| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,617,883 | T→G | 44.2% | D186A (GAC→GCC) | CJ019_03428 ← | 2,6‑dihydropseudooxynicotine hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,617,883 | 0 | T | G | 44.2% | 8.4 / 8.4 | 26 | D186A (GAC→GCC) | CJ019_03428 | 2,6‑dihydropseudooxynicotine hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (10/4); new base G (10/2); total (20/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
AGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCTTGGTTGAATCCAGCCCGTTGACTTGCACCAGCAGCGGTGCCGGGCCACTCACGCCTTCT > KT2440/3617754‑3618011 | aGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGGCGCGCTTCGCCGGTGCCGGGCTGGGCGACa > 6:155085/1‑135 (MQ=255) gATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGGCGCGCGTCGCCGGTGCCGGGCGGGGCGGCAATCAg > 1:357618/1‑135 (MQ=255) gcCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCGTCGCCGGGGCCGGGCTGGGCGCCAATCCGcgacg > 2:352524/1‑135 (MQ=255) cccACTAGGCGGGTGCCCTCGTGTGCGCGGTCCGGGGGGCCCTTCAGGCGGGGGCGGCTCAGCGCGGCCCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCg < 4:277298/134‑1 (MQ=255) cccACTAGGCGGCGGGCCCTGTGGGCGCTGTCAGGGGGGCCCGGCAGGCGGGGGCGGGGACGCGGGCCGCCGGGGCCGGGCTGGGCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCg < 4:37628/134‑1 (MQ=255) ccACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGCCGCGCATCGCCGGGGGCGGGCTGGGGGTGAAGCAGGGCCGCCCCCCCGCGCCGGGCCCGCCCCGCAGGGAGGCCgc > 1:236008/1‑134 (MQ=255) aCTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGCAGCGCTGCGCCGGGGCCGGGCTGGGCGACCATCAGCGACGCCCCGCCACGCCTGGCCCGCCCGGCCGGCAGGccgccg > 7:299167/1‑135 (MQ=255) tGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGCCCATCAGCGCCGCCACGCCTCGCCTGGCCCGCCCGGCCGGCAGGCCGACGCGGGatttca > 7:348377/1‑135 (MQ=255) gCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGCAGCGCTTAGCCGGTGCCGGGCTGGTGGAGAAACAGCGACGACACCCCACGCCAGGCCAGCCAGGCACGCCCGCCGCCGCGGGagttgatt > 4:182333/1‑135 (MQ=255) gCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGCCAATCAGCGCCGCCACGCCTCGCCTGGCCCGCCAGGCAGGCAGGCCGCCGCGGTatttcatt > 2:252085/1‑135 (MQ=255) gCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCGGGGCGACAATCACCGACCCCACGCCACGCCAGGCCCGCCCGGCCGGCACGCCCCCGCGGGatttgatt > 6:43038/1‑135 (MQ=255) cAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGGAGCGCTTGGCCGGTGGCGGGCGGGTGGGCCATCAGCCGCGACACCCCGCCCCGGGCCAGCCCGGCCGGCCGGCCGCCGCGGGagttcatttgc > 5:280848/1‑133 (MQ=255) cAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCCGCCAGGCAGGCCCGCCGACGCGGTATTTCATTTcc > 8:125807/1‑135 (MQ=255) gtgcgcgGTCCTGGTGGGCCGCCAGGCCGTGGAGGCGAAGCGCGCCCCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCtt < 2:19664/130‑1 (MQ=255) tCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCGTCGCCGGTGCCGGGCGGGGGGCCAATCAGCGCCGACACGCCACGCCTGGCCAGCCCGGCCGGCCGGCCGCCGCGGGATTTCAGTTCCTTGGtt > 7:94817/1‑135 (MQ=255) gTCATGGCGTGCCGTCATGCCGTGGAGGCGCAGCGCTTAGCCGGTGCCGGGCTGGTCGCCAATAAGCGCCGCCCCCCCTAGCCGGGCCAGCCAGGCCGGCAGGCCGCCGCGGGCTTTCGTTTGCGTGGGTGGATc > 6:148106/1‑135 (MQ=255) ggCGTGCCGTCATGCCGTGGAGGCGACGCGCTTCGCCGGTGCCGGGCGGGGCGCCAATCACCGCCGCCCCGCCGCGCCAGGCCCGCCCGGCAGGCCGGCCGCCGCGGTATTTGATTTGGTTGGTTGTATCCAGcc > 3:326362/1‑135 (MQ=255) ggCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGCCAATCAGCGACGACACGCCTCGCCTGGCCCGCCAGGCAGGCCGGCCGACGCGGTATTTCATTTCCTTGGTTGAATCCCGcc > 4:87643/1‑135 (MQ=255) tGCCGTCATGCCGTGGAGGCGCAGCGCTTCGCCGGTGCCGGGCTGGGGGCCAATAAGCCCCGACACGCCTCGCCAGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTGATTTACATTGTTGAATCCAGcccct > 4:38866/1‑132 (MQ=255) tCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTGGACAATCAGCGACGCCACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGCCGCGGTATTTCATTTGCTTGGTTGAATCCAGCCCGGTGACtt > 7:354038/1‑135 (MQ=255) aGGCGAAGCGCTTCGCCGGGGCCCGGCCGGGCCACAATAAGCCACGCCAAGCCTCCCCAGGCCCGCCCCGCAGGCAGGCCGCCGCGGTATGTACATTGCTTGGTTGTATCCACCCCGGTGACATGCCCCagcggc > 2:373218/1‑135 (MQ=255) aGGCGAAGCGCGTCGCCGGTGCCGGGCTGGTCGACAATCAGCGCCGCCACGCCTCCCCTGGCCCGCCCGGCCGGCAGGCCGACGCGGTATTTCATTTCCTTGGTTGAATCCCGCCCGTTCACTTGCCCCagcagc > 8:78417/1‑135 (MQ=255) cccGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCTTGGTTGAATCCAGCCCGTTGACTTGCACCAGCAGCGGTGCCGGGcc < 1:252085/133‑1 (MQ=255) cGGTGCCGGGGTGGGCCACAATCCGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCTTGGTTGAATCCAGCCCGTTGACTTGCACCAGCAGCGGTGCCGGGCCACTCa < 5:43038/135‑1 (MQ=255) ggCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCTTGGTTGAATCCAGCCCGTTGACTTGCACCAGCAGCGGTGCCGGGCCACTCACGCCTTCt > 5:107654/1‑135 (MQ=255) ggCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCTTGGTTGAATCCAGCCCGTTGACTTGCACCAGCAGCGGTGCCGGGCCACTCACGCCTTCt < 4:337825/135‑1 (MQ=255) | AGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCTTGGTTGAATCCAGCCCGTTGACTTGCACCAGCAGCGGTGCCGGGCCACTCACGCCTTCT > KT2440/3617754‑3618011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |