Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,656,288 A→C 16.6% D502A (GAC→GCC)  nuoG → NADH‑quinone oxidoreductase subunit G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,656,2880AC16.6% 86.9 / 2.6 51D502A (GAC→GCC) nuoGNADH‑quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base A (27/14);  new base C (6/4);  total (33/18)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGCCATCGACCCGAGCGCCCCTGCCGTCGCCGGCCTGGACGACGAAGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGAT  >  KT2440/4656158‑4656421
                                                                                                                                  |                                                                                                                                     
cACGCCATCGACCCGAGCGCCCCTGCCGTCGCCGGCCTGGACGCCGCAGCCCCGGCCCTGGCCCCGCGCCTCGCCGCCGCCCGGGTCGCCGCCAAGCGCCCCCGGGGCGGGGCCGGTGCTTAGCTGGCCGCCCCg                                                                                                                                   >  1:312079/1‑135 (MQ=255)
cACGCCATCGACCCGAGCGCCCCTGCCGGCGCCGGCCTGGACGACGAAGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCg                                                                                                                                   <  8:116548/135‑1 (MQ=255)
                  gcCCCTGCCGTCGCCGGCCTGGACGACGAAGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGCAGCGGCt                                                                                                                 >  3:247483/1‑135 (MQ=255)
                  gcCCCTGCCGTCGCCCGCCTGGACGACGAAGCCCACGCCCCGGCCCAGCGCCTCGCCGACCCCCAGGTAGCCGCCAAGCCCCCCCGGGTCGTAGCCGGTGCTTCGCTGGCCGCCCCCGCGCGGCTCGCAGCGGca                                                                                                                 >  6:329374/1‑134 (MQ=255)
                     ccTGCCGTCGCCGGCCTGGACGACGAAGCCCAGGCCCTGGCCCAGCGCAGCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGcc                                                                                                              <  2:127601/135‑1 (MQ=255)
                            tCGCCGGCCTGGACGACGAAGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCg                                                                                                       <  5:289418/135‑1 (MQ=255)
                            tCGCCGGCCTGGACGACGAAGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCg                                                                                                       <  8:364882/135‑1 (MQ=255)
                            tCGCCGGCCTGGACGACGAAGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCg                                                                                                       <  4:255008/135‑1 (MQ=255)
                                 ggCCTGGCCGCCGAAGCCCAGGCCCTGGCCCCGCGCATCGCCGCCGCCCTGGTCGCCGCCAATCGCCCCCGGGTCGTTGCCGGTACTTCGCTGGCCGCCCCGGCGCTGATCGAAGCGGCTGCCAACCTCGCCAAg                                                                                                  >  4:237571/1‑135 (MQ=255)
                                 ggCCTGGACGCCGAAGCCCAGGCCCTGGCCCAGCGCATCGCCGCCGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGCTCGAAGCGGCTGCCAACATCGCCAAg                                                                                                  >  2:47429/1‑135 (MQ=255)
                                 ggCCTGGACGACGAAGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGCTCGAAGCGGCTGCCAACATCGCCAAg                                                                                                  >  8:94217/1‑135 (MQ=255)
                                 ggCCTGGACGACGAAGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTAGCTGGCCGACCCGGCGCTGCTCGAAGCGGCTGCCAACATCGCCAAg                                                                                                  >  6:184363/1‑135 (MQ=255)
                                 ggCCTGGACGACGAAGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACAGGTCGTAGCCGGTACTTCGCTGGCCGACCCCGCGCGGATCGAAGCGGCGGCCAACATCCCCAAg                                                                                                  >  5:371071/1‑135 (MQ=255)
                                               aGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGc                                                                                                      >  6:223397/1‑117 (MQ=255)
                                               aGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGc                                                                                                      <  5:223397/117‑1 (MQ=255)
                                                    aGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGAAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCAGCCAACATCGCCAAGGCCCTGAAGCTGCGCgag                                                                                >  3:277092/1‑134 (MQ=255)
                                                        ccTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGCAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGaaaaa                                                                           >  7:252254/1‑135 (MQ=255)
                                                            gCCCAGCGCATCGCCGACGCCCTGGGCGCCGCCAAGCGCCCACTGGGCGTAGCCGGTACTTCGCGGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCACGGCCCTGAAGCTGCGCGAGAAAAACGGc                                                                       <  2:39516/135‑1 (MQ=255)
                                                              ccAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACAGGTCCTAGGCGGTACTTCGCTGGCCGACCCGGCGCTGATCGCAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTc                                                                     >  8:67151/1‑135 (MQ=255)
                                                              ccAGCGCATCGCCGACGCCCTGGTCGCAGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGCCCCGGCGCTGCTCGAAGCCGCAGCCCACCTCGCCAAGGCCCTGCACCTGCGCGAGAAAAACGGATc                                                                     >  7:283975/1‑135 (MQ=255)
                                                                   gcATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGa                                                                >  1:58544/1‑135 (MQ=255)
                                                                    cATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAg                                                               >  4:351961/1‑135 (MQ=255)
                                                                     aTCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGc                                                              >  7:361559/1‑135 (MQ=255)
                                                                     aTCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGc                                                              >  7:264924/1‑135 (MQ=255)
                                                                         ccGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGCCCCGGCGCTGATCGACGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCtgg                                                          >  3:134189/1‑135 (MQ=255)
                                                                          cgccGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGCCCCGGCGCTGATCGACGCGGCTGCCCACATCGCCAAGGCGCTGAAGCTGGGCGCGGAAAACGGCTCGCTGAGCCtggt                                                         >  5:217178/4‑135 (MQ=255)
                                                                          cGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCTCTGGCCGACCCGGCGCTGATCGATGCGGCTGCCAACATCGCCAAGGCCCTGACGCTGCGCGAGATAAACGGCGCGCTGAGCCtggt                                                         >  7:326607/1‑135 (MQ=255)
                                                                          cGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCCACATCGCCAAGGCCCTGCAGCTGCGCGAGAAAAACGGCTCGCTGAGCCtggt                                                         >  6:221765/1‑135 (MQ=255)
                                                                          cGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCGGATCGCAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCtggt                                                         >  7:182419/1‑135 (MQ=255)
                                                                               cccTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCACCATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGc                                                    >  7:302251/1‑135 (MQ=255)
                                                                               cccTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGc                                                    >  8:22278/1‑135 (MQ=255)
                                                                                     tCGCCGCCAAGCGCCCACTGGTCGTCGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGACGCGGCTGCCAACATCGCCACGCCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAgg                                              <  6:296797/135‑1 (MQ=255)
                                                                                      cgccgcCACGCGCCCCCTGGTCGTCGCCGGCACTGCGCTGGCCGACCCGGCGCTGATCGACGCGGCGGCCAACATCGCCAAGGCCCTGAAGCTGCGCGACAAACACGGCGCGCTGAGCCTGGTGGTGCCTGAGGc                                             <  2:48710/135‑1 (MQ=255)
                                                                                      cgccgcCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAgg                                              >  4:28492/1‑134 (MQ=255)
                                                                                           ccccccgcCCCCTGGTCGCCGCCGGTACTCGGCGCGCCGCCCGGGGGCTGCTCCACCCGGCGGCCAACTACCCCACGGCCCTGACGCGGCGCTACAAACACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACa                                        <  8:142611/130‑1 (MQ=255)
                                                                                                cgcCCAGTGGGCGTAGCCGGTACTTCGCTGGCCGCCCCGGCGCTGACCGACGCGGCGGCCAACATCGCCACGGCCCTGAACCTGGGCGAGAAACACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTc                                   <  5:147033/135‑1 (MQ=255)
                                                                                                        gggggTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGCAGCGGCTGCCAACATCGCCACGCCCCTGAAGCTGCCCTAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGc                           <  3:148772/131‑1 (MQ=255)
                                                                                                                  ggTACTTCGCTGGCCGACCCGGCGCTGCTCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCCACAGCCTCGGCCGGGCCCTGCTCCGt                 >  1:369108/1‑135 (MQ=255)
                                                                                                                     aCTCCGCGGGCCCCCCCGGCGCTTATCGACGCGGCGGCCAACATCCCCACGGCCCTCACGCGGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGGGGc              <  1:321177/135‑1 (MQ=255)
                                                                                                                      cTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTgg               >  6:266331/1‑133 (MQ=255)
                                                                                                                      cTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTgg               >  7:80969/1‑133 (MQ=255)
                                                                                                                            tGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCCACAGCCTCGGCCTGGCCATGCTCGGTGGCGAgtcgg       >  5:237314/1‑135 (MQ=255)
                                                                                                                              gCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAgtcggt      >  7:304777/1‑134 (MQ=255)
                                                                                                                               ccGCCCCGGCGGTGATCGACGCGGCTGCCAACATCGCCACGGCCCTGACGCTGCGCGATAAACACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAgtcggtcg    <  6:371071/135‑1 (MQ=255)
                                                                                                                               ccGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAgtcggtcg    <  8:304777/135‑1 (MQ=255)
                                                                                                                               ccGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAgtcggtcg    >  4:139072/1‑135 (MQ=255)
                                                                                                                               ccGACCCGGCGCTGAGCGAAGCGGCTGCCAACATCGCCAAGGCCCTGACGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAgtcggtcg    <  2:273742/135‑1 (MQ=255)
                                                                                                                                cGACCCGGCGCTGATCGCAGCGGCGGCCAACACCGCCACGGCCCTGAAGCTCCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGa   <  2:88785/135‑1 (MQ=255)
                                                                                                                                cGACCCGGCGCTGATCGACGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGa   <  6:284720/135‑1 (MQ=255)
                                                                                                                                cGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGa   >  1:295436/1‑135 (MQ=255)
                                                                                                                                 gACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGAt  >  3:235667/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                                     
CACGCCATCGACCCGAGCGCCCCTGCCGTCGCCGGCCTGGACGACGAAGCCCAGGCCCTGGCCCAGCGCATCGCCGACGCCCTGGTCGCCGCCAAGCGCCCACTGGTCGTAGCCGGTACTTCGCTGGCCGACCCGGCGCTGATCGAAGCGGCTGCCAACATCGCCAAGGCCCTGAAGCTGCGCGAGAAAAACGGCTCGCTGAGCCTGGTGGTGCCTGAGGCCAACAGCCTCGGCCTGGCCATGCTCGGTGGCGAGTCGGTCGAT  >  KT2440/4656158‑4656421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑