| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,908,501 | T→G | 41.7% | intergenic (‑70/‑176) | rstA_2 ← / → acrE_2 | Transcriptional regulatory protein RstA/Multidrug export protein AcrE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,908,501 | 0 | T | G | 41.7% | 20.1 / 2.4 | 37 | intergenic (‑70/‑176) | rstA_2/acrE_2 | Transcriptional regulatory protein RstA/Multidrug export protein AcrE |
| Reads supporting (aligned to +/- strand): ref base T (13/6); new base G (2/10); total (15/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.15e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGGTAAACCCGCTGCTGCAGGTCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTCACACTCGCCCTACAGGTATTAACAGCCGCCCA > KT2440/3908370‑3908629 | aGGTAGCTGGCGATCAGCTCGGACAGGGCGCGGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGGTAAACCCGCTGCTGCAGGTccc < 2:380475/135‑1 (MQ=255) tGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGGTAAACCCGCTGCTGCCGGTCCCCGT‑AGGa > 8:72145/1‑135 (MQ=255) cGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGGTAAACCCGCTGCTGCAGGTCCCCGT‑AGGAGc < 3:248642/134‑1 (MQ=255) gATCTGCTCGGACTGGGCGCTGTCGTCTTCGACCAGGAAAATGTTGGGCATAGGTGCTGGGGCGTCTGGGGTGAAGTAAGCCTGTGGCGAGCGCTTGGCGGGTAAACCCGGTGCTGCAGGTCCCCGT‑AGGAGCgg < 8:56440/135‑1 (MQ=255) aGCTCGGACAGGGCGCTGTCGTCTTAGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCCGGTAAACCCCCGGGTGCAAGGGCCCGG‑ATCAGCGGGGTc > 7:59193/1‑135 (MQ=255) aCAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGGTTAACCCGCTGCTGCAGGTCCCCGG‑AGGAGCGGGTTCGCCcgc > 3:121917/1‑134 (MQ=255) cTGTCGTCGTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCCGGTAAACCCGCGGCAGCAGGTCCCCGG‑AGGCGCCGGTTCCCCCCCGAAGCGGcc > 4:70851/1‑135 (MQ=255) tCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGGTAAACCCGCTGCTGCAGGTCCCCGG‑AGGAGCGGGTGCACCCGCGAAGAGGCCAGCACACGc > 8:322929/1‑135 (MQ=255) gACCAGCAAAAGGTGCGGCATCGGGGCTGGGGCGGCGGGGTTCAAGTACGCCGGGGGCTAGCGCGGCGCGGTAAACCCCGCGGCGGCCGGCCCCCGT‑CGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGccc < 8:44649/135‑1 (MQ=12) ccccAGTAAAATGTGCGGCTTCGGGGGTGGGGCGTGGGGGTTCAAGTAAGCCGGGGGCGAGCGCTGCGCGGGAAACCCCGCGGCTGCAGGCCCCCGT‑AGGCGCGGGTTCACCCGCGAAGAGGCCAGCACAGGccc < 2:97469/133‑1 (MQ=19) caCCAGTAAAATGTTCGGCATAGGTGCTGGGGCGGCTGGGGTGAAGTACGCCTGGGGCGAGCGCTGCGGGGTAAAACCCGCTGCTGCCGGGCCCCGT‑CGGCGCGGGTTCACCCGCGACGAGGCCAGCACAgg < 2:220829/131‑1 (MQ=255) ccAGGAAAATGTGCGGCATCGGGGCTGGGGCGGCTGGGTTGAAGTAAGCCTGTGGGGAGCGCTGCGCGGGAAACCCCGCTGCTGCAGGGCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCAt < 8:370404/135‑1 (MQ=37) ccAGCAAAAGGTTCGGCAGTGGGGCTGGGGCGTCTGGGTTGACGTACGCCGGTGGCGAGCGCGGCGCGGTTAACCCCGCGGCTGCAGGCCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCAt < 8:192056/135‑1 (MQ=21) aTAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGGTAAACCCGCTGCTGCCGGTCCCCGT‑AGGAGCGGGTTCCCCCGCGAAGAGGCCAGCACAGGCCCCTGCAGAATATAGAACCCa > 5:143375/1‑135 (MQ=255) gCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGGTAAACCCGCTGCTGCAGGTCCCCGT‑AGGAGCGGGGTCACCCGCGCAGACGCCAGCACAGGCCCATGCCGAAAATATAACCCCACCCCTCGACAg > 3:70478/1‑135 (MQ=37) gCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCCGGTAAACCCGCTGCTGCACGTACCCGG‑AGGAGCGGGGTCACCCGCCAAGAGGCCACCACAGGCCCCAGCAGCATATAGCACCCAACCAcacccaa > 6:270892/1‑128 (MQ=255) gggTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGGTAAACCCGCTGCTGCAGGTCCCCGT‑AGGAGCGGGTTCCCCCGCGCAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCCCGCGACAGGATCa > 1:288223/1‑134 (MQ=255) gggTTGAAGTAAGCCTGTGGCGAGCGCTAAGCGGGTAAACCCGCTGCTGCAGGTCCCCGT‑ATGAGCGGGTTCACCCCCGAAGAGGCCAGCACAGGCCCATCCAGAATATAGATCCCAACCACTCGACACGAACa > 1:13217/1‑134 (MQ=38) gTTTAATTACGCCTGTGGCTAGCGCTTCGCGGGTAAACCCGCTGCTGCCGGTCCCCGT‑AGGAGCGGTTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCAtt < 3:184095/134‑1 (MQ=38) gCCTGTGGCGAGCGCTTCGCGGGTTAACCCGCTGCTGCAGGGCCCCGT‑TGGCGCGGGTTCACCCGCGAAGAGGCCCGCACCGGCCCATGGAGAATATAGAACCCCACCCCTCGCCAGGATCATTTTTgacccgtt > 4:11408/1‑130 (MQ=25) tgGCGAGCGCTTCGCGGGTAAACCCGCTGCTCCAGGGCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTcac < 7:313932/135‑1 (MQ=255) tgGCGAGCGCTGCGCGGTTACCCGCGCTGC‑GCCAGCCCCCGTGAGGCGCTGGTACCCCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTcac < 4:3471/135‑1 (MQ=255) gggCTAGCGCTGCGCGGTAAACCCCGCTGCGGCTGGCCCCGGT‑CGGCGCGGTTACACCGGCTAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTcac < 4:325363/134‑1 (MQ=255) gggCGAGGGCTGCGCGGGAAAACCCGGTGCTCCCGGGCCCCGT‑AGGAGCGGGTAAACCCGCTAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTcac < 2:248302/134‑1 (MQ=255) gggCGAGCGCTGCGCGGGTAAACCCGCTGCTGCAGGGCCCCGA‑CGGCGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTcac < 4:177740/134‑1 (MQ=39) ggCGAGCGCTTCGGGGGTAAACCCGCTGCTGCAGGGCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTcac < 4:121917/134‑1 (MQ=255) ggCGAGCGCTTCGCGGGTAACCCCGCTGCTGCAGGCCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTcac < 7:388672/134‑1 (MQ=255) gcTTCGCGGGTAAACCCGCTGCTGCAGGTCCCCGG‑AGGCGCGGGTTCACCCGCGAAGAGGGCCGCACAGGCCCAAGCAGAATATAGCACCCAACCCCTAGACAGGAACATTATTAACACTTTTTGACACTCGcc > 8:164923/1‑134 (MQ=37) gcTGCGCGGGTAAACCCGCTGCTGCAGGGCCCCGT‑AGGAGGGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTCACACTCGcc < 7:293766/134‑1 (MQ=255) ccGCTGCTGCGGGGCCCCGT‑ACGCGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTCACACTCGCCCTACAGGTATTAACAg < 7:120835/135‑1 (MQ=255) ggtgctgcAGGGCCCCGT‑AGTTGGGGTTTAACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTCACACTCGCCCTACAGGTATTAACAgcc < 3:196126/133‑1 (MQ=255) cggcAGGGCCCCGT‑CGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTCACACTCGACCTACAGGTATTAACAGCCGccc < 2:26625/133‑1 (MQ=255) tgcAGGTCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTcac > 2:329440/1‑105 (MQ=255) tgcAGGTCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTcac < 1:329440/105‑1 (MQ=255) tgcAGGTCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTCACACTCGCCCTACAGGTATTAACAGCCGCCCa > 7:79709/1‑135 (MQ=255) gTCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCAt > 6:167440/1‑48 (MQ=255) gTCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCAt < 5:167440/48‑1 (MQ=255) | AGGTAGCTGGCGATCAGCTCGGACAGGGCGCTGTCGTCTTCGACCAGGAAAATGTTCGGCATAGGTGCTTGGGCGTCTGGGTTGAAGTAAGCCTGTGGCGAGCGCTTCGCGGGTAAACCCGCTGCTGCAGGTCCCCGT‑AGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCCCATGCAGAATATAGAACCCAACCACTCGACAGGATCATTCTTCACACTTTTTCACACTCGCCCTACAGGTATTAACAGCCGCCCA > KT2440/3908370‑3908629 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |