Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,245,134 A→C 22.5% L182V (TTG→GTG)  CJ019_03982 ← Crotonyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,245,1340AC22.5% 110.0 / 6.0 78L182V (TTG→GTG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (34/24);  new base C (4/16);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 3.95e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTGCGCCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245002‑4245265
                                                                                                                                    |                                                                                                                                    
gggTGCGCCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAAcc                                                                                                                                    <  3:122863/135‑1 (MQ=255)
gggTGCGCCGGGCGCCGTCGCGTATCAGCCGCGTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAAcc                                                                                                                                    <  3:295457/135‑1 (MQ=255)
     cgcCGGGCGCCGTCGCGTATCAGCCGCATGGTCAGGGCAAAGGCGAAGGTCGGGCCATGTGCCAGGCGCCCGGCCCCGTCCGCGGCCCCGCCCGGCAACAGCTCCGCCGCGCCGCCGTCGCTGACCCACCcgg‑cc                                                                                                                              >  6:11124/1‑134 (MQ=255)
        cGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGG‑CGGcc                                                                                                                           >  7:118502/1‑135 (MQ=255)
        cGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGcc                                                                                                                           >  7:122710/1‑135 (MQ=255)
                    cgTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGCCCCCCCCGG‑CGGCCCCGGCGCGCTCg                                                                                                               >  2:243042/1‑135 (MQ=255)
                              gCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAAATGCTCGTCGGCGCAGACTTCGCTGAACAACCCCG‑CGGCCAGGGCGCGATCGGCGccccccc                                                                                                     >  4:80357/1‑132 (MQ=255)
                                  ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGATCGGCGCCCcaccacac                                                                                                 >  6:118742/1‑133 (MQ=255)
                                  ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGCGCCCGATACGCGCCCCCCAAcag                                                                                                 >  2:52525/1‑133 (MQ=255)
                                   gtgggcgaATCGGCGATGGTGGGGCCTTGGGCCAGGGGCCGGGCATGTGCGGCGCAAACGGCAGGAAAGTGCTCGGCGGCCAAGACTACGGTGACCAACCCGG‑CGGCCTGGGCGCGAGGGGCGCCCCACAACtcg                                                                                                <  2:17082/131‑1 (MQ=255)
                                   gTCTGCGCATCGGCGATGGGCGGGCCATTGGCCAGGCGCCCGGCCAGGTCGGCGGCACGGGCAGGCAACTGCGCGGCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCTATGGGCGCCCCACAACtcg                                                                                                <  6:180632/135‑1 (MQ=255)
                                         gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGAg                                                                                           >  1:267474/1‑134 (MQ=255)
                                           aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGTCGCGGa                                                                                         >  1:310814/1‑134 (MQ=255)
                                           aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAggta                                                                                        >  4:317907/1‑133 (MQ=255)
                                           aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGAAACGCGCCCCCCAACGCGTCGAGGa                                                                                         >  3:115615/1‑134 (MQ=255)
                                           aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCCGGCGCGATCCGCGCCCCACAACTCGTCGAggc                                                                                         >  3:82670/1‑133 (MQ=255)
                                             tggCGATGGTGGGGCAATTGGCCTGGCGCGCGGCTTGTGCGGCGGCAACGGCAGGCAAGTGGTGGTCGGCGCAGAGTGCGCTGACCAACCGGG‑CGGCCTGGGGGCGATCGGGGCCCCACAACTCGTCGAGGAAca                                                                                      <  7:28374/134‑1 (MQ=255)
                                             aGGCTACGGGCGGGCCATGGCCCAGGCGCCCGGCCTGTTCGGCGGCAACGGCAGGCACCTGCGCGGCGGCGCAGACTTCGCTGACCACCCGGG‑CGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  4:98343/135‑1 (MQ=255)
                                             aGGCGATGGTCGGGCCTTTGGCCTGGCGCCGGGCCTGTTCGGGGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  2:251595/135‑1 (MQ=255)
                                             aGGCGATGGTCGGGCCATGGGCCCGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  8:347466/135‑1 (MQ=255)
                                              ggCGTAGGTCGGGCGATTGGCCTGGGGCGGGGCCTGTGCGGGGGCAAGGGCTGGCAAGTGCTCGTCGGCGCTGACTTGGCTGACCACCCGGG‑GGGCCTGGGCGGGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  8:315983/134‑1 (MQ=255)
                                              ggCGACGGGCGGCCCATGGCCCAGGCGCCCGGCCAGTGCGGCGGCACCGGCAGGCAACGGCGCGGCGGCGCAGACTGCGCTCACCACCCCGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  1:243042/134‑1 (MQ=255)
                                              ggCGAAGGGGGGGCAATTGGCAAGGCGCCCGGCCTGTTCGGCGGAAACGGCTGGCAACTGCGCGTCGGCGCTGACTTGGCTGACAAACCCGG‑CGGCCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  6:381950/134‑1 (MQ=255)
                                                cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCAACACGTCGAGGAAcagca                                                                                   >  1:266128/1‑135 (MQ=255)
                                                cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGCTCGGCGCCCCCCCACGCGTCGAGGAAcagca                                                                                   >  8:1026/1‑135 (MQ=255)
                                                 gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCACCCCGG‑CGGCCCGGGCGCGATCGGCGCCCCACCACACGTCGAGGAAcagcag                                                                                  >  6:196136/1‑135 (MQ=255)
                                                     gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCACGGGGCGCTCGGCGCCCCCCAACGCGTGGCGGGACACCAGgcgc                                                                              >  1:230545/1‑135 (MQ=255)
                                                        gggCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTg                                                                           >  8:372564/1‑135 (MQ=255)
                                                          gCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCCACACGTCGCGGCACCCCACgcgcgttgc                                                                         >  4:385698/1‑130 (MQ=255)
                                                            cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGGACAGCAGGCGCTTGGCCt                                                                       >  5:184465/1‑135 (MQ=255)
                                                            cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGAt                                                                                                                 >  8:244819/1‑93 (MQ=255)
                                                            cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGGACAGCCGGCGCTTGGCCt                                                                       >  8:254060/1‑135 (MQ=255)
                                                            cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCCGGCGCGCTAGGCGCCCCACAACTCGTCGAGGCACAGCAGGCGCGTGGccg                                                                       >  4:340003/1‑134 (MQ=255)
                                                            cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCCGGCGCGATCGGCGCCCCCCAACGCGGCGAGGCACAGCCGGCGCTTGGCCt                                                                       >  4:118480/1‑135 (MQ=255)
                                                            cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGAt                                                                                                                 <  7:244819/93‑1 (MQ=255)
                                                                  cccggCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGCACCGCCGGCGCTTGGCCTGCTCGc                                                                 >  3:153506/4‑135 (MQ=255)
                                                                  ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCAAGGCGCCCCCCCCCGCGTGGAGGCACACCCGGCGCGTGGCCGGCTCGc                                                                 >  1:10443/1‑135 (MQ=255)
                                                                         cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCACGGCGCGCTCGGCGCCCCCCCACACGTCGAGGCAAAGCCGGGGCGTTGGCGGGGCGCTGCCGa                                                           >  1:14380/1‑134 (MQ=255)
                                                                              ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCCACACGTCGGGGGCCCCCCGGCGCGTGGCCGGCGAGCAGCCGaaagagc                                                     >  2:311072/1‑129 (MQ=255)
                                                                              ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGCACAGCAGGCGCTTGGCCTGCTCGCTGCCCATCCGcc                                                     >  6:56383/1‑135 (MQ=255)
                                                                                tgtgcggcggCACCGGCCGGAAACTGCGCGGCGGCGCAGAGTTCGCGCACCACCCCGG‑CGGCCAGGGCGGGAGCGGGGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  6:349770/131‑1 (MQ=255)
                                                                                aGTGCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTCACCAACCCGG‑CGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  7:327776/135‑1 (MQ=255)
                                                                                          cAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCAACTCGTCGCGGAACACCAGGCGCGTGGCCTGCTCGCGGCCGCTCACCCGGGGCCGGTGcc                                         >  7:286057/1‑135 (MQ=255)
                                                                                           aaCGCCAGCCAACTGCGCGGCGGCGCAGACTGCGCGCACCAACCCGG‑CGCCCCGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCa                                        <  8:118502/135‑1 (MQ=255)
                                                                                               ggaggcaACTGCTCTCCGGCGCAGAGTTCGCTGACCACCCGGG‑CGGCCAGGGCGCGTGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTg                                    <  7:135587/133‑1 (MQ=255)
                                                                                               gcaggcaACGGCGCGGCGGCGAAGAGTGCGCTCACCACCCCGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTg                                    <  7:182431/135‑1 (MQ=255)
                                                                                                 agccaACTGGTGGCCGGCCCCGACTGCGGCGACCACCCGGG‑CGCCCGGGGCGGGATCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCATGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                  <  4:15193/132‑1 (MQ=255)
                                                                                                        tGCTCGTGGGCGCAGACTTCGCTCACCAACCCGG‑CGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  2:298144/135‑1 (MQ=255)
                                                                                                        tGCTCGTCGCCGCAGACTGCGCTCCCCAACCCGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  2:10443/135‑1 (MQ=255)
                                                                                                        tGCGCGGCGGCGCAGACGTCGCGGCCCAACCGGG‑CGGCCGGGGCGCGCGGGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  7:310588/135‑1 (MQ=255)
                                                                                                           tcgtcgGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCg                        >  8:231617/1‑135 (MQ=255)
                                                                                                             ggcgGCGCAGAGTGCGCAGACCACCCCGG‑CGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                      <  3:24887/133‑1 (MQ=255)
                                                                                                             ggcgGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                      <  6:279877/133‑1 (MQ=255)
                                                                                                               cgGCCCAGACTGCGCTGCCCAACCCGG‑GGGCCCGGGCCGGTGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCGCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGc                    <  5:118742/135‑1 (MQ=255)
                                                                                                                ggCGCAGACTTCGCTGACCACCCCGG‑CGGCCCGGGCGCGATCGGCGCCCCACAACACGTCGAGGAACAGCACGCGCTTGGCCAGCTCGCGGCCGCTCACCCGTGGCAGGGGCCAGCAGGCGCCGGCCGCGGGGcg                   >  6:386376/1‑134 (MQ=255)
                                                                                                                ggCGCAGACTCCGCTGCCCACCCCGC‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGCGGAACAGCAGGCGCGTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCt                   <  5:372516/135‑1 (MQ=255)
                                                                                                                  cgcAGACTTCGCTGACCACCCCGG‑GGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                 <  4:108344/135‑1 (MQ=255)
                                                                                                                  cgcAGACTTCGCTGACCACCCCGC‑CGGCCCGGGCGCGATCGGCGCCCCCCAACACCGCCAGGCACCCCCGGCGCATGGCCAGCTCGCAGCCCATCAGCCGTGGCACGTGGCCGCGTGCGCCCGCATCGGGGcg                   >  2:256423/1‑132 (MQ=255)
                                                                                                                  cgcAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGATCGGGGCCCCACAACTCGGCGAGGAACATCAGGCGCTTGGCCTGCTCGCTGCCGAGCCGCCCGGGCCGGTGCCCGCTGGCGCCGGCCTCGGGGCt                   >  1:26581/1‑133 (MQ=255)
                                                                                                                  cgcAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGCTCGGCGCCCCACAACTCGGCGAGGAACAGCCGGCGCTTGGCCTGCTCGCTGCCGCTCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCt                   >  7:253603/1‑133 (MQ=255)
                                                                                                                  cgcAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                 <  2:310814/135‑1 (MQ=255)
                                                                                                                   gcAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                <  4:201589/135‑1 (MQ=255)
                                                                                                                   gcAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                <  6:93587/135‑1 (MQ=255)
                                                                                                                   ccAGACTGCGCGCCCCACCCGGG‑CGGCCGGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                <  8:336471/134‑1 (MQ=255)
                                                                                                                       aCTTCGCTGCCCAACCCGG‑CGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc            <  6:193069/135‑1 (MQ=255)
                                                                                                                       aCTTCGCTGACCACCCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc            <  6:162406/135‑1 (MQ=255)
                                                                                                                       aCTTCGCTGACCAACCCGG‑CGGCCCGGGCGCCATCGGCGCCCCCCCACTCGTCGAGGCACAGCAGGCGCTTGGCCGTCTCCCTGCCGATCAGCCCGGGCAGGTGCCCGCTGGCGCCGGCCTCGGGGCGGGAGccc            >  7:101359/1‑135 (MQ=255)
                                                                                                                       aCTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGCTCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCCTCGGGGCTGTTGccc            >  3:105066/1‑135 (MQ=255)
                                                                                                                       aCTGCGCTCACCCCCCCGG‑CGCCCGGGGCGCGCGGGGCCCCCCACAACTCGTCGAGGAACAGCCGGCGCTTGGCCGGCGCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc            <  6:371871/135‑1 (MQ=255)
                                                                                                                       aCTCCGCTCACCACCCGGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc            <  3:385698/135‑1 (MQ=255)
                                                                                                                              tGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGTACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCggg     >  5:141105/1‑135 (MQ=255)
                                                                                                                               gACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGCGGGACACCCGGCGCTTGGCCGGCTCGCTGCCGCTCACCCGGGGGCGGTGCCAGCGGGCGCCGGCATCGGGGCTGTAGCCCAAGCTggg    >  4:251237/1‑134 (MQ=255)
                                                                                                                                 cccaCCGGG‑GGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCATCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  2:3232/132‑1 (MQ=255)
                                                                                                                                 ccACCCCGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  7:81388/135‑1 (MQ=255)
                                                                                                                                 ccACCCCGG‑CGCCCCGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  3:278206/135‑1 (MQ=255)
                                                                                                                                 ccAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  2:266128/135‑1 (MQ=255)
                                                                                                                                 ccAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  2:322251/135‑1 (MQ=255)
                                                                                                                                 ccAACCCGG‑CGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  6:184465/135‑1 (MQ=255)
                                                                                                                                 ccAACCCGG‑CGACCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  8:215704/135‑1 (MQ=255)
                                                                                                                                  ccaCCCGGCCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGGCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  2:157837/133‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
GGGTGCGCCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245002‑4245265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑