Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,080,182 T→G 23.3% R412R (CGT→CGG)  dadA → D‑amino acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,080,1820TG23.3% 148.6 / 12.4 80R412R (CGT→CGG) dadAD‑amino acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (13/45);  new base G (19/2);  total (33/47)
Fisher's exact test for biased strand distribution p-value = 5.19e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGTGGTTCAACTTCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACTATGGCGCGTTCCAC  >  KT2440/4080056‑4080315
                                                                                                                              |                                                                                                                                     
gCTGTGGTTCAACTTCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTg                                                                                                                               <  2:377054/135‑1 (MQ=255)
     ggTTCAACTTCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAAc                                                                                                                            <  3:124310/133‑1 (MQ=255)
     ggTTCAACTTCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAAc                                                                                                                            <  7:1545/133‑1 (MQ=255)
       ttCAACTTCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGCGCCCCCCTTCACCGCCCCCGCCCCGTCCAGCGCCCCCCGGGTGGACGGAAccccc                                                                                                                        >  5:274973/1‑133 (MQ=255)
       ttCAACTTCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCCCCGACCCCCCGCCGTACACCCCGCCCCCGTTAGCCTGCAccccc                                                                                                                        >  6:339192/1‑133 (MQ=255)
       ttCAACTTCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTGACAGCGCGCCCCCGTTGGACTGCAccccc                                                                                                                        >  7:61634/1‑133 (MQ=255)
       ttCAACTTCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCGTACCGCGCGCCCCGTTTCGACTGCACCCAc                                                                                                                        >  4:124310/1‑135 (MQ=255)
       ttCAACTTCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCCCCGTACCCCGCGACCCGTTTCGACGGCACCCAc                                                                                                                        >  8:113959/1‑135 (MQ=255)
       ttCAACTTCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCCCGGCGGACAGCGCGCCCCGGGTCGCCGGAACCCAc                                                                                                                        >  3:372444/1‑135 (MQ=255)
       ttCAACTTCGGCCATGCCCATCACGGCCCCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCGTTCAGCGCGCCCCGGTTCGACTGCAccccc                                                                                                                        >  8:135779/1‑133 (MQ=255)
              tCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCCTACAGCGGGCCCCGGGTCGACTGAACCCACGCgggg                                                                                                                  >  7:258167/1‑133 (MQ=255)
                ggCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGgag                                                                                                                 <  1:180435/133‑1 (MQ=255)
                  ccATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCCCTGGCGAGCCCCCCTTCACAGACCCCGCGCCGTACCGCGCCCCCCCATTCGCCTGACCCCCCCCGGgagcccc                                                                                                             >  7:178008/1‑131 (MQ=255)
                      gCCCATCACGGCCTCACGCTGGGCGCGGTGTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCGTTACAGCGGGACCCGTTTCGACTGAACCCAGGCGGGAGAAcaacaa                                                                                                         <  5:235676/135‑1 (MQ=255)
                      gCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAAcaacaa                                                                                                         <  6:368637/135‑1 (MQ=255)
                           tCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGa                                                                                                    <  5:47118/135‑1 (MQ=255)
                           tCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGa                                                                                                    <  8:146633/135‑1 (MQ=255)
                           tCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGa                                                                                                    <  7:319464/135‑1 (MQ=255)
                           tCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGa                                                                                                    <  1:702/135‑1 (MQ=255)
                           tCACGGCCTCACGCTGGACCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGa                                                                                                    <  1:230757/135‑1 (MQ=255)
                            cACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGAc                                                                                                   <  3:22888/135‑1 (MQ=255)
                                 ccTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGcac                                                                                              <  4:277260/135‑1 (MQ=255)
                                    cACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCGTACAGCGCGCCCCGGTTCGACTGAACCCCCGCGGGAGAACAACAAGATGACCGcaccac                                                                                           >  3:366541/1‑135 (MQ=255)
                                    cACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCGTACAGCGCGCCCCGGGTCGGCTGCACCCCCGCGGGAGCACCACAACATGCCCGcccccc                                                                                           >  1:4731/1‑133 (MQ=255)
                                    cACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCGTACAGCGCGACCCGGTTCGCCTGAACCCCCGCGGGGGCACAACAAGCTGACCGccccac                                                                                           >  5:87288/1‑135 (MQ=255)
                                    cACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCCCGCCGGCCCGCGCGCCCCGGGTCGGCGGGCCCCACGCCGGGGCCCAACAAGAAGGCCCccccac                                                                                           >  5:390597/1‑135 (MQ=255)
                                     aCGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACt                                                                                          <  4:10829/135‑1 (MQ=255)
                                     aCGCGGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACt                                                                                          <  6:279385/135‑1 (MQ=255)
                                      cGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTg                                                                                         <  3:362334/135‑1 (MQ=255)
                                       gCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACCCCGCGCCCCGGTGCGACGGCACCCCCGCGGGAGCCCCACCAGATGACCGCACCCCTGa                                                                                        >  2:105061/1‑135 (MQ=255)
                                            gCCCGGGCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCtt                                                                                   <  3:312770/135‑1 (MQ=255)
                                            gCCCGGGCTGCGGCCGGCGGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCtt                                                                                   <  7:335295/135‑1 (MQ=255)
                                              ccGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGCGCCCCCCTTCACCGACCCCGCGCCTTACCGCGCGCCCCGTTTCGACTGCTCCCACGCCGGAGAACACCAAGAAGACCGCCCCACTGCGCCGTgg                                                                                 >  4:57890/1‑134 (MQ=255)
                                              ccGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCGTACAGCGCGCCCCGGTTCGCCTGCACCCCCGCGCGAGAACAACAAGATGACCGCACCACAGAGCCGTGc                                                                                 >  8:287522/1‑135 (MQ=255)
                                              ccGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCACCCTTCACCGACCCCGCGCCCTAAAGCGCGGCCCGGGTGGACTGAACCCACCCGGGGGAACAACCAGATGTCCGCACCACCGAGCCATga                                                                                 >  8:23992/1‑134 (MQ=255)
                                              ccGGTCTGCGGCCGGCTGCTGGCCGAGCAGCTCACTGGCGAGCCCCCCTTCACAGACCCCGCGCCATAAAGCCAGACCCGGTTCGACAGAAACCACGCGGGACTACAACAAAATGACCGCCCCACATAGCCATg                                                                                  >  8:367448/1‑134 (MQ=255)
                                                 gTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGGGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCAc                                                                              <  6:347993/135‑1 (MQ=255)
                                                         ccggcTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGCCCCCGCGCCTTACCGCGCGGCCCGGTTGGCCTGACCCCCCGCGGGCGGACCACCAGCTGCCCGCCCCCCGGCGCCTGGCCCCCCGTGccc                                                                      >  5:166271/1‑135 (MQ=255)
                                                         ccggcTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGGCGTACAGCGCGCCCCCGTTCGACAGCACCCCCGGGGGTGACCAACAATCTGGCCGCACCACTGAcccatgccccccatgccc                                                                      >  7:43434/1‑135 (MQ=255)
                                                         ccggcTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCGTACAGCGCGACCCGTTTCGACTGAACCCCCGCGGGAGAACAACAAGATGCCCGCACCACGGAGCCTTGCCACCCTTGccc                                                                      >  2:35777/1‑135 (MQ=255)
                                                                  ggCCGAGCGGCTCACTGCCCCCCCCCCCTTCACCGCCCCCGCGCCGTACAGCGCGACCCGTTTCGACTGACCCCACGCGGTATAACAACACGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAacccg                                                             <  1:300776/135‑1 (MQ=255)
                                                                   gcggAGCTGCGCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCGGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAacccga                                                            <  8:178008/132‑1 (MQ=255)
                                                                   gCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAacccga                                                            <  4:82428/135‑1 (MQ=255)
                                                                        ggtgctCAGTGGGGCGCCCCCCTGCACCGACCCGGCGCGTTCCCGCGCGACCGGTTTCGACTGACCCCACGCGGGAGACCAACACGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCcgc                                                       <  5:307510/133‑1 (MQ=255)
                                                                        gctgctCCCTGGCTCGCCCCCCTTCACCTCCCCCGCGCCTTACGGCGGGACCCGTTTCTCCTGAACCACCGCTGGATACAACCAAGATGACCGCACCACTGCGCCTTGCCACCCTTGCCCCCGACCCCGACCcgc                                                       <  2:325464/135‑1 (MQ=255)
                                                                        gctgctCACTGGCGCGCCCCCCTGCCCCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGACCCCCCGCGGGAGAACAACACGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCcgc                                                       <  1:177807/135‑1 (MQ=255)
                                                                        gctgctCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACCGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCcgc                                                       <  1:56770/135‑1 (MQ=255)
                                                                        gctgctCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCcgc                                                       <  4:333365/135‑1 (MQ=255)
                                                                                   gcgcgcCCCCCTTCCCCCCCCCCGCGCCTTACCGCGCGACCCGTTTCGACTCAACCCCCGCGGGACAACAACAACATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTg                                            <  5:296028/135‑1 (MQ=255)
                                                                                    cGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTCAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCt                                             <  7:113959/133‑1 (MQ=255)
                                                                                         cccccTTCACCGACCCCGCGCCTTAAAGCGCGACCCGGTTCGAATGAAACCACGCGGGAGCAAAACAACATGACCGCACCACGGAGCCTTGCCACCCTTGCCCCCCAACCCCACCCGCGTCCGGTGCTgcatag                                       >  8:56459/1‑129 (MQ=255)
                                                                                          ccccTTCACCGCCCCCGCGCCTTACAGCGCGACCCGGTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGTACCCGACCCGCGTCCGGTGCTGAATCGCa                                     <  8:211109/135‑1 (MQ=255)
                                                                                                 aCCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACACGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGACCCCGACCCGCGTCCGGTGCTGATTCGCATCGAAg                               <  2:15697/134‑1 (MQ=255)
                                                                                                 aCCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAg                               <  8:101622/134‑1 (MQ=255)
                                                                                                   cGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGcc                            <  4:77640/135‑1 (MQ=255)
                                                                                                   cGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGcc                            <  7:30799/135‑1 (MQ=255)
                                                                                                     aCCCCGCGCCTTACAGCGCGACCCGTTTCGCCTGAACCCACGCGGGATAACAACATGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTg                          <  1:50883/135‑1 (MQ=255)
                                                                                                     aCCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTg                          <  4:390955/135‑1 (MQ=255)
                                                                                                        gcgcgCCTTACAGCGCGACCCGGTGCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCTAACCCGACCCGCGGCCGGTGCTGATTCGCATCGAAGGCCTGAAc                       <  3:140400/134‑1 (MQ=255)
                                                                                                        ccGCGCCTTACAGCGCGCCCCGGTTCGACTGAACCCACGCCGGAGAACAACAAGATGCCCGCCCCCCGGAGCCGTGGCACCCTTGCCCCCCAACCCCCTCCGCCTCCCGTGCGGAGTCGCATCGACGGccggca                        >  4:28034/1‑130 (MQ=255)
                                                                                                        ccGCGCCTTACAGCGCGACCGGTTTCGAATGAACCCAGGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAAc                       <  7:287522/135‑1 (MQ=255)
                                                                                                        ccGCGCCTTACAGCGCGACCCGTTTGGACTGAACCCCCGCGGGAGAACAACAAGATGACCGCACCACTGAGCCGTGCCACCCTTGCCCCCGAACCCGCCCCGCCTCCGGTGCTGATTCGCATCGAAGGCCTGaa                        >  2:48527/1‑134 (MQ=255)
                                                                                                        ccGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAAcaacaa                                                                                                         >  2:300733/1‑53 (MQ=255)
                                                                                                        ccGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAAcaacaa                                                                                                         <  1:300733/53‑1 (MQ=255)
                                                                                                        ccGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAAc                       <  3:383621/135‑1 (MQ=255)
                                                                                                        ccGCGCCTTACAGCGCGACCCGGTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACAGAGCCATGACACCCTTGCCCCCCAACCCGACCCGCCTCCCGTGCTGCTTAGCATCCAAGGCCTCaa                        >  2:4948/1‑134 (MQ=255)
                                                                                                          gcgcCTTACAGCGCGCCCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGCCCCGCGGCCGGTGCTGATTCGCATCGAAGGCCGGAACaa                     >  2:211714/1‑135 (MQ=255)
                                                                                                               ttACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACt                <  6:391339/135‑1 (MQ=255)
                                                                                                               ttACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACt                <  3:24706/135‑1 (MQ=255)
                                                                                                                   cgcgcGACCCGTTTCGACTTAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACTATgg            <  6:110724/134‑1 (MQ=255)
                                                                                                                   agagcgACCCGTTTCGAATGAACCCACGCGGGTGAACAACAATATGACCGCACCACTGAGCCTTGCCACCCTTTCCCCCGAACCCGACCCCCGTACCGTGCTGATTCGCATCGAACGCCTGAAAAAGCACAATgg            >  8:211948/4‑135 (MQ=255)
                                                                                                                   aGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACTATgg            <  5:353977/135‑1 (MQ=255)
                                                                                                                   aGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACTATgg            <  8:61634/135‑1 (MQ=255)
                                                                                                                     cgcgACCCGTTGCGACTGACCCCACGCGGGAGAACAACATGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACTATGgcg          <  4:995/135‑1 (MQ=255)
                                                                                                                        gACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACTATGGCGCGt       >  3:289412/1‑135 (MQ=255)
                                                                                                                        gACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACTATGGCGCGt       >  1:288364/1‑135 (MQ=255)
                                                                                                                        gACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGAACGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACTATGGCGCGt       >  5:158058/1‑135 (MQ=255)
                                                                                                                           ccGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCAGACCCGCGTCCGGTGCTGATTCCCATCGAAGGCCTGAACAAGCACTAAGGAGCGGTcc    >  3:995/1‑135 (MQ=255)
                                                                                                                             gTTTCGACTGAACCCACGGGGGAGAACAACTAGATGACCGCACCACTGAGCCGTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACTATGGCGCGTTCCAc  <  8:89527/135‑1 (MQ=255)
                                                                                                                             gTTTCGACTGAACCCACGCGGGAGAGCAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACTATGGCGCGTTCCAc  >  4:154027/1‑135 (MQ=255)
                                                                                                                              |                                                                                                                                     
GCTGTGGTTCAACTTCGGCCATGCCCATCACGGCCTCACGCTGGGCCCGGTCTGCGGCCGGCTGCTGGCCGAGCTGCTCACTGGCGAGCCCCCCTTCACCGACCCCGCGCCTTACAGCGCGACCCGTTTCGACTGAACCCACGCGGGAGAACAACAAGATGACCGCACCACTGAGCCTTGCCACCCTTGCCCCCGAACCCGACCCGCGTCCGGTGCTGATTCGCATCGAAGGCCTGAACAAGCACTATGGCGCGTTCCAC  >  KT2440/4080056‑4080315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑