Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,848,001 A→C 22.1% F283V (TTT→GTT)  CJ019_03645 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,848,0010AC22.1% 132.9 / 5.4 79F283V (TTT→GTT) CJ019_03645hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (31/28);  new base C (2/18);  total (33/46)
Fisher's exact test for biased strand distribution p-value = 1.23e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGTTCGAATGCATTAGCAGACTTAACGACGAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGC  >  KT2440/3847868‑3848131
                                                                                                                                      |                                                                                                                                  
aGTGTTCGAATGCATTAGCAGACTTAACGACGAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAAc                                                                                                                                   >  3:71446/1‑135 (MQ=255)
 gtgtTCGAATGCATTAGCAGACTTAACGACGAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAAcc                                                                                                                                  <  3:286137/135‑1 (MQ=255)
 gtgtTCGAATGCATTAGCAGACTTAACGACGAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAAcc                                                                                                                                  <  7:343953/135‑1 (MQ=255)
 gtgtTCGAATGCATTAGCAGACTTAACGACGAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAAcc                                                                                                                                  <  2:138907/135‑1 (MQ=255)
 gtgtTCGAATGCATTAGCAGACTTAACGACGAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGGCCTGCATCTGCCCAAAcc                                                                                                                                  <  8:13451/135‑1 (MQ=255)
 gtgtTCGAATGCATTAGCAGACTTAACGACGAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGAGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAAcc                                                                                                                                  <  2:22477/135‑1 (MQ=255)
 gtgtTCGAATGCATTAGCAGACTTAACGACGAGTGGTCTCGGTCAACTAGGCGGTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAAcc                                                                                                                                  <  6:193615/135‑1 (MQ=255)
                  cAGACTTAACGACGAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCATGCATCTGCCAAAACCGCCCGGCATGGAAatca                                                                                                                 >  6:389841/1‑135 (MQ=255)
                          acgacgAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAACCCGCCCGGCATGGAAATCATCGAACGa                                                                                                         >  8:340767/1‑135 (MQ=255)
                          acgacgAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAatcatc                                                                                                               >  8:349890/1‑129 (MQ=255)
                          acgacgAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAatcatc                                                                                                               <  7:349890/129‑1 (MQ=255)
                          acgacgAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACgc                                                                                                         >  4:109959/1‑134 (MQ=255)
                          acgacgAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGa                                                                                                         >  3:18181/1‑135 (MQ=255)
                          acgacgAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGa                                                                                                         >  2:67114/1‑135 (MQ=255)
                                aGTGGTGTCGGTCAACTCGGCGTTGGCCAGGCCCCAGCCGGGAACGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAACCCGCCCGGCATGGAAATCATCGCACGACTCGGc                                                                                                   <  7:184034/135‑1 (MQ=255)
                                aGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCCGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCACCCCTCACGTCGTGCATCTGCCCAAACCGCCCGGCATGCAAATCAGCGAACGACTCGGc                                                                                                   <  3:109959/135‑1 (MQ=255)
                                aGTGGGCGCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGAATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGc                                                                                                   <  6:112434/135‑1 (MQ=255)
                                  tGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTAGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCaa                                                                                                 >  4:197616/1‑135 (MQ=255)
                                   ggTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAAcc                                                                                                                                  >  3:59500/1‑101 (MQ=255)
                                   ggTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAAcc                                                                                                                                  <  4:59500/101‑1 (MQ=255)
                                   ggTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCaa                                                                                                 >  4:300566/1‑134 (MQ=255)
                                   ggTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAAt                                                                                                >  3:200193/1‑135 (MQ=255)
                                   ggTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAAt                                                                                                >  3:73656/1‑135 (MQ=255)
                                    gTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCCACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGAATCTGCCCAAACCCCCCCGCATGGAAAACAAACAACGACACCGCAAt                                                                                                >  2:181926/1‑134 (MQ=255)
                                          gTCAACTCGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCg                                                                                           <  1:269162/133‑1 (MQ=255)
                                             aaCTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTgg                                                                                      >  4:51683/1‑135 (MQ=255)
                                               ctcggCGGTGGCCAGGCCCCAGCCGGGAAAGGCAAACAAATTCCATCCGTGGGCGG‑CGCCCCCCCTCTCGTCCTCCAGCTGCCTAAACCGCCCGGCATGCAAATCATCGCACGACTCGGCAATCTCCGCCTGGCt                                                                                    <  2:358611/132‑1 (MQ=255)
                                               cTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCt                                                                                    >  6:273222/1‑135 (MQ=255)
                                                  ggCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGt                                                                                  >  4:236662/1‑134 (MQ=255)
                                                      ttGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTGGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTtcatc                                                                             >  1:154293/1‑135 (MQ=255)
                                                         gCCAGGCCCCAGCGGGGAAAGGCCAACCAATTGCATCAGTGGGGGG‑CGCCCCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCAc                                                                           <  1:42382/134‑1 (MQ=255)
                                                         gCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCAc                                                                           <  7:256677/134‑1 (MQ=255)
                                                         gCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACg                                                                          <  1:67114/135‑1 (MQ=255)
                                                           cAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCGCGTCCTGCATCTGCCCAACCCGCCCGGCATGGAAATCATCGAACGCCTCGGCAATCTCCGCCTGGCTGTTCCTCCCGaa                                                                        >  8:339372/1‑135 (MQ=255)
                                                            aGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAAc                                                                       >  7:75721/1‑135 (MQ=255)
                                                            aGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAAc                                                                       >  2:29037/1‑135 (MQ=255)
                                                                      cGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAg                                                              >  4:84103/1‑134 (MQ=255)
                                                                           taGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCGGCATCTGCCCAAACCGCCCGGCATGGAAATCATAGAACGACTCGGCAATCTCCGCCTGCCTGTTCATCACGAACGGGCCGTAGCCAAc                                                         >  4:385497/2‑134 (MQ=255)
                                                                             gcccaaccaaTGGCAGCCGTGTGCGG‑CGCCGCCCCCCGCGTCCTGCAGCTGCCCAAACCGCCCGGCATGCAAAGCATCGCACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGAt                                                      <  5:15233/133‑1 (MQ=255)
                                                                                 aaccaaTTGCATCAGGGTGCGG‑CGCCTCCCCTCTTGTCCTGCAGCTGCCCAACCCGCCCGGCATGGAAATCACCGCACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGc                                                  <  3:197616/135‑1 (MQ=255)
                                                                                 aaccaaTTGCACCCG‑GGGCGGCCGCCGCCCCTCTCGTCCTGCATCTGCCCAACCCCCCCGGCAGGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGc                                                  <  2:294039/135‑1 (MQ=255)
                                                                                      aTTGCATCCGCGTGCGG‑CGCATCCCCTCTCGTCCGCCTCCGGCCCACCCCGCCCGGCATGTAAATCATCGCACGAGTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCtcgtc                                             <  2:43308/135‑1 (MQ=255)
                                                                                      aTTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCtcgtc                                             <  4:177414/135‑1 (MQ=255)
                                                                                      aTTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGCCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCtcgtc                                             <  8:59783/135‑1 (MQ=255)
                                                                                      aTTGCATCAGTGTGCGG‑CGCCTCCCCGCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCtcgtc                                             <  6:389246/135‑1 (MQ=255)
                                                                                      aTTGCAGCAGTGTGCGG‑CGCCTCCCCTCGCGTCCTGCATCTGCCCAACCCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCCGTTCATCACGAACGGGCCGTAGCCAACGATCGGCtcgtc                                             <  4:73656/135‑1 (MQ=255)
                                                                                           aTCAGTGTGCGG‑CGCCTCCCCTCGCGTCCTGCATCTGCCCAACCCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCg                                        <  4:131143/135‑1 (MQ=255)
                                                                                           aTCAGTGGGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCg                                        <  4:311027/135‑1 (MQ=255)
                                                                                                ggtgCGC‑GGCCCCCCCTCAGGCCCTGCATCGGCCCAACCCGCCCGGCATGCAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCa                                   <  4:242208/134‑1 (MQ=255)
                                                                                                 gcgggg‑cgCCTCACCTCGCGCCCTGCATCGCCCCAACCCGCCCGCCATGCAAATAACCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCt                                     <  1:181926/128‑1 (MQ=255)
                                                                                                    cgg‑cgCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGc                               >  3:259065/1‑135 (MQ=255)
                                                                                                     gg‑cgCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGTAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCt                              <  1:212375/135‑1 (MQ=255)
                                                                                                     gg‑cgCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCt                              >  6:221415/1‑135 (MQ=255)
                                                                                                     gg‑cgCCTCCCCTCTCGGCCTGCATCTCCCCAAACCGCCCGGAATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCt                              <  7:277285/135‑1 (MQ=255)
                                                                                                        cgcCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGa                            >  1:50043/1‑135 (MQ=255)
                                                                                                        cgcCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGa                            >  5:385762/1‑135 (MQ=255)
                                                                                                        cgcCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTGCATCACGCACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGa                            >  8:116943/1‑135 (MQ=255)
                                                                                                         gcCTCCCCTCTCGTCCTGCATCTGCCCAAACCCCCCGGCAGGGAAATCAGCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGag                           <  3:133394/135‑1 (MQ=255)
                                                                                                         gcCTCCCCTCTCGTCCTGCATCTGCCAAAACCGCCCGGCATTGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGag                           <  3:156824/135‑1 (MQ=255)
                                                                                                         gcCTCCCCTCTCGGCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGag                           <  5:170975/135‑1 (MQ=255)
                                                                                                            tCCCCTCTCGTCCTGCATCTGCCCAACCCGCCCGGCATGGAAAGCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcag                        <  4:198852/135‑1 (MQ=255)
                                                                                                            tCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcag                        <  3:300566/135‑1 (MQ=255)
                                                                                                            tCCCCTCTCGTCCTGCAGCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcag                        <  3:45234/135‑1 (MQ=255)
                                                                                                            tCCCCTCTCGGCCTGCAAGTGCCCAACCCCCCCGGAATGCAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcag                        <  7:277836/135‑1 (MQ=255)
                                                                                                            gccccTCTCGACCTGCATCTGCCCAACCCGCCCGGCATGGAAACCACCGCACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcag                        <  6:329050/134‑1 (MQ=255)
                                                                                                            ccccgTCTCTTCCGGCATCGCCCCAACCCGCCCCGCATGCAAAGAATCCCACGACGCGGCAATCTCCGCCTGGCTGTTCATCACTAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcag                        <  5:17646/134‑1 (MQ=255)
                                                                                                            cccccTCTCGTCCTGAATTTGACCCACCCCCCCGGGATGGAAATCATCGCACGAGTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCTCCGCTGagcag                        <  2:153819/134‑1 (MQ=255)
                                                                                                            cccccTCTCGCCCTGCCTCTGCCAAACCCCCCCGGCTGGTAAAACAGCCCACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcag                        <  1:192403/134‑1 (MQ=255)
                                                                                                            cccccTCGCGTCCGCCATCTCCCCAACCCGCCCGGCTCGGAACTAATCCACCGACTCGGCAATCTCCGCCTGGCTGGTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcag                        <  1:126027/134‑1 (MQ=255)
                                                                                                              cccTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcagca                      >  8:132023/1‑135 (MQ=255)
                                                                                                              cccTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcagca                      >  3:212791/1‑135 (MQ=255)
                                                                                                              cccGCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcagca                      >  8:238202/1‑135 (MQ=255)
                                                                                                                ctctCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATAGAACGACTCGGCAATCTCCTCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGagcagcagc                    >  4:178613/1‑135 (MQ=255)
                                                                                                                      tccgccATCCGCCCAACCCGCCCGGCCTCGAACTCCCCCCACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACtt              <  3:309/130‑1 (MQ=255)
                                                                                                                      tCCTGCATCTGCCCAACCCGCCCGGAATGTAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACtt              <  7:105461/135‑1 (MQ=255)
                                                                                                                             tCTGCCCAACCCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGt       <  5:221415/135‑1 (MQ=255)
                                                                                                                              cTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTc      >  2:114015/1‑135 (MQ=255)
                                                                                                                                  ccAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTc      <  2:208815/131‑1 (MQ=255)
                                                                                                                                  ccAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAgc  >  7:205669/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                  
AGTGTTCGAATGCATTAGCAGACTTAACGACGAGTGGTCTCGGTCAACTAGGCGTTGGCCAGGCCCCAGCCGGGAAAGGCCAACCAATTGCATCAGTGTGCGG‑CGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGC  >  KT2440/3847868‑3848131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑