| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,661,125 | C→A | 6.6% | G207C (GGC→TGC) | CJ019_03469 ← | putative protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,661,125 | 0 | C | A | 6.6% | 161.6 / 4.2 | 61 | G207C (GGC→TGC) | CJ019_03469 | putative protein |
| Reads supporting (aligned to +/- strand): ref base C (34/23); new base A (2/2); total (36/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.07e-02 | |||||||||||
CTCTGCGGCGATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTCCAGATACGCCAGGACTTCATGGATGGAAT > KT2440/3660991‑3661259 | ctctGCGGCGATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGcc > 7:165355/1‑135 (MQ=255)ctctGCGGCGATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGcc > 7:75602/1‑135 (MQ=255) tGCGGCGATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGTGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACt > 4:50138/1‑135 (MQ=255) tGCGGCGATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACt > 3:97586/1‑135 (MQ=255) tGCGGCGATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACt > 6:369328/1‑135 (MQ=255) tGCGGCGATGATTACACGGGCAGTCTGCGTGGCGTTGAAAACAGCTTCAGCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCGGTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGCCGCCACt > 2:369163/1‑135 (MQ=255) cggcgATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACtgt > 7:327514/1‑135 (MQ=255) cggcgATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACtgt > 4:373760/1‑135 (MQ=255) cggcgATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACtgt > 2:235960/1‑135 (MQ=255) cggcgATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACtgt > 3:170143/1‑135 (MQ=255) cggcgATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACtgt > 3:165861/1‑135 (MQ=255) cggcgATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACtgt > 5:22454/1‑135 (MQ=255) cggcgATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTCGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACtgt > 3:54063/1‑135 (MQ=255) cgATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCt > 2:37534/1‑135 (MQ=255) cgATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCt > 8:373529/1‑135 (MQ=255) atgatTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCg < 5:356777/135‑1 (MQ=255) gcatTCCACGGGCATTCTGCGGGGCGTTGAAAAAAGCTTCATCATGGCGGACAAGCCCTGACAATTGCGCGGCGAGCATGGCATTCCGTTGCTGGATCCCCGGTTGCGCGCATGGCATGACGCCACTGTGCTCGa < 6:225783/133‑1 (MQ=255) atTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGAc > 7:60138/1‑134 (MQ=255) acacGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAg > 8:154983/1‑135 (MQ=255) ttCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTAc > 6:257717/1‑135 (MQ=255) tCTGCGTGGCGTTGAAAACAGCTTCATCATTGAGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGGTGCTGTATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACa < 8:305848/135‑1 (MQ=255) tCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACa > 4:320815/1‑135 (MQ=255) gTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGcgcg < 8:165355/135‑1 (MQ=255) ggCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCggg > 8:120071/1‑135 (MQ=255) tGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGgtcgt > 1:21580/1‑135 (MQ=255) aaaaGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCt < 5:369328/134‑1 (MQ=255) catTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCa < 2:287432/135‑1 (MQ=255) catTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGGTGCGGGATCCCGGGTGGGTCGCTTGGCATCACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCa < 2:198995/135‑1 (MQ=255) ggACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAAc > 3:256310/1‑135 (MQ=255) gACAAGCCCAGACAATTTCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAAcc > 2:253177/1‑135 (MQ=255) aCAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCg < 3:374136/135‑1 (MQ=255) ccTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAgcgg < 2:111679/135‑1 (MQ=255) cAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGAc < 1:385614/134‑1 (MQ=255) aaTTGCTCGGCGCGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGCTCGTCCTGCCAAGCCGTCAGGCAACCGTAGCGGCGACgg > 4:276868/1‑135 (MQ=255) aTTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGcc > 3:267007/1‑61 (MQ=255) aTTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGcc < 4:267007/61‑1 (MQ=255) tGCTCGGCGAGCATGGCATTCAGTTGCTGGCTCACCGGTTGCTCGCATGGCAGGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCt < 3:256205/135‑1 (MQ=255) tGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCt < 4:377118/135‑1 (MQ=255) tGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCt < 3:235831/135‑1 (MQ=255) gCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGGTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCt < 7:154983/134‑1 (MQ=255) tCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCa > 7:322922/1‑135 (MQ=255) gAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCAACTGTGCTCGa < 7:224737/62‑1 (MQ=255) gAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCAACTGTGCTCGa < 7:230636/62‑1 (MQ=255) gAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCAACTGTGCTCGa > 8:230636/1‑62 (MQ=255) gAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCAACTGTGCTCGa > 8:224737/1‑62 (MQ=255) gCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATggg > 7:218372/1‑132 (MQ=255) gtcgCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCg < 4:54063/131‑1 (MQ=255) gTTGCTGGCTCCCCGTTTGCACGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTAGAGCGCGGGGGCGTCCTGCAAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCg < 5:369489/134‑1 (MQ=255) ttGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGAc > 7:102139/1‑135 (MQ=255) gCTGGAGCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGAccc < 2:387189/135‑1 (MQ=255) cTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAg < 4:321160/49‑1 (MQ=255) cTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAg > 3:321160/1‑49 (MQ=255) cTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGAccc < 1:253177/134‑1 (MQ=255) gATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGt > 7:138917/1‑134 (MQ=255) gATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGt > 8:57796/1‑134 (MQ=255) gATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTc > 1:309287/1‑135 (MQ=255) gATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCACGAAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTc > 2:156873/1‑135 (MQ=255) agccccGGTTGCTCGCAGGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGGCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTcc < 6:1340/131‑1 (MQ=255) ccGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCACCCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTCCAGAt > 7:206939/1‑135 (MQ=255) ggTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCCGGCAACCGTAGCGGCGACGGGCGTCATGGGCCAGATGCCGGTCGACCCGGTCCAgctcc > 2:97236/1‑131 (MQ=255) gcatggcatgACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTCCAGATACGCCAGGAc > 5:181861/1‑135 (MQ=255) gcatggcatgACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTCCAGATACGCCAGGAc > 2:279075/1‑135 (MQ=255) atgtcatgACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTCCAGATACGCCAGGACtt < 5:357726/135‑1 (MQ=255) catgACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTCCAGATACGCCAGGACTTCat < 4:256310/134‑1 (MQ=255) gACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTCCAGATACGCCAGGACTTCatggat < 3:50138/135‑1 (MQ=255) cACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTCCAGATACGCCAGGACTTCATGGATGGAAt < 8:212327/135‑1 (MQ=255) | CTCTGCGGCGATGATTACACGGGCATTCTGCGTGGCGTTGAAAAAAGCTTCATCATTGCGGACAAGCCCTGACAATTGCTCGGCGAGCATGGCATTCAGTTGCTGGATCACCGGTTGCTCGCATGGCATGACGCCACTGTGCTCGACAAAGTGCCCGTACAGCGCGGGGTCGTCCTGCCAAGGCGTCAGGCAACCGTAGCGGCGACGGGCTTCATGGGCCAGATGCGGGTCGACCCGGTCCAGATACGCCAGGACTTCATGGATGGAAT > KT2440/3660991‑3661259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |