Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,244,200	G→A	8.3%	A176A (GCG→GCA)	CJ019_03044 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,244,200	0	G	A	8.3%	147.6 / 2.8	48	A176A (GCG→GCA)	CJ019_03044	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/24);  new base A (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCGGGGTTTGTCGTGGTCAGCGGCTTGCTGCTGGCGGCGGGCTTTGCAGTCGGTTGCCTGCTGCGCCGCCACAGCGGCCTGCAGAAGGCCTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTGGGCGGTCGAGGCCGGCTGGGTT  >  KT2440/3244078‑3244331                                                                                                                            |                                                                                                                                    aGCGCGGGGTTTGTCGTGGTCAGCGGCTTGCTGCTGGCGGCGGGCTTTGCAGTCGGTTGCCTGCTGCGCCGCCACAGCGGCCTGCAGAAGGCCTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGcc                                                                                                                         >  1:347155/1‑135 (MQ=255) aGCGCGGGGTTTGTCGTGGTCAGCGGCTTGCTGCTGGCGGCGGGCTTTGCAGTCGGTTGCCTGCTGAGCCGCCACAGCGGCCTGCAGAAGGCCTTTGGCGGTGGCCTGTAGT‑CCGGCGCCGCGCTGGAACTGGcc                                                                                                                         >  2:102469/1‑135 (MQ=255)     cgGGGTTTGTCGTGGTCAGCGGCTTGCTGCTGGCGGCGGGCTTTGCAGTCGGTTGCCTGCTGCCCCGCCACAGCGGCCTGCAGAAGGCCTATGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTTGCCGGt                                                                                                                      >  8:359729/1‑134 (MQ=255)           ttGTCGTGGTCAGCGGCTTGCTGCTGGCGGCGGGCTTTGCAGTCGGTTGCCTTCTGCGCCGCCCCAGCGGCCTGCAGAAGGCCTTTGGCGGTGGCCTGTTGT‑CCGGCGCCGCGCTGGTACTGGCCGGTtgatga                                                                                                                >  4:348180/1‑134 (MQ=255)                gTGGTCAGCGGCTTGCTGCTGGCGGCGGGCTTTGCAGTCGGTTGCCTGCTGCGCCGCCACAGCGGCCTGCAGAAGGCCTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCACTGGTACTGGCCGGTTGATGAACAGCg                                                                                                          >  3:245958/1‑135 (MQ=255)                     cAGCGGCTTGCTGCTGGCGGCGGGCTTTGCAGTCGGTTGCCTGCTGCGCCGCCACAGCGGCCTGCAGAAGGCCTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTg                                                                                                     >  4:152712/1‑135 (MQ=255)                      aGCGGCTTGCTGCTGGCGGCGGGCTTTGCAGTCGGTTGCCTGCTGCGCCGCCACAGCGGCCTGCAGAAGGCCTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTgg                                                                                                    >  2:266967/1‑135 (MQ=255)                                       ggcggGGTTTGCTGGGGGTTGCGGGCTGCGCCGCCACTGGGGCCGGCAGAGGGCGTTGGGCGGTGGCGTGTTGG‑CGGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCctgctg                                                                                   <  5:371954/135‑1 (MQ=255)                                            ggtttGCAGGGGGTGCCCTCCTGCCCCGCAACAGGGGCCGCCAGAAGGCGTTGGGCGGTGGCCTGTGGG‑CGGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGtt                                                                              <  6:332674/133‑1 (MQ=255)                                            gCTTTGCTGGCGGTGGCCGGCGGCGCCGCCACCGGGGCCTGCAGACGGCCTTGGGCGGGGGCGTGTGGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGtt                                                                              <  8:22651/135‑1 (MQ=255)                                                             ctgctgCGCCGCCACAGCGGCCTGCAGAAGGCCTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGc                                                             >  1:362367/1‑135 (MQ=255)                                                                ctgcGCCGCCACCGCGGCCTGCAGACGGCCTTTGGCGGGGGCCGGTGGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCgg                                                          <  5:186633/135‑1 (MQ=255)                                                                ctgcGCCGCCACAGCGGCCTGCAGAAGGCGTTGGGCGGGGGCCTGTGGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCgg                                                          <  3:188883/135‑1 (MQ=255)                                                                ctgcCCCGCCACGGCGGCGGGCTGACGGCGTTGGGGGGGGGCGTGGTGC‑CGGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCgg                                                          <  7:350357/135‑1 (MQ=255)                                                                 tgcGCCGCCCCCGGGGCGGGCTTAAGGCGTTGGGGGGTGGCCTGTGGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCggt                                                         <  2:285548/135‑1 (MQ=255)                                                                 ggcgcCGCCACTGCGGCCTGCAGACGGCGTTGGGGGGGGGCGTGTGGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCggt                                                         <  5:293045/134‑1 (MQ=255)                                                                 ggcgcCGCCACGGGGGCGGGCATAGGGCGTGTGG‑GGGGGCCGGGTGGCCCGGCCCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCggt                                                         <  2:347155/134‑1 (MQ=255)                                                                          acaGCGGCCGGCAGAAGGCCTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGttt                                                <  6:192974/135‑1 (MQ=255)                                                                                  tggCAGACGGCGTTGGGCGGTGGCCTGTTGG‑CGGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCTGCTTGCCGGTGGGTGGTTTTACCTAtt                                        <  5:171368/133‑1 (MQ=255)                                                                                  gtGCAGATGGCGTTTGGGGGTGGCGTGTTGG‑CGGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTAtt                                        <  8:13012/134‑1 (MQ=255)                                                                                  ctgatgAAGGCCTTTGGGGGGGGCGTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTAtt                                        <  7:265754/130‑1 (MQ=255)                                                                                  cggCAGACGGCCTTGGGGGGGGGCGGGGTGG‑CGGGGGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTAtt                                        <  8:347660/133‑1 (MQ=255)                                                                                  cggCAGACGGCCTTGGGCGGGGGCCTGTGGG‑CGGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTAtt                                        <  5:311651/133‑1 (MQ=255)                                                                                  cTGCAGAAGGCCTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCACTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTAtt                                        <  4:245958/135‑1 (MQ=255)                                                                                   tGCTGATGGCGTTTGGGGGGGGCCTGGGGG‑CCGGGGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTc                                       <  8:348816/135‑1 (MQ=255)                                                                                   tGCAGAAGGCCTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCGTGCCGGTGGGTGGGTTTACCTATTc                                       >  5:299024/1‑135 (MQ=255)                                                                                      aGAAGGCCTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCg                                      >  8:12889/1‑133 (MQ=255)                                                                                       gcaGGCGTTGGGCGGGGGCGTGTTGG‑CCGGGGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCaa                                   <  3:375564/133‑1 (MQ=255)                                                                                             cTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTg                             >  8:89314/1‑135 (MQ=255)                                                                                              tttGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGGCGGTGGGTGGTTTTACCTATTCGCAAGGCCt                              >  2:233642/1‑133 (MQ=255)                                                                                                tGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCAGCAGTTGGCCAGCCCCGGCTTGCCCGTGGGTGGGTTTACCTATTCGCAAGGCCTggtg                          >  7:149181/1‑133 (MQ=255)                                                                                                  gCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTg                        >  3:290227/1‑135 (MQ=255)                                                                                                  gCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTg                        >  4:256140/1‑135 (MQ=255)                                                                                                  gCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTg                        >  8:10010/1‑135 (MQ=255)                                                                                                  gCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCCGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAgct                        >  5:389967/1‑133 (MQ=255)                                                                                                  gCGGGGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGt                         <  4:65553/134‑1 (MQ=255)                                                                                                   cGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTgg                       >  4:194907/1‑135 (MQ=255)                                                                                                      gggCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTGGGCg                    <  8:197034/134‑1 (MQ=255)                                                                                                        gCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTAtt                                        >  4:268286/1‑113 (MQ=255)                                                                                                        gCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTAtt                                        <  3:268286/113‑1 (MQ=255)                                                                                                        gCCTGTTGG‑CCGGCGCCGCACTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTAtt                                        <  3:17363/113‑1 (MQ=255)                                                                                                        gCCTGTTGG‑CCGGCGCCGCACTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTAtt                                        >  4:17363/1‑113 (MQ=255)                                                                                                         ccTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTGGGCGGTc                 >  4:204750/1‑135 (MQ=255)                                                                                                               gg‑cgggcgCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTGGGCGGTCGAGGcc           <  5:334395/135‑1 (MQ=255)                                                                                                                 cccGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTGGGCGGTCGAGGCCg          <  3:142512/134‑1 (MQ=255)                                                                                                                       gccgcGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTGGGCGGTCGAGGCCGGCTggg    <  7:10010/135‑1 (MQ=255)                                                                                                                       cccgcGCTGGGACTGGCCGGTTGATGACCAGCGACCGGGCGCTGCTGCGCCGGCTGCAGTTGGCCAGCCCCGGCTTGCCGGGGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTGGGCGGTCGAGGCCGGCTggg    <  5:250788/134‑1 (MQ=255)                                                                                                                        ccgcGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTGGGCGGTCGAGGCCGGCTGGGt   >  6:149411/1‑135 (MQ=255)                                                                                                                         cgcgCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTGGGCGGTCGAGGCCGGCTGGGtt  >  5:362075/1‑135 (MQ=255)                                                                                                                            |                                                                                                                                    AGCGCGGGGTTTGTCGTGGTCAGCGGCTTGCTGCTGGCGGCGGGCTTTGCAGTCGGTTGCCTGCTGCGCCGCCACAGCGGCCTGCAGAAGGCCTTTGGCGGTGGCCTGTTGG‑CCGGCGCCGCGCTGGTACTGGCCGGTTGATGAACAGCGACCTGGCGCTGCTGCGCCTGCTGCAGTTGGCCAGCCCCGGCTTGCCGGTGGGTGGTTTTACCTATTCGCAAGGCCTGGAGTGGGCGGTCGAGGCCGGCTGGGTT  >  KT2440/3244078‑3244331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑