Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,029,454 A→C 23.4% D287A (GAC→GCC)  mcpU_3 → Methyl‑accepting chemotaxis protein McpU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,029,4540AC23.4% 163.9 / 6.1 109D287A (GAC→GCC) mcpU_3Methyl‑accepting chemotaxis protein McpU
Reads supporting (aligned to +/- strand):  ref base A (32/47);  new base C (18/11);  total (51/58)
Fisher's exact test for biased strand distribution p-value = 5.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

AGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCTGGG  >  KT2440/4029324‑4029585
                                                                                                                                  |                                                                                                                                   
   cTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTa                                                                                                                              <  7:136431/135‑1 (MQ=255)
     gCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCCAGCTGGCCCCGCGCTTCGCCCAGGTAGa                                                                                                                            >  4:351452/1‑135 (MQ=255)
       aGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCCAGCTGGCCCAGCGCGTCGACCAGGTAGAcc                                                                                                                          >  2:29464/1‑135 (MQ=255)
       aGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCGTCGACCAGGGAGAcc                                                                                                                          >  7:319331/1‑135 (MQ=255)
       aGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGCCCAGGTAGAcc                                                                                                                          >  7:25447/1‑135 (MQ=255)
       aGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCGGGCCCCGCGCGTCGACCAGGTAGccc                                                                                                                          >  5:89563/1‑135 (MQ=255)
         gCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGCCCCg                                                                                                                        >  7:376386/1‑135 (MQ=255)
         gCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTCGCTACAGCGCCGATGCCAGCAAACTGGCCCCGCGCATCGCCCAGGTAGcccct                                                                                                                        >  8:103230/1‑134 (MQ=255)
          cACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGGAGACCCGa                                                                                                                       >  8:154896/1‑135 (MQ=255)
          cACTGAGCCACGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGAAGGCTACCTCGCCGATGCCAGCAAGCAGGCCCAGCGCGTCGACGCGGTAGAACCGa                                                                                                                       >  7:275047/1‑135 (MQ=255)
              gAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATTCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGAcc                                                                                                                     >  8:317302/1‑133 (MQ=255)
                gCCAGGAGGCCAGCCGCAGCCTCTACGACGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAg                                                                                                                 <  4:384467/135‑1 (MQ=255)
                 ccAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTTCAGCGCCGATGCCAGCAAGCTTGCCCAGCGCTTCGCCCAGGTAGACCCGCCCaaacg                                                                                                                >  1:242244/1‑133 (MQ=255)
                      aggCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGGCGGGCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCg                                                                                                           <  7:117347/135‑1 (MQ=255)
                       ggCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCTTCGACCACGTAGCCCCGACCAAACGCGCCGa                                                                                                          >  8:136085/1‑135 (MQ=255)
                       ggCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGCCCCGACCAAAGGCGCCGa                                                                                                          >  4:125047/1‑135 (MQ=255)
                       ggCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGa                                                                                                          <  8:223977/135‑1 (MQ=255)
                        gCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTTTTGGCTGGCTACAGCGCCGATGACCGCAAGCGGGCCCAGCGGATCGCCCCGGGAGACCCGACCAAACGCGCCgcg                                                                                                         >  1:280635/1‑133 (MQ=255)
                        gCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCAAGCTGGCCCCGCGCGTCGCCCAGGTAGCCCCGACCCAAGGCGCCgcg                                                                                                         >  7:220366/1‑133 (MQ=255)
                        gCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAg                                                                                                         >  1:207760/1‑135 (MQ=255)
                          cAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCCAGCTGGCCCAGCCCGTCGCCCAGGTAGACCCGCCCAAAGGCGCCGCGCt                                                                                                       >  6:82661/1‑135 (MQ=255)
                            gCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGCCCAGGTAGACCCGACCAAAGGCGCCGAGCTgg                                                                                                     >  3:349749/1‑135 (MQ=255)
                            gCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCACCGCTGCCAGCAAGCTGGCCCAGCGCGTCGCCCAGGTAGACCCGACCAAAGGCGCCGCGCggg                                                                                                     >  1:318408/1‑135 (MQ=255)
                             ccGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCCCCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCCAGCTGGCCCCGCGCGTCGACCCGGGAGACCCGACCAAAGGCGCCGCGCTGGt                                                                                                    >  4:230078/1‑135 (MQ=255)
                             ccGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGCTGCCAGCCAGCTGGCCCAGCGCTTCGCCCCGGTAGCCCCGACCAAAGGCGCCGCGCTGGt                                                                                                    >  1:243073/1‑135 (MQ=255)
                             ccGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGCTGCCAGCAAGCTGGCCCCGCGCGTGGACCCGGTAGACCCGCCCCAACGCGCCCAGCgggg                                                                                                    >  3:38544/1‑134 (MQ=255)
                             ccGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCCACCAGGGCCAGCGCGTAGCCCCGGTAGACCCGCCCAAAGGCGCCGAGCGGGt                                                                                                    >  3:267345/1‑135 (MQ=255)
                             ccGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGt                                                                                                    <  8:195379/135‑1 (MQ=255)
                             ccGCAGCCTCAACGAAGGCCGCACCACGCTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGt                                                                                                    <  8:312324/135‑1 (MQ=255)
                              cGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCCAGCTGGCCCAGCGCTTCGACCCGGTAGCCCCGACCAAAGGCGCCGCGCTGGTg                                                                                                   >  4:380194/1‑135 (MQ=255)
                              cGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCCGTCGGTCTGTTGGCTGGCTACAGCGCCGAAGCCACCCAGCTGGCCCCGCGCGTGGCCCCGGGGGAGCCGCCCAAACGCGCCCCGCGGGTg                                                                                                   >  5:117004/1‑135 (MQ=255)
                                 aGCCTCTACGACGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGGCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCGGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTgcgc                                                                                                <  5:311381/135‑1 (MQ=255)
                                          gacggCCGCACCACGGTCGTCATTCTCACCCCGGTCGGTCTGTTGGCGGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTgg                                                                                         <  4:267345/130‑1 (MQ=255)
                                             ggacGCACCTCGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGTTAGACCCCACCAAAGGCGCCTAGCTTGTGCGCAAGCTGGCCGAt                                                                                    <  3:325765/132‑1 (MQ=255)
                                             ggCCGCACCACGGGCGGCATTCTCACCCCGGTCGGGCGGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGAt                                                                                    <  8:172848/135‑1 (MQ=255)
                                                cGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCGTGGCCCCGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGCTGGc                                                                                 >  5:191738/1‑135 (MQ=255)
                                                    ccacGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCCAGCTGGCCCAGCGCGTCGCCCAGGTAGCCCCGGCCAAAGGCGCCGCGCTGGTGCGCAAGCTGGCCGCTGGcccgc                                                                             >  7:279365/1‑131 (MQ=255)
                                                    ccacGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGCCCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCCAGCTGGCCGATGGCAAGc                                                                             >  8:128464/1‑135 (MQ=255)
                                                     cacGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCt                                                                            <  1:243961/135‑1 (MQ=255)
                                                       cGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGa                                                                          <  4:363306/135‑1 (MQ=255)
                                                           cGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGCTGCCCGCAAGCGGGCCCAGCGCGTCGCCCAGGTAGACCCCACCAAAGGCGCCGCGCGGGTGCGCAAGCTGGCCGATGGCAAGCTGaccac                                                                      >  3:183496/1‑135 (MQ=255)
                                                            ggCATTCTCACCCCGGTCCGTCTGTTGGCTGGCTACAGCGCCGATGCCAACAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGaccac                                                                      >  1:90033/1‑134 (MQ=255)
                                                                  ctcACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCAAGCTGGCCCAGCGCTTCCACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGGGCGCAAGCTGGCCGCTGGCCAGCGGCCCCCCCAcccc                                                               >  7:172838/1‑133 (MQ=255)
                                                                       cgcggtcggtCTGTTGGCTGGCTACCGCGCCGTGGCCCGCAAGCTGGCCCTGCGATTCGACCAGGTAGACCCGACCAACGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCg                                                          <  2:237922/133‑1 (MQ=255)
                                                                       cccGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCg                                                          <  2:80125/135‑1 (MQ=255)
                                                                       cccGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCg                                                          <  4:181784/135‑1 (MQ=255)
                                                                              ggtcTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGCCCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAgcgc                                                   >  8:284512/1‑135 (MQ=255)
                                                                               gtcTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCt                                                  >  8:116615/1‑135 (MQ=255)
                                                                               gtcTGTTGGCGGTCTACAGCGCCGATCCCCGCAAGCGGGCCCAGCGATTCGAACAGTTAGACCCGACCAACGGCGCCGAGCTGTTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCCCCACGACCGGCAGCGCt                                                  <  2:307715/135‑1 (MQ=255)
                                                                               gtcTGTGGGGTGGAAACAGCGCCTAGGCCCGCAAGCGGGCCCAGCGCTTCGCCCAGTTAGACCCGACCAACGGCGCCGAGCTGGTGCGCAAGCGGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCt                                                  <  4:38544/135‑1 (MQ=255)
                                                                                  tGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAgcgcgt                                                 >  8:110400/1‑131 (MQ=255)
                                                                                         tggctACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGCCCCGCCCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCt                                        >  1:42447/1‑135 (MQ=255)
                                                                                          ggctACCGCGCCGATGCCCGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGGGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTg                                       <  7:314342/135‑1 (MQ=255)
                                                                                            ctACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTg                                       <  4:100640/133‑1 (MQ=255)
                                                                                             tACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGCCCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTggcg                                    >  3:227653/1‑135 (MQ=255)
                                                                                                          ccagccagCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCCGCGCTTGAAGGTGCGGGCGGCATTCCGGCCaa                       >  5:46733/1‑135 (MQ=255)
                                                                                                          ccAGCAAGCTGGCCCCGCGCGTCGGCCCGGTAGCCCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACACCACGCCACGCCCGGCGGCGAGTGAAGGTGCTGGCGGCATTCCTGCCaa                       >  8:280792/1‑135 (MQ=255)
                                                                                                          ccAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGCCCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCGGACCACCCTCCCCGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCaa                       >  5:249965/1‑135 (MQ=255)
                                                                                                          ccAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGCTGGCACGCTGACGACCCTCCACGACCGGCAGCGCGTGAAGGTGCTGGCGGCATTCCGGCCaa                       >  2:41829/1‑135 (MQ=255)
                                                                                                          ccAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGCCCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCaa                       >  3:228464/1‑135 (MQ=255)
                                                                                                             gCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATcc                    <  8:205815/135‑1 (MQ=255)
                                                                                                             gCAAGCTGGCCCAGCGCTTCGACAAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATcc                    <  4:375519/135‑1 (MQ=255)
                                                                                                             gCAAGCTGCCCCCGCGCTACGCCCTGGTCGACCCGCCCAACGGCGCCGAGCGGGGGCGCAAGCTGGCCGAGGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATcc                    <  1:131891/135‑1 (MQ=255)
                                                                                                             gCAAGCGGGCCCCGCGCTTCGACCAGTTAGACCCGACCAACGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATcc                    <  2:152861/135‑1 (MQ=255)
                                                                                                                 gCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGa                >  3:17324/1‑135 (MQ=255)
                                                                                                                  gtGGCCCAGGGCGTCGACCTGGTACCCCCGAACAACGGCGCCGTGTTGGTGCTCATGCTGGCCGATGGCAAGCTGACCAACCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  3:222913/134‑1 (MQ=255)
                                                                                                                  gtGGCCCAGCGCTTCGACCAGGTAGACCAGACCAAAGGCGCCTAGCTGGTGCGAAAGCTGGCCTATGGCAAGCTTACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  4:320873/134‑1 (MQ=255)
                                                                                                                  ggggCCCCGCGCTTCGCCCCGTTCCACCCCCCAAACGGCGCCGAGCGGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  1:103014/133‑1 (MQ=255)
                                                                                                                  ggggCCCAGGGCTTCGCCCTGGTTGCCCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCGGGCCGATGGCAAGGTGACCACCCTCCACGACCGGCTGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  8:269934/133‑1 (MQ=255)
                                                                                                                  cgggCCCTGCGCTTCGACCTGGTACACCCGACAAAACGCGCCGAGCTGTGGCGCAAGGGGGCGGATGGCAAGCGGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGTCCAATCCCGGAc               <  7:307674/133‑1 (MQ=255)
                                                                                                                  cgggCCCCGCGTTGCCACCAGTCAGCCCCGACCACAGGCGCCGAGCTGGTGCGCAAGCGGGCCGTTGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  5:389263/133‑1 (MQ=255)
                                                                                                                  cgggCCCAGCGCTTCGCCCAGTTAGACCCGACCAAAGGCGCCGAGCGGGTGCGCAAGCTGGCCTATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  4:303253/133‑1 (MQ=255)
                                                                                                                  cgggCCCAGCGCTTCGCCCAGGACCCCCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  1:205340/133‑1 (MQ=255)
                                                                                                                  cgggCCCAGCGCTTCGACCAGGTACACCCGACAAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCCCCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  3:113659/133‑1 (MQ=255)
                                                                                                                  cTGGCCCAGCGGTTCGCCCCGGTCGACCCGACCAACGGCGCCGAGGTGGTGCGCAAGCTGGCCGATGGAAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  3:390459/135‑1 (MQ=255)
                                                                                                                  cTGGCCCAGCGCTTCGACCAGGTAGACCGGACCAACGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  2:77764/135‑1 (MQ=255)
                                                                                                                  cTGGCCCAGCGCTTCGACCAGGTAGACCCGCCAAACGGCGCCGAGCTGGTGCGCAAGCGGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  1:263509/135‑1 (MQ=255)
                                                                                                                  cTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAACGGCGCCGAGCTGGTGCGCAAGCGGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  8:195327/135‑1 (MQ=255)
                                                                                                                  cTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  8:25447/135‑1 (MQ=255)
                                                                                                                  cTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  8:376386/135‑1 (MQ=255)
                                                                                                                  cTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  8:63847/135‑1 (MQ=255)
                                                                                                                  cTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  6:90477/135‑1 (MQ=255)
                                                                                                                  cTGGCCCAGCGCTTCGACAAGTTGCACCCGACCAACGGCGCCGAGCTGGTGCGCAAGCTGGCCTATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  8:319331/135‑1 (MQ=255)
                                                                                                                   tGGCCCTGCGCTTCGACCTGTTAGACCCTACAAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  2:77767/134‑1 (MQ=255)
                                                                                                                   tGGCCCAGCGCTGCGCCCCGGTAGACCCGCCCAACGGCGCCGAGCGGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  3:125047/134‑1 (MQ=255)
                                                                                                                   gggCCCCGCGCTGCCCCCAGGTAGACCCGACCAACGGCGCCGAGCGGGGGCGCAAGCTGGCCGATGGCAAGCTGACCACCCCCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  2:318408/133‑1 (MQ=255)
                                                                                                                   gggCCCAGCGCTTCGCCCCGGTAGACCCCACCAACGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGAAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  1:235848/133‑1 (MQ=255)
                                                                                                                   gggCCCAGCGCTGCGACCTGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGGTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  2:242244/133‑1 (MQ=255)
                                                                                                                      cccAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGCCCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGCCCAAGCCCGGACGc             >  7:269934/1‑133 (MQ=255)
                                                                                                                        cAGCGCTTCGACCAGGTAGCCCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  5:361373/129‑1 (MQ=255)
                                                                                                                        cAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               >  6:361373/1‑129 (MQ=255)
                                                                                                                        cAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               <  4:67871/129‑1 (MQ=255)
                                                                                                                        cAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc               >  3:67871/1‑129 (MQ=255)
                                                                                                                         aGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAg         >  6:137469/1‑134 (MQ=255)
                                                                                                                          gcgcTTCTACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGcc       <  8:207264/135‑1 (MQ=255)
                                                                                                                            gcTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCt     <  4:227653/135‑1 (MQ=255)
                                                                                                                            gcTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCt     <  7:116615/135‑1 (MQ=255)
                                                                                                                              ttCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGCCCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCTgg   >  6:258928/1‑135 (MQ=255)
                                                                                                                               tCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCTACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCTggg  <  3:181662/135‑1 (MQ=255)
                                                                                                                               tCGACCAGGTAGACCCGACCAAAGGCGCCGAGCGGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCTggg  <  8:305652/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                   
AGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAGCCCTGGG  >  KT2440/4029324‑4029585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: