Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,500,158 T→C 6.2% L64P (CTG→CCG)  yqjG → Glutathionyl‑hydroquinone reductase YqjG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,500,1580TC 6.2% 198.3 / 3.2 65L64P (CTG→CCG) yqjGGlutathionyl‑hydroquinone reductase YqjG
Reads supporting (aligned to +/- strand):  ref base T (24/35);  new base C (2/2);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

GGCACGTTCAAGCGGGAGAATGCCCAGCGCCGTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTACACTCAGGAT  >  KT2440/4500025‑4500291
                                                                                                                                     |                                                                                                                                     
ggCACGTTCAAGCGGGAGAATGCCCAGCGCCGTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTg                                                                                                                                      <  4:93188/135‑1 (MQ=255)
    cGTTCAAGCGGGAGAATGCCCAGCGCCGTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGc                                                                                                                                  >  6:304769/1‑135 (MQ=255)
          aGCGGGAGAATGCCCAGCGCCGTAATCAGCTGCCCGCCCCCGATGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGCTATTTCGCGCTCTGAAAGGCCTGGAGCCGtgtc                                                                                                                            >  8:340016/1‑132 (MQ=255)
           gCGGGAGAATGCCCAGCGCCGTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCCCCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGAt                                                                                                                           >  3:220875/1‑135 (MQ=255)
           gCGGGAGAATGCCCAGCGCCGTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGAt                                                                                                                           <  7:280233/135‑1 (MQ=255)
                   aTGCCCAGCGCCGTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACgtg                                                                                                                    >  2:20417/1‑134 (MQ=255)
                            gcCGTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCCCCCTGATCTTTCGCGCGCTGAAAGGCCCGGAGCCGTTGATCGACGTGTCAGTGGTCa                                                                                                          >  3:316875/1‑135 (MQ=255)
                            gcCGTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCCGGAGCCGTTGATCGACGTGTCAGTGGTCa                                                                                                          >  3:311657/1‑135 (MQ=255)
                               gTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTGAGTGGTCAgct                                                                                                       <  7:276804/135‑1 (MQ=255)
                               gTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAgct                                                                                                       <  4:14871/135‑1 (MQ=255)
                               gTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAgct                                                                                                       <  1:224703/135‑1 (MQ=255)
                               gTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAgct                                                                                                       <  3:15320/135‑1 (MQ=255)
                                   tCAGCTGCCCGCCCCCGAGGCCGGCCGCTCCCACCTGTATGTGTCGCTGGCCTGCCGCTGGGCCCACCGCCCCCTCATCTTGCGCGCGCTGAAAGGCAGGGAGCCGTTGATCGGCCGGTAAGTGTTCCgctggc                                                                                                    >  6:217638/1‑134 (MQ=255)
                                    cAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCTCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGGCAgctggctg                                                                                                  <  8:281302/135‑1 (MQ=255)
                                        tGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGt                                                                                                            <  6:171855/121‑1 (MQ=255)
                                        tGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGt                                                                                                            >  5:171855/1‑121 (MQ=255)
                                           ccgcccCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAg                                                                                            <  7:258256/134‑1 (MQ=255)
                                             gcccCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGGGTCAGTGGTCAGCTGGCTGATGCAGGAc                                                                                         >  7:369233/1‑135 (MQ=255)
                                                            cgCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTGGTTCGACGGGTCAGTGGTCAGCTGGCTGATGCAGGGCCCTGGCTGGGCCTTc                                                                          >  2:303455/1‑135 (MQ=255)
                                                                  caccTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATcag                                                                    <  5:306806/135‑1 (MQ=255)
                                                                  caccTGTATGTGTCGCTGGCCTGCCCACGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATcag                                                                    <  4:379640/135‑1 (MQ=255)
                                                                    ccTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATcagca                                                                  <  2:238573/135‑1 (MQ=255)
                                                                    ccTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATcagca                                                                  <  5:289348/135‑1 (MQ=255)
                                                                         aTGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAg                                                             <  7:74728/135‑1 (MQ=255)
                                                                         aTGTGTCGCTGGCCTGCCCATGCGCCCACCGCACCCTGATCTTTCGCGCGCTGAACGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAg                                                             <  5:99771/135‑1 (MQ=255)
                                                                           gtgtCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATAAGCAGCAAGGc                                                           >  7:389953/1‑135 (MQ=255)
                                                                           gtggCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCGGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAgg                                                            <  5:290674/134‑1 (MQ=255)
                                                                             gtCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAACGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCt                                                          <  1:378198/134‑1 (MQ=255)
                                                                               cGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTcc                                                        <  1:254839/134‑1 (MQ=255)
                                                                                 cTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGt                                                     >  3:329073/1‑135 (MQ=255)
                                                                                 cTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGt                                                     <  8:247733/135‑1 (MQ=255)
                                                                                 cTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCATGGAGCCGTGGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGGCCATGGCTGGCCCGTCGATCAGCCGCAAGGCTCCAGt                                                     >  3:242974/1‑135 (MQ=255)
                                                                                  tGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGGCTGTGGTCAGCTGGCTGATGCCGGACCATGGCTGGTCCTTCGATCGGCAGGAAGGCTcacgtg                                                    >  2:253013/1‑130 (MQ=255)
                                                                                               ggCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGa                                       <  5:214638/135‑1 (MQ=255)
                                                                                                      cGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGCCCGTCGATCAGCAGCAAGGCTCCAGTGGGGCCCACCTCGACGCCCTg                                >  5:161326/1‑135 (MQ=255)
                                                                                                      cGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTggg                                                  >  6:89459/1‑117 (MQ=255)
                                                                                                      cGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTggg                                                  <  5:89459/117‑1 (MQ=255)
                                                                                                      cGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCt                                 >  5:339903/1‑134 (MQ=255)
                                                                                                       gCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGCCGTGGCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCTCCGCCCAGc                               >  4:271826/1‑135 (MQ=255)
                                                                                                       gCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGc                               >  6:382170/1‑135 (MQ=255)
                                                                                                        cACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATcagca                                                                  <  8:113374/99‑1 (MQ=255)
                                                                                                        cACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATcagca                                                                  >  7:113374/1‑99 (MQ=255)
                                                                                                         aCCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAg                             <  4:68476/135‑1 (MQ=255)
                                                                                                          cccTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGt                            <  1:207046/135‑1 (MQ=255)
                                                                                                           ccTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTa                           >  6:360592/1‑135 (MQ=255)
                                                                                                                tCTTTGGCGCGCTGAAAGGCCTGGAGGCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTg                       <  5:217638/134‑1 (MQ=255)
                                                                                                                tCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTg                       <  3:271826/134‑1 (MQ=255)
                                                                                                                tCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGAGCGACGTGGCAGTGGTCAGCTGGCTGATGCAGGACCAGGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTg                       <  6:83577/134‑1 (MQ=255)
                                                                                                                  tttCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTTGCCCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCAACTCGACGCCCTGCAGTACCTGCAc                    >  6:12249/1‑135 (MQ=255)
                                                                                                                  tttCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGCCCACCTCGACGCCCTGCAGTACCTGCAc                    >  1:29243/1‑135 (MQ=255)
                                                                                                                  tttCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCAc                    >  5:379159/1‑135 (MQ=255)
                                                                                                                       cgcgcTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAgcg               >  7:354387/1‑135 (MQ=255)
                                                                                                                       cgcgcTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAgc                <  8:74316/134‑1 (MQ=255)
                                                                                                                        gcgcTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCGTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGCCGCCCTTCAGTACCTGCCCCAgcg               >  6:332110/1‑134 (MQ=255)
                                                                                                                          gcTGAAAGGCCTGGAGCCGTTGATCGGCGTGTCACTTGTAAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGAACAGCAGCAACCCTCCAGTGGGGACCACCGCGACGCCCAGCAGAACCTCCACCCgcgcga            >  5:345026/1‑133 (MQ=255)
                                                                                                                           cTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTac           <  8:94988/135‑1 (MQ=255)
                                                                                                                               aaGGCCCGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTACACTc       <  4:311657/135‑1 (MQ=255)
                                                                                                                               aaGGCCCGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTACACTc       <  4:316875/135‑1 (MQ=255)
                                                                                                                                  gCCTGGAGCCGTTGATCGACGTGGCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTACACTCAgg    <  4:220875/135‑1 (MQ=255)
                                                                                                                                    cTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGTGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTACACTCAGGAt  <  2:355292/135‑1 (MQ=255)
                                                                                                                                    cTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTACACTCAGGAt  <  6:339903/135‑1 (MQ=255)
                                                                                                                                    cTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTACACTCAGGAt  <  1:303455/135‑1 (MQ=255)
                                                                                                                                    cTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTACACTCAGGAt  <  4:179347/135‑1 (MQ=255)
                                                                                                                                    cTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTACACTCAGGAt  <  6:132385/135‑1 (MQ=255)
                                                                                                                                    cTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTACACTCAGGAt  <  5:360592/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
GGCACGTTCAAGCGGGAGAATGCCCAGCGCCGTAATCAGCTGCCCGCCCCCGAGGCCGGCCGCTACCACCTGTATGTGTCGCTGGCCTGCCCATGGGCCCACCGCACCCTGATCTTTCGCGCGCTGAAAGGCCTGGAGCCGTTGATCGACGTGTCAGTGGTCAGCTGGCTGATGCAGGACCATGGCTGGACCTTCGATCAGCAGCAAGGCTCCAGTGGGGACCACCTCGACGCCCTGCAGTACCTGCACCAGCGCTACACTCAGGAT  >  KT2440/4500025‑4500291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑