Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,648,351 G→A 5.8% Q367* (CAG→TAG)  gsiA_1 ← Glutathione import ATP‑binding protein GsiA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,648,3510GA 5.8% 227.5 / 3.1 69Q367* (CAG→TAG) gsiA_1Glutathione import ATP‑binding protein GsiA
Reads supporting (aligned to +/- strand):  ref base G (32/33);  new base A (2/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.47e-01

CACCCGTGTGATCAGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTTCTCCAGGCCCAGGGCGATGCGCAGGGCGGTGCTCTT  >  KT2440/3648220‑3648483
                                                                                                                                   |                                                                                                                                    
cACCCGTGTGATCAGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGACACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCAc                                                                                                                                   <  1:303134/135‑1 (MQ=255)
 aCCCGTGTGATCAGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCa                                                                                                                                    >  3:72214/1‑133 (MQ=255)
 aCCCGTGTGATCAGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCa                                                                                                                                    >  2:264201/1‑133 (MQ=255)
 aCCCGTGTGATCAGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCa                                                                                                                                    >  7:204046/1‑133 (MQ=255)
 aCCCGTGTGATCAGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCAcc                                                                                                                                  >  8:148246/1‑135 (MQ=255)
 aCCCGTGTGATCAGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCAc                                                                                                                                   <  8:10520/134‑1 (MQ=255)
 aCCCGTGTGATCAGCTCGCGGGCCTTGCGCTCTAGTGCCGCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCAc                                                                                                                                   <  6:267115/134‑1 (MQ=255)
     gggtgAGCAGCTCGCGGGCCTTGCGCGCTAGTGCCTCGCCCCGGCGCAGGCCGAACGAGACCAGCGGCGCGACGACGCTGTCGAACACGGTGAAGCGCTGGTCGAGCGCGTCGAACGGGTTTTGCTGCACCAGCt                                                                                                                              <  7:194027/133‑1 (MQ=255)
      tgtgATCAGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGACGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCt                                                                                                                              >  2:88040/1‑134 (MQ=255)
      tgtgATCAGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCt                                                                                                                              >  5:339616/1‑134 (MQ=255)
         gATCAGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGAt                                                                                                                          >  5:140739/1‑135 (MQ=255)
             aGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGc                                                                                                                      >  2:103293/1‑135 (MQ=255)
                  gcgGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGgcg                                                                                                                 >  5:73039/1‑135 (MQ=255)
                  gcgGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGgc                                                                                                                  >  6:372224/1‑134 (MQ=255)
                   gcggCCTTGCGCTCTAGGGCCTCACCCCGGAGAAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGgc                                                                                                                  <  4:144907/131‑1 (MQ=255)
                      gCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAg                                                                                                             >  5:282113/1‑135 (MQ=255)
                        cTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAggg                                                                                                           <  4:72214/135‑1 (MQ=255)
                            cgcTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCg                                                                                                        <  4:364079/134‑1 (MQ=255)
                            cgcTCTAGGGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGGCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCg                                                                                                        <  6:147137/134‑1 (MQ=255)
                                    tGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCCAGGGGCGGAACTGGCt                                                                                               >  4:279417/1‑135 (MQ=255)
                                    tGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTgg                                                                                                 <  1:7587/133‑1 (MQ=255)
                                    tGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTg                                                                                                  <  8:204046/132‑1 (MQ=255)
                                    tGCCGCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTgg                                                                                                 <  1:75365/133‑1 (MQ=255)
                                       cgccccccGTAGCCGGCCTAAACACACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCa                                                                                            <  6:339616/131‑1 (MQ=255)
                                         cGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAgg                                                                                          >  1:212805/1‑135 (MQ=255)
                                            cccGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGgtg                                                                                        >  8:86294/1‑134 (MQ=255)
                                            cccGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGgtg                                                                                        >  8:87543/1‑134 (MQ=255)
                                              cGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGCAGCGCGGGTCGAGCGCGGCGAGCGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGgtgtca                                                                                     >  6:374052/1‑133 (MQ=255)
                                              cGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGACCTGGCTCCAGGTGTaa                                                                                     >  8:382948/1‑135 (MQ=255)
                                              cGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGgtggac                                                                                     >  2:306635/1‑132 (MQ=255)
                                              cGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTaa                                                                                     >  2:353805/1‑135 (MQ=255)
                                                         gAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTACCCGGTGACGt                                                                           >  3:118214/1‑134 (MQ=255)
                                                                  cAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGc                                                                 <  6:213214/135‑1 (MQ=255)
                                                                   aGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCg                                                                >  6:100461/1‑135 (MQ=255)
                                                                   aGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCg                                                                >  5:297423/1‑135 (MQ=255)
                                                                     cGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGaa                                                              >  5:243012/1‑135 (MQ=255)
                                                                       gCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCTAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTACTCGCCTGCGAaca                                                            >  2:204063/1‑135 (MQ=255)
                                                                        cTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAacac                                                           <  7:201191/135‑1 (MQ=255)
                                                                        cTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAacac                                                           <  7:148246/135‑1 (MQ=255)
                                                                             cgacgCTGTCGAACACGGTGACGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCt                                                      <  6:227914/135‑1 (MQ=255)
                                                                             cgacgCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCCAGGGGCGGAACTGGCTCCAGGTGTACCCGGTGACGTCCTCGCCGGCGCACCCCCCCt                                                      >  5:216983/1‑135 (MQ=255)
                                                                             cgacgCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCt                                                      <  5:306768/135‑1 (MQ=255)
                                                                                        aaCACGGTGAAGCGCTGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCCGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTg                                           <  8:312941/135‑1 (MQ=255)
                                                                                        aaCACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTg                                           <  8:190975/135‑1 (MQ=255)
                                                                                        aaCACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTg                                           <  6:297423/135‑1 (MQ=255)
                                                                                             ggTGAAGCGCGGGTCGAGCGCGNCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCtt                                      <  3:344523/135‑1 (MQ=255)
                                                                                             ggTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCtt                                      <  5:336275/135‑1 (MQ=255)
                                                                                             ggTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCtt                                      <  3:338846/135‑1 (MQ=255)
                                                                                             ggTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCtt                                      <  8:26001/135‑1 (MQ=255)
                                                                                              gTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTtc                                     <  8:115990/135‑1 (MQ=255)
                                                                                              gTGAAGCGCGGGTCGAGCGCGGCGAACGGGGTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTtc                                     <  3:39808/135‑1 (MQ=255)
                                                                                                gAATCGCGGGTCGAGCGCGGCGAAGGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCTTAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTtctc                                   <  7:42939/135‑1 (MQ=255)
                                                                                                gAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGgc                                                                                                                  >  6:312616/1‑56 (MQ=255)
                                                                                                gAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGgc                                                                                                                  <  5:312616/56‑1 (MQ=255)
                                                                                                gAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGATCCATGTGTAACCGGAGACGTACTCGCCGGCGAACACCACCTGCCCTTGACTGGTCTtctc                                   >  2:67470/1‑135 (MQ=255)
                                                                                                  aGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTTCTCCa                                 >  7:282743/1‑135 (MQ=255)
                                                                                                  aGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTACACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTg                                           >  6:99766/1‑125 (MQ=255)
                                                                                                  aGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTACACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTg                                           <  5:105332/125‑1 (MQ=255)
                                                                                                  aGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTACACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTg                                           >  6:105332/1‑125 (MQ=255)
                                                                                                  aGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTACACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTg                                           <  5:99766/125‑1 (MQ=255)
                                                                                                     gcgGGTCGCGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTTCTCCAGGc                              <  1:353805/135‑1 (MQ=255)
                                                                                                     gcgGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAg                                                                                                             >  5:147209/1‑56 (MQ=255)
                                                                                                     gcgGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAg                                                                                                             <  6:147209/56‑1 (MQ=255)
                                                                                                          tCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTTCTCCAGGCCCAgg                         >  2:119996/1‑135 (MQ=255)
                                                                                                          gcGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTTCTCCAGGCCCAgg                         <  2:95102/134‑1 (MQ=255)
                                                                                                            gAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCtt                                                <  4:201917/110‑1 (MQ=255)
                                                                                                            gAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCtt                                                >  3:201917/1‑110 (MQ=255)
                                                                                                            gAGCGCGGCGAACGGGGTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTTCTCCAGGCCCAGGGc                       <  1:236150/135‑1 (MQ=255)
                                                                                                                gcggcgAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTTCTCCAGGCCCAGGGCGATg                   >  1:136096/1‑135 (MQ=255)
                                                                                                                                 ctgcACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTTCTCCAGGCCCCGGGCGATGCGCAGGGCGGTGCTCtt  >  4:355018/1‑135 (MQ=255)
                                                                                                                                  tgcACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTTCTCCAGGCCCAGGGCGATGCGCAGGGCGGTGCTCtt  <  3:61053/134‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                    
CACCCGTGTGATCAGCTCGCGGGCCTTGCGCTCTAGTGCCTCGCCCCGGAGCAGGCCGAAAGAGACCAGCGGCTCGACGACGCTGTCGAACACGGTGAAGCGCGGGTCGAGCGCGGCGAACGGGTTTTGCTGCACCAGCTGGATACGCCGGCGCAAGGGGCGGAACTGGCTCCAGGTGTAACCGGTGACGTCCTCGCCGGCGAACACCACCTGCCCTTGACTGGGCTTCTCCAGGCCCAGGGCGATGCGCAGGGCGGTGCTCTT  >  KT2440/3648220‑3648483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑