Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,135,612	T→G	24.5%	W34G (TGG→GGG)	tgnC →	(Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,135,612	0	T	G	24.5%	63.2 / 4.5	69	W34G (TGG→GGG)	tgnC	(Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (22/24);  new base G (10/9);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 7.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTGGGTGA  >  KT2440/4135478‑4135745                                                                                                                                       |                                                                                                                                      cgaccgcTCACCGAATCCGCCACGGGGATTGTTGATTGACCGTCGTTCGTTTCCAGTTGTGCATCGTCGGCCAATGGCGCGATGCCCAGAGTGGCAAGACCTTCGAAAGCCTCAACCCGTCCACCTCCCAGGCCt                                                                                                                                       <  8:357915/129‑1 (MQ=255)   cACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGATCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGACTgg                                                                                                                                     >  1:54885/1‑135 (MQ=255)      ccTCACCGAATCCGCCAAGGGTTTTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCAGCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGgcg                                                                                                                                  <  8:7222/135‑1 (MQ=255)      ccTCACCGAATCCGCCAAGGGGATTGTTGCATGCCCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACCGCCTCAACCCGGCCACCGCCCAGGCCTGGgcg                                                                                                                                  >  6:189634/1‑135 (MQ=255)      ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCAGGgcg                                                                                                                                  >  2:184824/1‑135 (MQ=255)       cTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGCCCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGgcgc                                                                                                                                 >  3:238297/1‑135 (MQ=255)            cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCCCCCCCCCGGCCGGGGCGCCACTg                                                                                                                            >  2:11432/1‑135 (MQ=255)            cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCACCTg                                                                                                                            >  5:270498/1‑135 (MQ=255)             gAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGgcgccccggc                                                                                                                           >  3:348121/1‑129 (MQ=255)                tCCGCCAGGGGGATGGTTGCATGACCGTCGTTCGTTTCCATAGGTGCATCGACGGCCAATGCCGCGATGCCCAGAGCGGCAAGACCTGCGCCCGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCg                                                                                                                        <  5:316003/135‑1 (MQ=255)                   gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCCACTGCCCGACg                                                                                                                     >  2:228066/1‑135 (MQ=255)                   gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGACg                                                                                                                     >  2:269867/1‑135 (MQ=255)                   gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCccgccg                                                                                                                     >  6:94380/1‑135 (MQ=255)                   gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACg                                                                                                                     >  4:110808/1‑135 (MQ=255)                     cAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGcc                                                                                                                   >  4:202572/1‑135 (MQ=255)                          ggATTGTTGCATCACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGAGGCCCAGAGCGCCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGa                                                                                                              <  3:77294/135‑1 (MQ=255)                            aTTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAg                                                                                                            <  4:252537/135‑1 (MQ=255)                                tggCATGACCCTGGTTGGTTTCCAGATTGGCATCGAGGGCCAATGGGGCTATGCCCAGAGCGGAAATACCTTCTACAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCg                                                                                                         <  4:35561/132‑1 (MQ=255)                                 tgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGCCGCCGATGAAGCCGAt                                                                                                       >  8:118469/1‑135 (MQ=255)                                       aCCCTGGTTCGTTCCCAGAGGTCCATCGCCGCCCAAGGGGGCGAGGCCCAGAGCGGCAAGACCTCCGCCAGCCCCACCCCGCCCCCCCCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAg                                                                                                 <  7:308873/135‑1 (MQ=255)                                        cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCATGCCTTGGCGCAACAGCCCCACGCCGATGAAGCCGAGTTGGAga                                                                                                >  2:66874/1‑134 (MQ=255)                                        cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGGGCAACTGCCCGCCGCCGCTCAAGCCGCTGTGGGGc                                                                                                >  6:263171/1‑135 (MQ=255)                                        cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGc                                                                                                <  6:80500/135‑1 (MQ=255)                                              ttcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCTACTGCCCCACCCCGATCAAGCCGATGTGGAGCTGGCCg                                                                                          >  5:287575/1‑135 (MQ=255)                                              ttcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCACGCCAGGGCGCCACAGCCCCCCCCCGCTGAAGCCCATGTGGCGCGGGCCg                                                                                          >  6:167155/1‑135 (MQ=255)                                               tcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCGGGGCGCAACTGCCCGCCGCCGCAGCAGCCGAAGTGGCGCGGGGCGt                                                                                         >  5:87941/1‑135 (MQ=255)                                                cgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCTGGGCGCCAAGGCCCGCCGCCGATGACGCCGATGTGGAGCTGGccct                                                                                         >  6:44534/1‑132 (MQ=255)                                                 gttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTc                                                                                       <  4:15538/135‑1 (MQ=255)                                                     ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCCATGAAGCCGATGTGGAGCTGGCCGTTCAGgc                                                                                   >  8:308873/1‑135 (MQ=255)                                                      cAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACATCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGgcc                                                                                  >  8:358052/1‑135 (MQ=255)                                                          tgggCAACGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGccc                                                                              <  4:50080/132‑1 (MQ=255)                                                          ggggCATCGACGGCCATGGGCGCGAGGCCCAGCGCGGCAACACCTGCGACAGCCTCAACCCGCCCCCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGccc                                                                              <  4:187559/132‑1 (MQ=255)                                                            ttcATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGACTGGGCGCAACAGCCCGACGCCCATGAAGGCGAAGTGGCGCGGGCCGTTCAGGACCCCCAg                                                                            >  2:304536/3‑135 (MQ=255)                                                             gaagccaCGGCCAAGGGCGCGATGCCCAGAGCGGCAACACCTTCGACCGCCGCACCCGGGCCCCCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGc                                                                           <  8:342288/129‑1 (MQ=255)                                                             gCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTGGACAGCCTCAACCCGGCCACCGCCCAGGCGTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGc                                                                           <  4:293953/135‑1 (MQ=255)                                                              cATAGACGGCCAATGGCGCGATCCCCAGAGCGGCAAGACCTTCGACAGCCTCAACACGCCCACCCCCCAGGCAGGGGCGCAAAAGGCCGCCACCCCAGAAGACGCAGATGAGCTGGCCGTTAAGGCCGCCCaccc                                                                          >  6:349291/1‑132 (MQ=255)                                                                tCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCACCTGCCCGACGCCGCTGAAGCCGCTGTGGCGCTGGCCGTTGAGGCCGCCCCGCGTg                                                                        >  8:200417/1‑135 (MQ=255)                                                                 cGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCCACTGCCCGCCGCCGCGGAAGCCGCTGTGGGGCTGGCCGGTGAGGCCGCCCCGCGGGc                                                                       >  5:167915/1‑135 (MQ=255)                                                                         aaTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa                                                               <  2:301669/135‑1 (MQ=255)                                                                         aaTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGACCCCGATGGGGAGCTGGGCGTTCAGGCCGCCCACCGTGCCGTCGAcc                                                               >  2:227964/1‑134 (MQ=255)                                                                                cgATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCAGGGCGCCACTGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGGCCGGTCAGGCCGCCCAGCGGGCCGTCGCCAGCCAGGc                                                        >  2:129057/1‑135 (MQ=255)                                                                                   tGCCCAGCGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg                                                     <  8:282016/135‑1 (MQ=255)                                                                                   ggCCCAGCGGGCCAAGACCTTCCACCGCCTCAACCCGCCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg                                                     <  3:161196/134‑1 (MQ=255)                                                                                   ggCCCAGAGCGGCAAGACCTTCGCCAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg                                                     <  7:288356/134‑1 (MQ=255)                                                                                     cccAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGCTGCAGCCGATGTGGAGCTGGCCGTTAAGGCCGCCCCGCGTGCCTTCGCCAGCCAGGCGTGgc                                                   >  6:149196/1‑135 (MQ=255)                                                                                        agagCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCACGCCAGGCCGCAACTGCCCGCCGCCGCTGAAGCCGAAGTGGAGCTGGCCGGTGACGCCGCCCAGCCTGCCGTCGGCAGCCAGGCGTGGCgca                                                >  2:109961/1‑135 (MQ=255)                                                                                         gcgCGGCACGACCTTCGACAGCCTCACCCCGCCCCCCCCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag                                               <  2:215528/133‑1 (MQ=255)                                                                                           gCGGCAACCCCTCCCCCACCCTCACCCCGCCCCCCCCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCtcagca                                             <  5:334521/135‑1 (MQ=255)                                                                                                 aGACCTTCGACAGCCGCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCg                                       <  2:322237/135‑1 (MQ=255)                                                                                                     ctccGACAGCCGCACCCCGCCCCCCGCCCCGGCGGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAc                                   <  1:249794/132‑1 (MQ=255)                                                                                                      ttCGACAGCCTCAACCCGGCCACGGCCCTGGCGTGGGCGCAACTGCCCGACGCCGATGAAGCCGAGGGGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAcc                                  <  8:193943/135‑1 (MQ=255)                                                                                                      ttCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAcc                                  <  8:122408/135‑1 (MQ=255)                                                                                                         gccccccTCACCCCGCCCACCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAccgc                                <  3:24299/129‑1 (MQ=255)                                                                                                          aCAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAATCCGATGTGGAGCTGGCCGTTCAGGCCGCCCCGCCTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAccgccc                              >  4:26552/1‑135 (MQ=255)                                                                                                          aCAGCCTCAACCCGGCCACCGCCCACGCCTGGGCGCAACAGCCCCCCGCCCAGGAAGCCGATGTGGAGCTGGCCCTTCATGCCGCCCACCGTGCCGTATACACCAAAGCCTGGCCCACCAAAACAGCAAcccccc                              >  4:364297/1‑135 (MQ=255)                                                                                                             gCCTCCACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg                           <  7:375047/135‑1 (MQ=255)                                                                                                             gCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg                           <  2:6697/135‑1 (MQ=255)                                                                                                             gCCCCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg                           <  1:45808/135‑1 (MQ=255)                                                                                                                 cAACCCGGCCACCGCCCAGGCCTGTGCGCAACTGccc                                                                                                                         <  3:356668/37‑1 (MQ=255)                                                                                                                 cAACCCGGCCACCGCCCAGGCCTGTGCGCAACTGccc                                                                                                                         >  4:356668/1‑37 (MQ=255)                                                                                                                 cAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGACGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCCCGGCac                       >  7:273975/1‑134 (MQ=255)                                                                                                                     ccGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctg                   <  6:270498/135‑1 (MQ=255)                                                                                                                     ccGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctg                   <  8:109756/135‑1 (MQ=255)                                                                                                                     ccGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctg                   <  2:119112/135‑1 (MQ=255)                                                                                                                       ggCCACCGCCCTGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct                 <  5:236111/135‑1 (MQ=255)                                                                                                                       ggCCACCGCCCAGGCGTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct                 <  6:180592/135‑1 (MQ=255)                                                                                                                           aCCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTAAGGCCCCCCCGCGTGCCGTCGGCAGCAAGGCGTGGCGCAGCCTCACCGCTACCCCCCCCGGCCAGCTGCTGcgg             >  4:253465/1‑134 (MQ=255)                                                                                                                                 gctgCCTGGGGGCAACTGCGCGACGCCGATGAAGCCGTGGGTGAGCTGGCCGGTCAGGCCGCCCAGCGTGCCTTCGAGAGCTAGGCGTGGCGCAGCATCACCGGTAGCGCCCGCGGCAAGCTGCTGCGTCGTCTg       <  5:258176/132‑1 (MQ=255)                                                                                                                                  aGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCAGCTGCGTCGTCTgg      >  7:288930/1‑135 (MQ=255)                                                                                                                                      cTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTAAGGCCGCCCAGCGTGCCTTCGCCAGCAAGGCGTGGCGCAGCATCCCCGCTACCGCCCGCGGCCAGCTGCTGCGTCGTCTGGGTgc  >  8:66875/1‑134 (MQ=255)                                                                                                                                       |                                                                                                                                      CGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTGGGTGA  >  KT2440/4135478‑4135745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑