Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,934,892 A→C 19.3% V100G (GTG→GGG)  CJ019_03719 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,934,8920AC19.3% 111.4 / 2.3 58V100G (GTG→GGG) CJ019_03719hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (3/42);  new base C (10/3);  total (13/45)
Fisher's exact test for biased strand distribution p-value = 1.31e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCACCGTAG  >  KT2440/3934763‑3935023
                                                                                                                                   |                                                                                                                                   
tgctgACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCcccccccccccccc                                                                                                                                >  8:126309/1‑135 (MQ=255)
tgctgACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACcccc                                                                                                                                >  2:247199/1‑135 (MQ=255)
          aaTCAGCAGCAGCACCCCCAGGTTTGTAAAGCCGCCGTGGG‑CCTTACCATTCAGCGACGCGGCGGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGGCGGCCACGCCGCCCGCCACACCCTCGggcggcg                                                                                                                      <  6:317008/135‑1 (MQ=255)
          aaTCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGGCCCCCCCCCCCCCCCCgggggggg                                                                                                                      >  5:271741/1‑133 (MQ=255)
             cagcagcagCACCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGGCCCCCGCCCCCCCCCCCggcgggggcc                                                                                                                   >  5:31520/1‑134 (MQ=255)
             cagcagcagCACCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCCCCCCCCCGggcggcggcc                                                                                                                   >  7:126534/1‑134 (MQ=255)
             cagcagcagCACCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCCCACCCGCGggcggcggcc                                                                                                                   >  8:99039/1‑134 (MQ=255)
             cagcagcagCACCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCCCACCCCCGggcgggcgcc                                                                                                                   >  8:198677/1‑130 (MQ=255)
              agcagcagcACCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCGGTGGCACCGCGCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCAc                                                                                                                  <  5:184991/135‑1 (MQ=255)
               gcagcagcaCCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCGCCCCCCCGCGGGCGGCGGcccc                                                                                                                 >  1:76883/1‑135 (MQ=255)
                 agcagcaCCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAg                                                                                                               <  7:126309/135‑1 (MQ=255)
                    cccaaccccCGGTTTGTTAACCCGGCGGTGGCCCTCACCATTCGGCGAAGCGGC‑GCCACCCCCCCCCCCTCCCTGTGCCCCCGCTATTGATGGCGCCCCGGCGCCCCCCCCCACCCGCGGGCGGCGGCACCAGCt                                                                                                             <  2:354173/130‑1 (MQ=255)
                    agcaCCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCAA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGACACGCCCCCCGCCACACCCACCGGCGGCCGCACCAGcat                                                                                                            >  8:250127/1‑133 (MQ=255)
                                         ccgccgTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCCCCCCCGCGGGCGGCGGGCCCCGCGGCCCGGCCTCGCGGCGCGCggg                                                                                       >  6:294632/1‑134 (MQ=255)
                                          ctccgTTTG‑CATTACCATTCACCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCCCCGCTCTTTATGGCGGCCACGCCGCCCGCAACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTg                                                                                      <  4:290871/133‑1 (MQ=255)
                                               ttGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCgg                                                                                 <  7:99039/135‑1 (MQ=255)
                                               ttGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCCCCACCGCGGGCCGCGGGCCCAGCGGGCCGGCATAGCAGCCCGCGGTGTTCgg                                                                                 >  4:182890/1‑135 (MQ=255)
                                                    ccTTACCATTCAGCGAAGCGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCGCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCg                                                                             <  6:267358/135‑1 (MQ=255)
                                                     cTTACCATTCAGCTACGCGGCTGCCA‑GCCCGCCCCCGCCGTGGGCCCCCGCGCTTGATGGCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGa                                                                            <  5:2439/135‑1 (MQ=255)
                                                     ataaCCATTCACGGATGCGGAGGCCA‑TCACGCGCCCACACTGTGGAACCGCTGTTTTTGTCGGCCACCCGGCCCGCAACACCGTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGa                                                                            <  2:203133/132‑1 (MQ=255)
                                                             tCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGgtgtg                                                                    <  7:198677/135‑1 (MQ=255)
                                                               cccGATGAGGCGCCCA‑CCACGCGCCCCCCTGGGGGCCCCGCGTTTGATGGCGCCCCCGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGgtgtgt                                                                   <  8:334501/132‑1 (MQ=255)
                                                               aGCGAAGTGGCTGCCA‑TCCCGCGCCCTCCCTGGGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGgtgtgt                                                                   <  3:19109/134‑1 (MQ=255)
                                                               aGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGgtgtgt                                                                   <  3:161222/134‑1 (MQ=255)
                                                                   aaGAGGCTGCCA‑GCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTgcgc                                                              <  4:48582/135‑1 (MQ=255)
                                                                       ggCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCACGGGCGGCGGCCCCAGCTGCCAGGCAAAGCTGCGGACGGGGATCGGGTCGACCGGTGTGTTGGGCAgg                                                           >  2:315956/1‑134 (MQ=255)
                                                                       ggCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCCCCCCCCCCACCCCAGGGCGGCGGGCCCCCCGGGCAGGCCACGCGGCGCGCGGTGATAGGGTGGCCCGGGGTGTTGCGCAgg                                                           >  6:342170/1‑134 (MQ=255)
                                                                         cTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTaa                                                        <  7:271588/135‑1 (MQ=255)
                                                                                 cACGCGCCCTCCCTGTGGCACCGCGCTTGATGGCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACgttgtt                                                 <  4:338702/135‑1 (MQ=255)
                                                                                              tgtgGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTc                                    <  8:239961/135‑1 (MQ=255)
                                                                                              ggtgGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTc                                    <  2:74440/134‑1 (MQ=255)
                                                                                                 ggCACCGCTCTTGATTTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCa                                  <  1:72595/134‑1 (MQ=255)
                                                                                                  gCACGGCTGTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGGGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGc                                <  8:257480/135‑1 (MQ=255)
                                                                                                            tGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCggg                        <  1:72762/133‑1 (MQ=255)
                                                                                                              aTGGCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTa                     <  5:294632/134‑1 (MQ=255)
                                                                                                               tggcGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCTGGGTAGt                   <  6:284259/132‑1 (MQ=255)
                                                                                                               tGTCGGCCCCGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGt                   <  3:182890/135‑1 (MQ=255)
                                                                                                               tGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGt                   <  2:70736/135‑1 (MQ=255)
                                                                                                                ggcGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGt                   <  5:342170/132‑1 (MQ=255)
                                                                                                                    gCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCg              <  2:287911/135‑1 (MQ=255)
                                                                                                                     ccccgccgcCCCCCCCACCCGGGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  5:229149/132‑1 (MQ=255)
                                                                                                                     ccccgccgcCCCCCACACCGTGGGGGGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  5:29889/132‑1 (MQ=255)
                                                                                                                     cccccccgcccgcCACCCCCGCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  5:368748/130‑1 (MQ=255)
                                                                                                                     ccACGCCGCCCGCCCCACCCGGGGGGGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  5:17033/135‑1 (MQ=255)
                                                                                                                     ccACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  1:296129/135‑1 (MQ=255)
                                                                                                                     ccACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  6:113897/135‑1 (MQ=255)
                                                                                                                     ccACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  7:41175/135‑1 (MQ=255)
                                                                                                                     ccACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  7:250127/135‑1 (MQ=255)
                                                                                                                     ccACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGGCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCgg             <  5:100345/135‑1 (MQ=255)
                                                                                                                     ccACGCCGCCCCCCCCCCCCGAGGGCGGCGGCACCAGCTGCCACGCCTAGCAGCGCTCGGTGGTCGGGGCGGCCGGTGTGTTTGGCCGGTTACGTTGTTCCACCACGTGTTTCAGCGAGTCGGGGGAGTGGGCgg             >  2:59915/1‑135 (MQ=255)
                                                                                                                       aCGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTa           <  6:31520/135‑1 (MQ=255)
                                                                                                                        cgccgcCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTAt          <  2:318008/135‑1 (MQ=255)
                                                                                                                          ccgcccgcCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCa        <  5:362415/135‑1 (MQ=255)
                                                                                                                          ccgcccgcCACACCCGCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTGCCAGCGAGTCGGGGTAGGGGCCGGTATCa        <  2:364330/135‑1 (MQ=255)
                                                                                                                          ccgcccccCACCCCCGGGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGGCGGGGTAGTGGCCGGTATCa        <  1:165074/135‑1 (MQ=255)
                                                                                                                             cccgccACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCACCg     <  8:71990/135‑1 (MQ=255)
                                                                                                                                gccACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCACCgtag  <  2:89577/135‑1 (MQ=255)
                                                                                                                                gccACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCACCgtag  <  6:386779/135‑1 (MQ=255)
                                                                                                                                gccACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCACCgtag  <  8:47073/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                   
TGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGG‑CCTTACCATTCAGCGAAGAGGCTGCCA‑TCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAGGTAACGTTGTTCCACCAGTTGTTCCAGCGAGTCGGGGTAGTGGCCGGTATCACCGTAG  >  KT2440/3934763‑3935023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑