| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,909,205 | A→C | 20.9% | T177P (ACC→CCC) | acrE_2 → | Multidrug export protein AcrE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,909,205 | 0 | A | C | 20.9% | 142.2 / 6.1 | 81 | T177P (ACC→CCC) | acrE_2 | Multidrug export protein AcrE |
| Reads supporting (aligned to +/- strand): ref base A (41/20); new base C (3/16); total (45/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTCGACGACAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATGTCACCCAGTCTACCC > KT2440/3909071‑3909337 | tGGTCGCCGACAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑a > 4:309500/1‑135 (MQ=255)tGGTCGACGACAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑a > 1:135026/1‑135 (MQ=255)tGGTCGACGACAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑a > 4:28601/1‑135 (MQ=255)tGGTCGACGACAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑a > 6:113544/1‑135 (MQ=255)tGGTCGACGACAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑a > 3:261265/1‑135 (MQ=255) tCGACGACAAAGCCGTCAGCCGCCAGTAATACGACAACGCCTAGGCAAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGATCCTCGGCTATGCCACCGTT‑Acag < 4:73792/135‑3 (MQ=255) cgacgaCAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGCCCCTCGGCTATGCCACCGTG‑CCcgc > 5:291856/1‑135 (MQ=255) gacgacAAAGCCGTCAGCCGCCAGGAATACGACACCGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGCACCTCGGCGATGCCACCGAG‑ACcgcg > 1:378494/1‑135 (MQ=255) gacgacAAAGCCGTCAGCCGCCAGGAATACGACAACGCCCAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCCGGGCGGCGCTGGAGCGCGCCCGGCTGCACCTCGGCTATGCCACCGTG‑ACcgcg > 7:249954/1‑135 (MQ=255) gacgacAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGCGCGCGCCCTGCTGAACCTCGGCTATGCCACCGTG‑CCcgcg > 5:284961/1‑135 (MQ=255) gacgacAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGCACCTCGGCTATGCCACCGTG‑CCcgcg > 8:267658/1‑135 (MQ=255) gacgacAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACcgcg < 3:332230/135‑1 (MQ=255) acgacAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACcgcg < 5:202628/134‑1 (MQ=255) acAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGa < 5:64895/135‑1 (MQ=255) cAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGAt < 1:208489/135‑1 (MQ=255) cAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGAt < 4:178574/135‑1 (MQ=255) aaGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGAtct < 1:374213/135‑1 (MQ=255) aaGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGAtct < 4:248662/135‑1 (MQ=255) gCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTcc < 8:351377/135‑1 (MQ=255) gCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTcc < 8:47725/135‑1 (MQ=255) cGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTCGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGGT‑GCCGCGCCGATCTcccg > 5:60011/1‑133 (MQ=255) ccgccAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCTATCTCCGGCCGTAt < 3:348775/135‑1 (MQ=255) cgccAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCCGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATc > 7:297047/1‑135 (MQ=255) gccAGTAATACGACACCGCCAAGGCCAGTTTCCTGCCGGCCTATGCCGATGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTTAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTAGCg < 8:297047/135‑1 (MQ=255) cGACAACGCCACGGCCCGTTTCCTGCCGGCCGATGCCGATGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGACCTCCGGCCGTATCGGCCGCGCCCa < 4:133439/135‑1 (MQ=255) cAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGt > 6:89129/1‑135 (MQ=255) cAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGCTCTCCGGCCGTATCGGCCGCGCCCAGGTCAcc > 5:104893/1‑133 (MQ=255) cAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGCTCTCCGGCCGTATCGGCCGCGCCCAGGTCAcc > 3:379679/1‑133 (MQ=255) cAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCAcc > 5:133674/1‑133 (MQ=255) cAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCAcc > 6:291218/1‑133 (MQ=255) cAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCAcc > 1:235073/1‑133 (MQ=255) cAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCAcc > 3:382791/1‑133 (MQ=255) ccAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGgcg > 2:116998/1‑135 (MQ=255) ccAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCACCGGCCGTATCGGCCGCGCCCACGGCACCGCAGgcc > 6:29215/1‑134 (MQ=255) cAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGCACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGgcgc > 4:117421/1‑135 (MQ=255) aGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCggg > 3:345015/1‑134 (MQ=255) aGGCCGATGCCGAAGTGGCCAAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCggg > 7:133985/1‑134 (MQ=255) ggCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTgggcgggc > 8:344292/1‑134 (MQ=255) gATGCCGAAGTGGTCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCCCGTCCCCCAACGCGCGCAGGTCGGGCaaaa > 2:210095/1‑134 (MQ=38) gATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGCACCTCGGCTATGCCACCGTG‑ACCGCGCCGCTCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAAc > 6:216223/1‑135 (MQ=255) gATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCaaaa > 7:168489/1‑134 (MQ=255) cGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCCCCGAAGGCGCGCTGGTCGGGCAAAACGAAac > 2:354241/1‑135 (MQ=255) ggggCCGACGCCCGGGCGGCGGTGCCGCGCGCCGGGCTAACCCGCGGCAAGCCCCCGGTC‑CCCGCGCCGAGCTCCGCCCGTATCGCCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAaccacc < 6:313047/133‑1 (MQ=255) cGACGCCCGGGCGGCGCTGGAGCGCGCCCGGCTGAACCGCGGCTATGCCACCGTG‑ACCGCGCCGATCGCCGGCCGTAGCGCCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCt < 2:235073/135‑1 (MQ=255) gAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGCAACCACCCCGCTg > 8:266173/1‑135 (MQ=255) gAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTg > 5:176713/1‑135 (MQ=255) gAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTg > 6:205963/1‑135 (MQ=255) acgCCCGGGCGGCGCGGGCGCGCGCCGGGCTGAACCGCGGCTAGCCCCCCGGC‑CCCGCGCCGATCCCCGGCCGTAGCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTgg < 6:89739/133‑1 (MQ=255) cgCCCGGGCGGCGCGGGAGCGCGCCCGGCTTAACCTCGGCTAGGCCACCGGG‑CCCGCGCCGATCCCCGGCCGTACCGCCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGc < 3:240349/134‑1 (MQ=255) gCCAGGGCGGCGCTGGAGCGCGCCCGGCTGCACCTCGGCTATGCCCCCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCCGGTCCCCGACGGCGCGCGGGTCGGGCAAAACGAAACCACCCCGCTGGCg > 4:161847/1‑135 (MQ=39) ggggcgcgggcgcgCGCCCGGCTTACCCGCGGCTACCCCACCGTG‑CCCGCGCCGATCCCCGGCGGTAGCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATc < 1:114983/133‑1 (MQ=255) gcggcgCTGGCGCGCGCCCGGCTTAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATc < 6:118505/135‑1 (MQ=255) gcggcgCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCTCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATc < 6:291401/135‑1 (MQ=255) tGGAGCGCGCCCGGCTGCACCTCGGCTATGCCCCCGTG‑ACCGCGCCGAGCTCCGGCCGTATCGGCCGCGCCCAGGTCCCCGAAGGCGCGCTGGGCGGGCAAAACGCAACCACCCCGCTGGCGACCATCCAGCAGt > 6:159906/1‑135 (MQ=255) tGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGt > 6:208646/1‑135 (MQ=255) gtgcgggcCGGGCTGAACCGCGTGTATGCAACGGTT‑CCCGCGCCGAGCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGTGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTg < 3:116194/133‑1 (MQ=255) gAGCGCGCCGGGCTGAACCTCGGCTATGCCACCGGG‑CCCGCGCCGAGCGCCGGCCGTATCGCCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTg < 2:249274/135‑1 (MQ=255) gAGCGCGCCCGGCTGAACCTCGGCTATCCCACCGTT‑CCCGCGCCGATCTCCGGCCGTAGCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTg < 2:326196/135‑1 (MQ=255) gAGCGCGCCCGGCGGAACCTCGGCTATGCCACGGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTg < 8:190008/135‑1 (MQ=255) cgcgcgcCGGGCTCACCCTCGGATTGGCCCCGGGG‑CCGGCGCGGAGCTCGGGCGTTAGCGCCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTgg < 6:260286/134‑1 (MQ=255) aGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCCCCGAAGGCGCGCGGGTCGGGCAACACGCACCCACCCCGCTGGCGCCCATCCCGCAGTTgg > 2:76441/1‑135 (MQ=255) gggcgcCCGGCTTTACCTCCGCGATGCCACCGGGCCCCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTgg < 8:137051/133‑1 (MQ=255) gcgcCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCTCCGCAGGCGCGCTGGTCGGGCAAACCGACACCCCCCCGCTGGCGACCATCCCGCAGTTGGAcc > 5:250478/1‑135 (MQ=255) cgcCGGGCTCACCCGCGGCTAGGCCCCGGTG‑CCGGCGCCGAGCGCCGGCCGTAGCGCCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGAccc < 7:87949/135‑1 (MQ=255) ccGGCTGACCCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGACACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGAt > 8:39054/1‑135 (MQ=255) ccGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGCAACCACCCCGCTGGCGACCATCCCGCAGTTGGACCCgct > 7:221902/1‑133 (MQ=255) ccGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGCCCCTCCAGCAGTTGGCCCCGAt > 7:125442/1‑135 (MQ=255) ccGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCGGGTCGGGCAGAACGAACCCACCCCGCTGGCGCGCATCCAGCAGTTGGACCCgct > 1:323114/1‑133 (MQ=255) gCTTAACCTCGGCTATGCCACCGGG‑CCCGCGCCTATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCa < 5:52123/135‑1 (MQ=255) gCTGAACCTCGGCTATCCCCCCGGC‑CCCGCGCCGAGCCCCGGCGGATGCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCa < 2:69369/135‑1 (MQ=255) tGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCTGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCACAACGAAACCACCCCGCTGGCGACCCTCCAGCAGTTGGACCCCCTCCagg > 1:115110/1‑133 (MQ=255) tgggatATCCCACGGTG‑CCGGCGCCGATCTCGGCCGGTAGCGCCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATg < 6:297918/130‑1 (MQ=255) gcGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTAGGGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATg < 7:331674/134‑1 (MQ=255) ggCTTTGCCACCGGG‑CCCGCGCCGATCTCCGGCCGTAGCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATGTc < 4:55235/135‑1 (MQ=255) ggCTATGCCACCGTG‑ACCGCGCCTATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATGTc < 4:255973/135‑1 (MQ=255) ggCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCCCCCCGCTGGCGACCAGCCCGCAGGTGGCCCCGATCCATGCCGAGGTc > 1:275617/1‑135 (MQ=38) ggCTATGCCACCGTG‑ACCCCGCCCATCACCGGCCGTATGGCCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATGTc < 5:156220/135‑1 (MQ=255) gccTATCCCACCGTG‑CCCGCGCCGAGCCCCGGCCGTAGCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATGTc < 1:224579/133‑1 (MQ=255) cTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGCAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCAGGCCGATGTccc > 2:202399/1‑133 (MQ=255) cTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAACACGAACCCACCCCGCTGGCGACCCTCCAGCAGTTGGACCCGATCCATGCCGATGTCAc > 8:264668/1‑135 (MQ=255) aTGCCACCGTG‑ACCGCGCCGCTCTCCGGCCGTATCGGCCGCGCCCCGGTCCCCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCCTGCCGCTGTCAccc > 1:198293/1‑135 (MQ=255) aTGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATAGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATGTCAc > 3:345197/1‑133 (MQ=255) ccACCGGG‑CCCGCCCCTATCTCCGGCGGTAGGGCCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATGTCACCCAGt < 5:113544/135‑1 (MQ=255) cACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATGTCACCCAGt > 8:139078/1‑134 (MQ=255) cACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATGTCACCCAGt > 2:283206/1‑134 (MQ=255) tG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGACACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATGTCACCCAGTCTAccc > 1:157887/1‑135 (MQ=255) | TGGTCGACGACAAAGCCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTG‑ACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGCAAAACGAAACCACCCCGCTGGCGACCATCCAGCAGTTGGACCCGATCCATGCCGATGTCACCCAGTCTACCC > KT2440/3909071‑3909337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |