Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,131,763 T→G 22.7% E23A (GAG→GCG)  CJ019_03889 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,131,7630TG22.7% 95.6 / 2.2 72E23A (GAG→GCG) CJ019_03889hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/29);  new base G (15/4);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 1.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACGATGGCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGC  >  KT2440/4131629‑4131897
                                                                                                                                      |                                                                                                                                       
gCACGATGGCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCt                                                                                                                                         >  8:386879/1‑135 (MQ=255)
    gATGGCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGc                                                                                                                                     <  4:25698/135‑1 (MQ=255)
     aTGGCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTGACCCTGGCGGGCGGCa                                                                                                                                    >  5:270812/1‑135 (MQ=255)
      tGGCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAg                                                                                                                                   <  3:294840/135‑1 (MQ=255)
        gCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGt                                                                                                                                 <  4:270633/135‑1 (MQ=255)
        gCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGt                                                                                                                                 <  2:102558/135‑1 (MQ=255)
         cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCCCCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCACCGGGCGCGCGGCCgggg                                                                                                                                >  2:306621/1‑133 (MQ=255)
         cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGt                                                                                                                                 <  2:56432/134‑1 (MQ=255)
         cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTg                                                                                                                                >  4:154304/1‑135 (MQ=255)
         cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCGCGGCAGGTg                                                                                                                                >  5:264970/1‑135 (MQ=255)
         cGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTACCAAGGCGCGAGGCAGGTg                                                                                                                                >  2:51826/1‑135 (MQ=255)
              aGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCCGGTGGAAc                                                                                                                            >  1:334067/1‑134 (MQ=255)
              aGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGt                                                                                                                                 <  8:68754/129‑1 (MQ=255)
              aGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGt                                                                                                                                 >  7:68754/1‑129 (MQ=255)
               ggCCCAACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGGCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGGCGCGCGCCTTCCCATGGCGCTCGGCAGGTGTAACGt                                                                                                                          <  3:284155/135‑1 (MQ=255)
                     acaacaGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCATCCCATGGCGCTCGGCCCGTGGCACCTAATcca                                                                                                                    >  2:51737/1‑133 (MQ=255)
                        acaGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCGCGGCAGGTGGAACGGAATCGCCCg                                                                                                                 >  3:177467/1‑135 (MQ=255)
                           ggTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCgtgt                                                                                                              <  7:297510/135‑1 (MQ=255)
                            gTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCGCGGCAGGTGGAACGGAATAGAACGtgtg                                                                                                             >  7:103292/1‑135 (MQ=255)
                               cAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCATGGAGCTCGGCAGGTGGGACGTAATCGCCCGTGTGAtt                                                                                                          >  8:103628/1‑135 (MQ=255)
                               cAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCCCCGTCCCCTGGCGCTCGGCAGGTGGCACGTAATCGCACGTGTGAtt                                                                                                          >  2:207591/1‑135 (MQ=255)
                                 ggTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGTCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCATGGCGCTAGGGATGTGGCACCTAATCGACCGTGTGCTtct                                                                                                        >  8:178114/1‑135 (MQ=255)
                                    ggAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGACCCAGAGGGTGAACAGGTCGCGCGCCGTCCCCTGGCGCGGGGCCGGTGGGACGTAATCGCCCGTGTGGTtctggg                                                                                                     >  8:205079/1‑132 (MQ=255)
                                        cacCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCATGGGGCTCGGCCGGTGGAACGTAATCGACCGTGTGCTTATCGACaaa                                                                                                 >  2:329872/1‑135 (MQ=255)
                                         acCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGTGTGAACAGGTCGCGCGCATTCCCAGGGCGGGCGGGAGGGGGAACGTAATCGACCGTGTCATTATCgcaac                                                                                                  >  2:235178/1‑129 (MQ=37)
                                          ccTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCt                                                                                                                                         <  8:102754/93‑1 (MQ=255)
                                          ccTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGCCCGTGGGATTCTCGACAAACt                                                                                               >  4:356085/1‑135 (MQ=255)
                                          ccTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCCCCTTCCCATGGCGCt                                                                                                                                         >  7:102754/1‑93 (MQ=255)
                                          ccTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCCCCGTGCCAAGGGCGTCGGCAGGGGGGACCTTATAGCCCCTGTGATTGTCGACAAAca                                                                                               >  3:325237/1‑134 (MQ=255)
                                              gACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATAGACCGTGTGGTTCTCGACAAACTGgtg                                                                                           >  6:156173/1‑135 (MQ=255)
                                                   ccttGGCGCCGGGCACGATAGGCAATGGCGCGATGTTGGAACTGAACCAGAGGGTCAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGcg                                                                                      <  1:225467/132‑1 (MQ=255)
                                                      tGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGGCGTGCCCTGGCGCGCGGCAGGGGGAACGTAATCGGCCGGGGGCTTGTCGTCCAACGGGTGTTgggccg                                                                                   >  4:24602/1‑130 (MQ=255)
                                                         ggcgggTCACGATAGGCTGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACTGGGCGGCCGCCTTCCATTGGCGCTCGGATGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCgacga‑c                                                                                <  3:288653/130‑1 (MQ=255)
                                                         cgcCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGTGTGAACATGTCGCGCGCCTTCCCCTGGCTCTCGGAAGGGGGAACGTACTCGACCGTGTGATTCTCGACAAACTGGTGTTGCgacga‑c                                                                                <  5:259040/134‑1 (MQ=255)
                                                              gTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGc                                                                                                                                     >  7:128159/1‑77 (MQ=255)
                                                              gTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGc                                                                                                                                     <  8:128159/77‑1 (MQ=255)
                                                              gTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTGCCCTGGCGCGCGGCAGGGGGCACGTAATCGCCCGTGTGCTTGTCGCCAAACTGGTGTTGGGCCGA‑CGGTTgg                                                                          >  6:145984/1‑135 (MQ=255)
                                                               tCACGATCTGCAGTGGCGCTATGTGGGTACTGAACCAGTGGGTGAACAGGTGGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAAGTTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTggg                                                                         <  1:149589/135‑1 (MQ=255)
                                                                 aCGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGAc                                                                       >  1:79121/1‑135 (MQ=255)
                                                                 aCGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTGCCCTGGCGCGCGGGCAGCGGAACGTAATCGCCCGTGGGATTCTCGCCAAACTGGTGTTGCGCCGC‑CCGGTgggc                                                                        >  7:10207/1‑133 (MQ=255)
                                                                 aCGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCCATGCGCGGGGCAGGTGGGACGGTATAGGCCGTGTTTTTGTCGACAAACTGGGGTTGGGACCACCGGTTggg                                                                         >  7:364678/1‑134 (MQ=255)
                                                                 aCGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCGTCCCATGGCGCGCGGCCGGTGGCACGGAATCGCCCGTGGGATTCTGGGCAAACTGGTGGTGCGACGG‑CGGGTGGGAc                                                                       >  5:134749/1‑135 (MQ=255)
                                                                                gcgATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAAAGTAATCGAACGTGTGATTCTAGACAAACATGTGTTGCGACGC‑CGGTTGGGACATGGTTTTGAGCTc                                                         >  6:656/1‑134 (MQ=255)
                                                                                  gATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGAAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGaa                                                      <  1:178584/135‑1 (MQ=255)
                                                                                       tGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTc                                                 >  4:191553/1‑135 (MQ=255)
                                                                                            gcgacccAGCGGGTTAACCGGGCGCGCGCCTTCCCATGGCGCTCGGCAGGGGGAAGGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGGGGGGACATGGTTTTCAGCTCGAAAGGTCGtttt                                            <  5:312325/130‑1 (MQ=255)
                                                                                                aCCAGAGGGTGAAGAGGTCGCGCGCGTTCCCATGGCGCGCGGCAGGGGGAAGGTAATCGACGGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTAt                                         <  5:204069/134‑1 (MQ=255)
                                                                                                aCCAGAGGGTGAACAGGGCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTAt                                         <  6:318217/134‑1 (MQ=255)
                                                                                                 ccAGAGGGTGAACAGGTCGCGCGCCTTCCCATTGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTATCGACAAACATGTGTTGCGACGC‑CGGTTGGGACATGGTTTTAAGCACGAAAGGTCGtttttttgt                                       >  7:11587/1‑131 (MQ=255)
                                                                                                 ccAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATAGACCGTGTGATTCTCGACAAAATGGTGTTTCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTAtat                                       >  3:344518/1‑133 (MQ=255)
                                                                                                 ccAGAGGGTGAACAGGTCGCGCGCCGTCCCATGGCGCTCGGCAGGTGGAACGTAATCGCCCGGGTGCTTCTCGACAAAATGGTGTTTCGCCGC‑CGGGTGGGGCATGGTTTTCAGCTCGAAAGGTCGtttttttat                                       >  3:335236/1‑131 (MQ=255)
                                                                                                 ccAGAGGGTGAACAGGTCGCGCGCCGTCCCATGGCGCGCGGCAGGTGGAACGTAATCTACCGTGTGTTTCTCGACAAACTGGTGTTGCGCCGA‑CGGTTGGGACATGGGTTTCAGCTCGCAAGGTCGtttttttat                                       >  4:69848/1‑131 (MQ=255)
                                                                                                  cAGCGGGTGAACAGGTCGCGCGCCTTCCAATGGCGCTGGGCAGGTGTAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGATATGGTTTTCAGCTCGAAAGGTCGTTTTTATCtt                                      <  7:178114/135‑1 (MQ=255)
                                                                                                         tGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAgg                               <  8:317642/135‑1 (MQ=255)
                                                                                                          gAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGGACGTAATCGACCGGGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGGTGGGACATGGTTTTCAGCTCGAAAGGTGGTTTTTTTCTTTGTAAgg                               >  4:260677/1‑134 (MQ=255)
                                                                                                          gAACAGGTCGCGCGCCATCCCCTGGCGCTCGGCAGGGGGAACGTAATAGACCGTGGGATTCTCGACAAACTGGTGTTGCGCCGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGGTTTTATCTTTGTTAgg                               >  7:44495/1‑134 (MQ=255)
                                                                                                          caaCAGGTCGCGCGCCTTCCCTTGGGGCTCGGCTGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGa                              <  6:275702/134‑1 (MQ=255)
                                                                                                            aCAGGTCGCGCGCCTTCCCATGGCGGTCGGCAGGTGGCACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGC‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGaaa                            >  7:52204/1‑135 (MQ=255)
                                                                                                            aCAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGaaa                            >  1:122265/1‑135 (MQ=255)
                                                                                                            aCAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGGGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATATTTTTAAGGaaa                            >  6:296421/1‑135 (MQ=255)
                                                                                                            aCAGGTCGCGCGCCTTCCCATGGCGCGCGGCAGGTGGCACGTAATCGCCCGTGTGATTCTCGACAAACTGGTGTTGCGCCGA‑CGGTTGGGCCATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGaaa                            >  1:53685/1‑135 (MQ=255)
                                                                                                              aGGGCGCGCGCCTTCCCATGGCGCGCGGCAGGTGTAACTTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAAcc                          <  1:83397/135‑1 (MQ=255)
                                                                                                                     gcgcCTTCCCATGGCGCTCGGCAGGTGTAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCaa                   <  2:129439/135‑1 (MQ=255)
                                                                                                                     gcgcCTTCCCATGGCGCTCGGCAGGTGTAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCaa                   <  6:270812/135‑1 (MQ=255)
                                                                                                                     gcgcCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCTACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCaa                   <  3:69848/135‑1 (MQ=255)
                                                                                                                     gcgcCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATGTTTGTAAGGAAACCGCATCaa                   >  4:327514/1‑135 (MQ=255)
                                                                                                                     gcgcCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCaa                   <  5:375734/135‑1 (MQ=255)
                                                                                                                     gcgcCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCaa                   <  8:266938/135‑1 (MQ=255)
                                                                                                                     gcgcCTTCCCATGGCGCTCGGCAGGGGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCaa                   <  1:51826/135‑1 (MQ=255)
                                                                                                                     gcgcCTTACCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCaa                   <  8:10207/135‑1 (MQ=255)
                                                                                                                     gcgcCTGCCCAGGGGGCGCGGCTGGTGGACCGTACTCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCaa                   <  2:175077/135‑1 (MQ=255)
                                                                                                                     ccgcCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGCCCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGGTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCaa                   <  6:176474/134‑1 (MQ=255)
                                                                                                                               aTGGCGCGCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCctct         <  7:103628/135‑1 (MQ=255)
                                                                                                                                   cgcTCGGCTGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCCCATCAAGGCGGCCTCTCAg      <  4:344518/134‑1 (MQ=255)
                                                                                                                                    gcTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAATGAAACCGCATCAAGGCGGCCTCTCAGCt    <  5:656/135‑1 (MQ=255)
                                                                                                                                      tCGGCTGGGGGAAGGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  4:325237/135‑1 (MQ=255)
                                                                                                                                      tCGGCAGGTTGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  1:306621/135‑1 (MQ=255)
                                                                                                                                      tCGGCAGGTGGACCGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  3:292200/135‑1 (MQ=255)
                                                                                                                                      tCGGCAGGTGGACCGTAATCGACCGTGTGAATCTCTACAAACTGGTGTTGCGACGA‑CGGTTGGGACAGGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAATGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  5:182447/135‑1 (MQ=255)
                                                                                                                                      tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGTCGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  3:144034/135‑1 (MQ=255)
                                                                                                                                      tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAATGAAACCGCATCAAGGCGGCCTCTCAGCTGc  >  8:349789/1‑135 (MQ=255)
                                                                                                                                      tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  7:386879/135‑1 (MQ=255)
                                                                                                                                      tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  6:264970/135‑1 (MQ=255)
                                                                                                                                      tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  2:122265/135‑1 (MQ=255)
                                                                                                                                      tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  1:207591/135‑1 (MQ=255)
                                                                                                                                      tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  3:154304/135‑1 (MQ=255)
                                                                                                                                      tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  <  4:145873/135‑1 (MQ=255)
                                                                                                                                      tCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAAATGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGc  >  3:193777/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GCACGATGGCGATCAGGCCCCACAACAGGTTCAGGTGGAACACCTGGACCACCATGGCGCCGGTCACGATAGGCAGTGGCGCGATGTTGGTACTGAACCAGAGGGTGAACAGGTCGCGCGCCTTCCCATGGCGCTCGGCAGGTGGAACGTAATCGACCGTGTGATTCTCGACAAACTGGTGTTGCGACGA‑CGGTTGGGACATGGTTTTCAGCTCGAAAGGTCGTTTTTATCTTTGTAAGGAAACCGCATCAAGGCGGCCTCTCAGCTGC  >  KT2440/4131629‑4131897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑