| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,135,592 | A→C | 11.9% | N27T (AAC→ACC) | tgnC → | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,135,592 | 0 | A | C | 11.9% | 174.1 / 3.1 | 83 | N27T (AAC→ACC) | tgnC | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (42/29); new base C (2/10); total (44/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAA > KT2440/4135458‑4135724 | tgGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCaa > 1:301669/1‑135 (MQ=255)tgGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCaa > 5:80500/1‑135 (MQ=255) gACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAAcc > 1:215528/1‑135 (MQ=255) gACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTCTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCACTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCGAcc > 2:284770/1‑135 (MQ=255) aCTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAAccc < 5:194300/135‑1 (MQ=255) ttCCTCACCCAAGCCCGCACCCTGACCGAATCCGCCAAGGGGATTGTTGCATGTCCCTCGTTCGTTTCCAGATGTGGATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 3:86732/135‑1 (MQ=255) ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 3:139103/135‑1 (MQ=255) ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 4:6706/135‑1 (MQ=255) ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 8:315386/135‑1 (MQ=255) ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 6:182087/135‑1 (MQ=255) ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 6:255683/135‑1 (MQ=255) ttCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 2:236628/135‑1 (MQ=255) cACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc > 8:54994/1‑135 (MQ=255) cACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc > 5:180592/1‑135 (MQ=255) cccAAGCCCGCACCCTCACCGACTCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc < 7:316311/133‑1 (MQ=255) cccAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCAcc < 6:43057/133‑1 (MQ=255) cccAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGc > 7:91963/1‑135 (MQ=255) cgaccgcTCACCGAATCCGCCACGGGGATTGTTGATTGACCGTCGTTCGTTTCCAGTTGTGCATCGTCGGCCAATGGCGCGATGCCCAGAGTGGCAAGACCTTCGAAAGCCTCAACCCGTCCACCTCCCAGGCCt < 8:357915/129‑1 (MQ=255) cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCCCCGCCCCGGccg > 1:119112/1‑134 (MQ=255) cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGccg > 7:122408/1‑134 (MQ=255) cGCACCCTCACCGAATCCGACAAGGTGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCAACGACGGCCAATGGCGCGATGCCCAGAGCGGCACCCCATTCGACAGACTCACCCCGGCCACCGCCCCGGcca > 1:337754/1‑134 (MQ=255) cGCACCCTCACCGAATACGCCAAGGGGATTGTTGCATGACCCTCTTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCAGccca > 6:236111/1‑134 (MQ=255) cACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGATCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGACTgg > 1:54885/1‑135 (MQ=255) aCCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGCCCTTCGACAGCCTCAACCCGGCCACCGCCCAGcccgcgg > 3:323596/1‑131 (MQ=255) ccTCACCGAATCCGCCAAGGGTTTTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCAGCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGgcg < 8:7222/135‑1 (MQ=255) ccTCACCGAATCCGCCAAGGGGATTGTTGCATGCCCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACCGCCTCAACCCGGCCACCGCCCAGGCCTGGgcg > 6:189634/1‑135 (MQ=255) ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCAGGgcg > 2:184824/1‑135 (MQ=255) cTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGCCCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGgcgc > 3:238297/1‑135 (MQ=255) cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCCCCCCCCCGGCCGGGGCGCCACTg > 2:11432/1‑135 (MQ=255) cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCACCTg > 5:270498/1‑135 (MQ=255) gAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGgcgccccggc > 3:348121/1‑129 (MQ=255) tCCGCCAGGGGGATGGTTGCATGACCGTCGTTCGTTTCCATAGGTGCATCGACGGCCAATGCCGCGATGCCCAGAGCGGCAAGACCTGCGCCCGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCg < 5:316003/135‑1 (MQ=255) gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCCACTGCCCGACg > 2:228066/1‑135 (MQ=255) gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGACg > 2:269867/1‑135 (MQ=255) gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCccgccg > 6:94380/1‑135 (MQ=255) gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACg > 4:110808/1‑135 (MQ=255) cAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGcc > 4:202572/1‑135 (MQ=255) ggATTGTTGCATCACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGAGGCCCAGAGCGCCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGa < 3:77294/135‑1 (MQ=255) aTTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAg < 4:252537/135‑1 (MQ=255) tggCATGACCCTGGTTGGTTTCCAGATTGGCATCGAGGGCCAATGGGGCTATGCCCAGAGCGGAAATACCTTCTACAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCg < 4:35561/132‑1 (MQ=255) tgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGCCGCCGATGAAGCCGAt > 8:118469/1‑135 (MQ=255) aCCCTGGTTCGTTCCCAGAGGTCCATCGCCGCCCAAGGGGGCGAGGCCCAGAGCGGCAAGACCTCCGCCAGCCCCACCCCGCCCCCCCCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAg < 7:308873/135‑1 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCATGCCTTGGCGCAACAGCCCCACGCCGATGAAGCCGAGTTGGAga > 2:66874/1‑134 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGGGCAACTGCCCGCCGCCGCTCAAGCCGCTGTGGGGc > 6:263171/1‑135 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGc < 6:80500/135‑1 (MQ=255) ttcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCTACTGCCCCACCCCGATCAAGCCGATGTGGAGCTGGCCg > 5:287575/1‑135 (MQ=255) ttcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCACGCCAGGGCGCCACAGCCCCCCCCCGCTGAAGCCCATGTGGCGCGGGCCg > 6:167155/1‑135 (MQ=255) tcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCAACTGCCCGCCGCCGCAGCAGCCGAAGTGGCGCGGGGCGt > 5:87941/1‑135 (MQ=255) cgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg > 3:182305/1‑73 (MQ=255) cgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCgg < 4:182305/73‑1 (MQ=255) cgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCTGGGCGCCAAGGCCCGCCGCCGATGACGCCGATGTGGAGCTGGccct > 6:44534/1‑132 (MQ=255) gttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTc < 4:15538/135‑1 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCCATGAAGCCGATGTGGAGCTGGCCGTTCAGgc > 8:308873/1‑135 (MQ=255) cAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACATCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGgcc > 8:358052/1‑135 (MQ=255) tgggCAACGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGccc < 4:50080/132‑1 (MQ=255) ggggCATCGACGGCCATGGGCGCGAGGCCCAGCGCGGCAACACCTGCGACAGCCTCAACCCGCCCCCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGccc < 4:187559/132‑1 (MQ=255) ttcATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGACTGGGCGCAACAGCCCGACGCCCATGAAGGCGAAGTGGCGCGGGCCGTTCAGGACCCCCAg > 2:304536/3‑135 (MQ=255) gaagccaCGGCCAAGGGCGCGATGCCCAGAGCGGCAACACCTTCGACCGCCGCACCCGGGCCCCCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGc < 8:342288/129‑1 (MQ=255) gCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTGGACAGCCTCAACCCGGCCACCGCCCAGGCGTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGc < 4:293953/135‑1 (MQ=255) cATAGACGGCCAATGGCGCGATCCCCAGAGCGGCAAGACCTTCGACAGCCTCAACACGCCCACCCCCCAGGCAGGGGCGCAAAAGGCCGCCACCCCAGAAGACGCAGATGAGCTGGCCGTTAAGGCCGCCCaccc > 6:349291/1‑132 (MQ=255) tCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCACCTGCCCGACGCCGCTGAAGCCGCTGTGGCGCTGGCCGTTGAGGCCGCCCCGCGTg > 8:200417/1‑135 (MQ=255) cGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCCACTGCCCGCCGCCGCGGAAGCCGCTGTGGGGCTGGCCGGTGAGGCCGCCCCGCGGGc > 5:167915/1‑135 (MQ=255) aaTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa < 2:301669/135‑1 (MQ=255) aaTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGACCCCGATGGGGAGCTGGGCGTTCAGGCCGCCCACCGTGCCGTCGAcc > 2:227964/1‑134 (MQ=255) cgATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCAGGGCGCCACTGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGGCCGGTCAGGCCGCCCAGCGGGCCGTCGCCAGCCAGGc > 2:129057/1‑135 (MQ=255) tGCCCAGCGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 8:282016/135‑1 (MQ=255) ggCCCAGCGGGCCAAGACCTTCCACCGCCTCAACCCGCCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 3:161196/134‑1 (MQ=255) ggCCCAGAGCGGCAAGACCTTCGCCAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 7:288356/134‑1 (MQ=255) cccAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGCTGCAGCCGATGTGGAGCTGGCCGTTAAGGCCGCCCCGCGTGCCTTCGCCAGCCAGGCGTGgc > 6:149196/1‑135 (MQ=255) agagCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCACGCCAGGCCGCAACTGCCCGCCGCCGCTGAAGCCGAAGTGGAGCTGGCCGGTGACGCCGCCCAGCCTGCCGTCGGCAGCCAGGCGTGGCgca > 2:109961/1‑135 (MQ=255) gcgCGGCACGACCTTCGACAGCCTCACCCCGCCCCCCCCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag < 2:215528/133‑1 (MQ=255) gCGGCAACCCCTCCCCCACCCTCACCCCGCCCCCCCCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCtcagca < 5:334521/135‑1 (MQ=255) aGACCTTCGACAGCCGCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCg < 2:322237/135‑1 (MQ=255) ctccGACAGCCGCACCCCGCCCCCCGCCCCGGCGGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAc < 1:249794/132‑1 (MQ=255) ttCGACAGCCTCAACCCGGCCACGGCCCTGGCGTGGGCGCAACTGCCCGACGCCGATGAAGCCGAGGGGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAcc < 8:193943/135‑1 (MQ=255) ttCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAcc < 8:122408/135‑1 (MQ=255) gccccccTCACCCCGCCCACCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAccgc < 3:24299/129‑1 (MQ=255) aCAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAATCCGATGTGGAGCTGGCCGTTCAGGCCGCCCCGCCTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAccgccc > 4:26552/1‑135 (MQ=255) aCAGCCTCAACCCGGCCACCGCCCACGCCTGGGCGCAACAGCCCCCCGCCCAGGAAGCCGATGTGGAGCTGGCCCTTCATGCCGCCCACCGTGCCGTATACACCAAAGCCTGGCCCACCAAAACAGCAAcccccc > 4:364297/1‑135 (MQ=255) gCCTCCACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 7:375047/135‑1 (MQ=255) gCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 2:6697/135‑1 (MQ=255) gCCCCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 1:45808/135‑1 (MQ=255) cAACCCGGCCACCGCCCAGGCCTGTGCGCAACTGccc > 4:356668/1‑37 (MQ=255) cAACCCGGCCACCGCCCAGGCCTGTGCGCAACTGccc < 3:356668/37‑1 (MQ=255) cAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGACGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCCCGGCac > 7:273975/1‑134 (MQ=255) | TGGACTTCCTCACCCAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAA > KT2440/4135458‑4135724 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |