      >  7:150588/1‑135 (MQ=255)
                  cTTGGTAGTAGGGGAACTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGATGCCTCTCCCTAGGACGGGTCTGAAACACGGAGTTTCTCTGGAGCTAGCGGCCCCTCCGCCTGCATAg                                                                                                                      >  7:150591/1‑135 (MQ=255)
                      gtagtaGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCg                                                                                                                    <  3:214630/133‑1 (MQ=255)
                      gtagtaGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCg                                                                                                                    <  6:80111/133‑1 (MQ=255)
                      ggagtagGGGAAGTTGCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTTAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCg                                                                                                                    <  1:289454/131‑1 (MQ=255)
                        agtagGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGGCGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCGCCGCAGGCTTAGCGTGAt                                                                                                                >  5:241112/1‑135 (MQ=255)
                          tagGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATAc                                                                                                              >  3:182914/1‑135 (MQ=255)
                             gggAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATActc                                                                                                            >  3:13384/1‑134 (MQ=255)
                                    tCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTc                                                                                                    >  5:324679/1‑135 (MQ=255)
                                    tCCATGGTTCGATGGCTAGAATGATTCCCAGAGGCTCGTGATTTAAAGTTGACTCTCACTCGGACGGTTCTGCAACAGCGAGTTACTCTGGAGATAGCAGCCACACCGCATGCTTAGCGTAATAATCTAAAATTc                                                                                                    >  8:183556/1‑135 (MQ=255)
                                           ttCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTg                                                                                              >  2:59261/1‑134 (MQ=255)
                                                tGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAAtct                                                                                        >  7:19836/1‑135 (MQ=255)
                                                        aTGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCACCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCg                                                                                >  1:88748/1‑135 (MQ=255)
                                                          gATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGcc                                                                              <  8:19836/135‑1 (MQ=255)
                                                          gATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGcc                                                                              <  1:19395/135‑1 (MQ=255)
                                                               ccAGCTGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAg                                                                         <  4:13384/135‑1 (MQ=255)
                                                               ccAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAg                                                                         >  8:254490/1‑135 (MQ=255)
                                                                cAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAg                                                                         <  5:198750/134‑1 (MQ=255)
                                                                 aGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCt                                                                       >  4:37010/1‑135 (MQ=255)
                                                                    ggCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCa                                                                    >  7:33619/1‑135 (MQ=255)
                                                                    ggCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCa                                                                    <  3:133330/135‑1 (MQ=255)
                                                                    ggCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCa                                                                    >  6:163699/1‑135 (MQ=255)
                                                                     gCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAg                                                                   <  2:127670/135‑1 (MQ=255)
                                                                         gTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCgg                                                                >  7:139174/1‑134 (MQ=255)
                                                                         gTGAGTTAAAGTTGCCTCTCCCTCGGACGGGTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAAATAGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGt                                                               >  3:243456/1‑135 (MQ=255)
                                                                               tAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCtt                                                         <  8:116109/135‑1 (MQ=255)
                                                                               tAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCtt                                                         <  7:315064/135‑1 (MQ=255)
                                                                               tAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCtt                                                         <  3:144758/135‑1 (MQ=255)
                                                                                        ctctCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCAtct                                                 <  1:155179/134‑1 (MQ=255)
                                                                                         tctcCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATctct                                               <  2:236280/135‑1 (MQ=255)
                                                                                              cTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAgg                                           >  6:314111/1‑134 (MQ=255)
                                                                                              cTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAg                                            <  7:288117/133‑1 (MQ=255)
                                                                                              cTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGACCCTCCGCATGCTTAGCGTAATACTCTAATATTCGCGCTGAAATCTCGACTTCGCCCACAGCTTCAGCTGTGATCTTGCCCATCTCTAAgga                                          >  4:87380/1‑134 (MQ=255)
                                                                                                          tGCAACAGGGATTTTCTCTGGAGCTAGCGGCCACTCCGCATGCTTAGGGTAATACTCTAATATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCACATCTCTAAGGTTTGCAGGTggg                               <  8:150591/134‑1 (MQ=255)
                                                                                                          tGCAACAGGGAGTTTCTCTGGAGCTAGCGGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTggg                               <  8:150588/134‑1 (MQ=255)
                                                                                                          tGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCtt                                                                      <  6:292739/95‑1 (MQ=255)
                                                                                                          tGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCtt                                                                      >  5:292739/1‑95 (MQ=255)
                                                                                                          tGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTggg                               <  4:161484/134‑1 (MQ=255)
                                                                                                          tGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTggg                               <  3:37010/134‑1 (MQ=255)
                                                                                                          tGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTggg                               <  7:175021/134‑1 (MQ=255)
                                                                                                          tGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTggg                               <  2:42345/134‑1 (MQ=255)
                                                                                                          tGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCAAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGc                              >  7:154816/1‑135 (MQ=255)
                                                                                                               cAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGt                         <  7:132031/135‑1 (MQ=255)
                                                                                                               cAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGt                         <  1:104282/135‑1 (MQ=255)
                                                                                                                    aGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTg                    >  1:358009/1‑135 (MQ=255)
                                                                                                                    aGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTg                    >  3:317744/1‑135 (MQ=255)
                                                                                                                    aGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGAGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTg                    >  5:6772/1‑135 (MQ=255)
                                                                                                                     gTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTgg                   <  5:238831/135‑1 (MQ=255)
                                                                                                                     gTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTgg                   <  5:376607/135‑1 (MQ=255)
                                                                                                                       ttCTCTGGAGCTAGCAGCCCCTCCGCATGCATAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTGGcc                 >  8:21254/1‑135 (MQ=255)
                                                                                                                            tGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTGGCCTGGgc            >  3:243084/1‑135 (MQ=255)
                                                                                                                             ggAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTGGCCTgg              <  2:5673/132‑1 (MQ=255)
                                                                                                                              gAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTGGCCTGGgcgc          <  7:315551/135‑1 (MQ=255)
                                                                                                                                 cTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTGGCCTGGGCGCGTa       >  5:194145/1‑135 (MQ=255)
                                                                                                                                  tAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTGGCCTGGGCGCGTag      <  2:88748/135‑1 (MQ=255)
                                                                                                                                  tAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCGGACCTCTCGACTTCGCCCAACGCTTCAGCGGTGATCTTGCCCAGCTCTAAGGTTAGCAGGGGGGCATAGTCGCTGGCCTGGGCGCGTag      <  5:324518/135‑1 (MQ=255)
                                                                                                                                      agcCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCGGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGAAGGTGGGCATATTCGCTGGCCTGGGCGCGTagcagc  >  1:381010/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GAGCGAGGATGCGTGCTACTTGGTAGTAGGGGAAGTTCCATGGTTCGATGGCTAGAATGATTCCCAGCGGCTCGTGAGTTAAAGTTGCCTCTCCCTCGGACGGTTCTGCAACAGGGAGTTTCTCTGGAGCTAGCAGCCCCTCCGCATGCTTAGCGTAATACTCTAAAATTCGCGCTGAAATCTCGACTTCGCCCAAAGCTTCAGCGGTGATCTTGCCCATCTCTAAGGTTAGCAGGTGGGCATAGTCGCTGGCCTGGGCGCGTAGCAGC  >  KT2440/2829670‑2829938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑