| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,440,343 | A→C | 100% | S18R (AGC→CGC) | CJ019_03266 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,440,343 | 0 | A | C | 88.5% | 16.6 / ‑5.2 | 21 | S18R (AGC→CGC) | CJ019_03266 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (14/4); total (16/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA > KT2440/3440211‑3440473 | gATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGccgcccgc > 1:198152/1‑135 (MQ=255) aaTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCTGGCGGCGCCCgg > 3:191828/1‑134 (MQ=255) aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCAGCGCTGCCGGCGCCCggcc > 7:232326/1‑133 (MQ=255) cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTGGCGGCGCCCGGGCCGCCGGGGGGTGGCAGGCCACCGCCta > 8:44252/1‑134 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCAGGCGGCGCCCGGGCCGCCGGGGGGGGGCGGGCCCCCGccga > 1:116739/1‑131 (MQ=255) tctACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCACCGCCGATAATGCGCa > 4:204164/1‑135 (MQ=255) aaGCGAGCGCTCGAGGTGATGAAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCCGGCCCCCGCCTATAAGGCGCcgcgcg > 7:195222/1‑135 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGGGGCCGGCCAACGCCAAGAACGCGCCGCGCGCGGCTCAg > 1:232506/1‑135 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGGGGCAGGCCCCCGCCGATAAGGCGCAGCGCGCTGATCAg > 7:136842/1‑135 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCGCGTGCGGGCCGCGGGCAGCCTGGGTGTGGGATTCCCCCGCCCAGAAAGCCCAGCTCGCGGAGCaccc > 1:49318/1‑132 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGGGTGGCCGGCCCCCGCCGATAAGGCGCCGCGCGCGGGTCAGGc > 1:12869/1‑135 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCCGGGTGTGGGAGGCCCACCCCGATAACGCGCCGCGCGCGGCTCAGGc > 5:62334/1‑135 (MQ=255) gTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGGGCCCGGGCGGCCTGGGTGTGGGAGGCCAACGCCGATAAGGCGCAGCTCGCTGCTCAGGGCgcgg > 7:128253/1‑132 (MQ=255) cAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCGGGGGGGGGGCGGCCCACGCCGATAAGGCGCCGCGCGCTGCTCAGGCCGAGGACTTCg > 4:53922/1‑135 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCCGGCCCCCGCCGCTAAGGCGCAGCGCGCTGCTCAGGCCGCGGACGTCGGGcagcag > 1:25547/1‑135 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTGGCGGCGCCCGGGCCGCCGGGGGGTGGCTGGCCACCCCCGAGAAGGCGCAGCGCGCTGATCAGTCCGAGGACTTCGGCAAGCAg > 3:265196/1‑135 (MQ=255) ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCCCAGGCGGCCCCCGCGCCGCCAGGGTGTTGCACGCCAACCCCAATAAAGCCCAGCTCCCGAATAAGGCCGCAGGCATCGGCAAACAGTTTGc > 4:235557/1‑135 (MQ=255) cggcggggCGCGGCGGCGGGCCGCCCGCGCGGCCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 3:346168/129‑1 (MQ=255) cgcctgggCGCGGCTGCGGGCCGGCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 6:152275/129‑1 (MQ=255) cGCCGGGGCGCGGCGGCCGGCCGCCCGGGCGGCCCCCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 1:292181/135‑1 (MQ=255) cgggCCGCCTGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACTGTCTGGAGCgg < 3:193745/133‑1 (MQ=255) cgccCGCGGGGGCGCCGCCCGGGCAGCCTGGGTGGGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa < 4:38376/135‑1 (MQ=255) | GATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA > KT2440/3440211‑3440473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |